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The map label for this gene is dgkA [H]

Identifier: 152976732

GI number: 152976732

Start: 3074876

End: 3075229

Strand: Reverse

Name: dgkA [H]

Synonym: Bcer98_3028

Alternate gene names: 152976732

Gene position: 3075229-3074876 (Counterclockwise)

Preceding gene: 152976733

Following gene: 152976731

Centisome position: 75.24

GC content: 33.9

Gene sequence:

>354_bases
ATGAAAAAAGGAAAACTTATAAAGAGTTTTACGTATGCTATAGCTGGTATATGTTTTTGTCTTCGTCATGAACGAAATAT
GAAAATTCATTGTTTGGCCGCAGTTATTGTTATATGCTGCGGCCTTTATTTCCATGTAACAAGAGTAGAATGGCTCATAT
TACTTGTTGTAATAGGGGCTGTAATGAGTTTGGAGATGGTAAATACAGCGATTGAAAAAACGGTTGATTTAGTAACAGAG
GATTTTAAACCCCTTGCAAAAATTGCAAAAGATGTTGCAGCTGGTGCCGTTTTATTATTTACTATTATAGCGGTCATAAT
TGGTGCTATTATCTTTTTACCTTATGTGGTATAG

Upstream 100 bases:

>100_bases
GATTTTTACACTTGCTTGGTTATGATCATATGACTGAAGAAGATGAAAAAGAGATGTTTGGAAAGCAAAAAGAAATTTTA
GGGGCATTTGGATTAAGAAG

Downstream 100 bases:

>100_bases
TTGCATTCCAAAGATTTTCTTGTACTTGAGTGGTATTGAGTTTTTCATCAGTTGATGATTTATTCAGGTGGCCATAAAAG
GGATAAGGAATATGGATCCT

Product: diacylglycerol kinase

Products: NA

Alternate protein names: UdpK [H]

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MKKGKLIKSFTYAIAGICFCLRHERNMKIHCLAAVIVICCGLYFHVTRVEWLILLVVIGAVMSLEMVNTAIEKTVDLVTE
DFKPLAKIAKDVAAGAVLLFTIIAVIIGAIIFLPYVV

Sequences:

>Translated_117_residues
MKKGKLIKSFTYAIAGICFCLRHERNMKIHCLAAVIVICCGLYFHVTRVEWLILLVVIGAVMSLEMVNTAIEKTVDLVTE
DFKPLAKIAKDVAAGAVLLFTIIAVIIGAIIFLPYVV
>Mature_117_residues
MKKGKLIKSFTYAIAGICFCLRHERNMKIHCLAAVIVICCGLYFHVTRVEWLILLVVIGAVMSLEMVNTAIEKTVDLVTE
DFKPLAKIAKDVAAGAVLLFTIIAVIIGAIIFLPYVV

Specific function: Catalyzes the phosphorylation of undecaprenol, which is probably the primary physiological substrate. Is also able to phosphorylate diacylglycerol, albeit with very low efficiency. May play a role in adaptability to environmental stress conditions such as

COG id: COG0818

COG function: function code M; Diacylglycerol kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial diacylglycerol kinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000829 [H]

Pfam domain/function: PF01219 DAGK_prokar [H]

EC number: =2.7.1.66 [H]

Molecular weight: Translated: 12880; Mature: 12880

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS01069 DAGK_PROKAR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGKLIKSFTYAIAGICFCLRHERNMKIHCLAAVIVICCGLYFHVTRVEWLILLVVIGA
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMSLEMVNTAIEKTVDLVTEDFKPLAKIAKDVAAGAVLLFTIIAVIIGAIIFLPYVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKGKLIKSFTYAIAGICFCLRHERNMKIHCLAAVIVICCGLYFHVTRVEWLILLVVIGA
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMSLEMVNTAIEKTVDLVTEDFKPLAKIAKDVAAGAVLLFTIIAVIIGAIIFLPYVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8407794; 9422608; 12397186 [H]