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The map label for this gene is glpF [H]

Identifier: 152974622

GI number: 152974622

Start: 921843

End: 922664

Strand: Direct

Name: glpF [H]

Synonym: Bcer98_0806

Alternate gene names: 152974622

Gene position: 921843-922664 (Clockwise)

Preceding gene: 152974621

Following gene: 152974623

Centisome position: 22.56

GC content: 39.54

Gene sequence:

>822_bases
ATGTCAGCATTTTTAGGAGAGTTAATAGGGACAGCGTTATTAATCGTTCTTGGTGGCGGTGTTTGTGCTGGTGTAAGTTT
AAAGAAATCGTTTGCTAAGGATTCTGGCTGGATTGTAATTACAATGGGGTGGGGCTTAGCCGTTGCAGTAGCAGCATATG
CTGTTGGATCTATTAGTGGAGCACATTTGAACCCTGCCTTAACAATAGGACTAGCGTTTAAGGGAACGTTCCCATGGAGC
GATGTTCCAGGATATATCGTAGCACAAATGATTGGGGCAATAATCGGTGCTGTTATCGTATATTTACATTACTTACCACA
TTGGAAAGAAACGGAAGATCCAGGGGCAAAGTTAGGTGTGTTTGCAACAGGACCAGCAATTCCAAACACATTTGCAAACC
TTCTAAGTGAAATGATTGGTACATTCGTTTTAGTATTTGGTATATTAGCAATTGGTGCAAATAAATTTGCAGATGGCTTA
AACCCATTTATCGTAGGATTCTTAATTGTAAGTATTGGTTTATCATTAGGTGGAACAACGGGATATGCGATTAACCCAGC
GCGTGATTTGGGACCTCGTATTGCACATTTCTTTCTTCCAATTGCAGGAAAAGGCGGATCGAATTGGAAGTATGCATGGA
TTCCAGTTGTAGGCCCGATTTTAGGCGGATCACTTGCAGGTGTATTTCATCAAGTTGTATTTGAAGAAAAGCAAAATGCA
GCACTTATATATGTGGTGATTGCAACGGTTATTATACTAGCACTTTCTTATATAGCAGGTAAAAAGAATAGAAACGATAC
AAATAGTAGAAAAGTAGCATAA

Upstream 100 bases:

>100_bases
AGGCATGTAGGCTGTGGATTTTTTCTTTTGAAAAAACGAATGGCCATTCATTTTCTCTCCTTCACATTGTAAGCGTTTAA
TTTGATTGTGGAGGGATTTT

Downstream 100 bases:

>100_bases
GGGGGAAGTAAATATGGAAACATATATTCTTTCATTAGATCAAGGTACAACAAGTTCACGTGCCATTCTGTTTAATAAAA
AAGGAGAAATTGTTCATTCT

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISGAHLNPALTIGLAFKGTFPWS
DVPGYIVAQMIGAIIGAVIVYLHYLPHWKETEDPGAKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGL
NPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGVFHQVVFEEKQNA
ALIYVVIATVIILALSYIAGKKNRNDTNSRKVA

Sequences:

>Translated_273_residues
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISGAHLNPALTIGLAFKGTFPWS
DVPGYIVAQMIGAIIGAVIVYLHYLPHWKETEDPGAKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGL
NPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGVFHQVVFEEKQNA
ALIYVVIATVIILALSYIAGKKNRNDTNSRKVA
>Mature_272_residues
SAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISGAHLNPALTIGLAFKGTFPWSD
VPGYIVAQMIGAIIGAVIVYLHYLPHWKETEDPGAKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLN
PFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGVFHQVVFEEKQNAA
LIYVVIATVIILALSYIAGKKNRNDTNSRKVA

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=269, Percent_Identity=36.4312267657993, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=35.4838709677419, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI4502187, Length=249, Percent_Identity=35.3413654618474, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI22538420, Length=246, Percent_Identity=32.9268292682927, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI4502181, Length=206, Percent_Identity=34.4660194174757, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI4755125, Length=206, Percent_Identity=34.4660194174757, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI37694062, Length=245, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI310133356, Length=200, Percent_Identity=31, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI310114181, Length=200, Percent_Identity=31, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1790362, Length=235, Percent_Identity=36.5957446808511, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787101, Length=191, Percent_Identity=35.0785340314136, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994009, Length=229, Percent_Identity=34.4978165938865, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17544068, Length=246, Percent_Identity=35.7723577235772, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71992966, Length=234, Percent_Identity=31.1965811965812, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17533613, Length=239, Percent_Identity=30.1255230125523, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32564052, Length=239, Percent_Identity=30.1255230125523, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17531431, Length=260, Percent_Identity=31.1538461538462, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=260, Percent_Identity=31.1538461538462, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17558372, Length=238, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71992961, Length=230, Percent_Identity=29.1304347826087, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71993722, Length=195, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=36.4077669902913, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6322985, Length=320, Percent_Identity=26.5625, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652747, Length=239, Percent_Identity=32.6359832635983, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45551084, Length=239, Percent_Identity=32.6359832635983, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24762342, Length=252, Percent_Identity=27.3809523809524, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24762344, Length=243, Percent_Identity=32.0987654320988, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24762346, Length=243, Percent_Identity=32.0987654320988, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24762348, Length=243, Percent_Identity=32.0987654320988, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20130305, Length=243, Percent_Identity=32.0987654320988, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922828, Length=194, Percent_Identity=30.9278350515464, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550503, Length=241, Percent_Identity=26.9709543568465, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28396; Mature: 28265

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISG
CCHHHHHHHHHHHHHHHCCCHHHCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPALTIGLAFKGTFPWSDVPGYIVAQMIGAIIGAVIVYLHYLPHWKETEDPGAKLGV
CCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLNPFIVGFLIVSIGLSLGGTT
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGVFHQVVFEEKQNA
CCEECCHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHCHHHHHHHHHHHHHCCCCC
ALIYVVIATVIILALSYIAGKKNRNDTNSRKVA
EEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISG
CHHHHHHHHHHHHHHHCCCHHHCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPALTIGLAFKGTFPWSDVPGYIVAQMIGAIIGAVIVYLHYLPHWKETEDPGAKLGV
CCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLNPFIVGFLIVSIGLSLGGTT
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGVFHQVVFEEKQNA
CCEECCHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHCHHHHHHHHHHHHHCCCCC
ALIYVVIATVIILALSYIAGKKNRNDTNSRKVA
EEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]