Click here to switch to the map view.

The map label for this gene is bfmBC [H]

Identifier: 152976568

GI number: 152976568

Start: 2929401

End: 2930822

Strand: Reverse

Name: bfmBC [H]

Synonym: Bcer98_2857

Alternate gene names: 152976568

Gene position: 2930822-2929401 (Counterclockwise)

Preceding gene: 152976569

Following gene: 152976567

Centisome position: 71.71

GC content: 39.17

Gene sequence:

>1422_bases
ATGGCAAAAGAATATGATTTAGTCATCGTTGGTGGCGGAACAGGTGGATATGTTGCTGCTATTCGTGCATCGCAGTTAGG
ACTAAAAACAGCTCTTGTTGAAAAGGATAATCTTGGTGGCACGTGCTTGCATAAAGGGTGTATTCCAAGCAAAGCTTTAC
TCCGTAGTGCGGAAGTATATGCAACCGCTAAAAAGGGAGAAGAGTTTGGTGTTGTGACAAGTAATGTAGAGTTAAATTTT
GCGAAAGTACAAGAGCGTAAGGGAAAAATTGTAGCGCAACTTCATAAAGGGGTTCAGCACTTAATGAAGCAAGGGAAAAT
TGATGTATTTGAAGGGATTGGTCGTATACTAGGTCCATCAATTTTCTCACCGATGCCAGGAACAATTTCGGTTGAATTTA
CAAATGGTGAAGAGAATGAAATGTTAATTCCGAAAAATGTAATTATTGCAACTGGTTCCCGTCCAAACTCGTTACCTGGA
TTAGAATTAGACGGAGAATACGTTATGTCCTCGGATCACGCCTTGAAAATGGAAACTCTTCCTAAGTCTATTATTATTGT
TGGCGGTGGCGTAATTGGAATCGAATGGGCATCTATGCTTGCTGATTTTGGCGTAGAAGTGACGATATTAGAATATGCAA
AACATGTTCTTCCACTTGAGGATCAAGATGTGTCAAAAGAAATGCAACGTCTCTTAAAGAAAAAAGGAATTAAGGTGGTG
ACGGGTGCAAAAGTATTACCAGAAACATTAGGAAAAGATAATGGAGTAACTATACAGGCAGAACATAATGGTGAAAAGAA
AGAATTTCAAGCAGAAAAAATGCTTGTATCTGTGGGAAGACAAGCCAATACACAAAATATCGGATTGGAAAATACAGATA
TCGTTGTGGAAAAAGGATACATTCAAACAAATGAATTTTATCAAACGAAAGAATCTCACATTTATGCAATTGGTGATGTA
ATCGGAGGATTACAACTTGCACATGTGGCTTCACATGAAGGGATCGTTGCCGTAGAGCATATTGCTGGAAAAGATGTTAT
GCCAATTGATTACTCTATGGTATCAAAATGTGTTTATAGCAATCCGGAAGTTGCATCTGTTGGTTTAACAGAACAAGAGG
CAAAAGAAAAAGGCTATAAGCTAAAGGTAGGTAAGTTCTCATTCCGTGCAATCGGAAAGGCACTTGTATATGGAGAGTCA
GATGGGTTTGTGAAACTTGTGGTTGATGAAGAAACCAATGATATTCTTGGTGTCCATATGATTGGTCCACATGTAACAGA
TATGATTTCTGAAGCTGGCCTTGCAAGAGTGTTGGATGCAACACCTTGGGAAGTGGCACATACAATTCATCCGCATCCAT
CATTATCAGAAGCGATTGGTGAAGCAGCACTAGCTGTAGATGGAAAAGCGTTACACGCATAA

Upstream 100 bases:

>100_bases
CAAGCATTAGCGGAAGGAGCCCTTCGTGTATTACGGGAAGAAGAAGCTCCAAAAGAGTATGTTGTACGAGAAAAAGAAAC
GGTAGCTAGGGGTTGAGAGA

Downstream 100 bases:

>100_bases
AAATGTGGATTTAGGAGGTTATGAAAAATGGCAGAAGTAAAAGAAAAACGCCATGAAGCGCTTGGCTTAAGTGATGAGCA
AGTATTAGAAATGTACCGTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKDNLGGTCLHKGCIPSKALLRSAEVYATAKKGEEFGVVTSNVELNF
AKVQERKGKIVAQLHKGVQHLMKQGKIDVFEGIGRILGPSIFSPMPGTISVEFTNGEENEMLIPKNVIIATGSRPNSLPG
LELDGEYVMSSDHALKMETLPKSIIIVGGGVIGIEWASMLADFGVEVTILEYAKHVLPLEDQDVSKEMQRLLKKKGIKVV
TGAKVLPETLGKDNGVTIQAEHNGEKKEFQAEKMLVSVGRQANTQNIGLENTDIVVEKGYIQTNEFYQTKESHIYAIGDV
IGGLQLAHVASHEGIVAVEHIAGKDVMPIDYSMVSKCVYSNPEVASVGLTEQEAKEKGYKLKVGKFSFRAIGKALVYGES
DGFVKLVVDEETNDILGVHMIGPHVTDMISEAGLARVLDATPWEVAHTIHPHPSLSEAIGEAALAVDGKALHA

Sequences:

>Translated_473_residues
MAKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKDNLGGTCLHKGCIPSKALLRSAEVYATAKKGEEFGVVTSNVELNF
AKVQERKGKIVAQLHKGVQHLMKQGKIDVFEGIGRILGPSIFSPMPGTISVEFTNGEENEMLIPKNVIIATGSRPNSLPG
LELDGEYVMSSDHALKMETLPKSIIIVGGGVIGIEWASMLADFGVEVTILEYAKHVLPLEDQDVSKEMQRLLKKKGIKVV
TGAKVLPETLGKDNGVTIQAEHNGEKKEFQAEKMLVSVGRQANTQNIGLENTDIVVEKGYIQTNEFYQTKESHIYAIGDV
IGGLQLAHVASHEGIVAVEHIAGKDVMPIDYSMVSKCVYSNPEVASVGLTEQEAKEKGYKLKVGKFSFRAIGKALVYGES
DGFVKLVVDEETNDILGVHMIGPHVTDMISEAGLARVLDATPWEVAHTIHPHPSLSEAIGEAALAVDGKALHA
>Mature_472_residues
AKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKDNLGGTCLHKGCIPSKALLRSAEVYATAKKGEEFGVVTSNVELNFA
KVQERKGKIVAQLHKGVQHLMKQGKIDVFEGIGRILGPSIFSPMPGTISVEFTNGEENEMLIPKNVIIATGSRPNSLPGL
ELDGEYVMSSDHALKMETLPKSIIIVGGGVIGIEWASMLADFGVEVTILEYAKHVLPLEDQDVSKEMQRLLKKKGIKVVT
GAKVLPETLGKDNGVTIQAEHNGEKKEFQAEKMLVSVGRQANTQNIGLENTDIVVEKGYIQTNEFYQTKESHIYAIGDVI
GGLQLAHVASHEGIVAVEHIAGKDVMPIDYSMVSKCVYSNPEVASVGLTEQEAKEKGYKLKVGKFSFRAIGKALVYGESD
GFVKLVVDEETNDILGVHMIGPHVTDMISEAGLARVLDATPWEVAHTIHPHPSLSEAIGEAALAVDGKALHA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=479, Percent_Identity=40.5010438413361, Blast_Score=323, Evalue=1e-88,
Organism=Homo sapiens, GI50301238, Length=471, Percent_Identity=27.8131634819533, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=445, Percent_Identity=24.9438202247191, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519428, Length=445, Percent_Identity=24.9438202247191, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519426, Length=445, Percent_Identity=24.9438202247191, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI148277065, Length=445, Percent_Identity=24.9438202247191, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI148277071, Length=445, Percent_Identity=24.9438202247191, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI22035672, Length=485, Percent_Identity=26.3917525773196, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI291045266, Length=438, Percent_Identity=24.2009132420091, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI291045268, Length=435, Percent_Identity=22.0689655172414, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786307, Length=465, Percent_Identity=35.2688172043011, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=27.0386266094421, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=29.2735042735043, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1789915, Length=466, Percent_Identity=28.9699570815451, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=478, Percent_Identity=42.4686192468619, Blast_Score=343, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17557007, Length=481, Percent_Identity=25.7796257796258, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=463, Percent_Identity=25.9179265658747, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=463, Percent_Identity=25.9179265658747, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=492, Percent_Identity=25.2032520325203, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=483, Percent_Identity=38.3022774327122, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6325240, Length=482, Percent_Identity=28.4232365145228, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325166, Length=473, Percent_Identity=26.4270613107822, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=42.0940170940171, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=24.6406570841889, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640549, Length=487, Percent_Identity=24.6406570841889, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=24.6406570841889, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=22.3628691983122, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50794; Mature: 50663

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKDNLGGTCLHKGCIPSKALLRSAEVY
CCCEEEEEEEECCCCCEEEEEEHHHCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHHHE
ATAKKGEEFGVVTSNVELNFAKVQERKGKIVAQLHKGVQHLMKQGKIDVFEGIGRILGPS
EEECCCCCCCEEEECEEEEHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHH
IFSPMPGTISVEFTNGEENEMLIPKNVIIATGSRPNSLPGLELDGEYVMSSDHALKMETL
HCCCCCCEEEEEEECCCCCCEECCCCEEEEECCCCCCCCCEEECCCEEECCCCEEEEECC
PKSIIIVGGGVIGIEWASMLADFGVEVTILEYAKHVLPLEDQDVSKEMQRLLKKKGIKVV
CCEEEEEECCEEEHHHHHHHHHCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCEEE
TGAKVLPETLGKDNGVTIQAEHNGEKKEFQAEKMLVSVGRQANTQNIGLENTDIVVEKGY
ECCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCE
IQTNEFYQTKESHIYAIGDVIGGLQLAHVASHEGIVAVEHIAGKDVMPIDYSMVSKCVYS
EECCHHHCCCCCEEEEEHHHHCCHHEEEHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
NPEVASVGLTEQEAKEKGYKLKVGKFSFRAIGKALVYGESDGFVKLVVDEETNDILGVHM
CCCEEEECCCHHHHHHCCCEEEECCCHHHHHCEEEEEECCCCEEEEEEECCCCCEEEEEE
IGPHVTDMISEAGLARVLDATPWEVAHTIHPHPSLSEAIGEAALAVDGKALHA
ECCHHHHHHHHCCHHHHHCCCCHHHEEECCCCCCHHHHHCCCEEEECCHHCCC
>Mature Secondary Structure 
AKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKDNLGGTCLHKGCIPSKALLRSAEVY
CCEEEEEEEECCCCCEEEEEEHHHCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHHHE
ATAKKGEEFGVVTSNVELNFAKVQERKGKIVAQLHKGVQHLMKQGKIDVFEGIGRILGPS
EEECCCCCCCEEEECEEEEHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHH
IFSPMPGTISVEFTNGEENEMLIPKNVIIATGSRPNSLPGLELDGEYVMSSDHALKMETL
HCCCCCCEEEEEEECCCCCCEECCCCEEEEECCCCCCCCCEEECCCEEECCCCEEEEECC
PKSIIIVGGGVIGIEWASMLADFGVEVTILEYAKHVLPLEDQDVSKEMQRLLKKKGIKVV
CCEEEEEECCEEEHHHHHHHHHCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCEEE
TGAKVLPETLGKDNGVTIQAEHNGEKKEFQAEKMLVSVGRQANTQNIGLENTDIVVEKGY
ECCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCE
IQTNEFYQTKESHIYAIGDVIGGLQLAHVASHEGIVAVEHIAGKDVMPIDYSMVSKCVYS
EECCHHHCCCCCEEEEEHHHHCCHHEEEHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
NPEVASVGLTEQEAKEKGYKLKVGKFSFRAIGKALVYGESDGFVKLVVDEETNDILGVHM
CCCEEEECCCHHHHHHCCCEEEECCCHHHHHCEEEEEECCCCEEEEEEECCCCCEEEEEE
IGPHVTDMISEAGLARVLDATPWEVAHTIHPHPSLSEAIGEAALAVDGKALHA
ECCHHHHHHHHCCHHHHHCCCCHHHEEECCCCCCHHHHHCCCEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]