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The map label for this gene is hisH [H]

Identifier: 152971018

GI number: 152971018

Start: 2704104

End: 2704694

Strand: Direct

Name: hisH [H]

Synonym: KPN_02479

Alternate gene names: 152971018

Gene position: 2704104-2704694 (Clockwise)

Preceding gene: 152971017

Following gene: 152971019

Centisome position: 50.88

GC content: 60.58

Gene sequence:

>591_bases
ATGAATGTGGTGATCCTTGATACCGGCTGCGCCAACCTCAATTCGGTGAAATCGGCGATTGGCCGCCACGGCTATGAGCC
AGTGGTCAGCCGCGATCCGGAAGTGGTATTGCGTGCCGATAAACTGTTTCTTCCCGGCGTCGGCACCGCCCAGGCGGCGA
TGGATCAGCTGCGCGACCGGGATCTGATCGACCTGATCAAAGCCTGTACCCAGCCGGTGCTGGGGATTTGCCTGGGCATG
CAGCTGCTCGGCAAGCGCAGCGAGGAGAACAACGGCGTCGATCTGCTGGGCATTATCGAAGAAGAGGTGCCGAAGATGAC
CGACCACGGTCTGCCGCTGCCGCACATGGGCTGGAACCGGGTGTATGCCAAAGCTGGCGATCGGCTGTTCCGCGGCATTG
AAGAAGGCGCGTATTTCTACTTCGTGCACAGCTACGCCATGCCGGTCAACCCGTACACCATCGCCCAGTGCAATTACGGC
GAGGCGTTCACCGCCGCGGTGCAGAAAGATAACTTCTTTGGCGTGCAGTTTCACCCGGAGCGCTCCGGGAGCGCCGGGGC
GCAGCTGCTGAAAAACTTCCTGGAGATGTGA

Upstream 100 bases:

>100_bases
ACCACCGCGTGGAGAGCCTGTTCAAAGCCTTTGGCCGTACGCTGCGCCAGGCGATCCGCGTCCAGGGCGATGCCCTGCCG
TCTTCCAAAGGGGTGCTGTA

Downstream 100 bases:

>100_bases
TGATTATTCCTGCTTTAGATTTAATCGACGGCACCGTCGTTCGTCTCCATCAGGGCGATTACGGCCAGCAGCGCGACTAT
GGCAGCGATCCGCTGCCGCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNVVILDTGCANLNSVKSAIGRHGYEPVVSRDPEVVLRADKLFLPGVGTAQAAMDQLRDRDLIDLIKACTQPVLGICLGM
QLLGKRSEENNGVDLLGIIEEEVPKMTDHGLPLPHMGWNRVYAKAGDRLFRGIEEGAYFYFVHSYAMPVNPYTIAQCNYG
EAFTAAVQKDNFFGVQFHPERSGSAGAQLLKNFLEM

Sequences:

>Translated_196_residues
MNVVILDTGCANLNSVKSAIGRHGYEPVVSRDPEVVLRADKLFLPGVGTAQAAMDQLRDRDLIDLIKACTQPVLGICLGM
QLLGKRSEENNGVDLLGIIEEEVPKMTDHGLPLPHMGWNRVYAKAGDRLFRGIEEGAYFYFVHSYAMPVNPYTIAQCNYG
EAFTAAVQKDNFFGVQFHPERSGSAGAQLLKNFLEM
>Mature_196_residues
MNVVILDTGCANLNSVKSAIGRHGYEPVVSRDPEVVLRADKLFLPGVGTAQAAMDQLRDRDLIDLIKACTQPVLGICLGM
QLLGKRSEENNGVDLLGIIEEEVPKMTDHGLPLPHMGWNRVYAKAGDRLFRGIEEGAYFYFVHSYAMPVNPYTIAQCNYG
EAFTAAVQKDNFFGVQFHPERSGSAGAQLLKNFLEM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=88.265306122449, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=34.4497607655502, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21604; Mature: 21604

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00442 GATASE_TYPE_I ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVILDTGCANLNSVKSAIGRHGYEPVVSRDPEVVLRADKLFLPGVGTAQAAMDQLRDR
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECEEECCCCCHHHHHHHHHCCC
DLIDLIKACTQPVLGICLGMQLLGKRSEENNGVDLLGIIEEEVPKMTDHGLPLPHMGWNR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCHHH
VYAKAGDRLFRGIEEGAYFYFVHSYAMPVNPYTIAQCNYGEAFTAAVQKDNFFGVQFHPE
HHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEECCCCCHHHHHCCCCCEEEEEECCC
RSGSAGAQLLKNFLEM
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNVVILDTGCANLNSVKSAIGRHGYEPVVSRDPEVVLRADKLFLPGVGTAQAAMDQLRDR
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECEEECCCCCHHHHHHHHHCCC
DLIDLIKACTQPVLGICLGMQLLGKRSEENNGVDLLGIIEEEVPKMTDHGLPLPHMGWNR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCHHH
VYAKAGDRLFRGIEEGAYFYFVHSYAMPVNPYTIAQCNYGEAFTAAVQKDNFFGVQFHPE
HHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEECCCCCHHHHHCCCCCEEEEEECCC
RSGSAGAQLLKNFLEM
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10222209; 11206551; 11258796 [H]