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The map label for this gene is pcaB [H]

Identifier: 152970093

GI number: 152970093

Start: 1712115

End: 1713467

Strand: Direct

Name: pcaB [H]

Synonym: KPN_01541

Alternate gene names: 152970093

Gene position: 1712115-1713467 (Clockwise)

Preceding gene: 152970092

Following gene: 152970094

Centisome position: 32.21

GC content: 67.11

Gene sequence:

>1353_bases
ATGAGTTTGTTGACCCCGATGCTGCGCAGCAGTCCGCTAACCGACTGGTTCAGCGACGCGCAGCGGGTGCAGGGCATGCT
GGATTTTGAAGCGGCGCTGGCGCAAGCGCAGGCCGCCTGCGGGATGGTGCCGCCGGAAGCGGTCGGGCCAATTGTCGCGG
CCTGCCGCCACGAGGCCATCGATTTTGCCGCGCTGGGCGAGGCAGCGGTGGGAGCCGGTAACCTGGCGATCCCGCTAGTG
AAACAGCTGACGGCGCAGGTAAAAGCGCAAGATCCCGAGGCTGCGCGCTACGTTCACTGGGGCGCTACCAGCCAGGACGC
CATCGACACCGGACTGGTGCTCCAGCTGCGCGGGGCGCTGGATGCCGCTGAGACCCTGCTTGAGCAGCTGTTGGCGACCC
TGGCTCTTCAGGCGGATCGTTATCGCGATACCGTGATGCCGGGCCGGACCTGGATGCAGCACGCGCTGCCGGTGACCTTT
GGCCTGAAGCTGGCGGGCACCCTCGACGCGCTCCTGCGCTGGCAGCAGCGGCTGCGCGAGATGCGTCCGCGACTGCTGGC
GCTGCAGTTCGGCGGCGCCGCGGGCACTCTGGACGCGCTAAAAGAGAAAGGGCCGGCGGTTGGCCTGGCGCTGGCGCAGA
TCCTCGGTCTGTCGCTGCCGGATACTCCGTGGCACAGCCAGCGCGATCGGCTGCTGGAGGCGGGCGCCTGGTTCGCCGGC
GTCTGCGGCACGCTGGGGAAATTCGCTAACGATTTCTCCCTGCTGATGCAGACCGAAGTGGCTGAGGTCGGCGAGCCGGT
GGCGGAAGGCCGCGGCGGCTCGTCGACGATGCCGCATAAGCGCAATCCGGTGGCCTGCGCGGCGATCCTGACCGCCGCCC
AGCGTACGCCGGGGCTGATGGCGACGCTCTACGCCAGCCAACTCCAGCAGCATGAGCGGGCGCTGGGCGGCTGGCAGGCG
GAGTGGGAGACCCTGCCGGAGCTAATTACCCTGGTGGGCGGGGCGTTGGCGCAAAGCGAGGCGCTGGTGCGCGACATGCA
GGTTTTCCCGCAGAAAATGCGCGCCGATCTCGATATCACCCACGGGCTGATTATGGCTGAGGCGGTGACTCTGGCGCTGG
CGGAATTCATTGGCAAAGCCGAGGCGCATCATCACATCGAAGCGCTGTGCCGCCAGGCGCTGGACCGTCACTGCCCGCTG
GTCGATCTGCTCGCGGCTGACCCACAGGTCAGTCAGTATCTCTCTCGTGAACGCTTAACCACGCTGCTGGACCCGGCAAC
GGCGACCGGCAGCGCCGAACGCTTTGTGCGCCAGGTGCTGGCGCGTTATCAGGAGCAACGTGATGAATCTTGA

Upstream 100 bases:

>100_bases
TCAGCTGAAACGGACCGGCGGCCGCTATGCCCTGTGCACCATGTGCATCGGCGTCGGACAGGGGATCGCCCTGATTATTG
AACGCGTTTAAGGAGCCAGA

Downstream 100 bases:

>100_bases
TTATCAACTCGACGGGCCGGATGGCGCGCCGGTTATCGTCCTCTCCAATTCGCTGGGCACCACCCGGGCGATGTGGCAGC
CGCAGATCGAGGCGTTAACC

Product: putative fumarate lyase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSLLTPMLRSSPLTDWFSDAQRVQGMLDFEAALAQAQAACGMVPPEAVGPIVAACRHEAIDFAALGEAAVGAGNLAIPLV
KQLTAQVKAQDPEAARYVHWGATSQDAIDTGLVLQLRGALDAAETLLEQLLATLALQADRYRDTVMPGRTWMQHALPVTF
GLKLAGTLDALLRWQQRLREMRPRLLALQFGGAAGTLDALKEKGPAVGLALAQILGLSLPDTPWHSQRDRLLEAGAWFAG
VCGTLGKFANDFSLLMQTEVAEVGEPVAEGRGGSSTMPHKRNPVACAAILTAAQRTPGLMATLYASQLQQHERALGGWQA
EWETLPELITLVGGALAQSEALVRDMQVFPQKMRADLDITHGLIMAEAVTLALAEFIGKAEAHHHIEALCRQALDRHCPL
VDLLAADPQVSQYLSRERLTTLLDPATATGSAERFVRQVLARYQEQRDES

Sequences:

>Translated_450_residues
MSLLTPMLRSSPLTDWFSDAQRVQGMLDFEAALAQAQAACGMVPPEAVGPIVAACRHEAIDFAALGEAAVGAGNLAIPLV
KQLTAQVKAQDPEAARYVHWGATSQDAIDTGLVLQLRGALDAAETLLEQLLATLALQADRYRDTVMPGRTWMQHALPVTF
GLKLAGTLDALLRWQQRLREMRPRLLALQFGGAAGTLDALKEKGPAVGLALAQILGLSLPDTPWHSQRDRLLEAGAWFAG
VCGTLGKFANDFSLLMQTEVAEVGEPVAEGRGGSSTMPHKRNPVACAAILTAAQRTPGLMATLYASQLQQHERALGGWQA
EWETLPELITLVGGALAQSEALVRDMQVFPQKMRADLDITHGLIMAEAVTLALAEFIGKAEAHHHIEALCRQALDRHCPL
VDLLAADPQVSQYLSRERLTTLLDPATATGSAERFVRQVLARYQEQRDES
>Mature_449_residues
SLLTPMLRSSPLTDWFSDAQRVQGMLDFEAALAQAQAACGMVPPEAVGPIVAACRHEAIDFAALGEAAVGAGNLAIPLVK
QLTAQVKAQDPEAARYVHWGATSQDAIDTGLVLQLRGALDAAETLLEQLLATLALQADRYRDTVMPGRTWMQHALPVTFG
LKLAGTLDALLRWQQRLREMRPRLLALQFGGAAGTLDALKEKGPAVGLALAQILGLSLPDTPWHSQRDRLLEAGAWFAGV
CGTLGKFANDFSLLMQTEVAEVGEPVAEGRGGSSTMPHKRNPVACAAILTAAQRTPGLMATLYASQLQQHERALGGWQAE
WETLPELITLVGGALAQSEALVRDMQVFPQKMRADLDITHGLIMAEAVTLALAEFIGKAEAHHHIEALCRQALDRHCPLV
DLLAADPQVSQYLSRERLTTLLDPATATGSAERFVRQVLARYQEQRDES

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Escherichia coli, GI1790398, Length=232, Percent_Identity=30.1724137931034, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=376, Percent_Identity=26.063829787234, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI78710009, Length=344, Percent_Identity=24.7093023255814, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24647570, Length=392, Percent_Identity=27.2959183673469, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 48543; Mature: 48412

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLTPMLRSSPLTDWFSDAQRVQGMLDFEAALAQAQAACGMVPPEAVGPIVAACRHEAI
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DFAALGEAAVGAGNLAIPLVKQLTAQVKAQDPEAARYVHWGATSQDAIDTGLVLQLRGAL
HHHHHCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHH
DAAETLLEQLLATLALQADRYRDTVMPGRTWMQHALPVTFGLKLAGTLDALLRWQQRLRE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MRPRLLALQFGGAAGTLDALKEKGPAVGLALAQILGLSLPDTPWHSQRDRLLEAGAWFAG
HCHHEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHH
VCGTLGKFANDFSLLMQTEVAEVGEPVAEGRGGSSTMPHKRNPVACAAILTAAQRTPGLM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ATLYASQLQQHERALGGWQAEWETLPELITLVGGALAQSEALVRDMQVFPQKMRADLDIT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
HGLIMAEAVTLALAEFIGKAEAHHHIEALCRQALDRHCPLVDLLAADPQVSQYLSRERLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
TLLDPATATGSAERFVRQVLARYQEQRDES
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLLTPMLRSSPLTDWFSDAQRVQGMLDFEAALAQAQAACGMVPPEAVGPIVAACRHEAI
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DFAALGEAAVGAGNLAIPLVKQLTAQVKAQDPEAARYVHWGATSQDAIDTGLVLQLRGAL
HHHHHCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHH
DAAETLLEQLLATLALQADRYRDTVMPGRTWMQHALPVTFGLKLAGTLDALLRWQQRLRE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MRPRLLALQFGGAAGTLDALKEKGPAVGLALAQILGLSLPDTPWHSQRDRLLEAGAWFAG
HCHHEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHH
VCGTLGKFANDFSLLMQTEVAEVGEPVAEGRGGSSTMPHKRNPVACAAILTAAQRTPGLM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ATLYASQLQQHERALGGWQAEWETLPELITLVGGALAQSEALVRDMQVFPQKMRADLDIT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
HGLIMAEAVTLALAEFIGKAEAHHHIEALCRQALDRHCPLVDLLAADPQVSQYLSRERLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
TLLDPATATGSAERFVRQVLARYQEQRDES
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]