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The map label for this gene is pdhD [H]

Identifier: 152976383

GI number: 152976383

Start: 2754989

End: 2756401

Strand: Reverse

Name: pdhD [H]

Synonym: Bcer98_2671

Alternate gene names: 152976383

Gene position: 2756401-2754989 (Counterclockwise)

Preceding gene: 152976384

Following gene: 152976382

Centisome position: 67.44

GC content: 40.27

Gene sequence:

>1413_bases
ATGGTAGTAGGAGATTTCCCAATTGAATTAGATACAGTCGTTGTTGGTGCAGGTCCTGGTGGATATGTTGCAGCAATTCG
TGCAGCACAATTAGGTCAAAAGGTAGCAGTTATTGAAAAAGCTAACCTTGGAGGCGTATGCTTAAACGTTGGATGTATTC
CTTCTAAAGCGTTAATTAATGCAGGTCATCGTTTTGAGCATGCAAAACATTCTGATGACATGGGTATTATCGCTGAAAAT
GTAACAGTGGACTTCACAAAAGTTCAAGAATGGAAAAATGGCGTAGTTAAGAAATTAACTGGCGGTGTAGAAGGCCTTCT
TAAAGGAAACAAAGTTGAAATCATTCGCGGTGAAGCTTACTTCGTAGATGCAAATACATTACGTGTAATGACTGAAGATG
CAGCACAAACTTATACATTCAAAAATGCAATCCTTGCAACTGGTTCTACACCAATTGAAATTCCAGGATTCAAATATTCT
AAACGCGTTATCAACTCTACAGGTGCTTTAAGTTTGCCTGAAATTCCTAAAAAACTTGTTGTAATCGGCGGCGGTTACAT
CGGTATGGAATTAGGTACTGCTTATGCTAACTTCGGTACAGAAGTTACTGTAGTAGAAGCTGGTGACGAAATCTTAGCTG
GTTTCGAAAAAGCAATGAGCTCAGTTGTAAAACGTGCTCTTCAGAAAAAAGGAAACGTTACAATCCATACGAAAGCTATG
GCTAAAGGCGTTGAAGAAACTGAAAACGGCGTAAAAGTTAGCTTCGAAGTAAAAGGCGAAGTACAAACTGTAGAAGCAGA
TTACGTATTAGTAACTGTAGGTCGTCGTCCAAATACTCAAGAAATCGGTCTTGAGCAACTTGGAATTAAAATGACTGATC
GCGGTCTAATCGAAATCGATGAGCAATGCCGTACAAATGTACCAAACATCTATGCAATCGGTGATATCGTTCCTGGACCA
CCATTAGCGCACAAAGCTTCTTACGAAGGTAAAGTAGCAGCTGAAGCTATTAGTGGTCATGCAGCAGCAATCGATTACAT
TGGAATTCCAGCAGTATGCTTCACTGATCCAGAATTAGCATCTGTTGGTTATACGAAGAAACAAGCAGAAGAAGCTGGAA
TGAGCGTAGCTGTATCTAAATTCCCATTCGCTGCAAACGGTCGTGCATTATCATTAAACAGCACAGACGGTTTCTTACAA
CTTGTAACACGTAAAGAAGATGGTCTTCTTGTAGGTGCACAAGTTGCAGGTGCAGGTGCTTCTGATATTATCTCTGAGTT
AGGACTTGCTATCGAAGCGGGTATGACAGCAGAAGATATTGCACAAACAATCCATGCTCACCCAACATTAGGTGAAATTA
CAATGGAAGCAGCAGAAGTTGCTCTTGGAATGCCAATTCACATTGTAAAATAA

Upstream 100 bases:

>100_bases
ACCATCGTTTAATTGACGGTGCAACTGCTCAAAAAGCATTAAACCAAATTAAACGTCTATTAAACGACCCACAATTATTA
GTAATGGAGGCGTAATAACA

Downstream 100 bases:

>100_bases
TATAGTAAGTAAGAAAAAAACGTCTCTTATTCTGTAAGAGGCGTTTTTTTCTTATAAGGTCTTTATCCCGCTATTTTAGG
GGAGTAGTAGCCTGCTTGGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAVIEKANLGGVCLNVGCIPSKALINAGHRFEHAKHSDDMGIIAEN
VTVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAYFVDANTLRVMTEDAAQTYTFKNAILATGSTPIEIPGFKYS
KRVINSTGALSLPEIPKKLVVIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVTIHTKAM
AKGVEETENGVKVSFEVKGEVQTVEADYVLVTVGRRPNTQEIGLEQLGIKMTDRGLIEIDEQCRTNVPNIYAIGDIVPGP
PLAHKASYEGKVAAEAISGHAAAIDYIGIPAVCFTDPELASVGYTKKQAEEAGMSVAVSKFPFAANGRALSLNSTDGFLQ
LVTRKEDGLLVGAQVAGAGASDIISELGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK

Sequences:

