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The map label for this gene is rlmN [H]

Identifier: 152976230

GI number: 152976230

Start: 2614987

End: 2616075

Strand: Reverse

Name: rlmN [H]

Synonym: Bcer98_2516

Alternate gene names: 152976230

Gene position: 2616075-2614987 (Counterclockwise)

Preceding gene: 152976231

Following gene: 152976229

Centisome position: 64.01

GC content: 38.48

Gene sequence:

>1089_bases
ATGGAAACAACTACAAGAAAACAAAAGAAAGCTTTAGAAACAAAGAAACCATCTATTTACTCTTTACAACTTCATGAAAT
GCAAGAGTGGTTAAAGGAACAAGGAGAGCCAAAGTTTCGCGCTGGACAAATTTTTGATTGGCTTTACAAAAAACGTGTAA
AAAATTATGAGGATATGACAAATCTTTCTAAAGGATTGCGTGAAAAATTGTCGAATTCCTTTGATATTACTACCTTAAAT
ACATTAGTAAAACAAACCTCTTCTGATGGTACGATTAAATTTTTGTTCCAATTATACGATGGATATTCTATTGAAACAGT
ATTGATGCGACATGAGTATGGAAATTCTATTTGTGTAACAACTCAGGTAGGTTGCCGCATTGGTTGTACGTTCTGTGCGT
CAACATTAGGAGGCCTAAAACGAAACCTAGAGGCTGGGGAAATCGTTGCACAAGTAGTAGAAGTACAGCGTGCGCTTGAT
GAAACAAATGAACGCGTAAGCTCCCTTGTCGTAATGGGAATTGGTGAACCGTTTGACAACTATGACCACTTAATGTCATT
CTTGCGCATTGTTAACCATGAAAAGGGAATCAATATTGGAGCACGTCATATGACTGTTTCAACGAGTGGAATTATCCCGA
AAATTTATAAGTTTGCCGAAGAAGATTTGCAAATTAACTTTGCGATTTCATTGCACGCTCCTAACACAGAATTGCGTTCA
AAATTAATGCCGATTAACCGCGCTTATAAATTACCGGATTTAATGGAAGCTATTAAGTACTATATCAATAGAACAGGGCG
CCGTGTTACGTTCGAGTACGGTTTGTTTGGTGGAGAGAACGATCAAGTGGAGCACGCTGAAGAGCTGGCACAACTTTTAA
AAGGTGTAAAATGTCATGTGAATTTAATTCCGGTAAACTATGTACCAGAGCGAGACTACGTCAGAACACCACGAGAACAA
ATTTTCTTATTTGAAAAAACGTTAAAAAATCGTGGTGTGAATGTAACGATTCGCCGTGAACAAGGTCATGATATTGATGC
TGCCTGCGGTCAGTTGCGTGCAAAGGAGCGCAAAGAAGAGACGAGGTGA

Upstream 100 bases:

>100_bases
TTACGTCCATATATCAATAAAGGACAAGTGCAAATTTTACCGCATTATTTTGCGACGGATGGCTTTTATATTGCTTGTTT
AAGAAAGAAGGTGTAGCATC

Downstream 100 bases:

>100_bases
CAAAATGAAAGCCGTGTTTCTATCGGATAAAGGGAAGGTGCGTCAACACAATGAAGATAGTGCGGGGGTTTTTCACAATT
TAGATGGAGATATTTTAGCT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
METTTRKQKKALETKKPSIYSLQLHEMQEWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMTNLSKGLREKLSNSFDITTLN
TLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALD
ETNERVSSLVVMGIGEPFDNYDHLMSFLRIVNHEKGINIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNTELRS
KLMPINRAYKLPDLMEAIKYYINRTGRRVTFEYGLFGGENDQVEHAEELAQLLKGVKCHVNLIPVNYVPERDYVRTPREQ
IFLFEKTLKNRGVNVTIRREQGHDIDAACGQLRAKERKEETR

Sequences:

>Translated_362_residues
METTTRKQKKALETKKPSIYSLQLHEMQEWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMTNLSKGLREKLSNSFDITTLN
TLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALD
ETNERVSSLVVMGIGEPFDNYDHLMSFLRIVNHEKGINIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNTELRS
KLMPINRAYKLPDLMEAIKYYINRTGRRVTFEYGLFGGENDQVEHAEELAQLLKGVKCHVNLIPVNYVPERDYVRTPREQ
IFLFEKTLKNRGVNVTIRREQGHDIDAACGQLRAKERKEETR
>Mature_362_residues
METTTRKQKKALETKKPSIYSLQLHEMQEWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMTNLSKGLREKLSNSFDITTLN
TLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALD
ETNERVSSLVVMGIGEPFDNYDHLMSFLRIVNHEKGINIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNTELRS
KLMPINRAYKLPDLMEAIKYYINRTGRRVTFEYGLFGGENDQVEHAEELAQLLKGVKCHVNLIPVNYVPERDYVRTPREQ
IFLFEKTLKNRGVNVTIRREQGHDIDAACGQLRAKERKEETR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=344, Percent_Identity=36.9186046511628, Blast_Score=244, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41625; Mature: 41625

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTTRKQKKALETKKPSIYSLQLHEMQEWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMT
CCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
NLSKGLREKLSNSFDITTLNTLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVT
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHEEEECCCCCEEEEE
TQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALDETNERVSSLVVMGIGEPFDN
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
YDHLMSFLRIVNHEKGINIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNTELRS
HHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCEEEEEEEEEECCCHHHHH
KLMPINRAYKLPDLMEAIKYYINRTGRRVTFEYGLFGGENDQVEHAEELAQLLKGVKCHV
HHCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEE
NLIPVNYVPERDYVRTPREQIFLFEKTLKNRGVNVTIRREQGHDIDAACGQLRAKERKEE
EEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
TR
CC
>Mature Secondary Structure
METTTRKQKKALETKKPSIYSLQLHEMQEWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMT
CCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
NLSKGLREKLSNSFDITTLNTLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVT
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHEEEECCCCCEEEEE
TQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALDETNERVSSLVVMGIGEPFDN
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
YDHLMSFLRIVNHEKGINIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNTELRS
HHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCEEEEEEEEEECCCHHHHH
KLMPINRAYKLPDLMEAIKYYINRTGRRVTFEYGLFGGENDQVEHAEELAQLLKGVKCHV
HHCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEE
NLIPVNYVPERDYVRTPREQIFLFEKTLKNRGVNVTIRREQGHDIDAACGQLRAKERKEE
EEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
TR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA