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The map label for this gene is bioB

Identifier: 152976524

GI number: 152976524

Start: 2888490

End: 2889488

Strand: Reverse

Name: bioB

Synonym: Bcer98_2813

Alternate gene names: 152976524

Gene position: 2889488-2888490 (Counterclockwise)

Preceding gene: 152976525

Following gene: 152976523

Centisome position: 70.7

GC content: 38.14

Gene sequence:

>999_bases
ATGAAACAAATACAAACAAAAGTAGATTGGAAGAAAATTGCGTTTGAAGGGATAGAAGGAAAAAGGATAACGAAAGAGGA
TGCGTTAGCGATTTTAGAAGCAGATGATACAGAAGTATTAGAGATTATGAATGCTGCATATATGATTCGTCATCATTATT
TTGGTAAAAAAGTGAAATTAAATATGATTATTAATACGAAATCGGGATTATGTCCAGAAGATTGTGGTTACTGTTCTCAA
TCAATCGTTTCTGAAGCACCGATTGATAAGTATGCTTGGTTGACACAAGAAAAAATTGTTGAAGGAGCACACGAAGCAGT
TCGCCGAAAAGCTGGTACATATTGTATTGTTGCATCTGGTCGCCGTCCAACAGACAAAGAGGTAAATCATGTCATTGGTG
CGGTGAAAGAAATTAAAGAAACAACGGATTTAAAGATATGTTGTTGCTTAGGCTTTTTAAATGAGGATCAAGCAAAACGA
TTAGCCGAGGCCGGGGTTCACCGTTATAATCATAACTTGAATACACATGCGAATCATTATGATAATATTTGCTCCACACA
CACGTATGATGACCGAGTAGATACGGTAGAAAAAGTGAAGCAAGCGGGCATTTCTCCTTGTTCTGGTGCAATCTTTGGAA
TGGGAGAAACGAAAGAGGAGCGCGTGGAAATTGCTTTTGAGTTACAACGGCTAGATGCAGACTCTATTCCATGTAATTTT
CTCGTCTCTGTAAAAGGCACTCCGTTTGAAGGGCGAAAAGAATTAACGCCAGTGGAATGTTTAAAAATATTAGCGATGAT
GCGTTTTGTAAATCCTTCCAAAGAAATTCGAATTTCTGGAGGCCGTGAACTAAATCTAAGATCTGTACAGCCGTTAGGAT
TATTTGCAGCCAATTCGATTTTCGTTGGAGATTATTTAACAACAGTAGGTCAAGAGTCAACAGCAGACTGGGAGATGATT
CAGGACCTAGGATTTGAAATTGAGGAATGTGCGTTATAA

Upstream 100 bases:

>100_bases
ATGCTTCGCATATATGAGAGAGACTTTACGAAAGAAGGGGAGATTATAGCAACGTACCATGCTTTATTTGCGCACATAGA
AAAAGAGGGGAAGAGGAGAA

Downstream 100 bases:

>100_bases
GTAAGAAAAACCAAGCTTTTAGAGCAAGCTTGGTTTTTGTAATTAAAATGTAATAAAGTGGAAATAGGGTTGTCTGGAAG
CCATTTGCTCCTGGAAATAC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKQIQTKVDWKKIAFEGIEGKRITKEDALAILEADDTEVLEIMNAAYMIRHHYFGKKVKLNMIINTKSGLCPEDCGYCSQ
SIVSEAPIDKYAWLTQEKIVEGAHEAVRRKAGTYCIVASGRRPTDKEVNHVIGAVKEIKETTDLKICCCLGFLNEDQAKR
LAEAGVHRYNHNLNTHANHYDNICSTHTYDDRVDTVEKVKQAGISPCSGAIFGMGETKEERVEIAFELQRLDADSIPCNF
LVSVKGTPFEGRKELTPVECLKILAMMRFVNPSKEIRISGGRELNLRSVQPLGLFAANSIFVGDYLTTVGQESTADWEMI
QDLGFEIEECAL

Sequences:

>Translated_332_residues
MKQIQTKVDWKKIAFEGIEGKRITKEDALAILEADDTEVLEIMNAAYMIRHHYFGKKVKLNMIINTKSGLCPEDCGYCSQ
SIVSEAPIDKYAWLTQEKIVEGAHEAVRRKAGTYCIVASGRRPTDKEVNHVIGAVKEIKETTDLKICCCLGFLNEDQAKR
LAEAGVHRYNHNLNTHANHYDNICSTHTYDDRVDTVEKVKQAGISPCSGAIFGMGETKEERVEIAFELQRLDADSIPCNF
LVSVKGTPFEGRKELTPVECLKILAMMRFVNPSKEIRISGGRELNLRSVQPLGLFAANSIFVGDYLTTVGQESTADWEMI
QDLGFEIEECAL
>Mature_332_residues
MKQIQTKVDWKKIAFEGIEGKRITKEDALAILEADDTEVLEIMNAAYMIRHHYFGKKVKLNMIINTKSGLCPEDCGYCSQ
SIVSEAPIDKYAWLTQEKIVEGAHEAVRRKAGTYCIVASGRRPTDKEVNHVIGAVKEIKETTDLKICCCLGFLNEDQAKR
LAEAGVHRYNHNLNTHANHYDNICSTHTYDDRVDTVEKVKQAGISPCSGAIFGMGETKEERVEIAFELQRLDADSIPCNF
LVSVKGTPFEGRKELTPVECLKILAMMRFVNPSKEIRISGGRELNLRSVQPLGLFAANSIFVGDYLTTVGQESTADWEMI
QDLGFEIEECAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=307, Percent_Identity=34.2019543973941, Blast_Score=194, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321725, Length=310, Percent_Identity=32.5806451612903, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_BACCN (A7GSD8)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001376041.1
- ProteinModelPortal:   A7GSD8
- SMR:   A7GSD8
- STRING:   A7GSD8
- EnsemblBacteria:   EBBACT00000037554
- GeneID:   5343487
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_2813
- eggNOG:   COG0502
- GeneTree:   EBGT00050000002917
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   PRK06256
- BioCyc:   BCER315749:BCER98_2813-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37185; Mature: 37185

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIQTKVDWKKIAFEGIEGKRITKEDALAILEADDTEVLEIMNAAYMIRHHYFGKKVKL
CCCCHHHCCHHHHHHCCCCCCEECHHHCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEE
NMIINTKSGLCPEDCGYCSQSIVSEAPIDKYAWLTQEKIVEGAHEAVRRKAGTYCIVASG
EEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
RRPTDKEVNHVIGAVKEIKETTDLKICCCLGFLNEDQAKRLAEAGVHRYNHNLNTHANHY
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
DNICSTHTYDDRVDTVEKVKQAGISPCSGAIFGMGETKEERVEIAFELQRLDADSIPCNF
HCHHCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCEE
LVSVKGTPFEGRKELTPVECLKILAMMRFVNPSKEIRISGGRELNLRSVQPLGLFAANSI
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCEEEECCE
FVGDYLTTVGQESTADWEMIQDLGFEIEECAL
EEHHHHHHCCCCCCCHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MKQIQTKVDWKKIAFEGIEGKRITKEDALAILEADDTEVLEIMNAAYMIRHHYFGKKVKL
CCCCHHHCCHHHHHHCCCCCCEECHHHCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEE
NMIINTKSGLCPEDCGYCSQSIVSEAPIDKYAWLTQEKIVEGAHEAVRRKAGTYCIVASG
EEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
RRPTDKEVNHVIGAVKEIKETTDLKICCCLGFLNEDQAKRLAEAGVHRYNHNLNTHANHY
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
DNICSTHTYDDRVDTVEKVKQAGISPCSGAIFGMGETKEERVEIAFELQRLDADSIPCNF
HCHHCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCEE
LVSVKGTPFEGRKELTPVECLKILAMMRFVNPSKEIRISGGRELNLRSVQPLGLFAANSI
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCEEEECCE
FVGDYLTTVGQESTADWEMIQDLGFEIEECAL
EEHHHHHHCCCCCCCHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA