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The map label for this gene is lutR [H]

Identifier: 152974827

GI number: 152974827

Start: 1140841

End: 1141488

Strand: Direct

Name: lutR [H]

Synonym: Bcer98_1023

Alternate gene names: 152974827

Gene position: 1140841-1141488 (Clockwise)

Preceding gene: 152974826

Following gene: 152974828

Centisome position: 27.91

GC content: 38.43

Gene sequence:

>648_bases
ATGATTAAAAATGGTACATTAAAACCTGGAGACAAACTTCTTCCTGTACATCAATTAGCGGAGCAGTTTCAAGTGGGCAG
ATCTGCGGTTCGTGAAGCGTTAAGCGCGCTTAGAGCGATGGGCCTAATTGAAATGAAACAAGGAGAAGGAACATATGTGA
AGAACTTTGATTCTTCGTCATTGACAAAGTCGTTAAATCATACGTTACTAATGAAGAAGGAAGACATTTTGAATTTATTA
GAAGTGCGAAAAGTGCTTGAAGTGGGGGCAGTTCGAGCAGCAGCAGCAAAGCGCACGGAAGATAACTTGCAAAATATGAG
GCATTGGTTAGATGAGATGGAAAAAGGTATTGGAGATGAAAAAGCTGGCGAAAAAGCAGATTTTCATTTTCATATGGCAA
TTGCAGAAGCTTCACATAATAAGATTTTACTTGAGCTTATGAATCATGTTTCAGAAATGATTGCTGAAACGATTGGTGAG
TCAAGGCGCATTATTTTATATGGTGAACAAACGACAGCAGAACGACTTTTAGAAGAGCATCAAGCAATTTATGAGGCAGT
ATTGGGGCAGGATATAGACTCTGCACAACAAGCAATGCTAAATCATTTAACGAATGTAGAGCATATTGTCACAGGTAAAA
AGATATAA

Upstream 100 bases:

>100_bases
TTTTAATTGTTGTTTGGATGATACTGAGGTGATTTAACTGGAATACAAATCTATTAAACCAAAAAAAATTTACGAAGAGG
TATCTGAAGCCATTTTAACA

Downstream 100 bases:

>100_bases
ATTCGTGATTTGGTCATTCAAACATTTACTGTTAGAGAAAGATTAACTGAGCGTGTTTTTAGAAAAGGAGATTTGGGCGT
TGGTTGAGCCTACTTCTTCT

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIKNGTLKPGDKLLPVHQLAEQFQVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSSLTKSLNHTLLMKKEDILNLL
EVRKVLEVGAVRAAAAKRTEDNLQNMRHWLDEMEKGIGDEKAGEKADFHFHMAIAEASHNKILLELMNHVSEMIAETIGE
SRRIILYGEQTTAERLLEEHQAIYEAVLGQDIDSAQQAMLNHLTNVEHIVTGKKI

Sequences:

>Translated_215_residues
MIKNGTLKPGDKLLPVHQLAEQFQVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSSLTKSLNHTLLMKKEDILNLL
EVRKVLEVGAVRAAAAKRTEDNLQNMRHWLDEMEKGIGDEKAGEKADFHFHMAIAEASHNKILLELMNHVSEMIAETIGE
SRRIILYGEQTTAERLLEEHQAIYEAVLGQDIDSAQQAMLNHLTNVEHIVTGKKI
>Mature_215_residues
MIKNGTLKPGDKLLPVHQLAEQFQVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSSLTKSLNHTLLMKKEDILNLL
EVRKVLEVGAVRAAAAKRTEDNLQNMRHWLDEMEKGIGDEKAGEKADFHFHMAIAEASHNKILLELMNHVSEMIAETIGE
SRRIILYGEQTTAERLLEEHQAIYEAVLGQDIDSAQQAMLNHLTNVEHIVTGKKI

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=212, Percent_Identity=32.5471698113208, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1790032, Length=211, Percent_Identity=30.3317535545024, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790780, Length=210, Percent_Identity=30.4761904761905, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1789353, Length=212, Percent_Identity=28.7735849056604, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI48994955, Length=208, Percent_Identity=28.8461538461538, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1789621, Length=217, Percent_Identity=25.8064516129032, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24090; Mature: 24090

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNGTLKPGDKLLPVHQLAEQFQVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCHHH
LTKSLNHTLLMKKEDILNLLEVRKVLEVGAVRAAAAKRTEDNLQNMRHWLDEMEKGIGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KAGEKADFHFHMAIAEASHNKILLELMNHVSEMIAETIGESRRIILYGEQTTAERLLEEH
CCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
QAIYEAVLGQDIDSAQQAMLNHLTNVEHIVTGKKI
HHHHHHHHCCCCCHHHHHHHHHHCCHHHHHCCCCC
>Mature Secondary Structure
MIKNGTLKPGDKLLPVHQLAEQFQVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCHHH
LTKSLNHTLLMKKEDILNLLEVRKVLEVGAVRAAAAKRTEDNLQNMRHWLDEMEKGIGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KAGEKADFHFHMAIAEASHNKILLELMNHVSEMIAETIGESRRIILYGEQTTAERLLEEH
CCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
QAIYEAVLGQDIDSAQQAMLNHLTNVEHIVTGKKI
HHHHHHHHCCCCCHHHHHHHHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]