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The map label for this gene is glpK

Identifier: 152974623

GI number: 152974623

Start: 922678

End: 924168

Strand: Direct

Name: glpK

Synonym: Bcer98_0807

Alternate gene names: 152974623

Gene position: 922678-924168 (Clockwise)

Preceding gene: 152974622

Following gene: 152974624

Centisome position: 22.58

GC content: 39.97

Gene sequence:

>1491_bases
ATGGAAACATATATTCTTTCATTAGATCAAGGTACAACAAGTTCACGTGCCATTCTGTTTAATAAAAAAGGAGAAATTGT
TCATTCTGCTCAAAAAGAATTTACACAATATTTTCCAAAGCCAGGTTGGGTAGAACATAATGCACAAGAGATTTGGGGGT
CCATTCTAGCGGTTATCGCAACTTGCTTAAGTGAAGCGGATGTGAAGCCAGAACAAATCGCTGGTATTGGGATTACAAAC
CAACGTGAAACAGCAGTTGTTTGGGAGAAAGCAACCGGGAAACCAGTTTATAATGCAATTGTATGGCAATCTCGTCAAAC
AGCTGAAATTTGTGAAGAGTTAAAAGAAAAAGGCTATGGCGATATGGTTCGCGAAAAAACAGGTCTTTTAATTGATGCAT
ATTTCTCAGGTACGAAAGTAAAGTGGATTTTAGATAACGTTGAAGGTGCAAGAGAAAAAGCAGAGCGCGGTGAATTATTA
TTCGGTACAATTGATACATGGCTTGTATGGAAATTGTCTGGTGGTAAAGCACACGTAACAGATTATTCAAATGCATCACG
CACATTAATGTTCAATATTCATGACTTAAAATGGGATGATGAATTATTAGAAATACTAACAGTACCAAAGAGCATGTTAC
CAGAAGTACGTCCATCATCTGAAGTATACGGTCATACAGTTGATTATCATTTCTTCTCTCAAAATGTACCGATTGCTGGT
GTGGCAGGTGACCAACAAGCAGCGTTATTTGGGCAAGCGTGCTTCAGTGAAGGGATGGCGAAAAACACATACGGAACAGG
TTGCTTCATGTTAATGAATACAGGTGAAACAGCAGTTAACTCTAATCACGGTCTATTAACAACAATTGCGTGGGGGTTAA
ATGGTAAAGTAAATTATGCATTAGAAGGAAGTATTTTCGTAGCTGGTTCAGCAATCCAGTGGTTACGAGATGGCATGCGT
ATGGTTAATGATGCAAGTGAGAGTGAGGAGTATGCATCACGCGTGGAATCAACGGACGGTGTATACGTTGTACCAGCATT
TGTAGGACTTGGAACGCCGTATTGGGACAGTGAAGTACGCGGAGCTGTGTTTGGTGTAACGCGCGGTACAACGAAAGAGC
ACTTCATTCGTGCAACATTAGAATCATTAGGATATCAAACAAGAGATGTATTATGTGCAATGGAAGCAGATTCTGGCATT
GAGTTGAAAACGTTACGCGTTGATGGCGGTGCAGTGAAAAATAACTTCTTAATGCAGTTCCAAAGCGATATGTTACAGGT
TCCTGTAGAGCGTCCAATGATTAGTGAAACGACAGCTTTAGGGGCTGCATATTTAGCAGGTCTAGCTGTTGGATATTGGG
AAAGTCAAGAAGAAATTAAAGCGCAGTGGGATATGGATCGAAGCTTTACACCAGAAATGGAGAAAGAAAGAAGCGAAGAG
CTCTATTCTGGATGGAAAAAAGCAATTGAAGCAACAAAAGCTTTTAAATAA

Upstream 100 bases:

>100_bases
GTGATTGCAACGGTTATTATACTAGCACTTTCTTATATAGCAGGTAAAAAGAATAGAAACGATACAAATAGTAGAAAAGT
AGCATAAGGGGGAAGTAAAT

Downstream 100 bases:

