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The map label for this gene is pdhC [H]

Identifier: 152976384

GI number: 152976384

Start: 2756407

End: 2757672

Strand: Reverse

Name: pdhC [H]

Synonym: Bcer98_2672

Alternate gene names: 152976384

Gene position: 2757672-2756407 (Counterclockwise)

Preceding gene: 152976385

Following gene: 152976383

Centisome position: 67.47

GC content: 40.36

Gene sequence:

>1266_bases
GTGGCATTTGAATTTAAACTACCAGATATCGGTGAAGGTATCCACGAAGGTGAAATCGTAAAATGGTTTATTAAACCAGG
CGATGAAGTAAACGAAGACGACGTACTTCTTGAAGTACAAAATGATAAAGCAGTAGTAGAAATCCCTTCTCCTGTAAAAG
GTAAAGTACTTGAAGTACTTGTAGAAGAAGGAACAGTTGCAGTAGTAGGAGATGTTCTTGTTAAATTTGATGCTCCTGGT
TACGAAAACCTTAAATTTAAAGGTGACGAGCATGACGATGCTCCAAAAGCTGAGGAAGCAAAAGAAGAAGCTCCAGCAGC
AGCTACTCCAGTAGCAGAAACAACAAATGAACGCGTAATCGCTATGCCATCTGTTCGTAAATATGCTCGTGAAAAAGGTG
TAGACATCCATAAAGTGGCAGGATCTGGTAAAAACGGTCGCGTTGTAAAAGCTGACATTGATTCATTTGTAAATGGTGGA
CAAGCAGCGACTACTGAAGCTGCAGCAGAAGCTCCAGCAGCTCAAGAAGAAGCGCCAAAAGCACAACCAATCCCAGCTGG
TGAATACCCAGAAACTCGCGAGAAAATGAGCGGTATCCGTAAAGCGATTGCGAAAGCAATGGTTAACTCTAAACATACAG
CTCCTCACGTAACATTAATGGACGAAGTAGATGTAACAGAGCTTGTTGCTCATCGTAAGAAATTCAAAGCTGTTGCAGCT
GACAAAGGTATTAAATTAACTTACCTTCCATATGTTGTAAAAGCTTTAACATCTGCATTACGTGAATTCCCAATGTTGAA
CACTTCTTTAGATGATGCAACTCAAGAAATTGTTCATAAACATTACTTCAATATTGGTATCGCAGCTGATACTGATAAAG
GTCTTTTAGTACCAGTTGTTAAAGATGCTGATCGCAAGTCTATCTTTGCTATCTCTAGTGAGATTAATGAGCTTGCTACA
AAAGCGCGTGAAGGTCGCTTAGCTCCTGCTGAAATGAAAGGTGCTTCTTGCACAATCACAAACATTGGTTCTGCTGGTGG
ACAATGGTTCACTCCAGTTATCAATCACCCAGAAGTAGCAATCCTTGGTATTGGTCGCATCGCTGAAAAACCAGTTGTGA
AAAATGGTGAGATCGTTGCAGCTCCAGTATTAGCATTATCTCTAAGCTTTGACCATCGTTTAATTGACGGTGCAACTGCT
CAAAAAGCATTAAACCAAATTAAACGTCTATTAAACGACCCACAATTATTAGTAATGGAGGCGTAA

Upstream 100 bases:

>100_bases
ACTTCTAATTTTAGTTATTCATGTGCAAAAGAAGTCAGCACATGTTGATTTCTTTTGTACATATTCCATAATAATGAAAT
GAATCAGGGGGATGAATTCC

Downstream 100 bases:

>100_bases
TAACAATGGTAGTAGGAGATTTCCCAATTGAATTAGATACAGTCGTTGTTGGTGCAGGTCCTGGTGGATATGTTGCAGCA
ATTCGTGCAGCACAATTAGG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MAFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVLVEEGTVAVVGDVLVKFDAPG
YENLKFKGDEHDDAPKAEEAKEEAPAAATPVAETTNERVIAMPSVRKYAREKGVDIHKVAGSGKNGRVVKADIDSFVNGG
QAATTEAAAEAPAAQEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRKKFKAVAA
DKGIKLTYLPYVVKALTSALREFPMLNTSLDDATQEIVHKHYFNIGIAADTDKGLLVPVVKDADRKSIFAISSEINELAT
KAREGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATA
QKALNQIKRLLNDPQLLVMEA

Sequences:

>Translated_421_residues
MAFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVLVEEGTVAVVGDVLVKFDAPG
YENLKFKGDEHDDAPKAEEAKEEAPAAATPVAETTNERVIAMPSVRKYAREKGVDIHKVAGSGKNGRVVKADIDSFVNGG
QAATTEAAAEAPAAQEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRKKFKAVAA
DKGIKLTYLPYVVKALTSALREFPMLNTSLDDATQEIVHKHYFNIGIAADTDKGLLVPVVKDADRKSIFAISSEINELAT
KAREGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATA
QKALNQIKRLLNDPQLLVMEA
>Mature_420_residues
AFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVLVEEGTVAVVGDVLVKFDAPGY
ENLKFKGDEHDDAPKAEEAKEEAPAAATPVAETTNERVIAMPSVRKYAREKGVDIHKVAGSGKNGRVVKADIDSFVNGGQ
AATTEAAAEAPAAQEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRKKFKAVAAD
KGIKLTYLPYVVKALTSALREFPMLNTSLDDATQEIVHKHYFNIGIAADTDKGLLVPVVKDADRKSIFAISSEINELATK
AREGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATAQ
KALNQIKRLLNDPQLLVMEA

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=35.4312354312354, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=30.8045977011494, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI19923748, Length=235, Percent_Identity=36.1702127659575, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=27.9642058165548, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI203098816, Length=447, Percent_Identity=27.9642058165548, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=35, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786305, Length=428, Percent_Identity=35.5140186915888, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786946, Length=426, Percent_Identity=32.1596244131455, Blast_Score=211, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=33.0985915492958, Blast_Score=230, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI25146366, Length=416, Percent_Identity=32.6923076923077, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=30.3370786516854, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=30.1948051948052, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=416, Percent_Identity=31.25, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=28.2511210762332, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18859875, Length=432, Percent_Identity=36.5740740740741, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=33.4763948497854, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=30.7017543859649, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=30.7017543859649, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45232; Mature: 45101

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVL
CCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHH
VEEGTVAVVGDVLVKFDAPGYENLKFKGDEHDDAPKAEEAKEEAPAAATPVAETTNERVI
HHCCCEEEEEEEEEEECCCCCCCCEECCCCCCCCCCHHHHHHHCCCCCCCCHHCCCCEEE
AMPSVRKYAREKGVDIHKVAGSGKNGRVVKADIDSFVNGGQAATTEAAAEAPAAQEEAPK
ECCHHHHHHHHCCCEEEEECCCCCCCCEEEECHHHHHCCCCCCCHHHHHCCCCCHHCCCC
AQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRKKFKAVAA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHC
DKGIKLTYLPYVVKALTSALREFPMLNTSLDDATQEIVHKHYFNIGIAADTDKGLLVPVV
CCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEEEEE
KDADRKSIFAISSEINELATKAREGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVA
CCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCEECCCCCCCCEE
ILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATAQKALNQIKRLLNDPQLLVME
EEECCHHCCCCCCCCCCEEEEEHEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCEEEEE
A
C
>Mature Secondary Structure 
AFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVL
CEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHH
VEEGTVAVVGDVLVKFDAPGYENLKFKGDEHDDAPKAEEAKEEAPAAATPVAETTNERVI
HHCCCEEEEEEEEEEECCCCCCCCEECCCCCCCCCCHHHHHHHCCCCCCCCHHCCCCEEE
AMPSVRKYAREKGVDIHKVAGSGKNGRVVKADIDSFVNGGQAATTEAAAEAPAAQEEAPK
ECCHHHHHHHHCCCEEEEECCCCCCCCEEEECHHHHHCCCCCCCHHHHHCCCCCHHCCCC
AQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRKKFKAVAA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHC
DKGIKLTYLPYVVKALTSALREFPMLNTSLDDATQEIVHKHYFNIGIAADTDKGLLVPVV
CCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEEEEE
KDADRKSIFAISSEINELATKAREGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVA
CCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCEECCCCCCCCEE
ILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATAQKALNQIKRLLNDPQLLVME
EEECCHHCCCCCCCCCCEEEEEHEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCEEEEE
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]