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The map label for this gene is bfmBB [H]

Identifier: 152976565

GI number: 152976565

Start: 2926042

End: 2927358

Strand: Reverse

Name: bfmBB [H]

Synonym: Bcer98_2854

Alternate gene names: 152976565

Gene position: 2927358-2926042 (Counterclockwise)

Preceding gene: 152976566

Following gene: 152976564

Centisome position: 71.63

GC content: 39.26

Gene sequence:

>1317_bases
ATGGCTGTAGAAAATATTACAATGCCTCAGCTCGGGGAGAGCGTAACAGAAGGTACAATTAGCAAATGGCTTGTAAATGT
TGGGGATCATGTGAATAAATATGATCCAATCGCAGAAGTAATGACAGATAAGGTAAATGCAGAAGTACCATCTTCTTTCA
CCGGGGTTGTGAAAGAGTTAGTTGCAGCGGAAGGAGACACACTTGCTGTAGGGGAAGTTGTTTGTGTCATTCAAGTAGAG
GGAGCAGATGAAGTAGCAGCAACAGCTGTAGAAGAGAAAACGAAAGAGGAAGCAAAAGCAGAGGTTGTCTCAGCTGAAAA
AGTAGCAAAAACAAAGCAACCAACTGACGGGAAACCTCGTTATTCTCCAGCGGTATTAAAGCTTGCAGGTGAACATAATA
TCGATTTAAATGTAGTAGAAGGTACAGGAGCAAATGGACGCATTACACGTAAAGATATTTTGAAACTTGTGGAATCAGGA
AATATTCCGCAAGCGGATGCGAAAAAAGAGGAAGTAGCTGTATCAACACCTACTCCTCAAGAAGCGCCAAAAGCAGAAGA
GAAAGCGCCAGTACAAAAAACAGAAGCGAAACCAGTATCTGTACCAACCGTACCTGGTGATATTGAAATTCCAGTAACAG
GTGTGCGTAAAGCTATTGCTGCGAATATGTTACGCAGTAAACATGAGGCACCTCACGCTTGGATGATGATTGAAGTAGAT
GTGACAGATCTTGTATCGTATCGTAACGCAATTAAAAATGAATTTAAGAAACGTGAAGGCTTTAACTTAACATTCTTTGC
ATTCTTCGTAAAAGCAGTTGCACAGGCGTTAAAAGAATACCCGCAAATTAATTCTATGTGGGCTGGTGATAAAATCGTTC
AGAAGAAAGATATTAACCTTTCTATTGCTGTTGCAACAGAAGAGGAATTATTTGTTCCAGTGATTAAACATGCAGACGAA
AAAACAATTAAAGGTATTGCTCGTGAAATTACGGAGCTTGCAACGAAAGTTCGTACAAAATCATTAAAACCAGATGAAAT
GCAAGGCGGTACATTTACAATTAATAACACAGGATCATTTGGTTCTGTTCAATCTATGGGTATTATTAACCATCCACAAG
CTGCTATTTTACAAGTAGAATCAATTGTAAAACGTCCGGTTATTATGGATAACGGCATGTTTGGGGCACGTGACATGGTT
AATTTATGTTTATCGCTTGATCATCGCGTACTTGACGGTTTGATCTGTGGCAAGTTCTTAGGTCGAGTGAAAGAGATTTT
AGAAAATATGTCAGCGGAGAATACATCTGTATATTAA

Upstream 100 bases:

>100_bases
GCCATATGCACCAACAATGGAAAAATTCTTTATGGTAAATCCAGATAAAGTTGAAAAAGCAATGCGTGAACTTGCGGAAT
TTTAATCGGGGGGACTATAC

Downstream 100 bases:

>100_bases
TAAAAAGCTCGCGAAATGCGAGCTTTTTACATTTGTACAAAAGGTGATATCTTTCATTTCTATCTTAATATCTGACAATG
AGTCATAATGTAGAAAGAAT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPIAEVMTDKVNAEVPSSFTGVVKELVAAEGDTLAVGEVVCVIQVE
GADEVAATAVEEKTKEEAKAEVVSAEKVAKTKQPTDGKPRYSPAVLKLAGEHNIDLNVVEGTGANGRITRKDILKLVESG
NIPQADAKKEEVAVSTPTPQEAPKAEEKAPVQKTEAKPVSVPTVPGDIEIPVTGVRKAIAANMLRSKHEAPHAWMMIEVD
VTDLVSYRNAIKNEFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINLSIAVATEEELFVPVIKHADE
KTIKGIAREITELATKVRTKSLKPDEMQGGTFTINNTGSFGSVQSMGIINHPQAAILQVESIVKRPVIMDNGMFGARDMV
NLCLSLDHRVLDGLICGKFLGRVKEILENMSAENTSVY

Sequences:

