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The map label for this gene is ptsI [H]

Identifier: 152976458

GI number: 152976458

Start: 2830134

End: 2831846

Strand: Reverse

Name: ptsI [H]

Synonym: Bcer98_2747

Alternate gene names: 152976458

Gene position: 2831846-2830134 (Counterclockwise)

Preceding gene: 152976459

Following gene: 152976454

Centisome position: 69.29

GC content: 38.24

Gene sequence:

>1713_bases
ATGACTCTTCATATTCAAGGGATTGCTGCATCAAGTGGGATTGCTATTGCGAAGGCTTTCAGACTTGAGAATCCTGAATT
TAACATTGAAAAGAAAACGATTACGGATGAAGCTGCAGAAATTACACGCTTAGAAGCTGCGTTAGAGAAGGCGAAATCTG
AATTAGAAGCAATCAAAGACCACGCTTTCGCTGAGTTAGGTGCAGATAAAGCGGCTATCTTTGAAGCGCATTTACTAGTA
TTGAATGATCCGGAGTTGGTAAACCCAGTAAAGGATAAAGTGAAAAATGAAAAAGTAAATGCTGAATTCGCAATGGATGA
AGTTGCAACGATGTTTATTACGATGTTTGAAAACATGGATAATGAATATATGAAAGAACGTGCAGCTGACATTCGTGATG
TAACGAAACGTGTTCTTGCACACTTACTAGGAGTTAACTTCTCAAATCCAAGCACAATTTCCGAAGAAGTGATTATTATT
GCTGAAGATTTAACACCATCTGATACGGCACAATTAAATCGCGCATATGCAAAAGGCTTTACAACTGATATCGGTGGACG
TACATCTCACTCTGCAATTATGGCTCGCTCTATGGAAATTCCAGCTGTTGTTGGTACGAAAGTAGCTATGGAGAAAATTC
AAAATGGCGATATCGTAATCATCGATGGATTAGATGGAGAAGTCATTGTAAACCCATCTGAAGAAACTCTTCGTACTTTT
GAAGAAAAGAAAATAAAATTTGAAGAGCAAAAAGCTGAATGGGCGAAATTAAAAAATCAAGCTACTGTGACAAGCGATGG
ACATCATGTTGAACTTGTTGCAAACATCGGTACGCCAAATGATGTACAAGGTATCATTGACAACGGTGGAGAAGGCGTTG
GTTTATACCGCACAGAATTCTTATATATGGGCCGTGACAACCTTCCGACAGAGGAAGAACAGTTCGAAGCATATAAAGCT
GTTCTGGAAGGAGTAAAAGAAGGTCAACCAGTCGTTGTTCGTACACTTGACATTGGTGGAGATAAAGAGCTTCCATACTT
ACATTTACCAAAAGAAATGAACCCATTTTTAGGATACCGTGCAATTCGTTTATGCCTTGAAGAGCAAGATGTGTTCCGCA
CACAGCTTCGTGCATTACTTCGTGCTAGCGTATACGGTAACTTAAAAATTATGTTCCCAATGATTGCAACTCTTGCTGAA
TTCCGTCAAGCGAAAGCAATCTTATTAGAAGAGAAAGAAAAGCTTGTTAAAGCTGGTACAACAGTTTCTGATTCTATTGA
AGTAGGTATGATGGTAGAAATACCAGCTTCGGCAGTATTAGCAGATCAATTTGCTAAAGAAGTTGATTTCTTCTCAATCG
GAACTAATGATTTAATTCAATACACAATGGCTGCGGATCGCATGAATGAACGTGTATCTTACTTGTATCAACCATATAAT
CCAGCTATTTTACGTCTTGTAAAAATGGTAATCGATGCTGCTCATAAAGAAGGAAAATGGGCTGGTATGTGTGGTGAGAT
GGCAGGAGATTCACTTGCAATCCCGCTATTATTAGGTTTAGGATTAGATGAATTTAGTATGAGTGCAACATCTATCCTTC
CTGCAAGAACACAATTAAGCAAATTGTCAAAAGCAGAAATGGAAGAGTTAGCACAAAAAGCATTAATGATGTCAACGGCT
GAAGAAGTTGTTGAATTAGTAAAAAGTATTTAA

Upstream 100 bases:

>100_bases
AACAAGGCGCGGAAATTAAAATCACTGCAAATGGTGATGATGCAGCTCAAGCACTAGCAGCTATCGAAGAAACAATGAAA
AACGAAGGATTAGGAGAATA

Downstream 100 bases:

>100_bases
GTACAAAAAACCTGAGGCCAATGAAATCGGTCTCAGGGCTTTTTTTGTACATAAAGTAGACAAGAGATTATTAGATAGCG
ATAGCAACAATATCATCTAG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MTLHIQGIAASSGIAIAKAFRLENPEFNIEKKTITDEAAEITRLEAALEKAKSELEAIKDHAFAELGADKAAIFEAHLLV
LNDPELVNPVKDKVKNEKVNAEFAMDEVATMFITMFENMDNEYMKERAADIRDVTKRVLAHLLGVNFSNPSTISEEVIII
AEDLTPSDTAQLNRAYAKGFTTDIGGRTSHSAIMARSMEIPAVVGTKVAMEKIQNGDIVIIDGLDGEVIVNPSEETLRTF
EEKKIKFEEQKAEWAKLKNQATVTSDGHHVELVANIGTPNDVQGIIDNGGEGVGLYRTEFLYMGRDNLPTEEEQFEAYKA
VLEGVKEGQPVVVRTLDIGGDKELPYLHLPKEMNPFLGYRAIRLCLEEQDVFRTQLRALLRASVYGNLKIMFPMIATLAE
FRQAKAILLEEKEKLVKAGTTVSDSIEVGMMVEIPASAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYN
PAILRLVKMVIDAAHKEGKWAGMCGEMAGDSLAIPLLLGLGLDEFSMSATSILPARTQLSKLSKAEMEELAQKALMMSTA
EEVVELVKSI

Sequences:

>Translated_570_residues
MTLHIQGIAASSGIAIAKAFRLENPEFNIEKKTITDEAAEITRLEAALEKAKSELEAIKDHAFAELGADKAAIFEAHLLV
LNDPELVNPVKDKVKNEKVNAEFAMDEVATMFITMFENMDNEYMKERAADIRDVTKRVLAHLLGVNFSNPSTISEEVIII
AEDLTPSDTAQLNRAYAKGFTTDIGGRTSHSAIMARSMEIPAVVGTKVAMEKIQNGDIVIIDGLDGEVIVNPSEETLRTF
EEKKIKFEEQKAEWAKLKNQATVTSDGHHVELVANIGTPNDVQGIIDNGGEGVGLYRTEFLYMGRDNLPTEEEQFEAYKA
VLEGVKEGQPVVVRTLDIGGDKELPYLHLPKEMNPFLGYRAIRLCLEEQDVFRTQLRALLRASVYGNLKIMFPMIATLAE
FRQAKAILLEEKEKLVKAGTTVSDSIEVGMMVEIPASAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYN
PAILRLVKMVIDAAHKEGKWAGMCGEMAGDSLAIPLLLGLGLDEFSMSATSILPARTQLSKLSKAEMEELAQKALMMSTA
EEVVELVKSI
>Mature_569_residues
TLHIQGIAASSGIAIAKAFRLENPEFNIEKKTITDEAAEITRLEAALEKAKSELEAIKDHAFAELGADKAAIFEAHLLVL
NDPELVNPVKDKVKNEKVNAEFAMDEVATMFITMFENMDNEYMKERAADIRDVTKRVLAHLLGVNFSNPSTISEEVIIIA
EDLTPSDTAQLNRAYAKGFTTDIGGRTSHSAIMARSMEIPAVVGTKVAMEKIQNGDIVIIDGLDGEVIVNPSEETLRTFE
EKKIKFEEQKAEWAKLKNQATVTSDGHHVELVANIGTPNDVQGIIDNGGEGVGLYRTEFLYMGRDNLPTEEEQFEAYKAV
LEGVKEGQPVVVRTLDIGGDKELPYLHLPKEMNPFLGYRAIRLCLEEQDVFRTQLRALLRASVYGNLKIMFPMIATLAEF
RQAKAILLEEKEKLVKAGTTVSDSIEVGMMVEIPASAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
AILRLVKMVIDAAHKEGKWAGMCGEMAGDSLAIPLLLGLGLDEFSMSATSILPARTQLSKLSKAEMEELAQKALMMSTAE
EVVELVKSI

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=565, Percent_Identity=50.4424778761062, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI48994992, Length=516, Percent_Identity=39.7286821705426, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI1788726, Length=568, Percent_Identity=35.387323943662, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI1789193, Length=574, Percent_Identity=33.1010452961672, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1787994, Length=432, Percent_Identity=28.0092592592593, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI226510935, Length=205, Percent_Identity=28.780487804878, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 62957; Mature: 62826

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLHIQGIAASSGIAIAKAFRLENPEFNIEKKTITDEAAEITRLEAALEKAKSELEAIKD
CEEEEEEEECCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFAELGADKAAIFEAHLLVLNDPELVNPVKDKVKNEKVNAEFAMDEVATMFITMFENMD
HHHHHHCCCHHEEEEEEEEEECCCHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
NEYMKERAADIRDVTKRVLAHLLGVNFSNPSTISEEVIIIAEDLTPSDTAQLNRAYAKGF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCC
TTDIGGRTSHSAIMARSMEIPAVVGTKVAMEKIQNGDIVIIDGLDGEVIVNPSEETLRTF
CCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHH
EEKKIKFEEQKAEWAKLKNQATVTSDGHHVELVANIGTPNDVQGIIDNGGEGVGLYRTEF
HHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEECCCCCCCHHHHCCCCCCEEEEEEEE
LYMGRDNLPTEEEQFEAYKAVLEGVKEGQPVVVRTLDIGGDKELPYLHLPKEMNPFLGYR
EEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHH
AIRLCLEEQDVFRTQLRALLRASVYGNLKIMFPMIATLAEFRQAKAILLEEKEKLVKAGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TVSDSIEVGMMVEIPASAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYN
CCCCCCCCCEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
PAILRLVKMVIDAAHKEGKWAGMCGEMAGDSLAIPLLLGLGLDEFSMSATSILPARTQLS
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEHHHHHHCCCCHHHCCHHHHCCHHHHHH
KLSKAEMEELAQKALMMSTAEEVVELVKSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLHIQGIAASSGIAIAKAFRLENPEFNIEKKTITDEAAEITRLEAALEKAKSELEAIKD
EEEEEEEECCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFAELGADKAAIFEAHLLVLNDPELVNPVKDKVKNEKVNAEFAMDEVATMFITMFENMD
HHHHHHCCCHHEEEEEEEEEECCCHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
NEYMKERAADIRDVTKRVLAHLLGVNFSNPSTISEEVIIIAEDLTPSDTAQLNRAYAKGF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCC
TTDIGGRTSHSAIMARSMEIPAVVGTKVAMEKIQNGDIVIIDGLDGEVIVNPSEETLRTF
CCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHH
EEKKIKFEEQKAEWAKLKNQATVTSDGHHVELVANIGTPNDVQGIIDNGGEGVGLYRTEF
HHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEECCCCCCCHHHHCCCCCCEEEEEEEE
LYMGRDNLPTEEEQFEAYKAVLEGVKEGQPVVVRTLDIGGDKELPYLHLPKEMNPFLGYR
EEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHH
AIRLCLEEQDVFRTQLRALLRASVYGNLKIMFPMIATLAEFRQAKAILLEEKEKLVKAGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TVSDSIEVGMMVEIPASAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYN
CCCCCCCCCEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
PAILRLVKMVIDAAHKEGKWAGMCGEMAGDSLAIPLLLGLGLDEFSMSATSILPARTQLS
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEHHHHHHCCCCHHHCCHHHHCCHHHHHH
KLSKAEMEELAQKALMMSTAEEVVELVKSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA