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The map label for this gene is thiF [H]

Identifier: 152977086

GI number: 152977086

Start: 3459925

End: 3460938

Strand: Reverse

Name: thiF [H]

Synonym: Bcer98_3396

Alternate gene names: 152977086

Gene position: 3460938-3459925 (Counterclockwise)

Preceding gene: 152977087

Following gene: 152977084

Centisome position: 84.68

GC content: 39.05

Gene sequence:

>1014_bases
TTGCAGGACCGATATTCAAGGCAAATGTTATTTTCAAGTATTGGTGAAGATGGACAGAGGAAAATAAGAGAAAAGCATGT
ATTGCTTATTGGTGCTGGAGCTCTTGGTGCCGCCAATGCTGAAGCTCTTGCTAGAGCAGGAATCGGAAAATTGACAATTG
CGGATCGAGATTATGTCGAGTGGAGCAATTTGCAGCGGCAGCAATTATATACAGAAGAAGATGCCAAGCAATATAAGCCA
AAGGCGATTGCAGCTGCAGAACATTTACAGAAGATTAATTCTGAAGTTGAGATTATTCCAGTTGTGACAGATGTGGCGAT
ACAAGAGATGGAAGATTTCATTCAAGGTATAGATTTAATAATAGATGCAACAGATAATTTTGATACGCGTCTTCTTATTA
ATGATATTTCACAAAAATATAATGTACCTTGGATATATGGAGGATGTGTTGGTAGTTATGGCGTAACGTATACAATTCTT
CCAGGAAAAACACCGTGCTTTCGCTGTTTGATGGAGCATCCGACAAGCGGTGCCACATGTGATACGGCAGGGGTAATACA
GCCGGCGGTACAAATGGTCGTTGCACATCAAGTAACCGAAGCTTTAAAAATATTAGTAGAAGATTTTGGAGCACTTCGGG
AGACAATGTTGTCATTTGATGTTTGGAATAATCAACATATGGCATTTAAAGTGAATAGGCAGAAAAAAGAGAGCTGCCCA
TCTTGTGGCAAGTTACGTACGTACCCAAGTTTGGCATTTGAATCACAAATGAAAACCGAAGTATTATGTGGGCGAAATAC
GGTTCAAATCCGTCCTGGCGTTTCGAAATCTCTTAATCTAGAAGAAATTAAAAAGCGCTTACAAAAAAACATGGATATTC
AAGTAACACCGTATTTATTATCCTTTTTCATCGAGGAGTATCGTTTTGTTCTCTTTAAGGATGGTAGAGCGTTTGTTCAT
GGGACAAATGATAAGGCAGAAGCGAAGCGATTATATACAAGGTATATAGGCTAA

Upstream 100 bases:

>100_bases
CGAAAAGACCGATATTCTGATGAGCGGACGGAAGAAAGCGCGAAAAATCGTGCGAAAATTGAAATGTCTTATATTGGAGG
ATAAGAAGGGAGGGAAAACA

Downstream 100 bases:

>100_bases
ACCTAAAGGCGACCTTTAGGTTTAGCGGATGCGTTATATTTATGAATTTCGAAGATAGTCGCCGCTTTTTCCTCCTGTTT
TTTCGAGAAGATATGTTTCA

Product: thiamine/molybdopterin biosynthesis MoeB-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MQDRYSRQMLFSSIGEDGQRKIREKHVLLIGAGALGAANAEALARAGIGKLTIADRDYVEWSNLQRQQLYTEEDAKQYKP
KAIAAAEHLQKINSEVEIIPVVTDVAIQEMEDFIQGIDLIIDATDNFDTRLLINDISQKYNVPWIYGGCVGSYGVTYTIL
PGKTPCFRCLMEHPTSGATCDTAGVIQPAVQMVVAHQVTEALKILVEDFGALRETMLSFDVWNNQHMAFKVNRQKKESCP
SCGKLRTYPSLAFESQMKTEVLCGRNTVQIRPGVSKSLNLEEIKKRLQKNMDIQVTPYLLSFFIEEYRFVLFKDGRAFVH
GTNDKAEAKRLYTRYIG

Sequences:

>Translated_337_residues
MQDRYSRQMLFSSIGEDGQRKIREKHVLLIGAGALGAANAEALARAGIGKLTIADRDYVEWSNLQRQQLYTEEDAKQYKP
KAIAAAEHLQKINSEVEIIPVVTDVAIQEMEDFIQGIDLIIDATDNFDTRLLINDISQKYNVPWIYGGCVGSYGVTYTIL
PGKTPCFRCLMEHPTSGATCDTAGVIQPAVQMVVAHQVTEALKILVEDFGALRETMLSFDVWNNQHMAFKVNRQKKESCP
SCGKLRTYPSLAFESQMKTEVLCGRNTVQIRPGVSKSLNLEEIKKRLQKNMDIQVTPYLLSFFIEEYRFVLFKDGRAFVH
GTNDKAEAKRLYTRYIG
>Mature_337_residues
MQDRYSRQMLFSSIGEDGQRKIREKHVLLIGAGALGAANAEALARAGIGKLTIADRDYVEWSNLQRQQLYTEEDAKQYKP
KAIAAAEHLQKINSEVEIIPVVTDVAIQEMEDFIQGIDLIIDATDNFDTRLLINDISQKYNVPWIYGGCVGSYGVTYTIL
PGKTPCFRCLMEHPTSGATCDTAGVIQPAVQMVVAHQVTEALKILVEDFGALRETMLSFDVWNNQHMAFKVNRQKKESCP
SCGKLRTYPSLAFESQMKTEVLCGRNTVQIRPGVSKSLNLEEIKKRLQKNMDIQVTPYLLSFFIEEYRFVLFKDGRAFVH
GTNDKAEAKRLYTRYIG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=267, Percent_Identity=31.0861423220974, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI38045948, Length=170, Percent_Identity=30.5882352941176, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI23510338, Length=182, Percent_Identity=28.021978021978, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI23510340, Length=182, Percent_Identity=28.021978021978, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI150417996, Length=182, Percent_Identity=29.1208791208791, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI38045942, Length=159, Percent_Identity=32.7044025157233, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI38045944, Length=159, Percent_Identity=32.7044025157233, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI13376212, Length=181, Percent_Identity=32.5966850828729, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787048, Length=243, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=35.3413654618474, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17540406, Length=246, Percent_Identity=32.1138211382114, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI193203301, Length=155, Percent_Identity=30.3225806451613, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508819, Length=182, Percent_Identity=32.967032967033, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=322, Percent_Identity=29.5031055900621, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320598, Length=175, Percent_Identity=35.4285714285714, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322639, Length=185, Percent_Identity=27.5675675675676, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6325323, Length=155, Percent_Identity=30.3225806451613, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325438, Length=153, Percent_Identity=30.0653594771242, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582879, Length=305, Percent_Identity=28.1967213114754, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28573937, Length=179, Percent_Identity=27.3743016759777, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24641311, Length=183, Percent_Identity=34.4262295081967, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24660640, Length=152, Percent_Identity=30.2631578947368, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 38046; Mature: 38046

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRYSRQMLFSSIGEDGQRKIREKHVLLIGAGALGAANAEALARAGIGKLTIADRDYVE
CCCHHHHHHHHHHHCCHHHHHHHHCEEEEEECCCCCCCCHHHHHHCCCCEEEECCCCCHH
WSNLQRQQLYTEEDAKQYKPKAIAAAEHLQKINSEVEIIPVVTDVAIQEMEDFIQGIDLI
HHCCHHHHHHCHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCEEE
IDATDNFDTRLLINDISQKYNVPWIYGGCVGSYGVTYTILPGKTPCFRCLMEHPTSGATC
EECCCCCCCEEHHHHHHHHCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCC
DTAGVIQPAVQMVVAHQVTEALKILVEDFGALRETMLSFDVWNNQHMAFKVNRQKKESCP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCC
SCGKLRTYPSLAFESQMKTEVLCGRNTVQIRPGVSKSLNLEEIKKRLQKNMDIQVTPYLL
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEHHHHH
SFFIEEYRFVLFKDGRAFVHGTNDKAEAKRLYTRYIG
HHHHHHHEEEEEECCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQDRYSRQMLFSSIGEDGQRKIREKHVLLIGAGALGAANAEALARAGIGKLTIADRDYVE
CCCHHHHHHHHHHHCCHHHHHHHHCEEEEEECCCCCCCCHHHHHHCCCCEEEECCCCCHH
WSNLQRQQLYTEEDAKQYKPKAIAAAEHLQKINSEVEIIPVVTDVAIQEMEDFIQGIDLI
HHCCHHHHHHCHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCEEE
IDATDNFDTRLLINDISQKYNVPWIYGGCVGSYGVTYTILPGKTPCFRCLMEHPTSGATC
EECCCCCCCEEHHHHHHHHCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCC
DTAGVIQPAVQMVVAHQVTEALKILVEDFGALRETMLSFDVWNNQHMAFKVNRQKKESCP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCC
SCGKLRTYPSLAFESQMKTEVLCGRNTVQIRPGVSKSLNLEEIKKRLQKNMDIQVTPYLL
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEHHHHH
SFFIEEYRFVLFKDGRAFVHGTNDKAEAKRLYTRYIG
HHHHHHHEEEEEECCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]