>Translated_438_residues
MAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPIAEVMTDKVNAEVPSSFTGVVKELVAAEGDTLAVGEVVCVIQVE
GADEVAATAVEEKTKEEAKAEVVSAEKVAKTKQPTDGKPRYSPAVLKLAGEHNIDLNVVEGTGANGRITRKDILKLVESG
NIPQADAKKEEVAVSTPTPQEAPKAEEKAPVQKTEAKPVSVPTVPGDIEIPVTGVRKAIAANMLRSKHEAPHAWMMIEVD
VTDLVSYRNAIKNEFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINLSIAVATEEELFVPVIKHADE
KTIKGIAREITELATKVRTKSLKPDEMQGGTFTINNTGSFGSVQSMGIINHPQAAILQVESIVKRPVIMDNGMFGARDMV
NLCLSLDHRVLDGLICGKFLGRVKEILENMSAENTSVY
>Mature_437_residues
AVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPIAEVMTDKVNAEVPSSFTGVVKELVAAEGDTLAVGEVVCVIQVEG
ADEVAATAVEEKTKEEAKAEVVSAEKVAKTKQPTDGKPRYSPAVLKLAGEHNIDLNVVEGTGANGRITRKDILKLVESGN
IPQADAKKEEVAVSTPTPQEAPKAEEKAPVQKTEAKPVSVPTVPGDIEIPVTGVRKAIAANMLRSKHEAPHAWMMIEVDV
TDLVSYRNAIKNEFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINLSIAVATEEELFVPVIKHADEK
TIKGIAREITELATKVRTKSLKPDEMQGGTFTINNTGSFGSVQSMGIINHPQAAILQVESIVKRPVIMDNGMFGARDMVN
LCLSLDHRVLDGLICGKFLGRVKEILENMSAENTSVY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=448, Percent_Identity=30.8035714285714, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI203098816, Length=446, Percent_Identity=30.4932735426009, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI203098753, Length=446, Percent_Identity=30.4932735426009, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=31.4814814814815, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI19923748, Length=217, Percent_Identity=36.405529953917, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=37.5796178343949, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1786946, Length=429, Percent_Identity=31.7016317016317, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786305, Length=446, Percent_Identity=30.7174887892377, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=438, Percent_Identity=28.7671232876712, Blast_Score=184, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=435, Percent_Identity=28.9655172413793, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=454, Percent_Identity=28.1938325991189, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=30, Blast_Score=128, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=429, Percent_Identity=31.2354312354312, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=26.1538461538462, Blast_Score=135, Evalue=9e-33,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=30.9633027522936, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24582497, Length=225, Percent_Identity=33.7777777777778, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI20129315, Length=225, Percent_Identity=33.7777777777778, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24645909, Length=240, Percent_Identity=34.1666666666667, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 47540; Mature: 47409

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPIAEVMTDKVNAEVPSSFTGVVKEL
CCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
VAAEGDTLAVGEVVCVIQVEGADEVAATAVEEKTKEEAKAEVVSAEKVAKTKQPTDGKPR
HHCCCCEEEECEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YSPAVLKLAGEHNIDLNVVEGTGANGRITRKDILKLVESGNIPQADAKKEEVAVSTPTPQ
CCCHHEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCC
EAPKAEEKAPVQKTEAKPVSVPTVPGDIEIPVTGVRKAIAANMLRSKHEAPHAWMMIEVD
CCCCCHHCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
VTDLVSYRNAIKNEFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEECCCCE
SIAVATEEELFVPVIKHADEKTIKGIAREITELATKVRTKSLKPDEMQGGTFTINNTGSF
EEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
GSVQSMGIINHPQAAILQVESIVKRPVIMDNGMFGARDMVNLCLSLDHRVLDGLICGKFL
CCHHCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GRVKEILENMSAENTSVY
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPIAEVMTDKVNAEVPSSFTGVVKEL
CCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
VAAEGDTLAVGEVVCVIQVEGADEVAATAVEEKTKEEAKAEVVSAEKVAKTKQPTDGKPR
HHCCCCEEEECEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YSPAVLKLAGEHNIDLNVVEGTGANGRITRKDILKLVESGNIPQADAKKEEVAVSTPTPQ
CCCHHEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCC
EAPKAEEKAPVQKTEAKPVSVPTVPGDIEIPVTGVRKAIAANMLRSKHEAPHAWMMIEVD
CCCCCHHCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
VTDLVSYRNAIKNEFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEECCCCE
SIAVATEEELFVPVIKHADEKTIKGIAREITELATKVRTKSLKPDEMQGGTFTINNTGSF
EEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
GSVQSMGIINHPQAAILQVESIVKRPVIMDNGMFGARDMVNLCLSLDHRVLDGLICGKFL
CCHHCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GRVKEILENMSAENTSVY
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]