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The map label for this gene is topA [H]

Identifier: 152976199

GI number: 152976199

Start: 2583208

End: 2585286

Strand: Reverse

Name: topA [H]

Synonym: Bcer98_2485

Alternate gene names: 152976199

Gene position: 2585286-2583208 (Counterclockwise)

Preceding gene: 152976200

Following gene: 152976198

Centisome position: 63.26

GC content: 38.48

Gene sequence:

>2079_bases
ATGTCAGATTACCTTGTAATCGTGGAGTCGCCTTCTAAGGCGAAGACCATTGAAAAATATTTAGGAAAAAAATACAAAGT
TGTCGCGTCTATGGGACATGTACGCGATTTACCTAAAAGTCAAATGGGGATAGAGGTAAAAAATAACTTTACTCCAAAGT
ATATTACCATTCGTGGTAAGGGTCCTGTTTTAAAAGAGTTAAAATCAGCGGCAAAAAAAGCAAAGAAAGTTTATCTCGCA
GCCGATCCAGATCGCGAGGGAGAAGCGATTGCTTGGCATTTAGCGAACACGTTAAATGTTGATGTTGAGTCCGACTGCCG
AGTTGTGTTTAATGAAATTACGAAAGATGCAATAAAAGAGTCCTTTAAACATCCTCGTGCGATCAATATGGATTTAGTAG
ATGCACAGCAGGCGAGACGGATACTAGATCGTCTTGTTGGCTATAATATTAGTCCTTTATTATGGAAGAAAGTGAAAAAA
GGATTAAGTGCAGGGCGCGTACAATCTGTAGCGGTTCGCTTAATCATTGAGCGTGAACGAGAAATTCAAAACTTCGTTCC
TGAAGAGTTCTGGACAATTAAAACAGAATTTGTCAAAGGGAAAGAAACATTTGAAGCAGCTTTTTACGGTGTAAATGGTG
AAAAAGTTCAACTGACGAATGAAGCGCAAGTAAAAGATATAATTGAAAAAATAAAAGATAATGCGTTTTCAGTTGAAGAT
GTAACAAAGAAAGAACGAAAACGTAATCCAGCTTTACCGTTTACAACATCTTCATTACAGCAAGAAGCGGCACGCAAGTT
AAATATGCGTGCGAAGAAAACAATGATGCTTGCACAGCAACTATATGAAGGAATAGACATTGGAAAGCAAGGAACCGTAG
GTCTCATCACGTATATGAGAACAGATTCAACACGTATCTCTGAAACTGCGCAAGAAGAAGCTCGTTCTTACATCGCTGAT
GCATTTGGTTCAGAATACATAGGAACAGGGAAGAAAAAGGAAACAAAAAAAGCGAATGCACAAGATGCGCATGAAGCAAT
TCGTCCAACTTCTGTAATGAGAAAACCGGATGAATTGAAAAGTTTTCTAAGCCGAGATCAGTATCGTTTGTATAAATTAA
TTTGGGAGCGATTTGTTGCAAGTCAAATGGCATCTGCTATAATGGATACTGTTACAGCAAAGCTCATTAATAATGATGTT
CAGTTCCGGGCGAGTGGATCTGTAGTGAAATTCCCAGGTTTTATGAAAGTGTATGTGGAATCAAAAGATGATGGTGCGGA
AGAAAAAGATAAGATGTTGCCACCTTTAGAAGTGGGAGAAACGGTATTCTCAAAAGATGTAGAACCGAAGCAACATTTTA
CACAGCCGCCACCGCGTTATACAGAAGCGCGGCTCGTTCGCACACTTGAGGAACTTGGAATTGGAAGACCATCAACGTAT
GTGCCAACGCTTGAAACGATTCAAAAGCGCGGATATGTTGCACTAGACAATAAGCGATTTGTTCCTACTGAACTAGGTGA
AATTGTAACTGAACTTATGTTAGAGTTTTTTCCAGAAATTATTAATATCGAATTCACTGCCAATATGGAGCAAAATCTCG
ATGAAATAGAAGAAGGAAAAGCAAATTGGGTAACAATTGTCGATGATTTCTATACAGAATTTGCGCCGCGTTTAGAGAAA
GCGGAGAAGGAAATGCGTGAAATAGAGATTAAAGACGAACCAGCTGGGGAAGACTGTGAATTATGTGGTCACCCAATGGT
TTTTAAAATGGGTAGATATGGGAAGTTTATGGCTTGTTCGAATTTTCCAGATTGTCGTAATACAAAGCCGATTGTAAAAG
AAATCGGTGTAACTTGTCCGAAATGTGAAAAAGGACAAATTATTGAACGTCGAAGCAATAATAAGAAACGACTTTTCTAT
GGGTGTAGTACATATCCAGAATGTGACTTTGTATCCTGGGATAAGCCAATTGGTCGTAAATGTCCAAAATGTGAAGGAAT
GCTTATAGAGAAAAAGTTGAAAAAAGGCGTTCAGGTACAATGTATTTCGTGTGATTATGAAGAAGAACAACAAATGTGA

Upstream 100 bases:

>100_bases
AGATTGGCACTGTATGGTATACTCATTCTTGATTGACAAAAACAAGAGAAATCAATAAGATTAATGAAGACTTGAATCCA
CCTCTTAAGGAGGCACTAGC

Downstream 100 bases:

>100_bases
GCGTAACTGCTCACATTTTTTTCGGGAAAGAAAAGGAAAGGTGATATAGATATGACAACACAAGTGGTAAAGGTCATTGG
AGCGGGTCTTGCGGGAAGTG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSDYLVIVESPSKAKTIEKYLGKKYKVVASMGHVRDLPKSQMGIEVKNNFTPKYITIRGKGPVLKELKSAAKKAKKVYLA
ADPDREGEAIAWHLANTLNVDVESDCRVVFNEITKDAIKESFKHPRAINMDLVDAQQARRILDRLVGYNISPLLWKKVKK
GLSAGRVQSVAVRLIIEREREIQNFVPEEFWTIKTEFVKGKETFEAAFYGVNGEKVQLTNEAQVKDIIEKIKDNAFSVED
VTKKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQQLYEGIDIGKQGTVGLITYMRTDSTRISETAQEEARSYIAD
AFGSEYIGTGKKKETKKANAQDAHEAIRPTSVMRKPDELKSFLSRDQYRLYKLIWERFVASQMASAIMDTVTAKLINNDV
QFRASGSVVKFPGFMKVYVESKDDGAEEKDKMLPPLEVGETVFSKDVEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTY
VPTLETIQKRGYVALDNKRFVPTELGEIVTELMLEFFPEIINIEFTANMEQNLDEIEEGKANWVTIVDDFYTEFAPRLEK
AEKEMREIEIKDEPAGEDCELCGHPMVFKMGRYGKFMACSNFPDCRNTKPIVKEIGVTCPKCEKGQIIERRSNNKKRLFY
GCSTYPECDFVSWDKPIGRKCPKCEGMLIEKKLKKGVQVQCISCDYEEEQQM

Sequences:

>Translated_692_residues
MSDYLVIVESPSKAKTIEKYLGKKYKVVASMGHVRDLPKSQMGIEVKNNFTPKYITIRGKGPVLKELKSAAKKAKKVYLA
ADPDREGEAIAWHLANTLNVDVESDCRVVFNEITKDAIKESFKHPRAINMDLVDAQQARRILDRLVGYNISPLLWKKVKK
GLSAGRVQSVAVRLIIEREREIQNFVPEEFWTIKTEFVKGKETFEAAFYGVNGEKVQLTNEAQVKDIIEKIKDNAFSVED
VTKKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQQLYEGIDIGKQGTVGLITYMRTDSTRISETAQEEARSYIAD
AFGSEYIGTGKKKETKKANAQDAHEAIRPTSVMRKPDELKSFLSRDQYRLYKLIWERFVASQMASAIMDTVTAKLINNDV
QFRASGSVVKFPGFMKVYVESKDDGAEEKDKMLPPLEVGETVFSKDVEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTY
VPTLETIQKRGYVALDNKRFVPTELGEIVTELMLEFFPEIINIEFTANMEQNLDEIEEGKANWVTIVDDFYTEFAPRLEK
AEKEMREIEIKDEPAGEDCELCGHPMVFKMGRYGKFMACSNFPDCRNTKPIVKEIGVTCPKCEKGQIIERRSNNKKRLFY
GCSTYPECDFVSWDKPIGRKCPKCEGMLIEKKLKKGVQVQCISCDYEEEQQM
>Mature_691_residues
SDYLVIVESPSKAKTIEKYLGKKYKVVASMGHVRDLPKSQMGIEVKNNFTPKYITIRGKGPVLKELKSAAKKAKKVYLAA
DPDREGEAIAWHLANTLNVDVESDCRVVFNEITKDAIKESFKHPRAINMDLVDAQQARRILDRLVGYNISPLLWKKVKKG
LSAGRVQSVAVRLIIEREREIQNFVPEEFWTIKTEFVKGKETFEAAFYGVNGEKVQLTNEAQVKDIIEKIKDNAFSVEDV
TKKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQQLYEGIDIGKQGTVGLITYMRTDSTRISETAQEEARSYIADA
FGSEYIGTGKKKETKKANAQDAHEAIRPTSVMRKPDELKSFLSRDQYRLYKLIWERFVASQMASAIMDTVTAKLINNDVQ
FRASGSVVKFPGFMKVYVESKDDGAEEKDKMLPPLEVGETVFSKDVEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTYV
PTLETIQKRGYVALDNKRFVPTELGEIVTELMLEFFPEIINIEFTANMEQNLDEIEEGKANWVTIVDDFYTEFAPRLEKA
EKEMREIEIKDEPAGEDCELCGHPMVFKMGRYGKFMACSNFPDCRNTKPIVKEIGVTCPKCEKGQIIERRSNNKKRLFYG
CSTYPECDFVSWDKPIGRKCPKCEGMLIEKKLKKGVQVQCISCDYEEEQQM

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=609, Percent_Identity=24.7947454844007, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI4507635, Length=518, Percent_Identity=24.7104247104247, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1787529, Length=754, Percent_Identity=39.5225464190981, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1788061, Length=604, Percent_Identity=22.5165562913907, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI48994931, Length=111, Percent_Identity=43.2432432432432, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI32563869, Length=562, Percent_Identity=25.0889679715303, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17555378, Length=509, Percent_Identity=24.5579567779961, Blast_Score=124, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=553, Percent_Identity=25.3164556962025, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585251, Length=626, Percent_Identity=23.961661341853, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24640096, Length=507, Percent_Identity=25.6410256410256, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78986; Mature: 78855

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYLVIVESPSKAKTIEKYLGKKYKVVASMGHVRDLPKSQMGIEVKNNFTPKYITIRGK
CCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCEEEECCCCCCEEEEECC
GPVLKELKSAAKKAKKVYLAADPDREGEAIAWHLANTLNVDVESDCRVVFNEITKDAIKE
CHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHH
SFKHPRAINMDLVDAQQARRILDRLVGYNISPLLWKKVKKGLSAGRVQSVAVRLIIERER
HHCCCCCCCCHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EIQNFVPEEFWTIKTEFVKGKETFEAAFYGVNGEKVQLTNEAQVKDIIEKIKDNAFSVED
HHHHCCCHHHHHHHHHHHCCHHHHHHHEECCCCCEEEECCHHHHHHHHHHHHCCCCCHHH
VTKKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQQLYEGIDIGKQGTVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
TDSTRISETAQEEARSYIADAFGSEYIGTGKKKETKKANAQDAHEAIRPTSVMRKPDELK
CCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHCCCHHHHHHHHCCHHHHCCHHHHH
SFLSRDQYRLYKLIWERFVASQMASAIMDTVTAKLINNDVQFRASGSVVKFPGFMKVYVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCEEEEEEE
SKDDGAEEKDKMLPPLEVGETVFSKDVEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTY
CCCCCCCHHHHCCCCHHHCHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VPTLETIQKRGYVALDNKRFVPTELGEIVTELMLEFFPEIINIEFTANMEQNLDEIEEGK
CCHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHCCC
ANWVTIVDDFYTEFAPRLEKAEKEMREIEIKDEPAGEDCELCGHPMVFKMGRYGKFMACS
CCEEEEHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCCEEECCCCCCEEEEC
NFPDCRNTKPIVKEIGVTCPKCEKGQIIERRSNNKKRLFYGCSTYPECDFVSWDKPIGRK
CCCCCCCCCHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
CPKCEGMLIEKKLKKGVQVQCISCDYEEEQQM
CCCCCCHHHHHHHHCCCEEEEEECCCHHHHCC
>Mature Secondary Structure 
SDYLVIVESPSKAKTIEKYLGKKYKVVASMGHVRDLPKSQMGIEVKNNFTPKYITIRGK
CCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCEEEECCCCCCEEEEECC
GPVLKELKSAAKKAKKVYLAADPDREGEAIAWHLANTLNVDVESDCRVVFNEITKDAIKE
CHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHH
SFKHPRAINMDLVDAQQARRILDRLVGYNISPLLWKKVKKGLSAGRVQSVAVRLIIERER
HHCCCCCCCCHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EIQNFVPEEFWTIKTEFVKGKETFEAAFYGVNGEKVQLTNEAQVKDIIEKIKDNAFSVED
HHHHCCCHHHHHHHHHHHCCHHHHHHHEECCCCCEEEECCHHHHHHHHHHHHCCCCCHHH
VTKKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQQLYEGIDIGKQGTVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
TDSTRISETAQEEARSYIADAFGSEYIGTGKKKETKKANAQDAHEAIRPTSVMRKPDELK
CCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHCCCHHHHHHHHCCHHHHCCHHHHH
SFLSRDQYRLYKLIWERFVASQMASAIMDTVTAKLINNDVQFRASGSVVKFPGFMKVYVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCEEEEEEE
SKDDGAEEKDKMLPPLEVGETVFSKDVEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTY
CCCCCCCHHHHCCCCHHHCHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VPTLETIQKRGYVALDNKRFVPTELGEIVTELMLEFFPEIINIEFTANMEQNLDEIEEGK
CCHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHCCC
ANWVTIVDDFYTEFAPRLEKAEKEMREIEIKDEPAGEDCELCGHPMVFKMGRYGKFMACS
CCEEEEHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCCEEECCCCCCEEEEC
NFPDCRNTKPIVKEIGVTCPKCEKGQIIERRSNNKKRLFYGCSTYPECDFVSWDKPIGRK
CCCCCCCCCHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
CPKCEGMLIEKKLKKGVQVQCISCDYEEEQQM
CCCCCCHHHHHHHHCCCEEEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]