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The map label for this gene is trmFO [H]

Identifier: 152976198

GI number: 152976198

Start: 2581852

End: 2583156

Strand: Reverse

Name: trmFO [H]

Synonym: Bcer98_2484

Alternate gene names: 152976198

Gene position: 2583156-2581852 (Counterclockwise)

Preceding gene: 152976199

Following gene: 152976197

Centisome position: 63.2

GC content: 39.08

Gene sequence:

>1305_bases
ATGACAACACAAGTGGTAAAGGTCATTGGAGCGGGTCTTGCGGGAAGTGAAGCAGCTTATCAAATTGCAAAACGTGGTAT
CCAAGTCCATTTATATGAAATGAGACCTGTAAAGCAAACGCCAGCTCACCATACAGACAAATTCGCTGAATTAGTATGTA
GTAATTCACTTCGTGCGAATACATTAACAAACGCAGTGGGAGTTATTAAAGAGGAAATGCGTCGAATGGATTCTGTCATT
ATTCGCGCAGCGGATGAATGTTCTGTACCAGCAGGGGGAGCATTAGCTGTAGACCGTCACGAGTTTGCAGCAAAGGTAAC
AGAATATGTGAAGAATCACCCAAACGTAACTGTAATGAATGAAGAGATTACAGAAATACCAGAAGGGCCAACAGTTATTG
CGACAGGTCCACTTACCTCTCCAGCTCTTGCAGCGCAATTAAAAGAGTTGACGGGTGAAGAGTATTTCTATTTCTATGAT
GCAGCTGCACCAATTATAGAAAAAGACAGCATTGATATGAATAAAGTATATTTAAAATCTCGATATGATAAAGGAGAAGC
GGCGTATTTAAACTGTCCAATGACAGAAGATGAATTCAACCGTTTCTATGATGCGTTAATAGCCGCTGAGACAGTACCAT
TAAAAGAGTTTGAAAAAGAAATTTTCTTTGAAGGATGTATGCCAGTTGAAGTAATGGCAGCAAGAGGAAGACAAACATTA
TTATTCGGACCGATGAAACCGGTTGGATTAGAAGATCCAAAGACAGGTAAAACACCATATGCGGTTGTTCAATTACGTCA
AGATGATGCAGCGGGAACACTATATAATATCGTAGGTTTTCAAACGCATTTAAAATGGGGACCGCAAAAAGAGGTATTAC
AGTTAATTCCAGGTCTTGAAAACGCAGAAATTGTACGTTATGGCGTTATGCATCGCAATACATTTATTAATTCGCCAAAG
TTACTTCGTCCAACGTATCAATATAAACATCGTGATGATTTATTCTTTGCTGGTCAAATGACTGGGGTAGAGGGATACGT
AGAGTCTGCAGCTTCAGGTTTATTAGCTGGTATTAATGCAGCTCGTCTTCTTAAAGGAGAAGAACCTGTTGTATTACCAT
CTGTGACGGCAATGGGAAGTATGGCAAATTACATTACTTCGACAAACGCGAAAAATTTCCAACCAATGAATGCCAACTTC
GGTTTATTTGCACCGCTAGAAAAGAAAATTAAAAAGAAACAAGAGCGCAATGAAGCATACGCAACACGTGCTTTAGAAAC
AATTCAAAATTTTGTAAATATTTAG

Upstream 100 bases:

>100_bases
TCAGGTACAATGTATTTCGTGTGATTATGAAGAAGAACAACAAATGTGAGCGTAACTGCTCACATTTTTTTCGGGAAAGA
AAAGGAAAGGTGATATAGAT

Downstream 100 bases:

>100_bases
TTAAATTTCTTGCAAAGGCTACTGAGTTGTGATACGATTTAGTAGCCTTTATTGGGGTGATTTATGTGAATGTGAAGAAA
TTGTTACAATTATTCGTTGG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTTQVVKVIGAGLAGSEAAYQIAKRGIQVHLYEMRPVKQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRRMDSVI
IRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITEIPEGPTVIATGPLTSPALAAQLKELTGEEYFYFYD
AAAPIIEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDALIAAETVPLKEFEKEIFFEGCMPVEVMAARGRQTL
LFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPK
LLRPTYQYKHRDDLFFAGQMTGVEGYVESAASGLLAGINAARLLKGEEPVVLPSVTAMGSMANYITSTNAKNFQPMNANF
GLFAPLEKKIKKKQERNEAYATRALETIQNFVNI

Sequences:

>Translated_434_residues
MTTQVVKVIGAGLAGSEAAYQIAKRGIQVHLYEMRPVKQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRRMDSVI
IRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITEIPEGPTVIATGPLTSPALAAQLKELTGEEYFYFYD
AAAPIIEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDALIAAETVPLKEFEKEIFFEGCMPVEVMAARGRQTL
LFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPK
LLRPTYQYKHRDDLFFAGQMTGVEGYVESAASGLLAGINAARLLKGEEPVVLPSVTAMGSMANYITSTNAKNFQPMNANF
GLFAPLEKKIKKKQERNEAYATRALETIQNFVNI
>Mature_433_residues
TTQVVKVIGAGLAGSEAAYQIAKRGIQVHLYEMRPVKQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRRMDSVII
RAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITEIPEGPTVIATGPLTSPALAAQLKELTGEEYFYFYDA
AAPIIEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDALIAAETVPLKEFEKEIFFEGCMPVEVMAARGRQTLL
FGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPKL
LRPTYQYKHRDDLFFAGQMTGVEGYVESAASGLLAGINAARLLKGEEPVVLPSVTAMGSMANYITSTNAKNFQPMNANFG
LFAPLEKKIKKKQERNEAYATRALETIQNFVNI

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=45.5555555555556, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17534255, Length=131, Percent_Identity=35.1145038167939, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321202, Length=170, Percent_Identity=30, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 48058; Mature: 47926

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQVVKVIGAGLAGSEAAYQIAKRGIQVHLYEMRPVKQTPAHHTDKFAELVCSNSLRAN
CCHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHH
TLTNAVGVIKEEMRRMDSVIIRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMN
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEC
EEITEIPEGPTVIATGPLTSPALAAQLKELTGEEYFYFYDAAAPIIEKDSIDMNKVYLKS
CHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCHHHHHHHH
RYDKGEAAYLNCPMTEDEFNRFYDALIAAETVPLKEFEKEIFFEGCMPVEVMAARGRQTL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
LFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
NAEIVRYGVMHRNTFINSPKLLRPTYQYKHRDDLFFAGQMTGVEGYVESAASGLLAGINA
CCCEEEEEHHHCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCH
ARLLKGEEPVVLPSVTAMGSMANYITSTNAKNFQPMNANFGLFAPLEKKIKKKQERNEAY
HHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHH
ATRALETIQNFVNI
HHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQVVKVIGAGLAGSEAAYQIAKRGIQVHLYEMRPVKQTPAHHTDKFAELVCSNSLRAN
CHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHH
TLTNAVGVIKEEMRRMDSVIIRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMN
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEC
EEITEIPEGPTVIATGPLTSPALAAQLKELTGEEYFYFYDAAAPIIEKDSIDMNKVYLKS
CHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCHHHHHHHH
RYDKGEAAYLNCPMTEDEFNRFYDALIAAETVPLKEFEKEIFFEGCMPVEVMAARGRQTL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
LFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
NAEIVRYGVMHRNTFINSPKLLRPTYQYKHRDDLFFAGQMTGVEGYVESAASGLLAGINA
CCCEEEEEHHHCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCH
ARLLKGEEPVVLPSVTAMGSMANYITSTNAKNFQPMNANFGLFAPLEKKIKKKQERNEAY
HHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHH
ATRALETIQNFVNI
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA