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The map label for this gene is ascB [H]

Identifier: 152971574

GI number: 152971574

Start: 3348753

End: 3350177

Strand: Direct

Name: ascB [H]

Synonym: KPN_03052

Alternate gene names: 152971574

Gene position: 3348753-3350177 (Clockwise)

Preceding gene: 152971573

Following gene: 152971575

Centisome position: 63.0

GC content: 58.25

Gene sequence:

>1425_bases
ATGGCGAGTTTCCCACAGGGATTTTTATGGGGCGGCGCACTGGCCGCCAACCAGTCTGAAGGCGCTTATCTTGAAGGCGG
TAAGGGGCTGACCACCGTGGATACCCTTCCCCACGGCGCCCATCGCCTGCCGGTAAAATTAGGCCTGGAGAAGCGCTTTA
CGCTGCGTGGAGACGAATTTTACCCCAGCCACCAGGCGATCGATTTTTACCATCGCTATAAAGAAGATATCGCCCTGATG
GCCGAGATGGGGTTCAGCGTCTTTCGCACCTCCATCGCCTGGAGCCGCCTGTTCCCACGCGGCGACGAACAGCAGCCGAA
TCCGCAGGGCATCGCCTTCTATCGCTCACTGTTCGAGGAGTGCAAAAAGCACGGTATCGAGCCGCTGGTCACCCTGTGCC
ATTTTGACGTGCCGATGCATCTGGTGATGGAGTACGGCTCCTGGCGCAACCGCAAAATGGTGGATTTTTTCAGCCACTAC
GCCCGCACCTGCTTTGAAGCTTTTGACGGCCTGGTGAAATACTGGCTCACCTTCAATGAAATCAACATCATGCTGCACAG
CCCCTACTCCGGCGCCGGTCTGGTGTTTGAAGAGGGTGAAAACCAGGAGCAGGTGAAGTATCAGGCCGCGCATCACGAGC
TGGTGGCCAGCGCGCTGGCGACGAAAATCGCCCATGAGATTAACCCGCAAAATCAGGTCGGCTGTATGCTGGCCGGCGGT
AATTTCTACCCTTACTCCTGCAAGCCGGAAGATGTATGGATGGCGCTGGAGAAGGACCGCGAAAACCTGTTCTTTATCGA
TGTCCAGGCCCGCGGCGCCTATCCGGTGTGGGCCGCGCGGGTGTTCCGGGAAAAAGGGGTCACGATTGCGAAACAGCCGG
GCGATGACGAGATCCTCAAAAACACCGTCGATTTCGTCTCCTTTAGCTACTACGCGTCGCGCTGCGCCTCGGCGGAGATG
AACGCCAGCAACACCAATGCCGCCAACATCGTCAAGTCGCTGAAGAACCCGCACATTCCGGCCAGCGAATGGGGCTGGGG
CATCGACCCGCTGGGCCTGCGCATCACCATGAACATGATGTACGACCGCTATCAGAAACCGCTGTTCCTGGTGGAAAACG
GGCTTGGCGCCCGCGATGAGATCGATGCCAATGGGGACATCAATGACGACTATCGCATCAGCTACCTGCGCGAGCACATC
AGGGCGATGCGCGATGCTATCGGCGACGGCATTCCGGTGATGGGCTACACCAGCTGGGGCTGCATCGATCTGGTCTCGGC
GTCCACCGGCGAGATGAGCAAGCGCTACGGCTTTGTCTATGTCGATCGCGACGATGCCGGCAACGGCACCCTGGCGCGTA
AGCGTAAAAAGTCATTCTTCTGGTACCAGAAGGTGATAGCCAGTAACGGCGCCGATCTCGACTAA

Upstream 100 bases:

>100_bases
AGGACATTCCCGTTGAGCAGGCCGCTGCCGACGCCAGAGCGCGCCAGGCCAGGGCCCAGGCATCCCACGCCTGAATACAC
TATTGATAATGAGGTAATGC

Downstream 100 bases:

>100_bases
GCACCCTCGCCCACGCGCGCCCGTTCCCCGCGATAACCGGGGGACGGACGTCGGCTATCTGTTGCATCCATTACATGCTT
TTTTCTCGAGCCTGATTCCA

Product: cryptic 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MASFPQGFLWGGALAANQSEGAYLEGGKGLTTVDTLPHGAHRLPVKLGLEKRFTLRGDEFYPSHQAIDFYHRYKEDIALM
AEMGFSVFRTSIAWSRLFPRGDEQQPNPQGIAFYRSLFEECKKHGIEPLVTLCHFDVPMHLVMEYGSWRNRKMVDFFSHY
ARTCFEAFDGLVKYWLTFNEINIMLHSPYSGAGLVFEEGENQEQVKYQAAHHELVASALATKIAHEINPQNQVGCMLAGG
NFYPYSCKPEDVWMALEKDRENLFFIDVQARGAYPVWAARVFREKGVTIAKQPGDDEILKNTVDFVSFSYYASRCASAEM
NASNTNAANIVKSLKNPHIPASEWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGARDEIDANGDINDDYRISYLREHI
RAMRDAIGDGIPVMGYTSWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLARKRKKSFFWYQKVIASNGADLD

Sequences:

>Translated_474_residues
MASFPQGFLWGGALAANQSEGAYLEGGKGLTTVDTLPHGAHRLPVKLGLEKRFTLRGDEFYPSHQAIDFYHRYKEDIALM
AEMGFSVFRTSIAWSRLFPRGDEQQPNPQGIAFYRSLFEECKKHGIEPLVTLCHFDVPMHLVMEYGSWRNRKMVDFFSHY
ARTCFEAFDGLVKYWLTFNEINIMLHSPYSGAGLVFEEGENQEQVKYQAAHHELVASALATKIAHEINPQNQVGCMLAGG
NFYPYSCKPEDVWMALEKDRENLFFIDVQARGAYPVWAARVFREKGVTIAKQPGDDEILKNTVDFVSFSYYASRCASAEM
NASNTNAANIVKSLKNPHIPASEWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGARDEIDANGDINDDYRISYLREHI
RAMRDAIGDGIPVMGYTSWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLARKRKKSFFWYQKVIASNGADLD
>Mature_473_residues
ASFPQGFLWGGALAANQSEGAYLEGGKGLTTVDTLPHGAHRLPVKLGLEKRFTLRGDEFYPSHQAIDFYHRYKEDIALMA
EMGFSVFRTSIAWSRLFPRGDEQQPNPQGIAFYRSLFEECKKHGIEPLVTLCHFDVPMHLVMEYGSWRNRKMVDFFSHYA
RTCFEAFDGLVKYWLTFNEINIMLHSPYSGAGLVFEEGENQEQVKYQAAHHELVASALATKIAHEINPQNQVGCMLAGGN
FYPYSCKPEDVWMALEKDRENLFFIDVQARGAYPVWAARVFREKGVTIAKQPGDDEILKNTVDFVSFSYYASRCASAEMN
ASNTNAANIVKSLKNPHIPASEWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGARDEIDANGDINDDYRISYLREHIR
AMRDAIGDGIPVMGYTSWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLARKRKKSFFWYQKVIASNGADLD

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=500, Percent_Identity=30.8, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI32481206, Length=498, Percent_Identity=28.9156626506024, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI13273313, Length=491, Percent_Identity=26.6802443991853, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI24497614, Length=489, Percent_Identity=27.8118609406953, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28376633, Length=490, Percent_Identity=25.5102040816327, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=86.4978902953586, Blast_Score=881, Evalue=0.0,
Organism=Escherichia coli, GI2367270, Length=473, Percent_Identity=54.3340380549683, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI2367174, Length=478, Percent_Identity=51.4644351464435, Blast_Score=505, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17552856, Length=489, Percent_Identity=29.8568507157464, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17539390, Length=495, Percent_Identity=29.4949494949495, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI21356577, Length=471, Percent_Identity=25.4777070063694, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53660; Mature: 53529

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFPQGFLWGGALAANQSEGAYLEGGKGLTTVDTLPHGAHRLPVKLGLEKRFTLRGDEF
CCCCCCCCEECCEEECCCCCCCEEECCCCCEECCCCCCCCCCCCEEECCCEEEEECCCCC
YPSHQAIDFYHRYKEDIALMAEMGFSVFRTSIAWSRLFPRGDEQQPNPQGIAFYRSLFEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKHGIEPLVTLCHFDVPMHLVMEYGSWRNRKMVDFFSHYARTCFEAFDGLVKYWLTFNE
HHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
INIMLHSPYSGAGLVFEEGENQEQVKYQAAHHELVASALATKIAHEINPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
NFYPYSCKPEDVWMALEKDRENLFFIDVQARGAYPVWAARVFREKGVTIAKQPGDDEILK
CCCCCCCCHHHHHEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCHHHHH
NTVDFVSFSYYASRCASAEMNASNTNAANIVKSLKNPHIPASEWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGARDEIDANGDINDDYRISYLREHIRAMRDAIGDGIPVMGYTSWG
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
CIDLVSASTGEMSKRYGFVYVDRDDAGNGTLARKRKKSFFWYQKVIASNGADLD
HHEEECCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ASFPQGFLWGGALAANQSEGAYLEGGKGLTTVDTLPHGAHRLPVKLGLEKRFTLRGDEF
CCCCCCCEECCEEECCCCCCCEEECCCCCEECCCCCCCCCCCCEEECCCEEEEECCCCC
YPSHQAIDFYHRYKEDIALMAEMGFSVFRTSIAWSRLFPRGDEQQPNPQGIAFYRSLFEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKHGIEPLVTLCHFDVPMHLVMEYGSWRNRKMVDFFSHYARTCFEAFDGLVKYWLTFNE
HHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
INIMLHSPYSGAGLVFEEGENQEQVKYQAAHHELVASALATKIAHEINPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
NFYPYSCKPEDVWMALEKDRENLFFIDVQARGAYPVWAARVFREKGVTIAKQPGDDEILK
CCCCCCCCHHHHHEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCHHHHH
NTVDFVSFSYYASRCASAEMNASNTNAANIVKSLKNPHIPASEWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGARDEIDANGDINDDYRISYLREHIRAMRDAIGDGIPVMGYTSWG
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
CIDLVSASTGEMSKRYGFVYVDRDDAGNGTLARKRKKSFFWYQKVIASNGADLD
HHEEECCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]