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The map label for this gene is ascF [H]

Identifier: 152971573

GI number: 152971573

Start: 3347284

End: 3348726

Strand: Direct

Name: ascF [H]

Synonym: KPN_03051

Alternate gene names: 152971573

Gene position: 3347284-3348726 (Clockwise)

Preceding gene: 152971568

Following gene: 152971574

Centisome position: 62.98

GC content: 62.51

Gene sequence:

>1443_bases
ATGTCGAAAAATTATGCAGCCCTGGCGCAACAGATAGTCGCGGCAATCGGCGGGGTCGACAACGTCGCCGCCGTTACCCA
CTGCATGACCCGTCTGCGCTTCGTGGTTAAAGACGACGAACAGGTCGATAGCTCTACCCTGAAAGGCCTGGCCGGGGTGC
TCGGCGTCGTGCGCAGCGATAACCAGTGCCAGGTGATCATCGGCAACACTGTCTCCCAGGCCTACCGCGAGGTGGTGAAT
CTGCTGCCTGGCGACCTGCGCCCGGCTGAGCCGCAGGGCAAAGCGCCGCTAACCCTGAAACGCATCGGCGCCGGGATCCT
TGACGCGCTGATCGGCACCATGTCGCCGCTGATCCCGGCGATTATCGGCGGATCGATGGTCAAGCTGCTGGCGATGATCC
TCGAGATGAGCGGCGCGTTGCCGAAAGGCTCGCCGACGCTGACCCTGCTGGCGCTTATCGGCGACGGCGCATTTTTCTTC
CTGCCGCTGATGGTGGCCGCCTCCGCGGCGGTGAAATTCAAAACTAATATGTCGCTGGCTATCGCCATCGCCGGGGTGCT
GGTGCATCCTGGGTTTATCGAATTGATGGCCAAAGCCGCCCAGGGAGAACACGTTGAATTCGCCTTTATTCCGGTTACCG
CGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGTGCCTGTCGTATATCGAGCGCTGGGTGGATCGC
ATTACCCCGGCGGTGACGAAAAACTTTCTGAAGCCGATGCTGATCGTGTTGATCGCCGCCCCGCTGGCGATCCTGCTGAT
TGGCCCGCTGGGCATCTGGATCGGCAGCGCCATTTCCGCGCTGGTGTATACGATCCACAGCTATCTCGGCTGGCTGTCGG
TGGCGATCATGGGCGGGCTGTGGCCGCTGTTGGTGATGACCGGTATGCACCGCGTCTTTACGCCGACCATCATTCAGACC
ATCGCCGAAACCGGCAAAGAGGGTATGGTGATGCCATCGGAGATCGGCGCCAACCTGTCGCTCGGCGGCTCTTCGCTGGC
GGTAGCGTGGAAAACCAAAAACCCGGAGCTGCGCCAGACGGCGCTGGCCGCCGCGGCATCGGCGATTCTGGCCGGGATTT
CCGAACCGGCGCTGTACGGCGTGGCGGTGCGCCTGAAGCGCCCGCTGATCGCCAGCTTAATCAGCGGCTTTATCTGCGGC
GCGGTGGCAGGCATTGCCGGACTGGCCAGCCATTCGATGGCGGCGCCGGGGCTGTTTACCAGCGTGCAGTTTTTCGATCC
GGCCAATCCAATGACCATCGTCTGGGTGTTTGGCGTGATGGCGCTGTCGGTGGTGCTGTCGTTTGCCCTGACGCTGATCC
TTGGCTTTGAGGACATTCCCGTTGAGCAGGCCGCTGCCGACGCCAGAGCGCGCCAGGCCAGGGCCCAGGCATCCCACGCC
TGA

Upstream 100 bases:

>100_bases
CATGATTTTGTGGAACCGGTTTCCTATAAAAACATGGTATCGCCCGGCGATGCGGGGCGGTGGCAGATTAGTACCCTATT
GATAAACAGGATAAAATCCG

Downstream 100 bases:

>100_bases
ATACACTATTGATAATGAGGTAATGCATGGCGAGTTTCCCACAGGGATTTTTATGGGGCGGCGCACTGGCCGCCAACCAG
TCTGAAGGCGCTTATCTTGA

Product: PTS system cellobiose/arbutin/salicin-specific transporter subunits IIBC

Products: NA

Alternate protein names: EIIBC-Asc; EII-Asc; Arbutin-, cellobiose-, and salicin-specific phosphotransferase enzyme IIB component; PTS system arbutin-, cellobiose-, and salicin-specific EIIB component; Arbutin, cellobiose, and salicin permease IIC component; PTS system arbutin-, cellobiose-, and salicin-specific EIIC component [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSKNYAALAQQIVAAIGGVDNVAAVTHCMTRLRFVVKDDEQVDSSTLKGLAGVLGVVRSDNQCQVIIGNTVSQAYREVVN
LLPGDLRPAEPQGKAPLTLKRIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGALPKGSPTLTLLALIGDGAFFF
LPLMVAASAAVKFKTNMSLAIAIAGVLVHPGFIELMAKAAQGEHVEFAFIPVTAVKYTYTVIPALVMTWCLSYIERWVDR
ITPAVTKNFLKPMLIVLIAAPLAILLIGPLGIWIGSAISALVYTIHSYLGWLSVAIMGGLWPLLVMTGMHRVFTPTIIQT
IAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASAILAGISEPALYGVAVRLKRPLIASLISGFICG
AVAGIAGLASHSMAAPGLFTSVQFFDPANPMTIVWVFGVMALSVVLSFALTLILGFEDIPVEQAAADARARQARAQASHA

Sequences:

>Translated_480_residues
MSKNYAALAQQIVAAIGGVDNVAAVTHCMTRLRFVVKDDEQVDSSTLKGLAGVLGVVRSDNQCQVIIGNTVSQAYREVVN
LLPGDLRPAEPQGKAPLTLKRIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGALPKGSPTLTLLALIGDGAFFF
LPLMVAASAAVKFKTNMSLAIAIAGVLVHPGFIELMAKAAQGEHVEFAFIPVTAVKYTYTVIPALVMTWCLSYIERWVDR
ITPAVTKNFLKPMLIVLIAAPLAILLIGPLGIWIGSAISALVYTIHSYLGWLSVAIMGGLWPLLVMTGMHRVFTPTIIQT
IAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASAILAGISEPALYGVAVRLKRPLIASLISGFICG
AVAGIAGLASHSMAAPGLFTSVQFFDPANPMTIVWVFGVMALSVVLSFALTLILGFEDIPVEQAAADARARQARAQASHA
>Mature_479_residues
SKNYAALAQQIVAAIGGVDNVAAVTHCMTRLRFVVKDDEQVDSSTLKGLAGVLGVVRSDNQCQVIIGNTVSQAYREVVNL
LPGDLRPAEPQGKAPLTLKRIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGALPKGSPTLTLLALIGDGAFFFL
PLMVAASAAVKFKTNMSLAIAIAGVLVHPGFIELMAKAAQGEHVEFAFIPVTAVKYTYTVIPALVMTWCLSYIERWVDRI
TPAVTKNFLKPMLIVLIAAPLAILLIGPLGIWIGSAISALVYTIHSYLGWLSVAIMGGLWPLLVMTGMHRVFTPTIIQTI
AETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASAILAGISEPALYGVAVRLKRPLIASLISGFICGA
VAGIAGLASHSMAAPGLFTSVQFFDPANPMTIVWVFGVMALSVVLSFALTLILGFEDIPVEQAAADARARQARAQASHA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI48994906, Length=473, Percent_Identity=89.2177589852008, Blast_Score=838, Evalue=0.0,
Organism=Escherichia coli, GI1790159, Length=460, Percent_Identity=31.0869565217391, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI2367362, Length=432, Percent_Identity=27.7777777777778, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788769, Length=413, Percent_Identity=27.1186440677966, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 50315; Mature: 50184

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNYAALAQQIVAAIGGVDNVAAVTHCMTRLRFVVKDDEQVDSSTLKGLAGVLGVVRSD
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
NQCQVIIGNTVSQAYREVVNLLPGDLRPAEPQGKAPLTLKRIGAGILDALIGTMSPLIPA
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IIGGSMVKLLAMILEMSGALPKGSPTLTLLALIGDGAFFFLPLMVAASAAVKFKTNMSLA
HHCHHHHHHHHHHHHHHCCCCCCCCHHEEHHHHHCCHHHHHHHHHHHHHHHEEECCCHHH
IAIAGVLVHPGFIELMAKAAQGEHVEFAFIPVTAVKYTYTVIPALVMTWCLSYIERWVDR
HHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITPAVTKNFLKPMLIVLIAAPLAILLIGPLGIWIGSAISALVYTIHSYLGWLSVAIMGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQT
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEEEECCCCHHHHH
ALAAAASAILAGISEPALYGVAVRLKRPLIASLISGFICGAVAGIAGLASHSMAAPGLFT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SVQFFDPANPMTIVWVFGVMALSVVLSFALTLILGFEDIPVEQAAADARARQARAQASHA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKNYAALAQQIVAAIGGVDNVAAVTHCMTRLRFVVKDDEQVDSSTLKGLAGVLGVVRSD
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
NQCQVIIGNTVSQAYREVVNLLPGDLRPAEPQGKAPLTLKRIGAGILDALIGTMSPLIPA
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IIGGSMVKLLAMILEMSGALPKGSPTLTLLALIGDGAFFFLPLMVAASAAVKFKTNMSLA
HHCHHHHHHHHHHHHHHCCCCCCCCHHEEHHHHHCCHHHHHHHHHHHHHHHEEECCCHHH
IAIAGVLVHPGFIELMAKAAQGEHVEFAFIPVTAVKYTYTVIPALVMTWCLSYIERWVDR
HHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITPAVTKNFLKPMLIVLIAAPLAILLIGPLGIWIGSAISALVYTIHSYLGWLSVAIMGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQT
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEEEECCCCHHHHH
ALAAAASAILAGISEPALYGVAVRLKRPLIASLISGFICGAVAGIAGLASHSMAAPGLFT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SVQFFDPANPMTIVWVFGVMALSVVLSFALTLILGFEDIPVEQAAADARARQARAQASHA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]