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The map label for this gene is recF [H]

Identifier: 152973858

GI number: 152973858

Start: 3538

End: 4659

Strand: Direct

Name: recF [H]

Synonym: Bcer98_0004

Alternate gene names: 152973858

Gene position: 3538-4659 (Clockwise)

Preceding gene: 152973857

Following gene: 152973859

Centisome position: 0.09

GC content: 33.24

Gene sequence:

>1122_bases
TTGTTTATTTCAGAAATACAATTAAAAAATTATCGAAATTATGAAGATTTAAACCTTTCTTTTGAGGATAAAGTCAATGT
AATCATTGGTGAAAATGCACAAGGGAAAACAAATTTGATGGAAGCGATCTATGTGTTAGCAATGGCGAAATCTCATAGAA
CTTCAAATGATCGTGAACTCATCCGATGGGATGAGGATTATGGTAAAATAAAAGGTAGATTACAAAAACGAAATAGTTCC
GTATCTTTGGAATTAAATATTTCTAAAAAAGGAAAAAAGGCAAAATTGAATGAACTTGAACAACAAAAGTTGAGCCAATA
TATTGGTGAGATGAACGTTGTTATGTTTGCCCCTGAAGATTTAAATCTTGTAAAAGGAAGTCCTCAAGTCAGAAGACGCT
TTTTAGATATGGAATTAGGACAAATAGCTCCTGTTTATTTGTATGAATTAAGTCAATATCAAAAAGTGCTCACACAACGA
AACCATTTGCTTAAGGCAATGCAAGGTAAAAATGAGGAAACGATGTTGGATGTATTTACGCTTCAACTTATTGAACATGG
TACGAAAATTCTACAAAAACGTTTTGAATTCTTACATTTACTACAAGAGTGGGCTGCTCCAATCCATCGTGGGATTAGCA
GAGGGCTTGAGGAATTGGAAATCGTTTATAAGCCAAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATAAAAGAA
GTATACTATGAAAGTTTTCAATCTGTGAAACAACGTGAAATTTTCCGTGGTACGACTTTAATTGGTCCTCATCGTGATGA
TTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGCTCACAAGGCCAGCAACGAACAACTGCACTATCCCTAA
AATTAGCTGAAATTGAATTGATTTATGCAGAGGTAAAAGAGTACCCAATTCTTTTATTAGATGATGTATTATCAGAATTA
GATGATTACCGTCAATCACATCTTTTAAATACAATTCAAGGAAAAGTGCAAACATTTGTGACAACGACGAGTGTCGACGG
AATTGAACACGAAACATTAAAAAAAGCGAAAACAATTCATGTAAAAAGCGGCACGGTAGATTGTGAAATAGACAGAATAT
AA

Upstream 100 bases:

>100_bases
AGAACTTGAAAATAGACGAGGTAGAAAATTGTACGCCAATGATATAGTTGAAATTCCGGGAAACGGAACATTTCAAGTTC
AATCATAAAGGGGGAGCCCT

Downstream 100 bases:

>100_bases
CTTCTGTTTAAATGGAAAAGTAGGTGATCTTTGTGTCAATGGAACAAAAGGAAATGCAAGCAAACTCATATGATGAAAGT
CAAATACAGGTACTTGAAGG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MFISEIQLKNYRNYEDLNLSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDYGKIKGRLQKRNSS
VSLELNISKKGKKAKLNELEQQKLSQYIGEMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKAMQGKNEETMLDVFTLQLIEHGTKILQKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKIKE
VYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYAEVKEYPILLLDDVLSEL
DDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKKAKTIHVKSGTVDCEIDRI

Sequences:

>Translated_373_residues
MFISEIQLKNYRNYEDLNLSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDYGKIKGRLQKRNSS
VSLELNISKKGKKAKLNELEQQKLSQYIGEMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKAMQGKNEETMLDVFTLQLIEHGTKILQKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKIKE
VYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYAEVKEYPILLLDDVLSEL
DDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKKAKTIHVKSGTVDCEIDRI
>Mature_373_residues
MFISEIQLKNYRNYEDLNLSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDYGKIKGRLQKRNSS
VSLELNISKKGKKAKLNELEQQKLSQYIGEMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKAMQGKNEETMLDVFTLQLIEHGTKILQKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKIKE
VYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYAEVKEYPILLLDDVLSEL
DDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKKAKTIHVKSGTVDCEIDRI

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=369, Percent_Identity=24.9322493224932, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 43135; Mature: 43135

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFISEIQLKNYRNYEDLNLSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDREL
CCCCCCHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
IRWDEDYGKIKGRLQKRNSSVSLELNISKKGKKAKLNELEQQKLSQYIGEMNVVMFAPED
EEECHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQRNHLLKAMQGKNEETMLDVFT
CCEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LQLIEHGTKILQKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKIKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCCCHHHHHH
VYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIEL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCHHHHEEEHHHHHH
IYAEVKEYPILLLDDVLSELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKKAKTIH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHEEE
VKSGTVDCEIDRI
EECCCEEEEECCC
>Mature Secondary Structure
MFISEIQLKNYRNYEDLNLSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDREL
CCCCCCHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
IRWDEDYGKIKGRLQKRNSSVSLELNISKKGKKAKLNELEQQKLSQYIGEMNVVMFAPED
EEECHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQRNHLLKAMQGKNEETMLDVFT
CCEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LQLIEHGTKILQKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKIKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCCCHHHHHH
VYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIEL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCHHHHEEEHHHHHH
IYAEVKEYPILLLDDVLSELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKKAKTIH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHEEE
VKSGTVDCEIDRI
EECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA