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The map label for this gene is ligA [H]

Identifier: 152974127

GI number: 152974127

Start: 328785

End: 330794

Strand: Direct

Name: ligA [H]

Synonym: Bcer98_0286

Alternate gene names: 152974127

Gene position: 328785-330794 (Clockwise)

Preceding gene: 152974126

Following gene: 152974128

Centisome position: 8.04

GC content: 38.46

Gene sequence:

>2010_bases
ATGTCAAAAGAGGCGGTACAACAGCGTATAGAAGAACTTCGTGATCTTTTAAATACATTTAATTATCAATATCACGTATT
AGACAATCCTTCTGTTTCTGATGCGGAATATGACCGCAATATGCAGGAGCTTATAAAATTAGAAACAGAAAACCCAGAAT
TTATAACGGAAGACTCTCCCTCTGTTCGTGTTGGGGGAGCTGTCCTTGATATATTTGAAAAGGTAACGCATAAATCACCG
ATGTTAAGTTTAGGAAATGCATTTAATGAAGGGGATTTACGTGATTTTGATCGCAGAGTTCGTCAAGGAATTGATGGTGT
AAATGTAAGATATATTTGTGAATTGAAAATTGACGGCCTTGCAGTTTCTCTTCACTATGAAAAAGGCCGCTTTATTCAAG
GTTCAACACGAGGCGATGGTATTACAGGCGAAGATATTACACAAAATTTAAAAACGATTAAGGCGATTCCTTTACGTTTG
CAAGAAGAAGTAACGTTAGAAGTGCGAGGCGAGGCTTATATGCCGAAGCGTTCATTTGTAAAATTAAATGAAGAGAAAGA
GCAAAATGGCGAAGCTGTATTTGCGAATCCTCGTAACGCAGCAGCAGGGTCTTTACGTCAACTGGATCCAAAAATCGCAG
CAAAACGTAACTTATCTATGTTTGTATATGGACTAGCTGATATAGAAGGAAAAACAATTACATCTCATAGTGAAGCACTA
GATTTCTTAGGAGAGCTAGGGTTTAAAACAAATCCGAATCGTCGCATCTGTGAGACAATTGAAGAAGTAATTGCTTATGT
AGAAGAATGGCAAGAAAAACGTCCAAATCTTGATTATGAAATTGACGGTATCGTAATTAAAGTAGATGATGTAACATTGC
AAGAACGTTTAGGAACGACTGCGAAGAGTCCAAGGTGGGCAATCGCTTATAAATTCCCAGCTGAAGAAGTTGTAACACGA
TTAACTGGTATCGAACTAAGTGTAGGACGTACAGGGGTTGTAACACCAACTGCAGAACTTGAACCAGTGCGAGTAGCTGG
GACGATTGTTCGCCGTGCTTCTTTACATAATGAAGATTTAATTCGGGAAAAAGACATTCGCATTGGCGATTATGTCGTTG
TGAAGAAGGCGGGCGATATTATTCCTGAGGTTGTGAATGTTGTTTTCGATAAACGAACTGGTAAAGAAGAAGCATACCAT
ATGCCAACGCACTGTCCTACTTGTGATAGTGGGCTTGTCCGCTTAGAAGAAGAGGTGGCTCTTCGCTGCATTAATCCAGC
TTGTCCAGCTCAAATTCGTGAAGGATTAATCCACTTTGTATCACGTAATGCGATGAATATTGATGGGCTAGGAGAACGCG
TTATCACACAGCTATTTGAAGCAAATTATATTCGAACATTTGCGGACCTATATGGTTTAACGAAAGATCAGTTACTCCAG
CTTGATCGCTTTGGTGAAAAATCAGCTTCTAATTTAGTACAAGCAATTGAAGCTTCGAAAGAAAACTCATTAGAACGATT
GTTATTTGGATTAGGTATTCGCCATGTTGGAGCAAAAGCAGCACGAACATTAGCTGAACATTTTGAAACGATGGATAATC
TTGTGAAAGCTGGGGAAGAAGAATTAAAGGCGATTAATGAGATCGGTGAAAAAATGGCGCAATCTATTGTTACGTACTTT
GATAATGAAGATGTACTACACTTAATTCAACAGTTTAAAGACTATGGCGTTAATATGACATATAAAGGTATAAAGCGTGC
TGATTTGCAAAATATCGCATCTTACTTTGCTGGAAAAACAGTTGTATTAACAGGTAAACTAGAAGTTATGGGACGAAGTG
AAGCAAAGAAAAAAATTGAAGCATTAGGCGGAAAAGTAACGGGTAGTGTAAGTAAAAGTACTGACCTTGTCATAGCAGGT
GAAGCGGCTGGATCGAAATTAGCACAGGCAGAGAAACATAATATTGAAATTTGGAATGAAGAGAGGTTCTTACAAGAGCT
GAATAAGTAA

Upstream 100 bases:

>100_bases
TGCAAAAGAATTAGATATTGCATTCCCAAGTCCGATTGGAATTAAACGATTATTAGCGAAATTTGCACCTGTGACGAAAC
AATAAGAAAGGAATGAGGAT

Downstream 100 bases:

>100_bases
GAGGTGCACTTACCATGAAAAAAATAGCATTAGCGGTATTGAGTCTTGGGCTACTTGTAAGTGGTTGTAGCACGGGACTA
AAAAAAGAAGAGAAGATCGT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSKEAVQQRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLETENPEFITEDSPSVRVGGAVLDIFEKVTHKSP
MLSLGNAFNEGDLRDFDRRVRQGIDGVNVRYICELKIDGLAVSLHYEKGRFIQGSTRGDGITGEDITQNLKTIKAIPLRL
QEEVTLEVRGEAYMPKRSFVKLNEEKEQNGEAVFANPRNAAAGSLRQLDPKIAAKRNLSMFVYGLADIEGKTITSHSEAL
DFLGELGFKTNPNRRICETIEEVIAYVEEWQEKRPNLDYEIDGIVIKVDDVTLQERLGTTAKSPRWAIAYKFPAEEVVTR
LTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVVFDKRTGKEEAYH
MPTHCPTCDSGLVRLEEEVALRCINPACPAQIREGLIHFVSRNAMNIDGLGERVITQLFEANYIRTFADLYGLTKDQLLQ
LDRFGEKSASNLVQAIEASKENSLERLLFGLGIRHVGAKAARTLAEHFETMDNLVKAGEEELKAINEIGEKMAQSIVTYF
DNEDVLHLIQQFKDYGVNMTYKGIKRADLQNIASYFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVIAG
EAAGSKLAQAEKHNIEIWNEERFLQELNK

Sequences:

>Translated_669_residues
MSKEAVQQRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLETENPEFITEDSPSVRVGGAVLDIFEKVTHKSP
MLSLGNAFNEGDLRDFDRRVRQGIDGVNVRYICELKIDGLAVSLHYEKGRFIQGSTRGDGITGEDITQNLKTIKAIPLRL
QEEVTLEVRGEAYMPKRSFVKLNEEKEQNGEAVFANPRNAAAGSLRQLDPKIAAKRNLSMFVYGLADIEGKTITSHSEAL
DFLGELGFKTNPNRRICETIEEVIAYVEEWQEKRPNLDYEIDGIVIKVDDVTLQERLGTTAKSPRWAIAYKFPAEEVVTR
LTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVVFDKRTGKEEAYH
MPTHCPTCDSGLVRLEEEVALRCINPACPAQIREGLIHFVSRNAMNIDGLGERVITQLFEANYIRTFADLYGLTKDQLLQ
LDRFGEKSASNLVQAIEASKENSLERLLFGLGIRHVGAKAARTLAEHFETMDNLVKAGEEELKAINEIGEKMAQSIVTYF
DNEDVLHLIQQFKDYGVNMTYKGIKRADLQNIASYFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVIAG
EAAGSKLAQAEKHNIEIWNEERFLQELNK
>Mature_668_residues
SKEAVQQRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLETENPEFITEDSPSVRVGGAVLDIFEKVTHKSPM
LSLGNAFNEGDLRDFDRRVRQGIDGVNVRYICELKIDGLAVSLHYEKGRFIQGSTRGDGITGEDITQNLKTIKAIPLRLQ
EEVTLEVRGEAYMPKRSFVKLNEEKEQNGEAVFANPRNAAAGSLRQLDPKIAAKRNLSMFVYGLADIEGKTITSHSEALD
FLGELGFKTNPNRRICETIEEVIAYVEEWQEKRPNLDYEIDGIVIKVDDVTLQERLGTTAKSPRWAIAYKFPAEEVVTRL
TGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVVFDKRTGKEEAYHM
PTHCPTCDSGLVRLEEEVALRCINPACPAQIREGLIHFVSRNAMNIDGLGERVITQLFEANYIRTFADLYGLTKDQLLQL
DRFGEKSASNLVQAIEASKENSLERLLFGLGIRHVGAKAARTLAEHFETMDNLVKAGEEELKAINEIGEKMAQSIVTYFD
NEDVLHLIQQFKDYGVNMTYKGIKRADLQNIASYFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVIAGE
AAGSKLAQAEKHNIEIWNEERFLQELNK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=670, Percent_Identity=48.2089552238806, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI87082305, Length=458, Percent_Identity=23.3624454148472, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74935; Mature: 74804

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEAVQQRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLETENPEFITEDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCHHHHHEECCCCCCEEECCCC
SVRVGGAVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDGVNVRYICELKIDGL
CEEECHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEECCE
AVSLHYEKGRFIQGSTRGDGITGEDITQNLKTIKAIPLRLQEEVTLEVRGEAYMPKRSFV
EEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCEECCEEEEEECCCCCCCCHHHE
KLNEEKEQNGEAVFANPRNAAAGSLRQLDPKIAAKRNLSMFVYGLADIEGKTITSHSEAL
ECCCHHCCCCCEEEECCCCCCCCCHHHCCHHHHHHCCCEEEEEEEECCCCCEECCHHHHH
DFLGELGFKTNPNRRICETIEEVIAYVEEWQEKRPNLDYEIDGIVIKVDDVTLQERLGTT
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCCCHHHHCCCC
AKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDL
CCCCCEEEEEECCHHHHHHHHHCCEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCHHH
IREKDIRIGDYVVVKKAGDIIPEVVNVVFDKRTGKEEAYHMPTHCPTCDSGLVRLEEEVA
HHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LRCINPACPAQIREGLIHFVSRNAMNIDGLGERVITQLFEANYIRTFADLYGLTKDQLLQ
HHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LDRFGEKSASNLVQAIEASKENSLERLLFGLGIRHVGAKAARTLAEHFETMDNLVKAGEE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELKAINEIGEKMAQSIVTYFDNEDVLHLIQQFKDYGVNMTYKGIKRADLQNIASYFAGKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCE
VVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVIAGEAAGSKLAQAEKHNIEIWNE
EEEEEEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEEECCCCCHHHHHHHCCCEEECH
ERFLQELNK
HHHHHHHCC
>Mature Secondary Structure 
SKEAVQQRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLETENPEFITEDSP
CHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCHHHHHEECCCCCCEEECCCC
SVRVGGAVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDGVNVRYICELKIDGL
CEEECHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEECCE
AVSLHYEKGRFIQGSTRGDGITGEDITQNLKTIKAIPLRLQEEVTLEVRGEAYMPKRSFV
EEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCEECCEEEEEECCCCCCCCHHHE
KLNEEKEQNGEAVFANPRNAAAGSLRQLDPKIAAKRNLSMFVYGLADIEGKTITSHSEAL
ECCCHHCCCCCEEEECCCCCCCCCHHHCCHHHHHHCCCEEEEEEEECCCCCEECCHHHHH
DFLGELGFKTNPNRRICETIEEVIAYVEEWQEKRPNLDYEIDGIVIKVDDVTLQERLGTT
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCCCHHHHCCCC
AKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDL
CCCCCEEEEEECCHHHHHHHHHCCEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCHHH
IREKDIRIGDYVVVKKAGDIIPEVVNVVFDKRTGKEEAYHMPTHCPTCDSGLVRLEEEVA
HHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LRCINPACPAQIREGLIHFVSRNAMNIDGLGERVITQLFEANYIRTFADLYGLTKDQLLQ
HHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LDRFGEKSASNLVQAIEASKENSLERLLFGLGIRHVGAKAARTLAEHFETMDNLVKAGEE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELKAINEIGEKMAQSIVTYFDNEDVLHLIQQFKDYGVNMTYKGIKRADLQNIASYFAGKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCE
VVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVIAGEAAGSKLAQAEKHNIEIWNE
EEEEEEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEEECCCCCHHHHHHHCCCEEECH
ERFLQELNK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA