Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 152970997

GI number: 152970997

Start: 2680059

End: 2681378

Strand: Direct

Name: shiA [H]

Synonym: KPN_02458

Alternate gene names: 152970997

Gene position: 2680059-2681378 (Clockwise)

Preceding gene: 152970991

Following gene: 152970998

Centisome position: 50.42

GC content: 61.52

Gene sequence:

>1320_bases
ATGGATTCAACAATAAGCGTGCAGCCCGGCGAGGCGCCAGATTCACTACATCGGGCCCGGCGGGCGGCCTGGGGTAGTTT
TGCCGGCGCGGTGGTCGACTGGTACGATTTTCTTCTCTATGGCATTACCGCCGCGCTGGTGTTCAACCGCGAATTTTTCC
CGCAAATCGGCCCGGCGATGGGAACGCTTGCCGCCTTTGCCACCTTCGGCGTTGGGTTTCTTTTTCGCCCGCTCGGCGGA
ATTATTTTTGGCCACTTCGGCGATCGCCTCGGGCGGAAACGGATGCTGATGATGACCGTCTGGATGATGGGTATCGCCAC
TGCCTGCATCGGCCTGCTCCCCTCGTTCAACCAGATCGGCTGGTGGGCGCCGGTGCTGCTGGTGTTCCTGCGAGCGGTTC
AGGGATTCGCCGTCGGCGGCGAATGGGGCGGCGCGGCGCTGCTCTCGGTGGAGAATGCCCCACAGGGGAAAAAAGCCTTT
TACAGCAGCGGGGTGCAGGTGGGCTACGGCGTCGGCCTCCTGCTCTCTACCGGGCTGGTATCGCTGATTAGCAGCCTGAC
CAGCGACCAGCAGTTCCTCAGCTGGGGCTGGCGCGTACCGTTCCTGTTCAGCGTGGTGCTGGTCCTTATTGCGCTGTGGA
TCCGTAACGGCATGGCGGAATCGCAGGAGTTTGAAGCGCAGCAGAGCCAGGACAACGCGCCGCAGATGAAAAAGCGGCTA
CCGGTAGTGGAAGCGCTGCTTCGCCATCCGGGAGCGTTTTTACTGATTATCGCCCTGCGGCTGTGTGAACTGCTGACGAT
GTATATCGTCACCGCCTTCGCCCTCAACTACTCCACGCAAAACCTCGGCCTGCCGCGCGAGCTGTTTCTCAACATTGGCC
TGCTGGTGGGCGGTCTTAGCTGCCTGACTATCCCCTGTTTCGCCTGGCTGGCAGACCGCTTTGGCCGTCGGCGGATCTAT
ATCACCGGGGCGCTGATCGGCACTTTGAGCGGTTTCCCGTTCTTTATGGCGCTGGAGAGTCAGTCCGTTTTCTGGATCCT
GTTCTTCGCCCTGATGCTGGCCAATATCGCCCATGATATGGTGGTCTGCGTCCAGCAGCCGATGTTTACCGAGCTGTTCG
GCGCCAGCTATCGCTATAGCGGCGCCGGCGTCGGCTACCAGGTGGCGAGCGTGGTCGGCGGCGGCTTTACCCCGTTTATT
GCCGCGGCGCTGGTCACCTTCTCCGGCGGATCGTGGCACAGCGTGGCCCTCTATCTGACCGCAGGATGCCTGCTGTCAGC
GCTCACCGCGCTGATGATGAAAAAGCACCCCGTCGACTGA

Upstream 100 bases:

>100_bases
ACTTTCCTCCACGGCCGCTGCGCGGTAGTCTCAGAGCAGACGCAACGGTAAAAGCGTCTCATTCCAATAACAATGAAGTA
CGTGACTGACAGGACAGGGT

Downstream 100 bases:

>100_bases
CCCACCAGCGGAGGGCGTAACCCGCGCCCTCCGGTAAGGAGAATGCATGTCTCAGTTAACCCTGGCCGACTGCTGGCCTC
GCCGTTTTTCCCCCTCTTCG

Product: shikimate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MDSTISVQPGEAPDSLHRARRAAWGSFAGAVVDWYDFLLYGITAALVFNREFFPQIGPAMGTLAAFATFGVGFLFRPLGG
IIFGHFGDRLGRKRMLMMTVWMMGIATACIGLLPSFNQIGWWAPVLLVFLRAVQGFAVGGEWGGAALLSVENAPQGKKAF
YSSGVQVGYGVGLLLSTGLVSLISSLTSDQQFLSWGWRVPFLFSVVLVLIALWIRNGMAESQEFEAQQSQDNAPQMKKRL
PVVEALLRHPGAFLLIIALRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRIY
ITGALIGTLSGFPFFMALESQSVFWILFFALMLANIAHDMVVCVQQPMFTELFGASYRYSGAGVGYQVASVVGGGFTPFI
AAALVTFSGGSWHSVALYLTAGCLLSALTALMMKKHPVD

Sequences:

>Translated_439_residues
MDSTISVQPGEAPDSLHRARRAAWGSFAGAVVDWYDFLLYGITAALVFNREFFPQIGPAMGTLAAFATFGVGFLFRPLGG
IIFGHFGDRLGRKRMLMMTVWMMGIATACIGLLPSFNQIGWWAPVLLVFLRAVQGFAVGGEWGGAALLSVENAPQGKKAF
YSSGVQVGYGVGLLLSTGLVSLISSLTSDQQFLSWGWRVPFLFSVVLVLIALWIRNGMAESQEFEAQQSQDNAPQMKKRL
PVVEALLRHPGAFLLIIALRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRIY
ITGALIGTLSGFPFFMALESQSVFWILFFALMLANIAHDMVVCVQQPMFTELFGASYRYSGAGVGYQVASVVGGGFTPFI
AAALVTFSGGSWHSVALYLTAGCLLSALTALMMKKHPVD
>Mature_439_residues
MDSTISVQPGEAPDSLHRARRAAWGSFAGAVVDWYDFLLYGITAALVFNREFFPQIGPAMGTLAAFATFGVGFLFRPLGG
IIFGHFGDRLGRKRMLMMTVWMMGIATACIGLLPSFNQIGWWAPVLLVFLRAVQGFAVGGEWGGAALLSVENAPQGKKAF
YSSGVQVGYGVGLLLSTGLVSLISSLTSDQQFLSWGWRVPFLFSVVLVLIALWIRNGMAESQEFEAQQSQDNAPQMKKRL
PVVEALLRHPGAFLLIIALRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRIY
ITGALIGTLSGFPFFMALESQSVFWILFFALMLANIAHDMVVCVQQPMFTELFGASYRYSGAGVGYQVASVVGGGFTPFI
AAALVTFSGGSWHSVALYLTAGCLLSALTALMMKKHPVD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=436, Percent_Identity=85.0917431192661, Blast_Score=755, Evalue=0.0,
Organism=Escherichia coli, GI1789941, Length=432, Percent_Identity=38.8888888888889, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1790550, Length=425, Percent_Identity=32, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788942, Length=435, Percent_Identity=28.2758620689655, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87082404, Length=401, Percent_Identity=23.1920199501247, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082231, Length=214, Percent_Identity=24.2990654205607, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47718; Mature: 47718

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00626 RCC1_2 ; PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]