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The map label for this gene is dacB [H]

Identifier: 152974802

GI number: 152974802

Start: 1113513

End: 1114784

Strand: Direct

Name: dacB [H]

Synonym: Bcer98_0998

Alternate gene names: 152974802

Gene position: 1113513-1114784 (Clockwise)

Preceding gene: 152974798

Following gene: 152974803

Centisome position: 27.25

GC content: 38.44

Gene sequence:

>1272_bases
ATGGATAAATGTAAGAAAATTATGTTTTTTGTTTTGGTGATATGTTTGTTCTTTGTTACCCCGTTACCATTACATGCAGA
GACGGGAATGGAGCCAGCACCAGCACCTGGACAAGGTCCAAATGTGTTTGGAAAGTTTGCTGTTTCGATTGATGCGAAGA
CGGGGGATGTGCTATATGATAAGAATGCACATGAACATGCGTTTCCGGCTAGTATGACAAAGGTATTAACGGCTATTTTA
CTTATGGAGCATACGAAGCCTGAAGAGCAATTTACACTTTCACAAGCGGCATTAGACCAGGAGAAGAGTAATTATCAACT
GGAGTTTCAAGCTGGAGAGACGGTAGATCGGAATACGGCGCTAATGATTTTAATGGTGCTGAGTGCGAATGATGTCGCGT
ATGCGATAGGGGAGCATATTGGAGGAAGTATAGAAAACTTTGCGCATATGATGAATGAGAAGGCAAAGCAGCTTGGCGCG
ACGGATAGTCATTTTGTTACACCGAATGGTTTGCACGATCCAAATCATTATACGACCGCATACGACATGGCAATGATTAC
GAGAGGTGTACAAAAGTATCCTGAAATTTTACAGGCGATGAATACAAAGCGTACGACGGTTACAACTTCTAGACAAACGG
TTTCTATTTTTAATAAAGCAAATTATTTTGAGAATCCGTATAGTATTGGTGGAAAGACAGGTTTTACAAATGAAGCACGT
AACACGCTTGTTTTATTAAATGAGAAAGATGGAAATCGAATTATTAATGTTGTGATGGCTTCGCAAAAGCCGGAAATCTA
TGAAGATATGAAGCAGATGGCTGAATATGCATTTCCGCAGTTTACGAAACAGCTCGTACTCGATAAGAATTATTGGCATG
AAAGAGCAACGTATTTAGATAGAGATATAGATTGTGAATTAGAGAAAAGTGCAGAACTGATGCTGAAAAAAGGGGAAGGA
AAAAATGTAAAAACAGTTTTTCGAAAAGCTCCTGTAGATACAGATTCTTTATATGAAAGAGGAATTCATCGCGGTGAGGT
AGTGGGAACTGTAGATATAACAAAGAATAATGAAACGATTGCAAGCATTCATGTTCTTTCAAAAGAAGATGTGCAGTTTG
CGGCAGCGAAGGAGAAGATAGAAAAAACGGGACAAACGTATTCTTTTGAAGGGATTGCAGCTTGTGTTGGAATTGGTATG
ATTATACTTTTATTACTTGGTATATATATTACGAGAAAATCGAAAAAGAATGCGGTTTCAGAAGAAAAATAG

Upstream 100 bases:

>100_bases
TGTGATATAGTGAAGAATGATGGTTGTTTTCCAGTTTGTTTCCAATTCAAAGGTGGGTTAGTGATTTTTATATATAAGTT
ACGAAAAAAGGGAGATTTAT

Downstream 100 bases:

>100_bases
GCAATGATGAATTGCCTATTTTTTATTGCGTTTTCGAGTTGTTCATTATAGTATTAAATTGAGTTCTTTTTAAAGAACAA
GATAAAGTATTCTATTTTTT

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MDKCKKIMFFVLVICLFFVTPLPLHAETGMEPAPAPGQGPNVFGKFAVSIDAKTGDVLYDKNAHEHAFPASMTKVLTAIL
LMEHTKPEEQFTLSQAALDQEKSNYQLEFQAGETVDRNTALMILMVLSANDVAYAIGEHIGGSIENFAHMMNEKAKQLGA
TDSHFVTPNGLHDPNHYTTAYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKANYFENPYSIGGKTGFTNEAR
NTLVLLNEKDGNRIINVVMASQKPEIYEDMKQMAEYAFPQFTKQLVLDKNYWHERATYLDRDIDCELEKSAELMLKKGEG
KNVKTVFRKAPVDTDSLYERGIHRGEVVGTVDITKNNETIASIHVLSKEDVQFAAAKEKIEKTGQTYSFEGIAACVGIGM
IILLLLGIYITRKSKKNAVSEEK

Sequences:

>Translated_423_residues
MDKCKKIMFFVLVICLFFVTPLPLHAETGMEPAPAPGQGPNVFGKFAVSIDAKTGDVLYDKNAHEHAFPASMTKVLTAIL
LMEHTKPEEQFTLSQAALDQEKSNYQLEFQAGETVDRNTALMILMVLSANDVAYAIGEHIGGSIENFAHMMNEKAKQLGA
TDSHFVTPNGLHDPNHYTTAYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKANYFENPYSIGGKTGFTNEAR
NTLVLLNEKDGNRIINVVMASQKPEIYEDMKQMAEYAFPQFTKQLVLDKNYWHERATYLDRDIDCELEKSAELMLKKGEG
KNVKTVFRKAPVDTDSLYERGIHRGEVVGTVDITKNNETIASIHVLSKEDVQFAAAKEKIEKTGQTYSFEGIAACVGIGM
IILLLLGIYITRKSKKNAVSEEK
>Mature_423_residues
MDKCKKIMFFVLVICLFFVTPLPLHAETGMEPAPAPGQGPNVFGKFAVSIDAKTGDVLYDKNAHEHAFPASMTKVLTAIL
LMEHTKPEEQFTLSQAALDQEKSNYQLEFQAGETVDRNTALMILMVLSANDVAYAIGEHIGGSIENFAHMMNEKAKQLGA
TDSHFVTPNGLHDPNHYTTAYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKANYFENPYSIGGKTGFTNEAR
NTLVLLNEKDGNRIINVVMASQKPEIYEDMKQMAEYAFPQFTKQLVLDKNYWHERATYLDRDIDCELEKSAELMLKKGEG
KNVKTVFRKAPVDTDSLYERGIHRGEVVGTVDITKNNETIASIHVLSKEDVQFAAAKEKIEKTGQTYSFEGIAACVGIGM
IILLLLGIYITRKSKKNAVSEEK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=377, Percent_Identity=24.4031830238727, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI87082022, Length=364, Percent_Identity=25.5494505494505, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786851, Length=379, Percent_Identity=23.4828496042216, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87082054, Length=225, Percent_Identity=27.5555555555556, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47261; Mature: 47261

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKCKKIMFFVLVICLFFVTPLPLHAETGMEPAPAPGQGPNVFGKFAVSIDAKTGDVLYD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEC
KNAHEHAFPASMTKVLTAILLMEHTKPEEQFTLSQAALDQEKSNYQLEFQAGETVDRNTA
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCE
LMILMVLSANDVAYAIGEHIGGSIENFAHMMNEKAKQLGATDSHFVTPNGLHDPNHYTTA
EEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHH
YDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKANYFENPYSIGGKTGFTNEAR
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
NTLVLLNEKDGNRIINVVMASQKPEIYEDMKQMAEYAFPQFTKQLVLDKNYWHERATYLD
CEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
RDIDCELEKSAELMLKKGEGKNVKTVFRKAPVDTDSLYERGIHRGEVVGTVDITKNNETI
CCCCCCHHCCHHHHEECCCCCCHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEEECCCCEE
ASIHVLSKEDVQFAAAKEKIEKTGQTYSFEGIAACVGIGMIILLLLGIYITRKSKKNAVS
EEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
EEK
CCC
>Mature Secondary Structure
MDKCKKIMFFVLVICLFFVTPLPLHAETGMEPAPAPGQGPNVFGKFAVSIDAKTGDVLYD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEC
KNAHEHAFPASMTKVLTAILLMEHTKPEEQFTLSQAALDQEKSNYQLEFQAGETVDRNTA
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCE
LMILMVLSANDVAYAIGEHIGGSIENFAHMMNEKAKQLGATDSHFVTPNGLHDPNHYTTA
EEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHH
YDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKANYFENPYSIGGKTGFTNEAR
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
NTLVLLNEKDGNRIINVVMASQKPEIYEDMKQMAEYAFPQFTKQLVLDKNYWHERATYLD
CEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
RDIDCELEKSAELMLKKGEGKNVKTVFRKAPVDTDSLYERGIHRGEVVGTVDITKNNETI
CCCCCCHHCCHHHHEECCCCCCHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEEECCCCEE
ASIHVLSKEDVQFAAAKEKIEKTGQTYSFEGIAACVGIGMIILLLLGIYITRKSKKNAVS
EEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
EEK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]