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The map label for this gene is hpaH [H]

Identifier: 152974775

GI number: 152974775

Start: 1087605

End: 1089077

Strand: Direct

Name: hpaH [H]

Synonym: Bcer98_0966

Alternate gene names: 152974775

Gene position: 1087605-1089077 (Clockwise)

Preceding gene: 152974774

Following gene: 152974776

Centisome position: 26.61

GC content: 40.46

Gene sequence:

>1473_bases
ATGCCAGCAAAAACAGGAAAAGAATATATCGAAAGAGTAGATCAAGCAAATGCCAATGTATGGATTGACGGGAAGAGAGT
AGAAGGAAACATCTCAGAACATCCTGCTTTTAAAGGAGTAATGAAAACGCAAGCTGAGTTATATGATATGCAGCACGATC
CTGAGAAAGCAGAGTATATGACATATACTTCTCCAACTTCAGGAGAGCGGGTCGGCACTTCCTTTTTGCAACCAAAAACA
AAAGAAGACTTAGCAAAGCGCCGCAAAATGATTCAAGAGTGGGCAAAGTACAACGGCGGTATGATGGGGCGCTCTCCAGA
TTATATTAACGCGGGAATGATGGCTTACGGATCGGCTGCTGAAATGTTTGGAAAACAAGATGCGTTTTATGCGAAAAATA
TGGCGGAGTATTATGAGTATTGTCGCGAGAATGATTTATCTTTAACACATACATTAATTCAGCCGCAAGTGAATCGTGCG
GTTAATGCATCGCAATTATCTGATCCTTATATTGCTGCGCGCATTGTAGAAAAAACGTCAGAAGGCGTAGTCATTCGCGG
AGCGCGTTTACTCGCAACGCAAGGAGGAATTACCGATGAAATTATGGTGTTTCCTTCGACTCTTTTAAAACAATCCGAAG
AGGAAAATCCATATGCATATGCTTTTTCAATTCCAAATCATACACCGGGCCTGAAGTTTATTTGCCGTGAATCATTTGAT
TACGGAAAGTCTCATTTTGATCATCCGCTTGGATCGAGATTTGAAGAAATGGATACGATTGTCGTATTTGATGATGTCGT
AGTGCCTTGGAATCGGGTGTTTGCCTTAGGGGATGTTGGCGTTTGTAATCAAGCTTACAATGAAAGTAATGCGGTTGTTC
ATATGACGCATCAAGTTGTCTCAAAAAATATCGCAAAAACGGAATTTGTATTAGGAATTTTACAGCTCATGGTAGAAACG
ATTAATATCGGTCAGTTTCAACATGTACAAGAAAAGATGTCGGAAGTTATTATGGCGCTTGAAACAGTGAAAGCATACGT
AACGGCGGCAGAAGCGAATGCGAAGCTGGATAAGTGGGGCATTATGACACCGGACTTTACACCATTAAATGTTGCACGCC
ATTACTATCCAAAAATTTACCCAAGATTTAGTGAAATTATGCAACTGCTTGGAGCAAGTGGTTTAATGGCAATTCCATCC
GAATCAGATTTTCAATCGGAATTACGCCCTGATTTAGATAAATATTTACAAGCGGCAAATGGGGACGCATATAATCGTGT
GAAATTATATCGACTAGCTTGGGATGTTTGTATGAGTGCATTTGGCACGAGACAGACATTGTATGAACGCTTCTTTTTCG
GAGATCCAGTTAGAATGGCAAGCGCGCTATATAATGGCTACGATAAGCAAGTATATGTGGACCGCGTAAAAGAGTTTTTA
AATCGCTCGGAAGAGTTAATCGAAAAAGTATAA

Upstream 100 bases:

>100_bases
TTGCAATCGGAGATCATCATATTCCGAAGTTTGGCACAAAGAAATAACAAGCTGCAGATGAAAGTATAAAAACAATAGTT
TCATGAAAGGGGAGAGTGAA

Downstream 100 bases:

>100_bases
AAATGTGGAGGTGCAACAAATGGAGTTTAATATTATTCGCATTGCAAGAACAATTCTAAATGTGAAAGATTTGGATCGTT
CGCGAGAATTTTATGTAAAC

Product: 4-hydroxyphenylacetate 3-monooxygenase, oxygenase subunit

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-hydroxyphenylacetate-3-hydroxylase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MPAKTGKEYIERVDQANANVWIDGKRVEGNISEHPAFKGVMKTQAELYDMQHDPEKAEYMTYTSPTSGERVGTSFLQPKT
KEDLAKRRKMIQEWAKYNGGMMGRSPDYINAGMMAYGSAAEMFGKQDAFYAKNMAEYYEYCRENDLSLTHTLIQPQVNRA
VNASQLSDPYIAARIVEKTSEGVVIRGARLLATQGGITDEIMVFPSTLLKQSEEENPYAYAFSIPNHTPGLKFICRESFD
YGKSHFDHPLGSRFEEMDTIVVFDDVVVPWNRVFALGDVGVCNQAYNESNAVVHMTHQVVSKNIAKTEFVLGILQLMVET
INIGQFQHVQEKMSEVIMALETVKAYVTAAEANAKLDKWGIMTPDFTPLNVARHYYPKIYPRFSEIMQLLGASGLMAIPS
ESDFQSELRPDLDKYLQAANGDAYNRVKLYRLAWDVCMSAFGTRQTLYERFFFGDPVRMASALYNGYDKQVYVDRVKEFL
NRSEELIEKV

Sequences:

>Translated_490_residues
MPAKTGKEYIERVDQANANVWIDGKRVEGNISEHPAFKGVMKTQAELYDMQHDPEKAEYMTYTSPTSGERVGTSFLQPKT
KEDLAKRRKMIQEWAKYNGGMMGRSPDYINAGMMAYGSAAEMFGKQDAFYAKNMAEYYEYCRENDLSLTHTLIQPQVNRA
VNASQLSDPYIAARIVEKTSEGVVIRGARLLATQGGITDEIMVFPSTLLKQSEEENPYAYAFSIPNHTPGLKFICRESFD
YGKSHFDHPLGSRFEEMDTIVVFDDVVVPWNRVFALGDVGVCNQAYNESNAVVHMTHQVVSKNIAKTEFVLGILQLMVET
INIGQFQHVQEKMSEVIMALETVKAYVTAAEANAKLDKWGIMTPDFTPLNVARHYYPKIYPRFSEIMQLLGASGLMAIPS
ESDFQSELRPDLDKYLQAANGDAYNRVKLYRLAWDVCMSAFGTRQTLYERFFFGDPVRMASALYNGYDKQVYVDRVKEFL
NRSEELIEKV
>Mature_489_residues
PAKTGKEYIERVDQANANVWIDGKRVEGNISEHPAFKGVMKTQAELYDMQHDPEKAEYMTYTSPTSGERVGTSFLQPKTK
EDLAKRRKMIQEWAKYNGGMMGRSPDYINAGMMAYGSAAEMFGKQDAFYAKNMAEYYEYCRENDLSLTHTLIQPQVNRAV
NASQLSDPYIAARIVEKTSEGVVIRGARLLATQGGITDEIMVFPSTLLKQSEEENPYAYAFSIPNHTPGLKFICRESFDY
GKSHFDHPLGSRFEEMDTIVVFDDVVVPWNRVFALGDVGVCNQAYNESNAVVHMTHQVVSKNIAKTEFVLGILQLMVETI
NIGQFQHVQEKMSEVIMALETVKAYVTAAEANAKLDKWGIMTPDFTPLNVARHYYPKIYPRFSEIMQLLGASGLMAIPSE
SDFQSELRPDLDKYLQAANGDAYNRVKLYRLAWDVCMSAFGTRQTLYERFFFGDPVRMASALYNGYDKQVYVDRVKEFLN
RSEELIEKV

Specific function: Utilizes FADH(2) supplied by hpaI, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 55661; Mature: 55530

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKTGKEYIERVDQANANVWIDGKRVEGNISEHPAFKGVMKTQAELYDMQHDPEKAEYM
CCCCCHHHHHHHHHHCCCCEEEECCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCEE
TYTSPTSGERVGTSFLQPKTKEDLAKRRKMIQEWAKYNGGMMGRSPDYINAGMMAYGSAA
EECCCCCCCHHCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHH
EMFGKQDAFYAKNMAEYYEYCRENDLSLTHTLIQPQVNRAVNASQLSDPYIAARIVEKTS
HHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCC
EGVVIRGARLLATQGGITDEIMVFPSTLLKQSEEENPYAYAFSIPNHTPGLKFICRESFD
CCEEEECCEEEEECCCCCCHHHCCCHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
YGKSHFDHPLGSRFEEMDTIVVFDDVVVPWNRVFALGDVGVCNQAYNESNAVVHMTHQVV
CCHHHCCCHHHHHHHHCCEEEEECCEECCCHHEEEECCCHHHHHHCCCCCCEEEEHHHHH
SKNIAKTEFVLGILQLMVETINIGQFQHVQEKMSEVIMALETVKAYVTAAEANAKLDKWG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
IMTPDFTPLNVARHYYPKIYPRFSEIMQLLGASGLMAIPSESDFQSELRPDLDKYLQAAN
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCHHHHHHHCC
GDAYNRVKLYRLAWDVCMSAFGTRQTLYERFFFGDPVRMASALYNGYDKQVYVDRVKEFL
CCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHH
NRSEELIEKV
HHHHHHHHCC
>Mature Secondary Structure 
PAKTGKEYIERVDQANANVWIDGKRVEGNISEHPAFKGVMKTQAELYDMQHDPEKAEYM
CCCCHHHHHHHHHHCCCCEEEECCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCEE
TYTSPTSGERVGTSFLQPKTKEDLAKRRKMIQEWAKYNGGMMGRSPDYINAGMMAYGSAA
EECCCCCCCHHCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHH
EMFGKQDAFYAKNMAEYYEYCRENDLSLTHTLIQPQVNRAVNASQLSDPYIAARIVEKTS
HHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCC
EGVVIRGARLLATQGGITDEIMVFPSTLLKQSEEENPYAYAFSIPNHTPGLKFICRESFD
CCEEEECCEEEEECCCCCCHHHCCCHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
YGKSHFDHPLGSRFEEMDTIVVFDDVVVPWNRVFALGDVGVCNQAYNESNAVVHMTHQVV
CCHHHCCCHHHHHHHHCCEEEEECCEECCCHHEEEECCCHHHHHHCCCCCCEEEEHHHHH
SKNIAKTEFVLGILQLMVETINIGQFQHVQEKMSEVIMALETVKAYVTAAEANAKLDKWG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
IMTPDFTPLNVARHYYPKIYPRFSEIMQLLGASGLMAIPSESDFQSELRPDLDKYLQAAN
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCHHHHHHHCC
GDAYNRVKLYRLAWDVCMSAFGTRQTLYERFFFGDPVRMASALYNGYDKQVYVDRVKEFL
CCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHH
NRSEELIEKV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA