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The map label for this gene is gyrB [H]

Identifier: 152973859

GI number: 152973859

Start: 4698

End: 6620

Strand: Direct

Name: gyrB [H]

Synonym: Bcer98_0005

Alternate gene names: 152973859

Gene position: 4698-6620 (Clockwise)

Preceding gene: 152973858

Following gene: 152973860

Centisome position: 0.11

GC content: 38.12

Gene sequence:

>1923_bases
ATGGAACAAAAGGAAATGCAAGCAAACTCATATGATGAAAGTCAAATACAGGTACTTGAAGGGTTAGAAGCAGTTCGAAA
ACGCCCAGGTATGTACATTGGATCGACAAGTGGAAAAGGTCTTCATCACCTTGTATGGGAGATTGTAGATAATAGTATTG
ATGAAGCGTTAGCGGGATATTGTGATGAGATTAATGTGGTCATCGAAGAAGACGATAGTATCCGTGTAACGGATAACGGC
CGTGGTATTCCAGTTGGTATACAAGAAAAAATGGGACGTCCTGCAGTAGAAGTCATTATGACAGTACTTCATGCTGGTGG
TAAATTTGGCGGTGGTGGTTATAAAGTTTCCGGTGGTCTTCATGGTGTAGGGGCATCTGTAGTAAACGCCCTATCAACCG
AGTTAGAAGTATTTGTACACCGCGAAGGAAAAATCCACTACCAAAAATATGAACGAGGCATTCCAGTTGCAGATTTAAAA
GTAATTGGTGAAACAGATCGCACAGGAACAGTAACTCGCTTTAAGCCAGACCCAGAAATTTTTAAAGAAACAACCGTGTA
TGATTTTGATATATTAGCAACTCGTATGCGTGAACTAGCATTTTTAAATCGTAATATTAAATTAACAATTGAAGATAAAC
GTGAAAATAAACAGAAGAAAGAATTCCATTACGAAGGTGGAATTAAATCTTATGTTGAACATTTAAATCGTTCGAAGCAG
CCAATTCATGAAGAACCAGTATATGTAGAAGGCGCAAAAGATGGGATTCAGGTTGAAGTTTCTCTCCAATATAACGAAGG
ATATACAAATAATATTTATTCATTTACAAATAACATCCATACGTATGAAGGTGGTACGCATGAGGTTGGATTTAAAACAG
CTTTAACGCGTGTGATTAATGACTATGGAAGAAAAAATAATATTTTAAAAGATGCTGATAGTAATTTAACTGGTGAAGAT
GTTCGGGAAGGTTTAACTGCAATTGTATCCATTAAACATCCGAATCCGCAATTTGAAGGGCAGACAAAGACGAAACTTGG
TAATAGCGAAGCGAGAACGATTACAGAGTCTGTATTTTCAGAGGCGTTTGAAAAGTTTCTTCTAGAAAATCCAAATGTTG
CACGTAAAATTGTGGAAAAAGGGACAATGGCAGCAAGAGCACGTGTAGCTGCGAAGAAAGCGCGTGAATTAACTCGTAGA
AAGAGTGCGTTAGAGGTTTCAAGTTTACCTGGTAAGTTGGCGGATTGTTCTTCTAAAGACCCATCCATCAGTGAAATTTA
TATCGTAGAGGGTGACTCTGCGGGTGGATCTGCAAAGCAGGGGCGCGATCGTCACTTCCAAGCAATCTTACCGCTTAAGG
GGAAAATCATTAACGTTGAGAAAGCTCGATTAGATAAAATTTTATCCAATGATGAAGTGCGTACAATTATTACAGCAATT
GGGACAAACATCGGTGGCGATTTTGATATATCAAAGGCCCGCTATCATAAAGTTATTATTATGACCGATGCCGATGTCGA
TGGTGCACATATTCGTACCCTATTATTAACGTTCTTCTATCGTTATATGCGTCAAATTATTGAACATGGTTATGTATATA
TTGCACAACCACCATTGTTTAAAGTGCAACAAGGGAAAAAGATTCAGTATGCTTATAACGAAAAAGAACTTGAAAGAATA
TTGGCCGAGTTGCCAGCGCAACCTAAGCCAAGTATTCAGCGTTACAAAGGTCTTGGTGAGATGAATCCGGCTCAATTATG
GGAAACGACAATGGATCCAGAGGTGCGTTCATTATTGCAAGTTTCTTTACAAGATGCAATCGAAGCGGATGAAACTTTTG
AAATTTTAATGGGAGATAAAGTTGAACCGCGTCGTAATTTCATTCAAGAGAATGCAAAATATGTAAAAAACCTTGATATT
TAA

Upstream 100 bases:

>100_bases
AAAAAGCGAAAACAATTCATGTAAAAAGCGGCACGGTAGATTGTGAAATAGACAGAATATAACTTCTGTTTAAATGGAAA
AGTAGGTGATCTTTGTGTCA

Downstream 100 bases:

>100_bases
GATAAATAACGACAGGAATCTCAATGTTCCTGTCGTCTATATATAAATCAATGTAATTTGTAACGGAAATGTAAGAGGAG
GTGCTGGTTGATGTCAGATA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MEQKEMQANSYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCDEINVVIEEDDSIRVTDNG
RGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLK
VIGETDRTGTVTRFKPDPEIFKETTVYDFDILATRMRELAFLNRNIKLTIEDKRENKQKKEFHYEGGIKSYVEHLNRSKQ
PIHEEPVYVEGAKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVINDYGRKNNILKDADSNLTGED
VREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFSEAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRR
KSALEVSSLPGKLADCSSKDPSISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
GTNIGGDFDISKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIEHGYVYIAQPPLFKVQQGKKIQYAYNEKELERI
LAELPAQPKPSIQRYKGLGEMNPAQLWETTMDPEVRSLLQVSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI

Sequences:

>Translated_640_residues
MEQKEMQANSYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCDEINVVIEEDDSIRVTDNG
RGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLK
VIGETDRTGTVTRFKPDPEIFKETTVYDFDILATRMRELAFLNRNIKLTIEDKRENKQKKEFHYEGGIKSYVEHLNRSKQ
PIHEEPVYVEGAKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVINDYGRKNNILKDADSNLTGED
VREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFSEAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRR
KSALEVSSLPGKLADCSSKDPSISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
GTNIGGDFDISKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIEHGYVYIAQPPLFKVQQGKKIQYAYNEKELERI
LAELPAQPKPSIQRYKGLGEMNPAQLWETTMDPEVRSLLQVSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI
>Mature_640_residues
MEQKEMQANSYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCDEINVVIEEDDSIRVTDNG
RGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLK
VIGETDRTGTVTRFKPDPEIFKETTVYDFDILATRMRELAFLNRNIKLTIEDKRENKQKKEFHYEGGIKSYVEHLNRSKQ
PIHEEPVYVEGAKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVINDYGRKNNILKDADSNLTGED
VREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFSEAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRR
KSALEVSSLPGKLADCSSKDPSISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
GTNIGGDFDISKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIEHGYVYIAQPPLFKVQQGKKIQYAYNEKELERI
LAELPAQPKPSIQRYKGLGEMNPAQLWETTMDPEVRSLLQVSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=610, Percent_Identity=26.2295081967213, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI19913408, Length=632, Percent_Identity=26.4240506329114, Blast_Score=131, Evalue=3e-30,
Organism=Escherichia coli, GI48994957, Length=554, Percent_Identity=59.3862815884477, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=628, Percent_Identity=40.4458598726115, Blast_Score=457, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17535065, Length=657, Percent_Identity=24.8097412480974, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=541, Percent_Identity=25.1386321626617, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=227, Percent_Identity=26.8722466960352, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=624, Percent_Identity=26.1217948717949, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17136538, Length=623, Percent_Identity=25.8426966292135, Blast_Score=117, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71856; Mature: 71856

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKEMQANSYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGY
CCCHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
CDEINVVIEEDDSIRVTDNGRGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGL
CCCCEEEEECCCCEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLKVIGETDRTGTVTRFKPDPEI
CHHHHHHHHHHHHHEEEEEECCCCEEHHHHHCCCCEEEEEEECCCCCCCCEEEECCCHHH
FKETTVYDFDILATRMRELAFLNRNIKLTIEDKRENKQKKEFHYEGGIKSYVEHLNRSKQ
HHHCCEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCCCC
PIHEEPVYVEGAKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVIN
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHCCCEEECCCCCCCCHHHHHHHHHHH
DYGRKNNILKDADSNLTGEDVREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFS
HHCCCCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHH
EAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRRKSALEVSSLPGKLADCSSKD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
PSISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
CCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHCCCHHHHHHHHH
GTNIGGDFDISKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIEHGYVYIAQPPLF
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCE
KVQQGKKIQYAYNEKELERILAELPAQPKPSIQRYKGLGEMNPAQLWETTMDPEVRSLLQ
ECCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHCCCCHHHHHHHH
VSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEQKEMQANSYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGY
CCCHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
CDEINVVIEEDDSIRVTDNGRGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGL
CCCCEEEEECCCCEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLKVIGETDRTGTVTRFKPDPEI
CHHHHHHHHHHHHHEEEEEECCCCEEHHHHHCCCCEEEEEEECCCCCCCCEEEECCCHHH
FKETTVYDFDILATRMRELAFLNRNIKLTIEDKRENKQKKEFHYEGGIKSYVEHLNRSKQ
HHHCCEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCCCC
PIHEEPVYVEGAKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVIN
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHCCCEEECCCCCCCCHHHHHHHHHHH
DYGRKNNILKDADSNLTGEDVREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFS
HHCCCCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHH
EAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRRKSALEVSSLPGKLADCSSKD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
PSISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
CCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHCCCHHHHHHHHH
GTNIGGDFDISKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIEHGYVYIAQPPLF
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCE
KVQQGKKIQYAYNEKELERILAELPAQPKPSIQRYKGLGEMNPAQLWETTMDPEVRSLLQ
ECCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHCCCCHHHHHHHH
VSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629; 10103241 [H]