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The map label for this gene is appA [H]

Identifier: 152970568

GI number: 152970568

Start: 2211679

End: 2213322

Strand: Direct

Name: appA [H]

Synonym: KPN_02019

Alternate gene names: 152970568

Gene position: 2211679-2213322 (Clockwise)

Preceding gene: 152970567

Following gene: 152970569

Centisome position: 41.61

GC content: 59.25

Gene sequence:

>1644_bases
GTGGCCCTGCTCGGCGGCGTGGTAGCATTTTCTTCTGTTGCCCATGCGGAAGTGATCCTGAAAGAAGGTATCACCCCGGC
CACTGACGCCAGCCAGATCCCGGCCAGCGCCAAACTGCGGAAAGACACGGTGGTGGCCGGGATCTCCGAGCCGCAGGGGA
TCTTTAACCCCTACTTTTTCGTCAACGGCTGGGATGAGAACGTGACTAACGTCATTTTTTCACGCCTGATCGACTGGGAC
AGCCAGGGCAAGCTGGTCCCGGGACTGGCGGAGAGCTGGACGGTCAGCCCGGACAGTAAAGTCTATACCATCAAATTGCG
CCCTGGTCTCACCTTTAGCGACGGCTCACCGCTGACGGCGGAAGATGTCGCCTTTACCCTGACGGTCCTGCTCGACCCCA
GCTATGATGGCGATACCGACATCACCCTGGCCAATATCGCTGGCGGGGCGGACTACAAAGCCGGCAAGGCTGACAGCGTC
AGCGGCCTGAAGGTCATCGACCCGCTCACTCTGCAGGTGACCACCACGCAGCCAGGCGCCACCACGCTGGCCAAAATTGG
CGGGCCTGTACTGTCGAAAGCCTGGTACGGCAAAGGGTATCAGCGGGGAAATCTCGACTATCTGCGCTCACTGCACGGCA
AGCCGCTGGGCAACGGCCCTTACGTGTACGACAAATATATTCCCGGTCAGGAAATTCGCTTCCATGCCAATAGCCATTTT
TACCGTGGGACGCCGCCAACGCCGCGCTTTATCTATCGCGTGACCAATCCGTCCACCAACTTCCAGCTGTTCCAGACCGG
GGAGACCGACTACGACGCCTTTACCTCGCGCCCCGACGATATTGAGCAACTGAAGATGCTCGGCTTCGCCAATATTAATC
TTTACGGCTCCAGCGACTACAGCCAGGTGGAATTCAACGTGCATCGCCCGGCGCTACAGGATAAGCGGGTGCGCCAGGCG
CTGATTTACGGCCTGGATAGGCAGAAGCTGATCGACGTGGTCTATCAGGGATACGGCAAAGTCGCCATCGAACCGATCGC
GCCGATCTCCTGGGCCTTTAACGCTGAAGGCGTCAACCCTTACCCTTACGATCCGGCCCAGGCCAAAAAGCTGCTGGATG
AGGCCGGCTGGAAACCAGGCGCCGACGGTATCCGCGCCAAAGACGGCCAGCGCCTGGAGCTGACCCTGCTGGTGAGCAAG
AAGGTGCTCAACGATGCGCTGATCCCCATCGCCAAGGAGAACTGGCGGCAGATCGGCGTGCTGCTGAAGCCGCAGGTGGT
GGATTTCAACGCCCTGATGGCGCAGCGCAAAGCCGGAAATTACGATTTGGCCTCGTTCAGCACCAGCACCCTCAACGACC
CGCACGACGGGGTATGGGATTTCTACAGCAGCGAAGCTAAAGAGTCCGGCTACCATAACGCGGAAGTCGATAAGCTGATT
AACGCCGGCAACGCGGTGCTCGACATCGAACAGCGCAAGCCGATCTACCACCAGCTGTATAAAGTGCTGGCCGACGATCC
GCCGGTGATCCTGCTCGGCTATCGCGAGATCCTTTCCGCCAGCAGCGCCCGCGTCAGCGGCTTTAAGCCGGATATCTACA
ACGGCCTGACCGGCAGCCTGCCGGATGTAAAAATCGTCAAATAA

Upstream 100 bases:

>100_bases
ATTGTAAATAACCACGCAATCATTTGATGAGGTAGCACTTTTGTCCAGATTACAACAAGGATTAAAAACCAGACAAACCC
GCTTCGCCCTGGCGCTAAAA

Downstream 100 bases:

>100_bases
ATCCCTGGCCGCCGGGTTAACCGGCGGCTGATGTGAGACCGTCATGAGAAATTTCATCCTGAGGCGCCTGCTGCAAACGC
TGCCGATGCTGCTGCTGGCC

Product: putative oligopeptide ABC transporter, oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MALLGGVVAFSSVAHAEVILKEGITPATDASQIPASAKLRKDTVVAGISEPQGIFNPYFFVNGWDENVTNVIFSRLIDWD
SQGKLVPGLAESWTVSPDSKVYTIKLRPGLTFSDGSPLTAEDVAFTLTVLLDPSYDGDTDITLANIAGGADYKAGKADSV
SGLKVIDPLTLQVTTTQPGATTLAKIGGPVLSKAWYGKGYQRGNLDYLRSLHGKPLGNGPYVYDKYIPGQEIRFHANSHF
YRGTPPTPRFIYRVTNPSTNFQLFQTGETDYDAFTSRPDDIEQLKMLGFANINLYGSSDYSQVEFNVHRPALQDKRVRQA
LIYGLDRQKLIDVVYQGYGKVAIEPIAPISWAFNAEGVNPYPYDPAQAKKLLDEAGWKPGADGIRAKDGQRLELTLLVSK
KVLNDALIPIAKENWRQIGVLLKPQVVDFNALMAQRKAGNYDLASFSTSTLNDPHDGVWDFYSSEAKESGYHNAEVDKLI
NAGNAVLDIEQRKPIYHQLYKVLADDPPVILLGYREILSASSARVSGFKPDIYNGLTGSLPDVKIVK

Sequences:

>Translated_547_residues
MALLGGVVAFSSVAHAEVILKEGITPATDASQIPASAKLRKDTVVAGISEPQGIFNPYFFVNGWDENVTNVIFSRLIDWD
SQGKLVPGLAESWTVSPDSKVYTIKLRPGLTFSDGSPLTAEDVAFTLTVLLDPSYDGDTDITLANIAGGADYKAGKADSV
SGLKVIDPLTLQVTTTQPGATTLAKIGGPVLSKAWYGKGYQRGNLDYLRSLHGKPLGNGPYVYDKYIPGQEIRFHANSHF
YRGTPPTPRFIYRVTNPSTNFQLFQTGETDYDAFTSRPDDIEQLKMLGFANINLYGSSDYSQVEFNVHRPALQDKRVRQA
LIYGLDRQKLIDVVYQGYGKVAIEPIAPISWAFNAEGVNPYPYDPAQAKKLLDEAGWKPGADGIRAKDGQRLELTLLVSK
KVLNDALIPIAKENWRQIGVLLKPQVVDFNALMAQRKAGNYDLASFSTSTLNDPHDGVWDFYSSEAKESGYHNAEVDKLI
NAGNAVLDIEQRKPIYHQLYKVLADDPPVILLGYREILSASSARVSGFKPDIYNGLTGSLPDVKIVK
>Mature_546_residues
ALLGGVVAFSSVAHAEVILKEGITPATDASQIPASAKLRKDTVVAGISEPQGIFNPYFFVNGWDENVTNVIFSRLIDWDS
QGKLVPGLAESWTVSPDSKVYTIKLRPGLTFSDGSPLTAEDVAFTLTVLLDPSYDGDTDITLANIAGGADYKAGKADSVS
GLKVIDPLTLQVTTTQPGATTLAKIGGPVLSKAWYGKGYQRGNLDYLRSLHGKPLGNGPYVYDKYIPGQEIRFHANSHFY
RGTPPTPRFIYRVTNPSTNFQLFQTGETDYDAFTSRPDDIEQLKMLGFANINLYGSSDYSQVEFNVHRPALQDKRVRQAL
IYGLDRQKLIDVVYQGYGKVAIEPIAPISWAFNAEGVNPYPYDPAQAKKLLDEAGWKPGADGIRAKDGQRLELTLLVSKK
VLNDALIPIAKENWRQIGVLLKPQVVDFNALMAQRKAGNYDLASFSTSTLNDPHDGVWDFYSSEAKESGYHNAEVDKLIN
AGNAVLDIEQRKPIYHQLYKVLADDPPVILLGYREILSASSARVSGFKPDIYNGLTGSLPDVKIVK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=515, Percent_Identity=25.631067961165, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787052, Length=546, Percent_Identity=25.0915750915751, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787762, Length=517, Percent_Identity=26.6924564796905, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1789397, Length=476, Percent_Identity=26.0504201680672, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1789887, Length=485, Percent_Identity=24.5360824742268, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787551, Length=368, Percent_Identity=26.3586956521739, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787495, Length=513, Percent_Identity=24.1715399610136, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081878, Length=504, Percent_Identity=24.0079365079365, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59972; Mature: 59841

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLGGVVAFSSVAHAEVILKEGITPATDASQIPASAKLRKDTVVAGISEPQGIFNPYFF
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHCCCCEEEEECCCCCCCCCCEEE
VNGWDENVTNVIFSRLIDWDSQGKLVPGLAESWTVSPDSKVYTIKLRPGLTFSDGSPLTA
EECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCH
EDVAFTLTVLLDPSYDGDTDITLANIAGGADYKAGKADSVSGLKVIDPLTLQVTTTQPGA
HHEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCEEEEEEECCCCC
TTLAKIGGPVLSKAWYGKGYQRGNLDYLRSLHGKPLGNGPYVYDKYIPGQEIRFHANSHF
HHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCEEEECCCCE
YRGTPPTPRFIYRVTNPSTNFQLFQTGETDYDAFTSRPDDIEQLKMLGFANINLYGSSDY
ECCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHCCCCCHHHHHHHHCEEEEEEEECCCC
SQVEFNVHRPALQDKRVRQALIYGLDRQKLIDVVYQGYGKVAIEPIAPISWAFNAEGVNP
CEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCC
YPYDPAQAKKLLDEAGWKPGADGIRAKDGQRLELTLLVSKKVLNDALIPIAKENWRQIGV
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHCCCCCCCCHHHCCE
LLKPQVVDFNALMAQRKAGNYDLASFSTSTLNDPHDGVWDFYSSEAKESGYHNAEVDKLI
EECCEEECHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHH
NAGNAVLDIEQRKPIYHQLYKVLADDPPVILLGYREILSASSARVSGFKPDIYNGLTGSL
CCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCCCCCCHHHCCCCCCC
PDVKIVK
CCEEEEC
>Mature Secondary Structure 
ALLGGVVAFSSVAHAEVILKEGITPATDASQIPASAKLRKDTVVAGISEPQGIFNPYFF
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHCCCCEEEEECCCCCCCCCCEEE
VNGWDENVTNVIFSRLIDWDSQGKLVPGLAESWTVSPDSKVYTIKLRPGLTFSDGSPLTA
EECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCH
EDVAFTLTVLLDPSYDGDTDITLANIAGGADYKAGKADSVSGLKVIDPLTLQVTTTQPGA
HHEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCEEEEEEECCCCC
TTLAKIGGPVLSKAWYGKGYQRGNLDYLRSLHGKPLGNGPYVYDKYIPGQEIRFHANSHF
HHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCEEEECCCCE
YRGTPPTPRFIYRVTNPSTNFQLFQTGETDYDAFTSRPDDIEQLKMLGFANINLYGSSDY
ECCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHCCCCCHHHHHHHHCEEEEEEEECCCC
SQVEFNVHRPALQDKRVRQALIYGLDRQKLIDVVYQGYGKVAIEPIAPISWAFNAEGVNP
CEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCC
YPYDPAQAKKLLDEAGWKPGADGIRAKDGQRLELTLLVSKKVLNDALIPIAKENWRQIGV
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHCCCCCCCCHHHCCE
LLKPQVVDFNALMAQRKAGNYDLASFSTSTLNDPHDGVWDFYSSEAKESGYHNAEVDKLI
EECCEEECHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHH
NAGNAVLDIEQRKPIYHQLYKVLADDPPVILLGYREILSASSARVSGFKPDIYNGLTGSL
CCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCCCCCCHHHCCCCCCC
PDVKIVK
CCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]