Click here to switch to the map view.

The map label for this gene is pdxT

Identifier: 152973865

GI number: 152973865

Start: 19574

End: 20164

Strand: Direct

Name: pdxT

Synonym: Bcer98_0011

Alternate gene names: 152973865

Gene position: 19574-20164 (Clockwise)

Preceding gene: 152973864

Following gene: 152973866

Centisome position: 0.48

GC content: 39.09

Gene sequence:

>591_bases
ATGGTAAAAATCGGTGTGTTAGGTCTTCAAGGAGCAGTTCGAGAGCATGTAAAAGCAATTGAAGCAAGCGGTGCAGAAGC
TGTAGTTGTAAAACAAGTAGAACAACTTCAAGAAATTGATGGGCTTATTTTACCAGGCGGTGAAAGTACAACAATGCGTC
GTCTCATTGATAAGTATCATTTTATGGAACCACTTCGTGAATTTGCGCGTTCTAGAAAGCCGATGTTTGGTACATGTGCA
GGTATGATTTTACTTGCAAATAAACTGATTGGTTATGAAGAAGCGCATATTGGTGCAATGGATATTACAGTAGAACGCAA
TGCATTTGGTCGTCAAAAGGATAGCTTCGAAGCTACACTTTCGATTAAAGGTGTAGGAGAAGATTTTACAGGTGTATTTA
TTCGAGCGCCGTATGTCGTTGATATAGCTGATGATGTAGAAGTTCTTTCTATGCACAATGGTCGAATGGTTGCAGTGAAA
CAAGGGCCATTTTTGGCTGCTTCTTTCCATCCAGAGTTAACAGATGATTATCGTGTTACAGCGTATTTTGTAGAGATGGT
AGAAGAAGCAAAAATGAAGAAAGTTGTATAA

Upstream 100 bases:

>100_bases
CTAAAGGATTAGGTGATGCAATGAAGGGCGTTGAAATTTCAACACTATTACCAGAACAACGTATGCAAGAACGCGGTTGG
TAATTAAAGGAGATTTAAAA

Downstream 100 bases:

>100_bases
GTAACTTGCAACTTGTGTAAGATTATAGTAAATTGATGGTAACAATTTTATAAAATTAGCGTGTTGATAGGAAATAGTAA
CAAATAGTGTTTCTTGACAG

Product: glutamine amidotransferase subunit PdxT

Products: AMP; diphosphate; GMP; L-glutamate

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MVKIGVLGLQGAVREHVKAIEASGAEAVVVKQVEQLQEIDGLILPGGESTTMRRLIDKYHFMEPLREFARSRKPMFGTCA
GMILLANKLIGYEEAHIGAMDITVERNAFGRQKDSFEATLSIKGVGEDFTGVFIRAPYVVDIADDVEVLSMHNGRMVAVK
QGPFLAASFHPELTDDYRVTAYFVEMVEEAKMKKVV

Sequences:

>Translated_196_residues
MVKIGVLGLQGAVREHVKAIEASGAEAVVVKQVEQLQEIDGLILPGGESTTMRRLIDKYHFMEPLREFARSRKPMFGTCA
GMILLANKLIGYEEAHIGAMDITVERNAFGRQKDSFEATLSIKGVGEDFTGVFIRAPYVVDIADDVEVLSMHNGRMVAVK
QGPFLAASFHPELTDDYRVTAYFVEMVEEAKMKKVV
>Mature_196_residues
MVKIGVLGLQGAVREHVKAIEASGAEAVVVKQVEQLQEIDGLILPGGESTTMRRLIDKYHFMEPLREFARSRKPMFGTCA
GMILLANKLIGYEEAHIGAMDITVERNAFGRQKDSFEATLSIKGVGEDFTGVFIRAPYVVDIADDVEVLSMHNGRMVAVK
QGPFLAASFHPELTDDYRVTAYFVEMVEEAKMKKVV

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323995, Length=210, Percent_Identity=36.6666666666667, Blast_Score=109, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323742, Length=207, Percent_Identity=37.1980676328502, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321048, Length=210, Percent_Identity=35.7142857142857, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_BACCN (A7GJS9)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001373382.1
- ProteinModelPortal:   A7GJS9
- SMR:   A7GJS9
- STRING:   A7GJS9
- EnsemblBacteria:   EBBACT00000036298
- GeneID:   5344989
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_0011
- eggNOG:   COG0311
- GeneTree:   EBGT00050000000582
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13525
- BioCyc:   BCER315749:BCER98_0011-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: 6.3.5.2

Molecular weight: Translated: 21694; Mature: 21694

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 79-79 ACT_SITE 170-170 ACT_SITE 172-172 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIGVLGLQGAVREHVKAIEASGAEAVVVKQVEQLQEIDGLILPGGESTTMRRLIDKYH
CEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHH
FMEPLREFARSRKPMFGTCAGMILLANKLIGYEEAHIGAMDITVERNAFGRQKDSFEATL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCEEEE
SIKGVGEDFTGVFIRAPYVVDIADDVEVLSMHNGRMVAVKQGPFLAASFHPELTDDYRVT
EEEECCCCCCEEEEECCEEEEECCCEEEEEECCCEEEEEECCCEEEEECCCCCCCCHHHH
AYFVEMVEEAKMKKVV
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKIGVLGLQGAVREHVKAIEASGAEAVVVKQVEQLQEIDGLILPGGESTTMRRLIDKYH
CEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHH
FMEPLREFARSRKPMFGTCAGMILLANKLIGYEEAHIGAMDITVERNAFGRQKDSFEATL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCEEEE
SIKGVGEDFTGVFIRAPYVVDIADDVEVLSMHNGRMVAVKQGPFLAASFHPELTDDYRVT
EEEECCCCCCEEEEECCEEEEECCCEEEEEECCCEEEEEECCCEEEEECCCCCCCCHHHH
AYFVEMVEEAKMKKVV
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xanthosine 5'-phosphate; L-glutamine; H2O

Specific reaction: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA