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The map label for this gene is pcrA [H]

Identifier: 152974126

GI number: 152974126

Start: 326544

End: 328769

Strand: Direct

Name: pcrA [H]

Synonym: Bcer98_0285

Alternate gene names: 152974126

Gene position: 326544-328769 (Clockwise)

Preceding gene: 152974125

Following gene: 152974127

Centisome position: 7.99

GC content: 37.51

Gene sequence:

>2226_bases
ATGAGTATGACAGATCGATTATTGAACGGTTTAAACCCAGAACAGCAAAAAGCGGTACAAACAACAGAAGGCCCCCTTTT
ATTAATGGCGGGTGCAGGTAGTGGTAAAACACGCGTGTTAACGCACCGTATTGCATACTTACTTGGTGAGAAGGGGGTTG
CACCGTGGAACATACTAGCAATTACCTTCACAAATAAGGCAGCTCGTGAAATGCGTGAACGTATCGATACACTTGTTGGA
CCTGAAGCAGAAGATATTTGGATTTCTACATTCCACTCTATGTGTGTACGCATTTTACGACGTGACATTGATCGTATTGG
AATTAATCGAAACTTCACTATTTTAGATGCAAGCGATCAACTTACTGTTGTAAAAAAGATTATGAAAGAACGTAATATTG
ATCCGAAGAAATTCGATCCACGTTCTATTTTAGCTCGTATTAGTAATGCGAAAAATGAATTGTTATCTGCAGAGAAGTAT
GCAAAACAAATTTCAATTGCGGATCCATTTGAAAAATTATCAAGCGATGTGTATACAGAATATCAGAAGCGTCTTTTGAA
AAATAATTCATTGGATTTTGATGATTTAATTATGACAACGATTCACCTGTTTGAGCGCGTACCTGAGGTATTGGAATTTT
ATCAACGTAAGTTTCAATATATTCATGTGGACGAGTATCAAGATACGAACAGAGCGCAATATATGCTTGTAAATAAATTG
GCAGCGCGCTTTAAAAATCTTTGTGTTGTTGGTGATTCTGATCAGTCAATTTATCGTTGGCGTGGTGCGGATATTTCCAA
CATTCTTTCATTTGAAAAGGACTATGAGAACGCAAAGGTTATTTTGTTAGAACAAAACTACCGTTCCACAAAAAATATTT
TAAATGCAGCGAATGCCGTTATTGAGAACAATATAAATCGTAAGCCAAAGAAATTATGGACAGAAAATCAAATTGGAAGC
AAAATTTCATATTATCGAGCTGCAACGGAAAAAGATGAAGCATACTTTGTTGCGAAGAAAATTCGCGATGAAGTTCAAAT
GGGAAACAGAAAGTATACAGATTTTGCTGTATTATATCGTACGAATGCCCAATCTCGTATGGTCGAGGAGATTTTCTTGA
AATCAAATATCCCATACAAAATTGTTGGCGGAATTAAGTTCTATGACCGTAAAGAAATTAAAGATATTTTGGCATATTTA
CGTCTTATTGCGAATCCGGATGATGAAATTAGCTTTGCGCGCATTATTAATGTGCCAAAACGCGGAATTGGTGCCACTTC
CATTGATAAGATTATTAATTACGGTGTGCAAAATGGAATTTCATTAACAACTGTATTAGATGAAATTGAACATGTTGGTG
TGAGTGCGAAAGTTACAAAGGCTGTGAAAGAGTTTGCGGGTTTATTACACAATTGGGTAAACATGCAAGAATATTTATCA
GTTACAGAATTAGTAGAAGAAGTAATTGAAAAAACGGGTTATCGCGACATGCTTAAGAATGAGCGAACTTTAGAATCTGA
AGGCCGCTTAGAAAACTTAGACGAGTTTTTATCTGTTACACAAACATTTGAATCACAAAGTGAAGATAAGAGTTTAGTTG
CGTTCTTAACAGACTTAGCACTTGTTGCAGATATCGATCGAGTGGATGAAGACCCAACTGCTGGTGAAGAAGTGATTTTA
ATGACAATGCATTCAGCGAAAGGGTTAGAGTTTCCAGTTGTCTTCATTATTGGCTTAGAGGAAGGGGTCTTTCCGCATAC
TCGTTCGCTTATGGAAGAGGATGAAATGCAAGAAGAACGCCGTCTTGCGTATGTAGGGATTACACGTGCGGAAGAAGAGC
TATATTTATCGAATGCGCAAATGCGCACATTATTTGGTAGAACAAATATGAATGCAGCATCTCGATTTATTTCAGAAATT
CCGACAGAACTAATTGAACCATTAAATGAAACAAAACCAAAAAGAGAAACATTTGGTGCAAAAGCAAAAGTGGCAGCGAC
AACGACACGTTCTCGTTCGCAGATTGTAAGGCCAACTGTGAAAACAACAGGAGGAGAGCAAATTGGTTGGGCAGTAGGCG
ATAAAGCGCTCCATCAAAAGTGGGGAGTCGGTACGGTTGTCAGTGTAAAAGGAGAAGGTGATGCAAAAGAATTAGATATT
GCATTCCCAAGTCCGATTGGAATTAAACGATTATTAGCGAAATTTGCACCTGTGACGAAACAATAA

Upstream 100 bases:

>100_bases
ATATGCCGATACAGTTGTTGTTGGGAACGTCATTTATGAGAATATAAAAGCAGCGTTACAAACAGTAAAAGCTGTAAAAG
GAGAGTAGGTGCAAGTAAAT

Downstream 100 bases:

>100_bases
GAAAGGAATGAGGATATGTCAAAAGAGGCGGTACAACAGCGTATAGAAGAACTTCGTGATCTTTTAAATACATTTAATTA
TCAATATCACGTATTAGACA

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 741; Mature: 740

Protein sequence:

>741_residues
MSMTDRLLNGLNPEQQKAVQTTEGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNILAITFTNKAAREMRERIDTLVG
PEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDASDQLTVVKKIMKERNIDPKKFDPRSILARISNAKNELLSAEKY
AKQISIADPFEKLSSDVYTEYQKRLLKNNSLDFDDLIMTTIHLFERVPEVLEFYQRKFQYIHVDEYQDTNRAQYMLVNKL
AARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAKVILLEQNYRSTKNILNAANAVIENNINRKPKKLWTENQIGS
KISYYRAATEKDEAYFVAKKIRDEVQMGNRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGIKFYDRKEIKDILAYL
RLIANPDDEISFARIINVPKRGIGATSIDKIINYGVQNGISLTTVLDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQEYLS
VTELVEEVIEKTGYRDMLKNERTLESEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGEEVIL
MTMHSAKGLEFPVVFIIGLEEGVFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTNMNAASRFISEI
PTELIEPLNETKPKRETFGAKAKVAATTTRSRSQIVRPTVKTTGGEQIGWAVGDKALHQKWGVGTVVSVKGEGDAKELDI
AFPSPIGIKRLLAKFAPVTKQ

Sequences:

>Translated_741_residues
MSMTDRLLNGLNPEQQKAVQTTEGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNILAITFTNKAAREMRERIDTLVG
PEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDASDQLTVVKKIMKERNIDPKKFDPRSILARISNAKNELLSAEKY
AKQISIADPFEKLSSDVYTEYQKRLLKNNSLDFDDLIMTTIHLFERVPEVLEFYQRKFQYIHVDEYQDTNRAQYMLVNKL
AARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAKVILLEQNYRSTKNILNAANAVIENNINRKPKKLWTENQIGS
KISYYRAATEKDEAYFVAKKIRDEVQMGNRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGIKFYDRKEIKDILAYL
RLIANPDDEISFARIINVPKRGIGATSIDKIINYGVQNGISLTTVLDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQEYLS
VTELVEEVIEKTGYRDMLKNERTLESEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGEEVIL
MTMHSAKGLEFPVVFIIGLEEGVFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTNMNAASRFISEI
PTELIEPLNETKPKRETFGAKAKVAATTTRSRSQIVRPTVKTTGGEQIGWAVGDKALHQKWGVGTVVSVKGEGDAKELDI
AFPSPIGIKRLLAKFAPVTKQ
>Mature_740_residues
SMTDRLLNGLNPEQQKAVQTTEGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNILAITFTNKAAREMRERIDTLVGP
EAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDASDQLTVVKKIMKERNIDPKKFDPRSILARISNAKNELLSAEKYA
KQISIADPFEKLSSDVYTEYQKRLLKNNSLDFDDLIMTTIHLFERVPEVLEFYQRKFQYIHVDEYQDTNRAQYMLVNKLA
ARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAKVILLEQNYRSTKNILNAANAVIENNINRKPKKLWTENQIGSK
ISYYRAATEKDEAYFVAKKIRDEVQMGNRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGIKFYDRKEIKDILAYLR
LIANPDDEISFARIINVPKRGIGATSIDKIINYGVQNGISLTTVLDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQEYLSV
TELVEEVIEKTGYRDMLKNERTLESEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGEEVILM
TMHSAKGLEFPVVFIIGLEEGVFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTNMNAASRFISEIP
TELIEPLNETKPKRETFGAKAKVAATTTRSRSQIVRPTVKTTGGEQIGWAVGDKALHQKWGVGTVVSVKGEGDAKELDIA
FPSPIGIKRLLAKFAPVTKQ

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=736, Percent_Identity=40.2173913043478, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI48994965, Length=642, Percent_Identity=39.0965732087227, Blast_Score=417, Evalue=1e-117,
Organism=Escherichia coli, GI1787196, Length=347, Percent_Identity=28.2420749279539, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=771, Percent_Identity=29.4422827496757, Blast_Score=236, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6324477, Length=688, Percent_Identity=22.8197674418605, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 84265; Mature: 84133

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTDRLLNGLNPEQQKAVQTTEGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNILA
CCHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
ITFTNKAAREMRERIDTLVGPEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDASDQ
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
LTVVKKIMKERNIDPKKFDPRSILARISNAKNELLSAEKYAKQISIADPFEKLSSDVYTE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YQKRLLKNNSLDFDDLIMTTIHLFERVPEVLEFYQRKFQYIHVDEYQDTNRAQYMLVNKL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHH
AARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAKVILLEQNYRSTKNILNAANAV
HHHHHCEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHH
IENNINRKPKKLWTENQIGSKISYYRAATEKDEAYFVAKKIRDEVQMGNRKYTDFAVLYR
HHCCCCCCHHHHCCCHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEE
TNAQSRMVEEIFLKSNIPYKIVGGIKFYDRKEIKDILAYLRLIANPDDEISFARIINVPK
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
RGIGATSIDKIINYGVQNGISLTTVLDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQEYLS
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHH
VTELVEEVIEKTGYRDMLKNERTLESEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLA
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LVADIDRVDEDPTAGEEVILMTMHSAKGLEFPVVFIIGLEEGVFPHTRSLMEEDEMQEER
HHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
RLAYVGITRAEEELYLSNAQMRTLFGRTNMNAASRFISEIPTELIEPLNETKPKRETFGA
CEEEEEEECCHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCC
KAKVAATTTRSRSQIVRPTVKTTGGEQIGWAVGDKALHQKWGVGTVVSVKGEGDAKELDI
CEEEEEECCHHHHHHHCCCHHCCCCCCCCHHHCHHHHHHHCCCCEEEEEECCCCCCEEEE
AFPSPIGIKRLLAKFAPVTKQ
ECCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SMTDRLLNGLNPEQQKAVQTTEGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNILA
CHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
ITFTNKAAREMRERIDTLVGPEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDASDQ
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
LTVVKKIMKERNIDPKKFDPRSILARISNAKNELLSAEKYAKQISIADPFEKLSSDVYTE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YQKRLLKNNSLDFDDLIMTTIHLFERVPEVLEFYQRKFQYIHVDEYQDTNRAQYMLVNKL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHH
AARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAKVILLEQNYRSTKNILNAANAV
HHHHHCEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHH
IENNINRKPKKLWTENQIGSKISYYRAATEKDEAYFVAKKIRDEVQMGNRKYTDFAVLYR
HHCCCCCCHHHHCCCHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEE
TNAQSRMVEEIFLKSNIPYKIVGGIKFYDRKEIKDILAYLRLIANPDDEISFARIINVPK
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
RGIGATSIDKIINYGVQNGISLTTVLDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQEYLS
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHH
VTELVEEVIEKTGYRDMLKNERTLESEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLA
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LVADIDRVDEDPTAGEEVILMTMHSAKGLEFPVVFIIGLEEGVFPHTRSLMEEDEMQEER
HHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
RLAYVGITRAEEELYLSNAQMRTLFGRTNMNAASRFISEIPTELIEPLNETKPKRETFGA
CEEEEEEECCHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCC
KAKVAATTTRSRSQIVRPTVKTTGGEQIGWAVGDKALHQKWGVGTVVSVKGEGDAKELDI
CEEEEEECCHHHHHHHCCCHHCCCCCCCCHHHCHHHHHHHCCCCEEEEEECCCCCCEEEE
AFPSPIGIKRLLAKFAPVTKQ
ECCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]