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The map label for this gene is ptsH [H]

Identifier: 152976459

GI number: 152976459

Start: 2831846

End: 2832109

Strand: Reverse

Name: ptsH [H]

Synonym: Bcer98_2748

Alternate gene names: 152976459

Gene position: 2832109-2831846 (Counterclockwise)

Preceding gene: 152976460

Following gene: 152976458

Centisome position: 69.3

GC content: 35.98

Gene sequence:

>264_bases
ATGGAAAAAATTTTTAAAGTAACTAGTGACTCAGGAATTCATGCTCGTCCAGCAACTCTACTTGTAAATACAGCAAGTAA
ATTCGGTGCTGACATTAACTTAGAATACAACGGAAAAAATGTTAACTTAAAATCAATCATGGGCGTTATGTCTTTAGGCA
TTCAACAAGGCGCGGAAATTAAAATCACTGCAAATGGTGATGATGCAGCTCAAGCACTAGCAGCTATCGAAGAAACAATG
AAAAACGAAGGATTAGGAGAATAA

Upstream 100 bases:

>100_bases
GTGATGTGGTTGACCGAACATCACCTATTATATAAAATAGATAATGTAGTACTCACGTCTATACAACTAAAAATAATGTA
TTTAAAGGAGATAAATAACT

Downstream 100 bases:

>100_bases
TGACTCTTCATATTCAAGGGATTGCTGCATCAAGTGGGATTGCTATTGCGAAGGCTTTCAGACTTGAGAATCCTGAATTT
AACATTGAAAAGAAAACGAT

Product: phosphocarrier protein HPr

Products: fructose-6-phosphate [Cytoplasm]; pyruvate; fructose-1-phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 87; Mature: 87

Protein sequence:

>87_residues
MEKIFKVTSDSGIHARPATLLVNTASKFGADINLEYNGKNVNLKSIMGVMSLGIQQGAEIKITANGDDAAQALAAIEETM
KNEGLGE

Sequences:

>Translated_87_residues
MEKIFKVTSDSGIHARPATLLVNTASKFGADINLEYNGKNVNLKSIMGVMSLGIQQGAEIKITANGDDAAQALAAIEETM
KNEGLGE
>Mature_87_residues
MEKIFKVTSDSGIHARPATLLVNTASKFGADINLEYNGKNVNLKSIMGVMSLGIQQGAEIKITANGDDAAQALAAIEETM
KNEGLGE

Specific function: P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbo

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=86, Percent_Identity=46.5116279069767, Blast_Score=74, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 9166; Mature: 9166

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIFKVTSDSGIHARPATLLVNTASKFGADINLEYNGKNVNLKSIMGVMSLGIQQGAEI
CCCEEEECCCCCCCCCCEEEEEECHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEE
KITANGDDAAQALAAIEETMKNEGLGE
EEEECCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKIFKVTSDSGIHARPATLLVNTASKFGADINLEYNGKNVNLKSIMGVMSLGIQQGAEI
CCCEEEECCCCCCCCCCEEEEEECHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEE
KITANGDDAAQALAAIEETMKNEGLGE
EEEECCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Fructose [Periplasm]; phosphoenolpyruvate [C]

Specific reaction: phosphoenolpyruvate + fructose [Periplasm] = fructose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + fructose [Periplasm] = fructose-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11168390 [H]