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The map label for this gene is cobB [H]

Identifier: 152975801

GI number: 152975801

Start: 2159457

End: 2160173

Strand: Reverse

Name: cobB [H]

Synonym: Bcer98_2055

Alternate gene names: 152975801

Gene position: 2160173-2159457 (Counterclockwise)

Preceding gene: 152975802

Following gene: 152975796

Centisome position: 52.85

GC content: 34.31

Gene sequence:

>717_bases
ATGCAACAATACGAAGAAGTTCGCTCCATTCTAGAAAATGCAAAGAAAATAACGGTATTAACTGGCGCAGGTGCAAGTAC
AGAAAGTGGAATACCTGATTTTCGCTCAGCAAATGGATTATATGCGGATGCAAAAGTAGAAATGTATTTATCGAGGGGAT
ATTATAATCGCGATCCGAAAGAATTTTGGAAACATTATAAAGAGATTTTTCAAATTCGTACATTTCATCAATATAAGCCG
AATCGTGGACATATATTTTTAGCAAAGTTAGAAGAAGAAGGAAAAGATGTGACTATATTAACACAAAATATTGATGGCTT
ACACCAATTAGGTGGTAGTAAACATGTAATCGATTTACATGGAACATTGCAAACAGCGCATTGTCCGAAATGTAAAACGA
AATACGATTTACAATATATGATTCATCATGAAGTACCGCGCTGTGAAATGTGTAATTTTATTTTAAATCCAGATGTTGTT
TTATATGGCGATACACTGCCACAATATCAAAATGCACTTCAACGTTTATATGAAACGGATGTATTATTAGTTATGGGAAC
GTCCTTAAAAGTACAACCAGTTGCATCATTTCCGCAAATTGCAAAGAGAGAAGTAGGAGCAACAACCATTTTAGTAAACG
AGGAATTAACGGGGCAAGAACATCATTTCGATTATGTATTTCAGCTTAAGATTGGTGACTTTGCAAAGAGAATGTAA

Upstream 100 bases:

>100_bases
TTACAAGTGGAAAAAGATATTGTAGAGTGTGGGGGAACTACACATTAAGACAATGATAACTGGTATAATGTAGAAAGAAA
TCTACATAGAGGTGAGAGTT

Downstream 100 bases:

>100_bases
AAAATTCACGCACTCCTTTTTCAAAAGCAAAATTAATAGACGAAGTGAAAACAGTAGAATATCTCATCTATCTTTCTAGC
GAAATCTTACGCCGATGAAT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MQQYEEVRSILENAKKITVLTGAGASTESGIPDFRSANGLYADAKVEMYLSRGYYNRDPKEFWKHYKEIFQIRTFHQYKP
NRGHIFLAKLEEEGKDVTILTQNIDGLHQLGGSKHVIDLHGTLQTAHCPKCKTKYDLQYMIHHEVPRCEMCNFILNPDVV
LYGDTLPQYQNALQRLYETDVLLVMGTSLKVQPVASFPQIAKREVGATTILVNEELTGQEHHFDYVFQLKIGDFAKRM

Sequences:

>Translated_238_residues
MQQYEEVRSILENAKKITVLTGAGASTESGIPDFRSANGLYADAKVEMYLSRGYYNRDPKEFWKHYKEIFQIRTFHQYKP
NRGHIFLAKLEEEGKDVTILTQNIDGLHQLGGSKHVIDLHGTLQTAHCPKCKTKYDLQYMIHHEVPRCEMCNFILNPDVV
LYGDTLPQYQNALQRLYETDVLLVMGTSLKVQPVASFPQIAKREVGATTILVNEELTGQEHHFDYVFQLKIGDFAKRM
>Mature_238_residues
MQQYEEVRSILENAKKITVLTGAGASTESGIPDFRSANGLYADAKVEMYLSRGYYNRDPKEFWKHYKEIFQIRTFHQYKP
NRGHIFLAKLEEEGKDVTILTQNIDGLHQLGGSKHVIDLHGTLQTAHCPKCKTKYDLQYMIHHEVPRCEMCNFILNPDVV
LYGDTLPQYQNALQRLYETDVLLVMGTSLKVQPVASFPQIAKREVGATTILVNEELTGQEHHFDYVFQLKIGDFAKRM

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI7657575, Length=239, Percent_Identity=38.4937238493724, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI13775602, Length=229, Percent_Identity=36.2445414847162, Blast_Score=137, Evalue=6e-33,
Organism=Homo sapiens, GI13775600, Length=229, Percent_Identity=36.2445414847162, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI6912660, Length=218, Percent_Identity=34.4036697247706, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI63054862, Length=213, Percent_Identity=34.7417840375587, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI300797705, Length=201, Percent_Identity=35.3233830845771, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI300795542, Length=244, Percent_Identity=30.7377049180328, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI6912664, Length=258, Percent_Identity=30.2325581395349, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI215982798, Length=182, Percent_Identity=37.9120879120879, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI13787215, Length=251, Percent_Identity=29.8804780876494, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI6912662, Length=226, Percent_Identity=30.5309734513274, Blast_Score=98, Evalue=5e-21,
Organism=Homo sapiens, GI300797577, Length=236, Percent_Identity=27.9661016949153, Blast_Score=84, Evalue=8e-17,
Organism=Escherichia coli, GI308199517, Length=203, Percent_Identity=34.4827586206897, Blast_Score=104, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17541892, Length=221, Percent_Identity=33.9366515837104, Blast_Score=128, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71990482, Length=270, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17567771, Length=226, Percent_Identity=27.8761061946903, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71990487, Length=272, Percent_Identity=27.5735294117647, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324599, Length=273, Percent_Identity=29.3040293040293, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324504, Length=253, Percent_Identity=28.8537549407115, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325242, Length=234, Percent_Identity=29.9145299145299, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320163, Length=254, Percent_Identity=27.9527559055118, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320397, Length=235, Percent_Identity=25.9574468085106, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137536, Length=271, Percent_Identity=32.8413284132841, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24648389, Length=220, Percent_Identity=33.1818181818182, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28571445, Length=194, Percent_Identity=29.8969072164948, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24645650, Length=245, Percent_Identity=22.8571428571429, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27370; Mature: 27370

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYEEVRSILENAKKITVLTGAGASTESGIPDFRSANGLYADAKVEMYLSRGYYNRDPK
CCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCEEEEEEECCCCCCCHH
EFWKHYKEIFQIRTFHQYKPNRGHIFLAKLEEEGKDVTILTQNIDGLHQLGGSKHVIDLH
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHCCCCCEEEEEC
GTLQTAHCPKCKTKYDLQYMIHHEVPRCEMCNFILNPDVVLYGDTLPQYQNALQRLYETD
CCEECCCCCCCCCCCCEEEEEECCCCHHHHHHEEECCCEEEECCCCHHHHHHHHHHHHCC
VLLVMGTSLKVQPVASFPQIAKREVGATTILVNEELTGQEHHFDYVFQLKIGDFAKRM
EEEEECCCEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEECHHHHCC
>Mature Secondary Structure
MQQYEEVRSILENAKKITVLTGAGASTESGIPDFRSANGLYADAKVEMYLSRGYYNRDPK
CCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCEEEEEEECCCCCCCHH
EFWKHYKEIFQIRTFHQYKPNRGHIFLAKLEEEGKDVTILTQNIDGLHQLGGSKHVIDLH
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHCCCCCEEEEEC
GTLQTAHCPKCKTKYDLQYMIHHEVPRCEMCNFILNPDVVLYGDTLPQYQNALQRLYETD
CCEECCCCCCCCCCCCEEEEEECCCCHHHHHHEEECCCEEEECCCCHHHHHHHHHHHHCC
VLLVMGTSLKVQPVASFPQIAKREVGATTILVNEELTGQEHHFDYVFQLKIGDFAKRM
EEEEECCCEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]