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The map label for this gene is ytsJ [H]

Identifier: 152976984

GI number: 152976984

Start: 3357255

End: 3358493

Strand: Reverse

Name: ytsJ [H]

Synonym: Bcer98_3287

Alternate gene names: 152976984

Gene position: 3358493-3357255 (Counterclockwise)

Preceding gene: 152976985

Following gene: 152976983

Centisome position: 82.17

GC content: 40.84

Gene sequence:

>1239_bases
TTGTCAACACTTCGTGAAGAAGCACTTCACATGCATAAAGTGCATCAAGGAAAATTAGAAACTGTATCAAAAGTGAAAGT
AGAAAATGCAAAAGACTTAAGTCTTGCATATTCTCCAGGGGTTGCAGAACCTTGTAAAGAAATTTATGACGATAAAAGTA
AGGTATATGAATATACAATGAAGGGAAACATGGTAGCTGTTGTAACAGATGGGACAGCAGTACTTGGTCTTGGGAATATT
GGACCAGAAGCATCTCTACCTGTAATGGAAGGTAAAGCGGTATTATTTAAGAGCTTTGCTGGCGTAGATGCATTCCCGAT
TGCTTTAAATACAAACGATGTAGAAAAAATTATTGAAACTGTAAAATTAATGGAGCCAAACTTTGGCGGTGTAAACTTAG
AAGATATTGCAGCTCCAAACTGTTTTATTATTGAAGAACGCTTGAAAAAAGAAACGAATATTCCTGTATTCCATGATGAT
CAACACGGAACAGCTATCGTAACAGTGGCAGGTCTTGTAAATGCTCTGAAGTTAGTTGGAAAGAAAATGTCTGATATTAA
AGTCGTGGCAAATGGTGCAGGTGCAGCGGGTATTGCGATTATTAAACTTTTATACCGCTACGGTGTGCGCGACATTATTA
TGTGTGACCGTAAAGGAGCGATTTACGACGGCCGCCCTGTTGGTATGAATCCAGTGAAAGAAGAAGTTGCAAAATATACA
AATAAAGACCGTGTAGAAGGTTCTTTAACGGATGTTATAAAAGGTGCGGATGTATTCATCGGTGTATCTGTAGAAGGTGC
ATTAACGGAAGAAATGGTTCGTACGATGAATAACGATGCGATTATCTTTGCAATGGCAAATCCGGTTCCAGAAATCATGC
CAGAAGCAGCAAAAGCAGCGGGTGCAGCGGTTGTTGGAACGGGTCGTTCTGACTTCCCGAACCAAGTAAATAATGTGTTA
GCATTCCCTGGCATTTTCCGTGGTGCACTTGATGTACATGCTACCCAAATTAATGAAGAAATGAAAATGGCAGCTGTAGC
GGCTATAGCAGAGCTTGTAACAGCTGATGAATTAAATGAAGACTATATCATTCCGGCGCCATTTGACGCGCGTGTAGCGC
CTCAGGTAGCGGCTTATGTAGCGAAAGCTGCAATGGAGACAGGGGTAGCTCGTCGCCAAGTAGATCCAAATGAAGTTGCT
GAGAAAACAAAACGATTAGCGTTGATTGGTAACGAATAA

Upstream 100 bases:

>100_bases
ACAACGCCCCTATAGTTCGATTATTATAGTAGTAGTGTTCGTGGTCAGACCACTCGGAAAAATTGATAGCAGCAAGAATA
ATTTGAGGAGAGTGGATAGT

Downstream 100 bases:

>100_bases
GCGAGGAGTTTTAATTGACATCATCAAATACAAAAGTATACCTCGAAATTGTAAAGAAAATTCGCTCCATTATGGAGGAG
GATGGTTTAGTTGCAGGTGA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSTLREEALHMHKVHQGKLETVSKVKVENAKDLSLAYSPGVAEPCKEIYDDKSKVYEYTMKGNMVAVVTDGTAVLGLGNI
GPEASLPVMEGKAVLFKSFAGVDAFPIALNTNDVEKIIETVKLMEPNFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDD
QHGTAIVTVAGLVNALKLVGKKMSDIKVVANGAGAAGIAIIKLLYRYGVRDIIMCDRKGAIYDGRPVGMNPVKEEVAKYT
NKDRVEGSLTDVIKGADVFIGVSVEGALTEEMVRTMNNDAIIFAMANPVPEIMPEAAKAAGAAVVGTGRSDFPNQVNNVL
AFPGIFRGALDVHATQINEEMKMAAVAAIAELVTADELNEDYIIPAPFDARVAPQVAAYVAKAAMETGVARRQVDPNEVA
EKTKRLALIGNE

Sequences:

>Translated_412_residues
MSTLREEALHMHKVHQGKLETVSKVKVENAKDLSLAYSPGVAEPCKEIYDDKSKVYEYTMKGNMVAVVTDGTAVLGLGNI
GPEASLPVMEGKAVLFKSFAGVDAFPIALNTNDVEKIIETVKLMEPNFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDD
QHGTAIVTVAGLVNALKLVGKKMSDIKVVANGAGAAGIAIIKLLYRYGVRDIIMCDRKGAIYDGRPVGMNPVKEEVAKYT
NKDRVEGSLTDVIKGADVFIGVSVEGALTEEMVRTMNNDAIIFAMANPVPEIMPEAAKAAGAAVVGTGRSDFPNQVNNVL
AFPGIFRGALDVHATQINEEMKMAAVAAIAELVTADELNEDYIIPAPFDARVAPQVAAYVAKAAMETGVARRQVDPNEVA
EKTKRLALIGNE
>Mature_411_residues
STLREEALHMHKVHQGKLETVSKVKVENAKDLSLAYSPGVAEPCKEIYDDKSKVYEYTMKGNMVAVVTDGTAVLGLGNIG
PEASLPVMEGKAVLFKSFAGVDAFPIALNTNDVEKIIETVKLMEPNFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQ
HGTAIVTVAGLVNALKLVGKKMSDIKVVANGAGAAGIAIIKLLYRYGVRDIIMCDRKGAIYDGRPVGMNPVKEEVAKYTN
KDRVEGSLTDVIKGADVFIGVSVEGALTEEMVRTMNNDAIIFAMANPVPEIMPEAAKAAGAAVVGTGRSDFPNQVNNVLA
FPGIFRGALDVHATQINEEMKMAAVAAIAELVTADELNEDYIIPAPFDARVAPQVAAYVAKAAMETGVARRQVDPNEVAE
KTKRLALIGNE

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=396, Percent_Identity=28.7878787878788, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI62420880, Length=396, Percent_Identity=28.7878787878788, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI239049447, Length=396, Percent_Identity=28.7878787878788, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4505143, Length=394, Percent_Identity=25.1269035532995, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI4505145, Length=406, Percent_Identity=25.3694581280788, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI270265879, Length=320, Percent_Identity=26.875, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788806, Length=409, Percent_Identity=52.3227383863081, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI87081919, Length=458, Percent_Identity=28.3842794759825, Blast_Score=108, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17537199, Length=452, Percent_Identity=26.3274336283186, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6322823, Length=311, Percent_Identity=30.5466237942122, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356279, Length=469, Percent_Identity=26.0127931769723, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI281362672, Length=469, Percent_Identity=26.0127931769723, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI281362674, Length=469, Percent_Identity=26.0127931769723, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI78707242, Length=408, Percent_Identity=26.9607843137255, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI78707238, Length=408, Percent_Identity=26.9607843137255, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24646386, Length=444, Percent_Identity=25.6756756756757, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24646388, Length=393, Percent_Identity=26.972010178117, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI78707240, Length=397, Percent_Identity=26.9521410579345, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 44138; Mature: 44007

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLREEALHMHKVHQGKLETVSKVKVENAKDLSLAYSPGVAEPCKEIYDDKSKVYEYTM
CCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHCCHHHHEEEEE
KGNMVAVVTDGTAVLGLGNIGPEASLPVMEGKAVLFKSFAGVDAFPIALNTNDVEKIIET
CCCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHH
VKLMEPNFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLVG
HHHCCCCCCCCCHHHHCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHHC
KKMSDIKVVANGAGAAGIAIIKLLYRYGVRDIIMCDRKGAIYDGRPVGMNPVKEEVAKYT
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCHHHHHHHHHC
NKDRVEGSLTDVIKGADVFIGVSVEGALTEEMVRTMNNDAIIFAMANPVPEIMPEAAKAA
CCHHCCCHHHHHHCCCCEEEEEEECHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHC
GAAVVGTGRSDFPNQVNNVLAFPGIFRGALDVHATQINEEMKMAAVAAIAELVTADELNE
CCEEEECCCCCCCHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DYIIPAPFDARVAPQVAAYVAKAAMETGVARRQVDPNEVAEKTKRLALIGNE
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCC
>Mature Secondary Structure 
STLREEALHMHKVHQGKLETVSKVKVENAKDLSLAYSPGVAEPCKEIYDDKSKVYEYTM
CCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHCCHHHHEEEEE
KGNMVAVVTDGTAVLGLGNIGPEASLPVMEGKAVLFKSFAGVDAFPIALNTNDVEKIIET
CCCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHH
VKLMEPNFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLVG
HHHCCCCCCCCCHHHHCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHHC
KKMSDIKVVANGAGAAGIAIIKLLYRYGVRDIIMCDRKGAIYDGRPVGMNPVKEEVAKYT
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCHHHHHHHHHC
NKDRVEGSLTDVIKGADVFIGVSVEGALTEEMVRTMNNDAIIFAMANPVPEIMPEAAKAA
CCHHCCCHHHHHHCCCCEEEEEEECHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHC
GAAVVGTGRSDFPNQVNNVLAFPGIFRGALDVHATQINEEMKMAAVAAIAELVTADELNE
CCEEEECCCCCCCHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DYIIPAPFDARVAPQVAAYVAKAAMETGVARRQVDPNEVAEKTKRLALIGNE
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]