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The map label for this gene is iscS2 [H]

Identifier: 152977015

GI number: 152977015

Start: 3390062

End: 3391204

Strand: Reverse

Name: iscS2 [H]

Synonym: Bcer98_3320

Alternate gene names: 152977015

Gene position: 3391204-3390062 (Counterclockwise)

Preceding gene: 152977016

Following gene: 152977014

Centisome position: 82.97

GC content: 38.32

Gene sequence:

>1143_bases
ATGATCTATTTTGACAATAGTGCGACGACAAAGCCATATCCAGAAGTCCTACAATCTTATGTAACAGTTGCAGGGAAATA
CTTTGGAAATCCTTCTTCTATTCATTCGCTTGGAGGAGAAGCTGAACATTTATTAACGCAATCAAGAACGCTTGTAGCAC
AGCTTCTGCATGTTAAGCCTTCAGAAATTATTTTCACATCGGGTGGAACGGAAGGAAATAACCTTGCGATTAAAGGAATA
GCGATGAAGAATCGCTCTCGTGGCAAACATATTATTACAACAAATATTGAACACGCATCTGTGTTTGAAGCATATAAACA
ATTAGAGGAGCTTGGTTTTGAGGTAACATACTTACCTGTGAACGAGCATGGTGTTGTGTCTGTTCAAGATGTAAAACAAG
CAATTCGTGATGAAACCATTTTAGTTTCTATGATTCACGTCAATAATGAAACGGGGGCAATTCAGCCGATTACTGAGGTC
GGTGAATTGTTAACAAACTATCCGAAAATAAGATTTCATGTGGATCATGTACAAGGGATTGGAAAAGTACCGCTTGATTT
TCATGCTTCTCATATTGATCTTTGTTCAATATCAGGACATAAATTTCATAGTGTAAAAGGAACAGGACTTTTATATGTAC
GTGATGGCGTTCGGTTAGACCCGCTTTTATCAGGTGGCCAGCAAGAGCTTCGCTATCGTTCAGGTACAGAGAACTTACCT
GGTATTGTAGCTATGGTGAAGGCGCTGCGGATGACAATGGAACACATGAACGAAAAAATTACTCATTTACAAACGTTACA
AGCAGAATTGATTCGTTTCTTTGAGAGCATGGAAGGTGTAACAATTAATACATCACTTCAGCATGCTGCCCCTCATATTT
TAAATGTATCATTTGTTGGGTTAAAACCAGAGGTAGTTGTTCATGCTTTAGAGGAACATGAAGTATATGTATCAACGAAA
TCAGCTTGTTCTTCAAAAGCAAATGAGGTGAGCCGTGTATTAGTCTCAATGGGATTGCCATATGACGTAGCTGCGAGTGC
GATCCGCATTAGTTTAGCACCGGAAAATACAATGGAAGAAGTAAAACAATTTGAAGACATTATAAAAGAGACATTGCCAA
AATTATATGAAGTGATGAGGTAA

Upstream 100 bases:

>100_bases
TCGTTTCAAGATACAAAGCAAACCTTTGTATATGTTTAGCGTAATTGCACAGATTTATGATATGATTATATGATGTGACA
GCGCTGAAAGGGGAAGAACG

Downstream 100 bases:

>100_bases
AGAGAATATGATGAAATATGACCACATTTTAGTTCGTTATGGTGAAATGACGACAAAAGGAAAGAACCGTTCGAAGTTCG
TTAGTACGTTAAAAGATAAC

Product: aminotransferase class V

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIYFDNSATTKPYPEVLQSYVTVAGKYFGNPSSIHSLGGEAEHLLTQSRTLVAQLLHVKPSEIIFTSGGTEGNNLAIKGI
AMKNRSRGKHIITTNIEHASVFEAYKQLEELGFEVTYLPVNEHGVVSVQDVKQAIRDETILVSMIHVNNETGAIQPITEV
GELLTNYPKIRFHVDHVQGIGKVPLDFHASHIDLCSISGHKFHSVKGTGLLYVRDGVRLDPLLSGGQQELRYRSGTENLP
GIVAMVKALRMTMEHMNEKITHLQTLQAELIRFFESMEGVTINTSLQHAAPHILNVSFVGLKPEVVVHALEEHEVYVSTK
SACSSKANEVSRVLVSMGLPYDVAASAIRISLAPENTMEEVKQFEDIIKETLPKLYEVMR

Sequences:

>Translated_380_residues
MIYFDNSATTKPYPEVLQSYVTVAGKYFGNPSSIHSLGGEAEHLLTQSRTLVAQLLHVKPSEIIFTSGGTEGNNLAIKGI
AMKNRSRGKHIITTNIEHASVFEAYKQLEELGFEVTYLPVNEHGVVSVQDVKQAIRDETILVSMIHVNNETGAIQPITEV
GELLTNYPKIRFHVDHVQGIGKVPLDFHASHIDLCSISGHKFHSVKGTGLLYVRDGVRLDPLLSGGQQELRYRSGTENLP
GIVAMVKALRMTMEHMNEKITHLQTLQAELIRFFESMEGVTINTSLQHAAPHILNVSFVGLKPEVVVHALEEHEVYVSTK
SACSSKANEVSRVLVSMGLPYDVAASAIRISLAPENTMEEVKQFEDIIKETLPKLYEVMR
>Mature_380_residues
MIYFDNSATTKPYPEVLQSYVTVAGKYFGNPSSIHSLGGEAEHLLTQSRTLVAQLLHVKPSEIIFTSGGTEGNNLAIKGI
AMKNRSRGKHIITTNIEHASVFEAYKQLEELGFEVTYLPVNEHGVVSVQDVKQAIRDETILVSMIHVNNETGAIQPITEV
GELLTNYPKIRFHVDHVQGIGKVPLDFHASHIDLCSISGHKFHSVKGTGLLYVRDGVRLDPLLSGGQQELRYRSGTENLP
GIVAMVKALRMTMEHMNEKITHLQTLQAELIRFFESMEGVTINTSLQHAAPHILNVSFVGLKPEVVVHALEEHEVYVSTK
SACSSKANEVSRVLVSMGLPYDVAASAIRISLAPENTMEEVKQFEDIIKETLPKLYEVMR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=364, Percent_Identity=41.2087912087912, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=31.2348668280872, Blast_Score=181, Evalue=8e-46,
Organism=Escherichia coli, GI48994898, Length=383, Percent_Identity=40.9921671018277, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1787970, Length=269, Percent_Identity=24.5353159851301, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789175, Length=402, Percent_Identity=23.134328358209, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=390, Percent_Identity=40.5128205128205, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=33.2317073170732, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319831, Length=388, Percent_Identity=39.4329896907216, Blast_Score=255, Evalue=9e-69,
Organism=Drosophila melanogaster, GI20129463, Length=381, Percent_Identity=39.3700787401575, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42103; Mature: 42103

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNSATTKPYPEVLQSYVTVAGKYFGNPSSIHSLGGEAEHLLTQSRTLVAQLLHVKP
CEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
SEIIFTSGGTEGNNLAIKGIAMKNRSRGKHIITTNIEHASVFEAYKQLEELGFEVTYLPV
CCEEEECCCCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEE
NEHGVVSVQDVKQAIRDETILVSMIHVNNETGAIQPITEVGELLTNYPKIRFHVDHVQGI
CCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEHHHCCC
GKVPLDFHASHIDLCSISGHKFHSVKGTGLLYVRDGVRLDPLLSGGQQELRYRSGTENLP
CCCCCCCCCCEEEEEECCCCEEEEECCCEEEEEECCCEECHHHCCCHHHHHHCCCCCCCC
GIVAMVKALRMTMEHMNEKITHLQTLQAELIRFFESMEGVTINTSLQHAAPHILNVSFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCEEEEEEEC
LKPEVVVHALEEHEVYVSTKSACSSKANEVSRVLVSMGLPYDVAASAIRISLAPENTMEE
CCHHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCHHHHH
VKQFEDIIKETLPKLYEVMR
HHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIYFDNSATTKPYPEVLQSYVTVAGKYFGNPSSIHSLGGEAEHLLTQSRTLVAQLLHVKP
CEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
SEIIFTSGGTEGNNLAIKGIAMKNRSRGKHIITTNIEHASVFEAYKQLEELGFEVTYLPV
CCEEEECCCCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEE
NEHGVVSVQDVKQAIRDETILVSMIHVNNETGAIQPITEVGELLTNYPKIRFHVDHVQGI
CCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEHHHCCC
GKVPLDFHASHIDLCSISGHKFHSVKGTGLLYVRDGVRLDPLLSGGQQELRYRSGTENLP
CCCCCCCCCCEEEEEECCCCEEEEECCCEEEEEECCCEECHHHCCCHHHHHHCCCCCCCC
GIVAMVKALRMTMEHMNEKITHLQTLQAELIRFFESMEGVTINTSLQHAAPHILNVSFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCEEEEEEEC
LKPEVVVHALEEHEVYVSTKSACSSKANEVSRVLVSMGLPYDVAASAIRISLAPENTMEE
CCHHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCHHHHH
VKQFEDIIKETLPKLYEVMR
HHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]