>Translated_470_residues
MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAVIEKANLGGVCLNVGCIPSKALINAGHRFEHAKHSDDMGIIAEN
VTVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAYFVDANTLRVMTEDAAQTYTFKNAILATGSTPIEIPGFKYS
KRVINSTGALSLPEIPKKLVVIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVTIHTKAM
AKGVEETENGVKVSFEVKGEVQTVEADYVLVTVGRRPNTQEIGLEQLGIKMTDRGLIEIDEQCRTNVPNIYAIGDIVPGP
PLAHKASYEGKVAAEAISGHAAAIDYIGIPAVCFTDPELASVGYTKKQAEEAGMSVAVSKFPFAANGRALSLNSTDGFLQ
LVTRKEDGLLVGAQVAGAGASDIISELGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK
>Mature_470_residues
MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAVIEKANLGGVCLNVGCIPSKALINAGHRFEHAKHSDDMGIIAEN
VTVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAYFVDANTLRVMTEDAAQTYTFKNAILATGSTPIEIPGFKYS
KRVINSTGALSLPEIPKKLVVIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVTIHTKAM
AKGVEETENGVKVSFEVKGEVQTVEADYVLVTVGRRPNTQEIGLEQLGIKMTDRGLIEIDEQCRTNVPNIYAIGDIVPGP
PLAHKASYEGKVAAEAISGHAAAIDYIGIPAVCFTDPELASVGYTKKQAEEAGMSVAVSKFPFAANGRALSLNSTDGFLQ
LVTRKEDGLLVGAQVAGAGASDIISELGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=463, Percent_Identity=43.4125269978402, Blast_Score=351, Evalue=1e-96,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=30.4157549234136, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI33519430, Length=456, Percent_Identity=27.6315789473684, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI33519428, Length=456, Percent_Identity=27.6315789473684, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI33519426, Length=456, Percent_Identity=27.6315789473684, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI291045266, Length=460, Percent_Identity=28.4782608695652, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI148277065, Length=456, Percent_Identity=27.6315789473684, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI148277071, Length=456, Percent_Identity=27.6315789473684, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=28.0434782608696, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=26.6075388026608, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1786307, Length=459, Percent_Identity=44.4444444444444, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=29.4372294372294, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=30.6818181818182, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI87081717, Length=447, Percent_Identity=29.082774049217, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=42.5764192139738, Blast_Score=350, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI71983419, Length=464, Percent_Identity=29.5258620689655, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71983429, Length=464, Percent_Identity=29.5258620689655, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17557007, Length=470, Percent_Identity=28.7234042553192, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=486, Percent_Identity=25.1028806584362, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17559934, Length=202, Percent_Identity=28.7128712871287, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=40.6779661016949, Blast_Score=323, Evalue=3e-89,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=29.3103448275862, Blast_Score=187, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=27.1966527196653, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=44.136460554371, Blast_Score=355, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24640549, Length=467, Percent_Identity=28.2655246252677, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640553, Length=467, Percent_Identity=28.2655246252677, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640551, Length=467, Percent_Identity=28.2655246252677, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17737741, Length=469, Percent_Identity=25.5863539445629, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49355; Mature: 49355

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAVIEKANLGGVCLNVGCIPSKALIN
CEECCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCHHHHC
AGHRFEHAKHSDDMGIIAENVTVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAY
CCCHHCCCCCCCCCEEEEECEEEEHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCEE
FVDANTLRVMTEDAAQTYTFKNAILATGSTPIEIPGFKYSKRVINSTGALSLPEIPKKLV
EEECCEEEEEECCCHHEEEECCEEEECCCCCEECCCCCHHHHHHCCCCCCCCCCCCCEEE
VIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVTIHTKAM
EEECCEEEEECCCHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
AKGVEETENGVKVSFEVKGEVQTVEADYVLVTVGRRPNTQEIGLEQLGIKMTDRGLIEID
HCCHHHCCCCEEEEEEECCCEEEEEECEEEEEECCCCCCHHCCHHHCCCEECCCCCEEEC
EQCRTNVPNIYAIGDIVPGPPLAHKASYEGKVAAEAISGHAAAIDYIGIPAVCFTDPELA
HHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCEEEEEECCCCEEEECCCCHH
SVGYTKKQAEEAGMSVAVSKFPFAANGRALSLNSTDGFLQLVTRKEDGLLVGAQVAGAGA
HCCCCHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCH
SDIISELGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK
HHHHHHHCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAVIEKANLGGVCLNVGCIPSKALIN
CEECCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCHHHHC
AGHRFEHAKHSDDMGIIAENVTVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAY
CCCHHCCCCCCCCCEEEEECEEEEHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCEE
FVDANTLRVMTEDAAQTYTFKNAILATGSTPIEIPGFKYSKRVINSTGALSLPEIPKKLV
EEECCEEEEEECCCHHEEEECCEEEECCCCCEECCCCCHHHHHHCCCCCCCCCCCCCEEE
VIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVTIHTKAM
EEECCEEEEECCCHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
AKGVEETENGVKVSFEVKGEVQTVEADYVLVTVGRRPNTQEIGLEQLGIKMTDRGLIEID
HCCHHHCCCCEEEEEEECCCEEEEEECEEEEEECCCCCCHHCCHHHCCCEECCCCCEEEC
EQCRTNVPNIYAIGDIVPGPPLAHKASYEGKVAAEAISGHAAAIDYIGIPAVCFTDPELA
HHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCEEEEEECCCCEEEECCCCHH
SVGYTKKQAEEAGMSVAVSKFPFAANGRALSLNSTDGFLQLVTRKEDGLLVGAQVAGAGA
HCCCCHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCH
SDIISELGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK
HHHHHHHCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]