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The map label for this gene is miaB [H]

Identifier: 152976138

GI number: 152976138

Start: 2514204

End: 2515733

Strand: Reverse

Name: miaB [H]

Synonym: Bcer98_2424

Alternate gene names: 152976138

Gene position: 2515733-2514204 (Counterclockwise)

Preceding gene: 152976139

Following gene: 152976137

Centisome position: 61.55

GC content: 36.21

Gene sequence:

>1530_bases
ATGAACGAGCAACAACGATTAGCAAGTCAACAAGCAAATGCTTCTACGAAGAAGGAAGAAAAAGATTATAGTAAGTACTT
TGAACATGTTTATCAGCCACCTTCATTAAAAGATGCGAAAAAACGTGGGAAAGAACAAGTGAAAATCGAGCGTGATTTTG
GTCTTCCAGAAGAATTTCGTAATTTTGGGAAAGGAAGAAAGTTTTATATCCGTACGTATGGATGTCAAATGAACGAGCAT
GATACAGAAGTAATGGCTGGTATTTTTACAACGCTTGGATATGAACCGACATTTACAACAGAAGATGCAGATGTCATTTT
ATTAAATACTTGTGCGATTCGTGAGAACGCTGAGAATAAGGTGTTCGGAGAACTAGGTCACTTAAAACCATTGAAACAAA
AGAATCCAGATCTGTTAATTGGTGTATGTGGTTGTATGTCGCAAGAAGAATCTGTTGTAAACAAAATTATGCAAAAGCAT
CAACATGTAGATATGGTATTTGGTACGCATAATATTCATCGATTACCGTATATTTTGAAAGATGCAATGTTCTCTAAAGC
GACAGTTGTTGAAGTTTGGTCTAAAGAAGGAGACGTAATCGAAAATCTTCCAAAAGTACGTCGCGGTGATATTAAAGCTT
GGGTAAACATTATGTATGGATGTGATAAATTCTGTACATATTGTATCGTACCGTATACACGCGGGAAAGAGCGAAGCCGT
CGCCCAGAAGATATTATTAAAGAAGTTCGCCATTTAGCAGCAAATGGCTATAAAGAAATTACATTGCTTGGCCAAAACGT
AAATGCATACGGAAAAGACTTTGATGATCTAGAATATGGTCTTGGCGATTTAATGGATGAACTTCGTAAGATTGATATTG
CACGTATTCGTTTTACAACAAGTCATCCACGTGACTTTGACGATCACTTAATTGAAGTGCTTGGAAAAGGTGGTAACTTA
GTAGAGCATATTCACTTACCAGTACAATCTGGTAGCACGGATATGTTGAAGATTATGGCGCGTAAATATACACGTGAGCA
GTACTTAGAACTTGTACGTAAAATTAAAAAGACAATTCCTAATGTAGTATTAACAACGGATATTATCGTTGGTTTCCCAA
ATGAAACAGACGAACAATTTGAAGAAACATTATCTTTATATCGTGAAGTAGAATTTGATAGTGCGTTCACATTTATTTAC
TCTCCACGTGAAGGTACGCCAGCTGCGAAAATGAAAGACAATATACCAATGGAAGTGAAAAAAGAACGTCTACAGCGTTT
GAATGAACTTGTAAATGAATTCTCTGCGAAGAAAAATAAGAAATATGAAGGACAAATTGTTGAAGTATTAGTTGATGGAG
AAAGTAAAAATAATCCAGACGTACTTGCAGGCTACACGCGTACGAATAAACTTGTAAACTTCGTTGCACCAAAATCTGTA
ATCGGTCAGCTTGTAAAAGTAAAAATTACTGAAGCGAAAACTTGGTCTCTAAACGGGGAATTAGTTGAGGAGCCGATTGA
GGTGAAATAA

Upstream 100 bases:

>100_bases
GTTTCAAAGGGGGAGCTATTTACTATATATCCTAGTCCTTAAGATGAATCTAGAATAGGCTACAAGTGTGTAGAGTTTTA
AGGTTGAAAGGAGATTACCC

Downstream 100 bases:

>100_bases
TGAAAGTCTATACAAAGGATGAAATTGTTGAGCAAGCGAAAGAGTTAGCAAAAATGATTTCTGAAACAGAAGAAGTAGAT
TTCTTCAAGCGCGCAGAAGC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNEQQRLASQQANASTKKEEKDYSKYFEHVYQPPSLKDAKKRGKEQVKIERDFGLPEEFRNFGKGRKFYIRTYGCQMNEH
DTEVMAGIFTTLGYEPTFTTEDADVILLNTCAIRENAENKVFGELGHLKPLKQKNPDLLIGVCGCMSQEESVVNKIMQKH
QHVDMVFGTHNIHRLPYILKDAMFSKATVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIKEVRHLAANGYKEITLLGQNVNAYGKDFDDLEYGLGDLMDELRKIDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTDMLKIMARKYTREQYLELVRKIKKTIPNVVLTTDIIVGFPNETDEQFEETLSLYREVEFDSAFTFIY
SPREGTPAAKMKDNIPMEVKKERLQRLNELVNEFSAKKNKKYEGQIVEVLVDGESKNNPDVLAGYTRTNKLVNFVAPKSV
IGQLVKVKITEAKTWSLNGELVEEPIEVK

Sequences:

>Translated_509_residues
MNEQQRLASQQANASTKKEEKDYSKYFEHVYQPPSLKDAKKRGKEQVKIERDFGLPEEFRNFGKGRKFYIRTYGCQMNEH
DTEVMAGIFTTLGYEPTFTTEDADVILLNTCAIRENAENKVFGELGHLKPLKQKNPDLLIGVCGCMSQEESVVNKIMQKH
QHVDMVFGTHNIHRLPYILKDAMFSKATVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIKEVRHLAANGYKEITLLGQNVNAYGKDFDDLEYGLGDLMDELRKIDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTDMLKIMARKYTREQYLELVRKIKKTIPNVVLTTDIIVGFPNETDEQFEETLSLYREVEFDSAFTFIY
SPREGTPAAKMKDNIPMEVKKERLQRLNELVNEFSAKKNKKYEGQIVEVLVDGESKNNPDVLAGYTRTNKLVNFVAPKSV
IGQLVKVKITEAKTWSLNGELVEEPIEVK
>Mature_509_residues
MNEQQRLASQQANASTKKEEKDYSKYFEHVYQPPSLKDAKKRGKEQVKIERDFGLPEEFRNFGKGRKFYIRTYGCQMNEH
DTEVMAGIFTTLGYEPTFTTEDADVILLNTCAIRENAENKVFGELGHLKPLKQKNPDLLIGVCGCMSQEESVVNKIMQKH
QHVDMVFGTHNIHRLPYILKDAMFSKATVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIKEVRHLAANGYKEITLLGQNVNAYGKDFDDLEYGLGDLMDELRKIDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTDMLKIMARKYTREQYLELVRKIKKTIPNVVLTTDIIVGFPNETDEQFEETLSLYREVEFDSAFTFIY
SPREGTPAAKMKDNIPMEVKKERLQRLNELVNEFSAKKNKKYEGQIVEVLVDGESKNNPDVLAGYTRTNKLVNFVAPKSV
IGQLVKVKITEAKTWSLNGELVEEPIEVK

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=37.2670807453416, Blast_Score=308, Evalue=1e-83,
Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=37.2670807453416, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI93277076, Length=455, Percent_Identity=29.2307692307692, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=44.7727272727273, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=26.8722466960352, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=35.4166666666667, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI71996771, Length=364, Percent_Identity=28.5714285714286, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21356207, Length=544, Percent_Identity=34.7426470588235, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI19922432, Length=458, Percent_Identity=30.1310043668122, Blast_Score=194, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 58493; Mature: 58493

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQQRLASQQANASTKKEEKDYSKYFEHVYQPPSLKDAKKRGKEQVKIERDFGLPEEFR
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEEEECCCCCCHHHH
NFGKGRKFYIRTYGCQMNEHDTEVMAGIFTTLGYEPTFTTEDADVILLNTCAIRENAENK
HHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCH
VFGELGHLKPLKQKNPDLLIGVCGCMSQEESVVNKIMQKHQHVDMVFGTHNIHRLPYILK
HHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHH
DAMFSKATVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCCHHHHHHHCCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIKEVRHLAANGYKEITLLGQNVNAYGKDFDDLEYGLGDLMDELRKIDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYTREQYLELVRKIKKTIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVVLTTDIIVGFPNETDEQFEETLSLYREVEFDSAFTFIYSPREGTPAAKMKDNIPMEVK
CEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNELVNEFSAKKNKKYEGQIVEVLVDGESKNNPDVLAGYTRTNKLVNFVAPKSV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEHHHHHHHHHCCCHHH
IGQLVKVKITEAKTWSLNGELVEEPIEVK
HHHHHEEEEECCEEEECCCHHHCCCCCCC
>Mature Secondary Structure
MNEQQRLASQQANASTKKEEKDYSKYFEHVYQPPSLKDAKKRGKEQVKIERDFGLPEEFR
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEEEECCCCCCHHHH
NFGKGRKFYIRTYGCQMNEHDTEVMAGIFTTLGYEPTFTTEDADVILLNTCAIRENAENK
HHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCH
VFGELGHLKPLKQKNPDLLIGVCGCMSQEESVVNKIMQKHQHVDMVFGTHNIHRLPYILK
HHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHH
DAMFSKATVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCCHHHHHHHCCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIKEVRHLAANGYKEITLLGQNVNAYGKDFDDLEYGLGDLMDELRKIDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYTREQYLELVRKIKKTIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVVLTTDIIVGFPNETDEQFEETLSLYREVEFDSAFTFIYSPREGTPAAKMKDNIPMEVK
CEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNELVNEFSAKKNKKYEGQIVEVLVDGESKNNPDVLAGYTRTNKLVNFVAPKSV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEHHHHHHHHHCCCHHH
IGQLVKVKITEAKTWSLNGELVEEPIEVK
HHHHHEEEEECCEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA