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The map label for this gene is yaaO [H]

Identifier: 152973877

GI number: 152973877

Start: 34766

End: 36184

Strand: Direct

Name: yaaO [H]

Synonym: Bcer98_0024

Alternate gene names: 152973877

Gene position: 34766-36184 (Clockwise)

Preceding gene: 152973876

Following gene: 152973878

Centisome position: 0.85

GC content: 36.08

Gene sequence:

>1419_bases
ATGAATCAAAATCAAATACCGTTATATGAAGCATTAGTACGCTTTAAACAACAACAACCGTTATCTTTACATGTTCCAGG
TCATAAAAATGGTTTGAATTTCCCTAAAGAAGCGATCGACAGCTTTAAAGATATACTTTCTATTGATGTAACTGAATTAA
CAGGATTAGATGATTTACATAGTCCCTCTGAATGTATAGACGAGGCACAACGATTGCTGGCTGATGTATATGAAGTGCAG
AAAAGTTACTTTTTAGTTAATGGCTCTACAGTTGGAAATTTAGCAATGGTTTTATCTTGCTGCGGGGAAGAGGACATTGT
TCTTGTACAAAGAAATTGTCATAAATCCATTATAAATGCTTTAAAGTTAGCAGGTGCTAATCCAGTATTTTTAGACCCGT
GGATTGATGAGGTATATCATGTGCCTGTTGGCGTCCATAATGAAACCATAAAAAAGGCTATTGACCAGTATCCAAATGCT
AAAGCACTTATTTTAACACACCCTAATTATTATGGTATGGGCGTCAATTTAAAAGAAAGTATCGCCTATGCGCATCAGCA
TCAAATACCTGTATTAGTAGATGAGGCACATGGGGCACATTTTTGTTTGGGGGAACCATTTCCTCAATCAGCGGTCGCGT
ATGGTGCGGACATTGTAGTGCAGTCTGCACATAAAACATTACCTGCAATGACGATGGGGTCTTATTTACATATAAATAGT
GATTTAATAAATGGAGAGAAAGTTTTTCGTTATTTAAATATGTTACAATCTAGTAGCCCATCCTATCCAATTATGGCTTC
TTTGGATATCGCTCGTTTTGCATTAGCAAATATGAAAGAAAAGGGTTATCATTCCATTATTGAGTTCATCAATCAATTTA
AAGAGGCATTACATTCTATTCCGCAAATAAAGATTTTGCAATATCCATTACAGGATGAACTCAAGGTTACTGTACAAAGT
CGTTGTCAATTGTCTGGGTATGAATTACAGTCCCTGTTTGAACAGGCTGGTATATATGCAGAAATGGCAGATCCGTATAA
CGTACTGTTTATTTTACCGTTACAAGTAAATGAGAAATACATGAAAGGAATAGAAACCATGCGATCACTGCTAAGCCATT
ATAAGATAACAGACAAGAGACCTTCTATTCGTTATACTTATAAAGGAGGGATTTCTCTTTTACCGTTTACATATAAACAT
TTAGAGGAATATGAGACGAAACGAGTACCTATAGAAGAAGCTGTTGGTATGATAGCAGCAGAGATGGTTATTCCATATCC
ACCTGGAATACCACTGATTATGTATGGGGAAACAATTCGCTTAGAGCATATAAGAGAGATGGCCCATTTAGAAAGAACTG
GAGCTCGTTTTCAAGGGAATCCAGCGTATATAAAGGTATATGTTATAGAGAGAAAGTAG

Upstream 100 bases:

>100_bases
AAGAAAATAGAAGTATCATATTTTTAAATAGGCATATGCCTATTATTTTTTTTGGTTTGAGTATAATAGATGGAGAAAGA
AAAGTTAGGAAGAGGTACAT

Downstream 100 bases:

>100_bases
GTTTTAGGATGAAGGGATTATTTGTAACAATTGAAGGACCAGAAGGGTCAGGAAAATCAACATTAATTACAAAGTTATTA
CCATATTTTGAAAATAAGGG

Product: Orn/Lys/Arg decarboxylase major region

Products: cadaverine; CO2

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNQNQIPLYEALVRFKQQQPLSLHVPGHKNGLNFPKEAIDSFKDILSIDVTELTGLDDLHSPSECIDEAQRLLADVYEVQ
KSYFLVNGSTVGNLAMVLSCCGEEDIVLVQRNCHKSIINALKLAGANPVFLDPWIDEVYHVPVGVHNETIKKAIDQYPNA
KALILTHPNYYGMGVNLKESIAYAHQHQIPVLVDEAHGAHFCLGEPFPQSAVAYGADIVVQSAHKTLPAMTMGSYLHINS
DLINGEKVFRYLNMLQSSSPSYPIMASLDIARFALANMKEKGYHSIIEFINQFKEALHSIPQIKILQYPLQDELKVTVQS
RCQLSGYELQSLFEQAGIYAEMADPYNVLFILPLQVNEKYMKGIETMRSLLSHYKITDKRPSIRYTYKGGISLLPFTYKH
LEEYETKRVPIEEAVGMIAAEMVIPYPPGIPLIMYGETIRLEHIREMAHLERTGARFQGNPAYIKVYVIERK

Sequences:

>Translated_472_residues
MNQNQIPLYEALVRFKQQQPLSLHVPGHKNGLNFPKEAIDSFKDILSIDVTELTGLDDLHSPSECIDEAQRLLADVYEVQ
KSYFLVNGSTVGNLAMVLSCCGEEDIVLVQRNCHKSIINALKLAGANPVFLDPWIDEVYHVPVGVHNETIKKAIDQYPNA
KALILTHPNYYGMGVNLKESIAYAHQHQIPVLVDEAHGAHFCLGEPFPQSAVAYGADIVVQSAHKTLPAMTMGSYLHINS
DLINGEKVFRYLNMLQSSSPSYPIMASLDIARFALANMKEKGYHSIIEFINQFKEALHSIPQIKILQYPLQDELKVTVQS
RCQLSGYELQSLFEQAGIYAEMADPYNVLFILPLQVNEKYMKGIETMRSLLSHYKITDKRPSIRYTYKGGISLLPFTYKH
LEEYETKRVPIEEAVGMIAAEMVIPYPPGIPLIMYGETIRLEHIREMAHLERTGARFQGNPAYIKVYVIERK
>Mature_472_residues
MNQNQIPLYEALVRFKQQQPLSLHVPGHKNGLNFPKEAIDSFKDILSIDVTELTGLDDLHSPSECIDEAQRLLADVYEVQ
KSYFLVNGSTVGNLAMVLSCCGEEDIVLVQRNCHKSIINALKLAGANPVFLDPWIDEVYHVPVGVHNETIKKAIDQYPNA
KALILTHPNYYGMGVNLKESIAYAHQHQIPVLVDEAHGAHFCLGEPFPQSAVAYGADIVVQSAHKTLPAMTMGSYLHINS
DLINGEKVFRYLNMLQSSSPSYPIMASLDIARFALANMKEKGYHSIIEFINQFKEALHSIPQIKILQYPLQDELKVTVQS
RCQLSGYELQSLFEQAGIYAEMADPYNVLFILPLQVNEKYMKGIETMRSLLSHYKITDKRPSIRYTYKGGISLLPFTYKH
LEEYETKRVPIEEAVGMIAAEMVIPYPPGIPLIMYGETIRLEHIREMAHLERTGARFQGNPAYIKVYVIERK

Specific function: Appears To Play A Role In pH Homeostasis By Consuming Protons And Neutralizing The Acidic By-Products Of Carbohydrate Fermentation. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1790573, Length=552, Percent_Identity=22.2826086956522, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786384, Length=543, Percent_Identity=22.4677716390424, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI221142684, Length=299, Percent_Identity=28.0936454849498, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C [H]

EC number: 4.1.1.18

Molecular weight: Translated: 53352; Mature: 53352

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNQIPLYEALVRFKQQQPLSLHVPGHKNGLNFPKEAIDSFKDILSIDVTELTGLDDLH
CCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCC
SPSECIDEAQRLLADVYEVQKSYFLVNGSTVGNLAMVLSCCGEEDIVLVQRNCHKSIINA
CHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHH
LKLAGANPVFLDPWIDEVYHVPVGVHNETIKKAIDQYPNAKALILTHPNYYGMGVNLKES
HHHCCCCCEEECHHHHHHEECCCCCCHHHHHHHHHHCCCCCEEEEECCCEEECCCCHHHH
IAYAHQHQIPVLVDEAHGAHFCLGEPFPQSAVAYGADIVVQSAHKTLPAMTMGSYLHINS
HHHHHHCCCCEEEECCCCCEEECCCCCCCHHHHHCHHHEEEHHHHHCCHHHCCCEEEECC
DLINGEKVFRYLNMLQSSSPSYPIMASLDIARFALANMKEKGYHSIIEFINQFKEALHSI
CCCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
PQIKILQYPLQDELKVTVQSRCQLSGYELQSLFEQAGIYAEMADPYNVLFILPLQVNEKY
CCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCEEEEEEEECCCHHH
MKGIETMRSLLSHYKITDKRPSIRYTYKGGISLLPFTYKHLEEYETKRVPIEEAVGMIAA
HHHHHHHHHHHHHCEECCCCCCEEEEECCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHH
EMVIPYPPGIPLIMYGETIRLEHIREMAHLERTGARFQGNPAYIKVYVIERK
HHEECCCCCCCEEEECCEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MNQNQIPLYEALVRFKQQQPLSLHVPGHKNGLNFPKEAIDSFKDILSIDVTELTGLDDLH
CCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCC
SPSECIDEAQRLLADVYEVQKSYFLVNGSTVGNLAMVLSCCGEEDIVLVQRNCHKSIINA
CHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHH
LKLAGANPVFLDPWIDEVYHVPVGVHNETIKKAIDQYPNAKALILTHPNYYGMGVNLKES
HHHCCCCCEEECHHHHHHEECCCCCCHHHHHHHHHHCCCCCEEEEECCCEEECCCCHHHH
IAYAHQHQIPVLVDEAHGAHFCLGEPFPQSAVAYGADIVVQSAHKTLPAMTMGSYLHINS
HHHHHHCCCCEEEECCCCCEEECCCCCCCHHHHHCHHHEEEHHHHHCCHHHCCCEEEECC
DLINGEKVFRYLNMLQSSSPSYPIMASLDIARFALANMKEKGYHSIIEFINQFKEALHSI
CCCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
PQIKILQYPLQDELKVTVQSRCQLSGYELQSLFEQAGIYAEMADPYNVLFILPLQVNEKY
CCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCEEEEEEEECCCHHH
MKGIETMRSLLSHYKITDKRPSIRYTYKGGISLLPFTYKHLEEYETKRVPIEEAVGMIAA
HHHHHHHHHHHHHCEECCCCCCEEEEECCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHH
EMVIPYPPGIPLIMYGETIRLEHIREMAHLERTGARFQGNPAYIKVYVIERK
HHEECCCCCCCEEEECCEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate; Sulfhydryl groups [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.4 {S-aminoethyl-L-Cys}} 2 {L-Lys}} 1.5 {L-Lys}} 7 {delta-hydroxylysine}} [C]

Substrates: L-lysine

Specific reaction: L-lysine = cadaverine + CO2

General reaction: Decarboxylation [C]

Inhibitor: NaBH4; NaCl [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]