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The map label for this gene is kinE [H]

Identifier: 152975646

GI number: 152975646

Start: 1993897

End: 1995735

Strand: Reverse

Name: kinE [H]

Synonym: Bcer98_1880

Alternate gene names: 152975646

Gene position: 1995735-1993897 (Counterclockwise)

Preceding gene: 152975647

Following gene: 152975645

Centisome position: 48.83

GC content: 32.79

Gene sequence:

>1839_bases
ATGAAGAAGCATGCGTTAAAAATTGGTATTAAATTTACATATAGAGGTGAAAAAGGAATGAGTGCTCGGCTAATGGAACT
CATTGATGTGAACACAATAAAAATAATGGCGGAACAGTTTTATAAATTAACAAATATGGCACACCAACTTCTTGATGCTG
AAAGAGAATGTATTTTTTCATTCGGTATAGATCAAGAAATAGATTATAGATTGACTACGATTGAAATTCCCATTTTTTTA
GACGAGCAATATTTAGGGGCTTTTATTGCTTGTCCTATAAAAAAGGATATGGTTTATTGTCAAAAATATTTCGAAACACT
TTCCAGTCTTATTGTAAATCATGCGAGAACTCAACTTCAAAACCAAAATCAAAATTTACAATTGCTTGCAAAGGAGAAAG
AACAATTATATACCATTTTACAAAATATGCCTGTTATGGTTGATGCTCTTGACCTTGATGGTGAATTTATTTTATGGAAT
CGTGAATGCAAGAGTGTAACGGGTTACACAGCAGAAGAAATGATCGGGAATCCAAAAGCTCTTGAATTATTATATCCAGA
TGCAAACTATCGTCGACAAATCCAAGAAAAGTTTGCCGTTTACGGAAGAGATTTTCGAGATTGGGAAATGAACTTAACTT
GTAAAAATGGCGAGCAGAAAACCATTATGTGGTCTAATATATCCAATCGCTTTCCGGTTTCTGGATTTAGTTACTGGGCT
GTTGGGGTCGATATTACCCATTTAAAAAAGATCGAAGCCCAGTTAAAAGAACAGACATCCGAATTGGAGCTCATTTTTCA
AGCGCTACCAGATTTATGCTTTTTAACAGAAGCTGATGGAACGATTATAGATTATAAAGCCGGTTCACCTACGAAATTTT
ATGTACCAGCAGAAGATTTTATGGGGAAAAAGTTTTATGAAGATTTGCCATCTCCTGTGGCACAACAGTTCCAAGAAGCT
ATCGATCAGGTGCAGAAAACAGAATCGATGGTTATTGTAGAGTATTCCCTTTCTTTGAAAGGAAGCATAAGCTTTTTTGA
AGCTAGATGTTTTCTACTATTGAAAGATAGAATTATGATTATTGTTCGTGATATTACTGAAAGAAAAAAAACAGAAGAGT
TATTAAATAAATCAGATACGCTTGCAGCAATTGGACAATTAGCTGCAGGAGTAGCACATGAGGTTCGAAATCCATTAACG
GTAATTAAGGGGTTTATTCAGCTCTTTCAAATAAATAAAGAAGATCAAGGGAAATACTTTGACCTTATGCTTTCAGAGAT
TGAACGAATTGAGTCTATTCTTCACGAATTTTTATCAATCGCTAAAACAGATATAATTCCTACAGAAAAGAAAAATCTTT
ATTCTATCATGAAAAATGTTGTTTCACTAATTAATACAAAAGCAATTATGACAAATATTCAAATTGAAATTATATCGGAA
TCAAAAGAATTAGTAATTGAGTGCTGTGAAAATCAATTAAAACAAGTATTTATTAACATTTTACAGAATTCAATCGAGGC
GATGCCAGATGGTGGTAAAATCTTTATTCATATGAAAACGAATAGAATAAATGAAGTGATAATTGATTTAGTAGACGAGG
GAATTGGTATACCGGAAGAACGTATTAAAAGGTTAGGAGAACCATTCTATAGTACAAAAGAAAAAGGCACTGGAATTGGT
TTAATGTTAAGCTATAAAATTATTGAACAACATCAAGGAAGAATTAGTATTATGAGTAAAGTTGGCGTTGGAACATCTGT
TACGATTTATTTACCAATGTTCCAACAAGAGAAATCTCTTTCTGACAATGAACGTCCCGTTACTATAGCGAATTCATAA

Upstream 100 bases:

