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The map label for this gene is trmD [H]

Identifier: 152976207

GI number: 152976207

Start: 2591521

End: 2592258

Strand: Reverse

Name: trmD [H]

Synonym: Bcer98_2493

Alternate gene names: 152976207

Gene position: 2592258-2591521 (Counterclockwise)

Preceding gene: 152976208

Following gene: 152976206

Centisome position: 63.43

GC content: 40.11

Gene sequence:

>738_bases
ATGATGAAAATTGACATTTTAACACTATTTCCAGAGATGTTCACCGGTGTATTTGGGTCTTCCATTTTAAAGAAAGCGCA
AGAAAAAGAAGCGGTTGAACTTCGGGTCGTAAATTTTCGTGATTATACAACAAATAAGCATAATAGTGTAGATGATTATC
CGTACGGTGGGGGCGCTGGTATGGTATTAACGCCCCAGCCTATATTTGATGCAGTAGAAGATTTAACAAAAGAAACGACA
CAAAAGCCAAGAATTGTATTAATGTGCCCACAAGGGGAAAGGTTCACACAAAAAAAAGCTGAAGAATTAGCTAAGGAAGA
GCATGTGATCTTCGTATGTGGTCATTATGAAGGGTACGATGAGCGCATTCGTGAGCATCTAGTAACGGATGAAATTTCCA
TTGGTGACTACGTATTAACTGGCGGAGAATTAGCGTCAATGGTCATAACAGATAGCGTTGTACGTCTTTTACCAGGAGTA
TTAGGAAATCAAAACTCACAAGTAGAAGATTCTTTTAGTACTGGATTACTAGAACATCCACATTATACGCGTCCGGCTGA
CTTTCGCGGGATGAAGGTGCCGGATGTACTTATGTCAGGAAACCATAAAAAAATTGATGAATGGCGCCATAAGGAATCGT
TGCGTCGTACGTATACGCGTAGACCGGATTTACTGGAAGGGCGCGAGCTAACAAAACAAGAAGAAATATGGCTTGAGCAA
ATAAAAGAAGAACAATAA

Upstream 100 bases:

>100_bases
CAAAAGGACAAGATTTGTTAATTCCATACATTGATGATATTGTATTGCAGGTTAATGTGGAACAAAAACAAGTGACCATT
CATGTAATGGAAGGATTGCT

Downstream 100 bases:

>100_bases
AAACTTCATATATTTTCTATTGCAACAGGCCTAGCAATATGCTATCATAACATTTGTGCTACTGAAGGCAGTAGCTATAT
TAACGATGTTCCGCTGTAAT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MMKIDILTLFPEMFTGVFGSSILKKAQEKEAVELRVVNFRDYTTNKHNSVDDYPYGGGAGMVLTPQPIFDAVEDLTKETT
QKPRIVLMCPQGERFTQKKAEELAKEEHVIFVCGHYEGYDERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGV
LGNQNSQVEDSFSTGLLEHPHYTRPADFRGMKVPDVLMSGNHKKIDEWRHKESLRRTYTRRPDLLEGRELTKQEEIWLEQ
IKEEQ

Sequences:

>Translated_245_residues
MMKIDILTLFPEMFTGVFGSSILKKAQEKEAVELRVVNFRDYTTNKHNSVDDYPYGGGAGMVLTPQPIFDAVEDLTKETT
QKPRIVLMCPQGERFTQKKAEELAKEEHVIFVCGHYEGYDERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGV
LGNQNSQVEDSFSTGLLEHPHYTRPADFRGMKVPDVLMSGNHKKIDEWRHKESLRRTYTRRPDLLEGRELTKQEEIWLEQ
IKEEQ
>Mature_245_residues
MMKIDILTLFPEMFTGVFGSSILKKAQEKEAVELRVVNFRDYTTNKHNSVDDYPYGGGAGMVLTPQPIFDAVEDLTKETT
QKPRIVLMCPQGERFTQKKAEELAKEEHVIFVCGHYEGYDERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGV
LGNQNSQVEDSFSTGLLEHPHYTRPADFRGMKVPDVLMSGNHKKIDEWRHKESLRRTYTRRPDLLEGRELTKQEEIWLEQ
IKEEQ

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=244, Percent_Identity=45.0819672131148, Blast_Score=226, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 28130; Mature: 28130

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIDILTLFPEMFTGVFGSSILKKAQEKEAVELRVVNFRDYTTNKHNSVDDYPYGGGAG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCC
MVLTPQPIFDAVEDLTKETTQKPRIVLMCPQGERFTQKKAEELAKEEHVIFVCGHYEGYD
EEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCHH
ERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVLGNQNSQVEDSFSTGLLEHP
HHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
HYTRPADFRGMKVPDVLMSGNHKKIDEWRHKESLRRTYTRRPDLLEGRELTKQEEIWLEQ
CCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IKEEQ
HHCCC
>Mature Secondary Structure
MMKIDILTLFPEMFTGVFGSSILKKAQEKEAVELRVVNFRDYTTNKHNSVDDYPYGGGAG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCC
MVLTPQPIFDAVEDLTKETTQKPRIVLMCPQGERFTQKKAEELAKEEHVIFVCGHYEGYD
EEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCHH
ERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVLGNQNSQVEDSFSTGLLEHP
HHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
HYTRPADFRGMKVPDVLMSGNHKKIDEWRHKESLRRTYTRRPDLLEGRELTKQEEIWLEQ
CCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IKEEQ
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA