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The map label for this gene is ywpJ [H]

Identifier: 152975647

GI number: 152975647

Start: 1996008

End: 1996862

Strand: Reverse

Name: ywpJ [H]

Synonym: Bcer98_1881

Alternate gene names: 152975647

Gene position: 1996862-1996008 (Counterclockwise)

Preceding gene: 152975648

Following gene: 152975646

Centisome position: 48.86

GC content: 32.63

Gene sequence:

>855_bases
ATGAAACTTATTGCGATTGATTTAGATGGAACGCTTCTTTCGAAAAATAAAATGATTAGTAAAGAAAATGCAGAAGCGAT
TCGAAAATGTCAAGAAGAAGGGCATGTTGTAGCTATTTGTACCGGACGTTCTATCGTTGACATTGAACGACTGTTACAAG
AAGTAAACTTACAATGCCCAATTATCGCAGAAAATGGTGCAGTTATTTATAAAGATAAAACAATGTTGAAAAGATACGCT
ATACAAAATGAACACGCACTTGACATTGTAGAATATTTGGAGGAGCAAGGTATGTATTATCAATTATATACGGATAAAGG
TGTATACGTACCAAAGTATGGTGAAGAAAATATACGTAAAGAAATGGAAGTGATTCGGAATCTAGATATAAACATTAGTG
AAGAAGAGGTGGAAAACATTGCAGCACTTTACCTTGAACATACAGCGTTTCATAGTATAGAGAGTTGTAAGTCGATTATA
AATAAAGAATTATATGTGCATAAATTTTTACCTTTCTCTTATGATCAACAGAAATTAAAAAGGTTAAAAGAAAAGTTCCA
GCATAACAGCGAGTTAATTATTTCTGCTTCTTATTGGCATAATTTAGAGATTAATCACTGTGATGCTCAAAAGGGAAATG
GATTATATACTTTAGCGGAATATTTTAATATTCCATTTGAAAACACAGTAGCCATTGGTGATGGACTAAATGATGTTTCC
ATGATGCAAAAAGCCAACGTATCCATTGCAATGGGGAATGCTGTTAATGAGTTAAAAGCAATGTGTCACTATGAAACATT
AACAAATGAAGAGCACGGTGTTGCACATGCTTTATACCATTATGTAATAGCATAA

Upstream 100 bases:

>100_bases
CGCTTTTCATAAATTATAACTGAGAGGTAGTTACTGCGTTAGATATGGAAACGATATCTCCCTATCTTTTCGCTGATTCC
TAGAAAGTGGTGAAGAAATA

Downstream 100 bases:

>100_bases
AAGGATCATAATTGGAACAAAAAAGAATCCGAATTCGGATTCTTTTTTGTTTTTTATTAGGGGTAAGGTATTCTATGAGA
CTTATAGAATCCTTATGACA

Product: Cof-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKLIAIDLDGTLLSKNKMISKENAEAIRKCQEEGHVVAICTGRSIVDIERLLQEVNLQCPIIAENGAVIYKDKTMLKRYA
IQNEHALDIVEYLEEQGMYYQLYTDKGVYVPKYGEENIRKEMEVIRNLDINISEEEVENIAALYLEHTAFHSIESCKSII
NKELYVHKFLPFSYDQQKLKRLKEKFQHNSELIISASYWHNLEINHCDAQKGNGLYTLAEYFNIPFENTVAIGDGLNDVS
MMQKANVSIAMGNAVNELKAMCHYETLTNEEHGVAHALYHYVIA

Sequences:

>Translated_284_residues
MKLIAIDLDGTLLSKNKMISKENAEAIRKCQEEGHVVAICTGRSIVDIERLLQEVNLQCPIIAENGAVIYKDKTMLKRYA
IQNEHALDIVEYLEEQGMYYQLYTDKGVYVPKYGEENIRKEMEVIRNLDINISEEEVENIAALYLEHTAFHSIESCKSII
NKELYVHKFLPFSYDQQKLKRLKEKFQHNSELIISASYWHNLEINHCDAQKGNGLYTLAEYFNIPFENTVAIGDGLNDVS
MMQKANVSIAMGNAVNELKAMCHYETLTNEEHGVAHALYHYVIA
>Mature_284_residues
MKLIAIDLDGTLLSKNKMISKENAEAIRKCQEEGHVVAICTGRSIVDIERLLQEVNLQCPIIAENGAVIYKDKTMLKRYA
IQNEHALDIVEYLEEQGMYYQLYTDKGVYVPKYGEENIRKEMEVIRNLDINISEEEVENIAALYLEHTAFHSIESCKSII
NKELYVHKFLPFSYDQQKLKRLKEKFQHNSELIISASYWHNLEINHCDAQKGNGLYTLAEYFNIPFENTVAIGDGLNDVS
MMQKANVSIAMGNAVNELKAMCHYETLTNEEHGVAHALYHYVIA

Specific function: Putative phosphatase (Potential) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI1786982, Length=287, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI2367265, Length=292, Percent_Identity=28.0821917808219, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787043, Length=278, Percent_Identity=23.7410071942446, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081790, Length=282, Percent_Identity=22.3404255319149, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 32585; Mature: 32585

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIAIDLDGTLLSKNKMISKENAEAIRKCQEEGHVVAICTGRSIVDIERLLQEVNLQCP
CEEEEEECCCEEECCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCE
IIAENGAVIYKDKTMLKRYAIQNEHALDIVEYLEEQGMYYQLYTDKGVYVPKYGEENIRK
EEECCCEEEEECHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCEECCCCCHHHHHH
EMEVIRNLDINISEEEVENIAALYLEHTAFHSIESCKSIINKELYVHKFLPFSYDQQKLK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHH
RLKEKFQHNSELIISASYWHNLEINHCDAQKGNGLYTLAEYFNIPFENTVAIGDGLNDVS
HHHHHHCCCCEEEEEEECEECEEEEECCCCCCCCEEEEHHHHCCCCCCEEEECCCCCHHH
MMQKANVSIAMGNAVNELKAMCHYETLTNEEHGVAHALYHYVIA
HHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLIAIDLDGTLLSKNKMISKENAEAIRKCQEEGHVVAICTGRSIVDIERLLQEVNLQCP
CEEEEEECCCEEECCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCE
IIAENGAVIYKDKTMLKRYAIQNEHALDIVEYLEEQGMYYQLYTDKGVYVPKYGEENIRK
EEECCCEEEEECHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCEECCCCCHHHHHH
EMEVIRNLDINISEEEVENIAALYLEHTAFHSIESCKSIINKELYVHKFLPFSYDQQKLK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHH
RLKEKFQHNSELIISASYWHNLEINHCDAQKGNGLYTLAEYFNIPFENTVAIGDGLNDVS
HHHHHHCCCCEEEEEEECEECEEEEECCCCCCCCEEEEHHHHCCCCCCEEEECCCCCHHH
MMQKANVSIAMGNAVNELKAMCHYETLTNEEHGVAHALYHYVIA
HHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]