>100_bases
CATTCACTTCATTAGTAGTTTGGATATTCTTATTAAAAAATAAAAATTTATTTGAATAATTACACTTCTGCTCTATACCT
GAACGGCTATTATCCGTATA

Downstream 100 bases:

>100_bases
TTAACAAATGCAACAATATCAAGTTATTTTTAAAAATATATAATCACTCTAACCGTTTATGATTTTTAAGAATGGATTAG
GGTGATTTTTTATTTGCTTG

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 612; Mature: 612

Protein sequence:

>612_residues
MKKHALKIGIKFTYRGEKGMSARLMELIDVNTIKIMAEQFYKLTNMAHQLLDAERECIFSFGIDQEIDYRLTTIEIPIFL
DEQYLGAFIACPIKKDMVYCQKYFETLSSLIVNHARTQLQNQNQNLQLLAKEKEQLYTILQNMPVMVDALDLDGEFILWN
RECKSVTGYTAEEMIGNPKALELLYPDANYRRQIQEKFAVYGRDFRDWEMNLTCKNGEQKTIMWSNISNRFPVSGFSYWA
VGVDITHLKKIEAQLKEQTSELELIFQALPDLCFLTEADGTIIDYKAGSPTKFYVPAEDFMGKKFYEDLPSPVAQQFQEA
IDQVQKTESMVIVEYSLSLKGSISFFEARCFLLLKDRIMIIVRDITERKKTEELLNKSDTLAAIGQLAAGVAHEVRNPLT
VIKGFIQLFQINKEDQGKYFDLMLSEIERIESILHEFLSIAKTDIIPTEKKNLYSIMKNVVSLINTKAIMTNIQIEIISE
SKELVIECCENQLKQVFINILQNSIEAMPDGGKIFIHMKTNRINEVIIDLVDEGIGIPEERIKRLGEPFYSTKEKGTGIG
LMLSYKIIEQHQGRISIMSKVGVGTSVTIYLPMFQQEKSLSDNERPVTIANS

Sequences:

>Translated_612_residues
MKKHALKIGIKFTYRGEKGMSARLMELIDVNTIKIMAEQFYKLTNMAHQLLDAERECIFSFGIDQEIDYRLTTIEIPIFL
DEQYLGAFIACPIKKDMVYCQKYFETLSSLIVNHARTQLQNQNQNLQLLAKEKEQLYTILQNMPVMVDALDLDGEFILWN
RECKSVTGYTAEEMIGNPKALELLYPDANYRRQIQEKFAVYGRDFRDWEMNLTCKNGEQKTIMWSNISNRFPVSGFSYWA
VGVDITHLKKIEAQLKEQTSELELIFQALPDLCFLTEADGTIIDYKAGSPTKFYVPAEDFMGKKFYEDLPSPVAQQFQEA
IDQVQKTESMVIVEYSLSLKGSISFFEARCFLLLKDRIMIIVRDITERKKTEELLNKSDTLAAIGQLAAGVAHEVRNPLT
VIKGFIQLFQINKEDQGKYFDLMLSEIERIESILHEFLSIAKTDIIPTEKKNLYSIMKNVVSLINTKAIMTNIQIEIISE
SKELVIECCENQLKQVFINILQNSIEAMPDGGKIFIHMKTNRINEVIIDLVDEGIGIPEERIKRLGEPFYSTKEKGTGIG
LMLSYKIIEQHQGRISIMSKVGVGTSVTIYLPMFQQEKSLSDNERPVTIANS
>Mature_612_residues
MKKHALKIGIKFTYRGEKGMSARLMELIDVNTIKIMAEQFYKLTNMAHQLLDAERECIFSFGIDQEIDYRLTTIEIPIFL
DEQYLGAFIACPIKKDMVYCQKYFETLSSLIVNHARTQLQNQNQNLQLLAKEKEQLYTILQNMPVMVDALDLDGEFILWN
RECKSVTGYTAEEMIGNPKALELLYPDANYRRQIQEKFAVYGRDFRDWEMNLTCKNGEQKTIMWSNISNRFPVSGFSYWA
VGVDITHLKKIEAQLKEQTSELELIFQALPDLCFLTEADGTIIDYKAGSPTKFYVPAEDFMGKKFYEDLPSPVAQQFQEA
IDQVQKTESMVIVEYSLSLKGSISFFEARCFLLLKDRIMIIVRDITERKKTEELLNKSDTLAAIGQLAAGVAHEVRNPLT
VIKGFIQLFQINKEDQGKYFDLMLSEIERIESILHEFLSIAKTDIIPTEKKNLYSIMKNVVSLINTKAIMTNIQIEIISE
SKELVIECCENQLKQVFINILQNSIEAMPDGGKIFIHMKTNRINEVIIDLVDEGIGIPEERIKRLGEPFYSTKEKGTGIG
LMLSYKIIEQHQGRISIMSKVGVGTSVTIYLPMFQQEKSLSDNERPVTIANS

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=372, Percent_Identity=29.5698924731183, Blast_Score=152, Evalue=9e-38,
Organism=Escherichia coli, GI1790436, Length=277, Percent_Identity=28.158844765343, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1786600, Length=350, Percent_Identity=22.8571428571429, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790300, Length=242, Percent_Identity=28.099173553719, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788713, Length=396, Percent_Identity=23.4848484848485, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI145693157, Length=261, Percent_Identity=26.8199233716475, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI48994928, Length=418, Percent_Identity=21.5311004784689, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788393, Length=217, Percent_Identity=23.963133640553, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786783, Length=228, Percent_Identity=24.1228070175439, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 70197; Mature: 70197

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHALKIGIKFTYRGEKGMSARLMELIDVNTIKIMAEQFYKLTNMAHQLLDAERECIFS
CCCCEEEEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
FGIDQEIDYRLTTIEIPIFLDEQYLGAFIACPIKKDMVYCQKYFETLSSLIVNHARTQLQ
CCCCCCCCEEEEEEEEEEEECCHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQNQNLQLLAKEKEQLYTILQNMPVMVDALDLDGEFILWNRECKSVTGYTAEEMIGNPKA
CCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHCCCCCE
LELLYPDANYRRQIQEKFAVYGRDFRDWEMNLTCKNGEQKTIMWSNISNRFPVSGFSYWA
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCEEEE
VGVDITHLKKIEAQLKEQTSELELIFQALPDLCFLTEADGTIIDYKAGSPTKFYVPAEDF
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCEEEEECCHHH
MGKKFYEDLPSPVAQQFQEAIDQVQKTESMVIVEYSLSLKGSISFFEARCFLLLKDRIMI
HCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCHHE
IVRDITERKKTEELLNKSDTLAAIGQLAAGVAHEVRNPLTVIKGFIQLFQINKEDQGKYF
EEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHH
DLMLSEIERIESILHEFLSIAKTDIIPTEKKNLYSIMKNVVSLINTKAIMTNIQIEIISE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECC
SKELVIECCENQLKQVFINILQNSIEAMPDGGKIFIHMKTNRINEVIIDLVDEGIGIPEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHH
RIKRLGEPFYSTKEKGTGIGLMLSYKIIEQHQGRISIMSKVGVGTSVTIYLPMFQQEKSL
HHHHCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCEEEEECCCCCCEEEEEEECHHCCCCC
SDNERPVTIANS
CCCCCCEEEECC
>Mature Secondary Structure
MKKHALKIGIKFTYRGEKGMSARLMELIDVNTIKIMAEQFYKLTNMAHQLLDAERECIFS
CCCCEEEEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
FGIDQEIDYRLTTIEIPIFLDEQYLGAFIACPIKKDMVYCQKYFETLSSLIVNHARTQLQ
CCCCCCCCEEEEEEEEEEEECCHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQNQNLQLLAKEKEQLYTILQNMPVMVDALDLDGEFILWNRECKSVTGYTAEEMIGNPKA
CCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHCCCCCE
LELLYPDANYRRQIQEKFAVYGRDFRDWEMNLTCKNGEQKTIMWSNISNRFPVSGFSYWA
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCEEEE
VGVDITHLKKIEAQLKEQTSELELIFQALPDLCFLTEADGTIIDYKAGSPTKFYVPAEDF
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCEEEEECCHHH
MGKKFYEDLPSPVAQQFQEAIDQVQKTESMVIVEYSLSLKGSISFFEARCFLLLKDRIMI
HCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCHHE
IVRDITERKKTEELLNKSDTLAAIGQLAAGVAHEVRNPLTVIKGFIQLFQINKEDQGKYF
EEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHH
DLMLSEIERIESILHEFLSIAKTDIIPTEKKNLYSIMKNVVSLINTKAIMTNIQIEIISE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECC
SKELVIECCENQLKQVFINILQNSIEAMPDGGKIFIHMKTNRINEVIIDLVDEGIGIPEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHH
RIKRLGEPFYSTKEKGTGIGLMLSYKIIEQHQGRISIMSKVGVGTSVTIYLPMFQQEKSL
HHHHCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCEEEEECCCCCCEEEEEEECHHCCCCC
SDNERPVTIANS
CCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]