>100_bases
TCATAAACAGTGTTATAATGATGGTAAGTTAATAATTCGGTAGGAGATATGGAGAGACCATGACACGCTATAGAGCGATT
TCTATAGTGGTGTTTGTGGT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
METYILSLDQGTTSSRAILFNKKGEIVHSAQKEFTQYFPKPGWVEHNAQEIWGSILAVIATCLSEADVKPEQIAGIGITN
QRETAVVWEKATGKPVYNAIVWQSRQTAEICEELKEKGYGDMVREKTGLLIDAYFSGTKVKWILDNVEGAREKAERGELL
FGTIDTWLVWKLSGGKAHVTDYSNASRTLMFNIHDLKWDDELLEILTVPKSMLPEVRPSSEVYGHTVDYHFFSQNVPIAG
VAGDQQAALFGQACFSEGMAKNTYGTGCFMLMNTGETAVNSNHGLLTTIAWGLNGKVNYALEGSIFVAGSAIQWLRDGMR
MVNDASESEEYASRVESTDGVYVVPAFVGLGTPYWDSEVRGAVFGVTRGTTKEHFIRATLESLGYQTRDVLCAMEADSGI
ELKTLRVDGGAVKNNFLMQFQSDMLQVPVERPMISETTALGAAYLAGLAVGYWESQEEIKAQWDMDRSFTPEMEKERSEE
LYSGWKKAIEATKAFK

Sequences:

>Translated_496_residues
METYILSLDQGTTSSRAILFNKKGEIVHSAQKEFTQYFPKPGWVEHNAQEIWGSILAVIATCLSEADVKPEQIAGIGITN
QRETAVVWEKATGKPVYNAIVWQSRQTAEICEELKEKGYGDMVREKTGLLIDAYFSGTKVKWILDNVEGAREKAERGELL
FGTIDTWLVWKLSGGKAHVTDYSNASRTLMFNIHDLKWDDELLEILTVPKSMLPEVRPSSEVYGHTVDYHFFSQNVPIAG
VAGDQQAALFGQACFSEGMAKNTYGTGCFMLMNTGETAVNSNHGLLTTIAWGLNGKVNYALEGSIFVAGSAIQWLRDGMR
MVNDASESEEYASRVESTDGVYVVPAFVGLGTPYWDSEVRGAVFGVTRGTTKEHFIRATLESLGYQTRDVLCAMEADSGI
ELKTLRVDGGAVKNNFLMQFQSDMLQVPVERPMISETTALGAAYLAGLAVGYWESQEEIKAQWDMDRSFTPEMEKERSEE
LYSGWKKAIEATKAFK
>Mature_496_residues
METYILSLDQGTTSSRAILFNKKGEIVHSAQKEFTQYFPKPGWVEHNAQEIWGSILAVIATCLSEADVKPEQIAGIGITN
QRETAVVWEKATGKPVYNAIVWQSRQTAEICEELKEKGYGDMVREKTGLLIDAYFSGTKVKWILDNVEGAREKAERGELL
FGTIDTWLVWKLSGGKAHVTDYSNASRTLMFNIHDLKWDDELLEILTVPKSMLPEVRPSSEVYGHTVDYHFFSQNVPIAG
VAGDQQAALFGQACFSEGMAKNTYGTGCFMLMNTGETAVNSNHGLLTTIAWGLNGKVNYALEGSIFVAGSAIQWLRDGMR
MVNDASESEEYASRVESTDGVYVVPAFVGLGTPYWDSEVRGAVFGVTRGTTKEHFIRATLESLGYQTRDVLCAMEADSGI
ELKTLRVDGGAVKNNFLMQFQSDMLQVPVERPMISETTALGAAYLAGLAVGYWESQEEIKAQWDMDRSFTPEMEKERSEE
LYSGWKKAIEATKAFK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI42794763, Length=500, Percent_Identity=46, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI189409120, Length=496, Percent_Identity=46.1693548387097, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI4504007, Length=496, Percent_Identity=46.1693548387097, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=493, Percent_Identity=45.6389452332657, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI88196792, Length=503, Percent_Identity=32.6043737574553, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=63.2860040567951, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=528, Percent_Identity=23.8636363636364, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1789168, Length=493, Percent_Identity=24.7464503042596, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789987, Length=488, Percent_Identity=24.1803278688525, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787789, Length=522, Percent_Identity=23.7547892720307, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17535599, Length=498, Percent_Identity=41.1646586345382, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=25.7692307692308, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI71986147, Length=272, Percent_Identity=30.5147058823529, Blast_Score=141, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6321755, Length=497, Percent_Identity=37.2233400402414, Blast_Score=313, Evalue=3e-86,
Organism=Drosophila melanogaster, GI17864214, Length=501, Percent_Identity=43.1137724550898, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24654573, Length=432, Percent_Identity=44.9074074074074, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655555, Length=498, Percent_Identity=38.7550200803213, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655560, Length=498, Percent_Identity=38.7550200803213, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655570, Length=498, Percent_Identity=38.7550200803213, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655574, Length=498, Percent_Identity=38.7550200803213, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655565, Length=498, Percent_Identity=38.7550200803213, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=522, Percent_Identity=31.0344827586207, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24652871, Length=489, Percent_Identity=31.6973415132924, Blast_Score=265, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24656367, Length=508, Percent_Identity=27.5590551181102, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI21357867, Length=523, Percent_Identity=26.5774378585086, Blast_Score=208, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24656372, Length=355, Percent_Identity=28.7323943661972, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI45552909, Length=302, Percent_Identity=26.4900662251656, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_BACCN (A7GLY7)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001374140.1
- ProteinModelPortal:   A7GLY7
- SMR:   A7GLY7
- STRING:   A7GLY7
- EnsemblBacteria:   EBBACT00000037162
- GeneID:   5346306
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_0807
- eggNOG:   COG0554
- GeneTree:   EBGT00050000001374
- HOGENOM:   HBG511469
- OMA:   QDRRTEE
- ProtClustDB:   PRK00047
- BioCyc:   BCER315749:BCER98_0807-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55072; Mature: 55072

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METYILSLDQGTTSSRAILFNKKGEIVHSAQKEFTQYFPKPGWVEHNAQEIWGSILAVIA
CCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
TCLSEADVKPEQIAGIGITNQRETAVVWEKATGKPVYNAIVWQSRQTAEICEELKEKGYG
HHHHHCCCCHHHEEECCCCCCCCEEEEEECCCCCCHHHHHEECCCCHHHHHHHHHHCCCC
DMVREKTGLLIDAYFSGTKVKWILDNVEGAREKAERGELLFGTIDTWLVWKLSGGKAHVT
HHHHHCCCCEEEEEECCCEEEEEEECCHHHHHHHHCCCEEEEEECEEEEEEECCCEEEEE
DYSNASRTLMFNIHDLKWDDELLEILTVPKSMLPEVRPSSEVYGHTVDYHFFSQNVPIAG
ECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEEEECCCCCEEE
VAGDQQAALFGQACFSEGMAKNTYGTGCFMLMNTGETAVNSNHGLLTTIAWGLNGKVNYA
ECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEECCCCCEEEEEEECCCCEEEEE
LEGSIFVAGSAIQWLRDGMRMVNDASESEEYASRVESTDGVYVVPAFVGLGTPYWDSEVR
EECCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCC
GAVFGVTRGTTKEHFIRATLESLGYQTRDVLCAMEADSGIELKTLRVDGGAVKNNFLMQF
CEEEEEECCCCHHHHHHHHHHHCCCCCHHEEEEEECCCCCEEEEEEECCCCCCCHHHHHH
QSDMLQVPVERPMISETTALGAAYLAGLAVGYWESQEEIKAQWDMDRSFTPEMEKERSEE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
LYSGWKKAIEATKAFK
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
METYILSLDQGTTSSRAILFNKKGEIVHSAQKEFTQYFPKPGWVEHNAQEIWGSILAVIA
CCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
TCLSEADVKPEQIAGIGITNQRETAVVWEKATGKPVYNAIVWQSRQTAEICEELKEKGYG
HHHHHCCCCHHHEEECCCCCCCCEEEEEECCCCCCHHHHHEECCCCHHHHHHHHHHCCCC
DMVREKTGLLIDAYFSGTKVKWILDNVEGAREKAERGELLFGTIDTWLVWKLSGGKAHVT
HHHHHCCCCEEEEEECCCEEEEEEECCHHHHHHHHCCCEEEEEECEEEEEEECCCEEEEE
DYSNASRTLMFNIHDLKWDDELLEILTVPKSMLPEVRPSSEVYGHTVDYHFFSQNVPIAG
ECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEEEECCCCCEEE
VAGDQQAALFGQACFSEGMAKNTYGTGCFMLMNTGETAVNSNHGLLTTIAWGLNGKVNYA
ECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEECCCCCEEEEEEECCCCEEEEE
LEGSIFVAGSAIQWLRDGMRMVNDASESEEYASRVESTDGVYVVPAFVGLGTPYWDSEVR
EECCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCC
GAVFGVTRGTTKEHFIRATLESLGYQTRDVLCAMEADSGIELKTLRVDGGAVKNNFLMQF
CEEEEEECCCCHHHHHHHHHHHCCCCCHHEEEEEECCCCCEEEEEEECCCCCCCHHHHHH
QSDMLQVPVERPMISETTALGAAYLAGLAVGYWESQEEIKAQWDMDRSFTPEMEKERSEE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
LYSGWKKAIEATKAFK
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA