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The map label for this gene is gcvA [H]

Identifier: 152970991

GI number: 152970991

Start: 2675517

End: 2676431

Strand: Direct

Name: gcvA [H]

Synonym: KPN_02452

Alternate gene names: 152970991

Gene position: 2675517-2676431 (Clockwise)

Preceding gene: 152970988

Following gene: 152970997

Centisome position: 50.34

GC content: 64.81

Gene sequence:

>915_bases
ATGAGACCGCCCCGTCTGCCGCCGCTGGGGGCGCTACGCGCTTTTCATGCCGTCGCCCGCCACCGCAGTTTTAAACAGGC
CGCCGAGGCGCTCGGCGTCAGCGCGACGGCGGTCAGCCATCAGATTAAACTGCTGGAGTCGGTGCTGGAGTGCCGGGTCT
GCGAACGCAGCGCGCAGGGCGTCAGTCTGAACGCTGATGGCAAGATCCTTTACGCGGCCACCCAACGCGCCTTTAGCGCC
CTGGAACAGGCCGTCGTCCAGATCGCTCATGCCCGTCAGCCGCCATCCTTAACCGTCACCACCACCTCCAATTTTCTCAC
CCACTGGCTGGTGCCGCGGCTGGCGGATTTCACCGCCCGCTTTCCGGCTATCGACCTGCGGCTACACACCAGCGTCGAAC
GAGTGGATCTGCATCTGGGCACGGTAGACGCCGCGATTCGCTATCGTGAAACGCCGGAGCCGGACCTGTACTGCACGCTG
CTGTATGAGGATCGCTTTATCGTGGTCGCCAGCCCGACTCTGGGCCTCAGCCGGCCTGAAGATCTGCAGCGGGTGACGCT
GTTTCACGTTGCCAACCGGCGCGTGCCAGCTGATTCGCCGAGCTGGGAGAACTGGCGTCGGCGCTATGGACCGCCGACGC
TGAATATTGATGCGGGGTTAACCTTTAGCGATGAGACCCATGCCCTGCAGGCGGCGGTGGCCGGTCAGGGGGTGGTGATC
GCCAGCGAACTGCTGGCGCGGGATCTGCTGCAGCGCGGCGTATTGTCCGCGCCGTTCAGCAACGCATTGCCTGGCGCCCG
CTACTACCTGGTCACTACCGAAGCGGTAGCGCAGCGGGCGGATATTATTGCTCTGCGGGAGTGGCTGCTCAGCCAGATGG
CGTCGGGCGACGGCGGCCATCCGACTGCGGGATAG

Upstream 100 bases:

>100_bases
TTTAATTCATGTCATTGTCTTTACATGCCCGTAGCCTATCGCCACAATATCGCCTTACACAAACGCATAGTTTTCAGCCA
TAGATGAGAAAAACTCAGCC

Downstream 100 bases:

>100_bases
CGGGCTTACCAGCCGCAGACGTTGGTCTCTTCATCGGACTCGTAGCCGCGAACGATATTGATCAGATCCTTCACCGTATC
AGCGGCGGTGAGCGGGTCGC

Product: transcriptional regulator LysR

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRPPRLPPLGALRAFHAVARHRSFKQAAEALGVSATAVSHQIKLLESVLECRVCERSAQGVSLNADGKILYAATQRAFSA
LEQAVVQIAHARQPPSLTVTTTSNFLTHWLVPRLADFTARFPAIDLRLHTSVERVDLHLGTVDAAIRYRETPEPDLYCTL
LYEDRFIVVASPTLGLSRPEDLQRVTLFHVANRRVPADSPSWENWRRRYGPPTLNIDAGLTFSDETHALQAAVAGQGVVI
ASELLARDLLQRGVLSAPFSNALPGARYYLVTTEAVAQRADIIALREWLLSQMASGDGGHPTAG

Sequences:

>Translated_304_residues
MRPPRLPPLGALRAFHAVARHRSFKQAAEALGVSATAVSHQIKLLESVLECRVCERSAQGVSLNADGKILYAATQRAFSA
LEQAVVQIAHARQPPSLTVTTTSNFLTHWLVPRLADFTARFPAIDLRLHTSVERVDLHLGTVDAAIRYRETPEPDLYCTL
LYEDRFIVVASPTLGLSRPEDLQRVTLFHVANRRVPADSPSWENWRRRYGPPTLNIDAGLTFSDETHALQAAVAGQGVVI
ASELLARDLLQRGVLSAPFSNALPGARYYLVTTEAVAQRADIIALREWLLSQMASGDGGHPTAG
>Mature_304_residues
MRPPRLPPLGALRAFHAVARHRSFKQAAEALGVSATAVSHQIKLLESVLECRVCERSAQGVSLNADGKILYAATQRAFSA
LEQAVVQIAHARQPPSLTVTTTSNFLTHWLVPRLADFTARFPAIDLRLHTSVERVDLHLGTVDAAIRYRETPEPDLYCTL
LYEDRFIVVASPTLGLSRPEDLQRVTLFHVANRRVPADSPSWENWRRRYGPPTLNIDAGLTFSDETHALQAAVAGQGVVI
ASELLARDLLQRGVLSAPFSNALPGARYYLVTTEAVAQRADIIALREWLLSQMASGDGGHPTAG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=37.037037037037, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1786448, Length=297, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=30.0675675675676, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87081978, Length=244, Percent_Identity=27.8688524590164, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787128, Length=144, Percent_Identity=32.6388888888889, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=305, Percent_Identity=25.5737704918033, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI157672245, Length=187, Percent_Identity=33.1550802139037, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33294; Mature: 33294

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPRLPPLGALRAFHAVARHRSFKQAAEALGVSATAVSHQIKLLESVLECRVCERSAQG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VSLNADGKILYAATQRAFSALEQAVVQIAHARQPPSLTVTTTSNFLTHWLVPRLADFTAR
EEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHC
FPAIDLRLHTSVERVDLHLGTVDAAIRYRETPEPDLYCTLLYEDRFIVVASPTLGLSRPE
CCEEEEEEECCCEEHEEEECCHHHHHEECCCCCCCEEEEEEEECEEEEEECCCCCCCCCH
DLQRVTLFHVANRRVPADSPSWENWRRRYGPPTLNIDAGLTFSDETHALQAAVAGQGVVI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEECCCCEECCCHHHHHHHHCCCCHHH
ASELLARDLLQRGVLSAPFSNALPGARYYLVTTEAVAQRADIIALREWLLSQMASGDGGH
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PTAG
CCCC
>Mature Secondary Structure
MRPPRLPPLGALRAFHAVARHRSFKQAAEALGVSATAVSHQIKLLESVLECRVCERSAQG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VSLNADGKILYAATQRAFSALEQAVVQIAHARQPPSLTVTTTSNFLTHWLVPRLADFTAR
EEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHC
FPAIDLRLHTSVERVDLHLGTVDAAIRYRETPEPDLYCTLLYEDRFIVVASPTLGLSRPE
CCEEEEEEECCCEEHEEEECCHHHHHEECCCCCCCEEEEEEEECEEEEEECCCCCCCCCH
DLQRVTLFHVANRRVPADSPSWENWRRRYGPPTLNIDAGLTFSDETHALQAAVAGQGVVI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEECCCCEECCCHHHHHHHHCCCCHHH
ASELLARDLLQRGVLSAPFSNALPGARYYLVTTEAVAQRADIIALREWLLSQMASGDGGH
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PTAG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]