BacMap

Definition	Klebsiella pneumoniae subsp. pneumoniae MGH 78578 chromosome, complete genome.
Accession	NC_009648
Length	5,315,120

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The map label for this gene is paaG [H]

Identifier: 152970028

GI number: 152970028

Start: 1637797

End: 1638585

Strand: Direct

Name: paaG [H]

Synonym: KPN_01475

Alternate gene names: 152970028

Gene position: 1637797-1638585 (Clockwise)

Preceding gene: 152970027

Following gene: 152970029

Centisome position: 30.81

GC content: 64.39

Gene sequence:

>789_bases
GTGGAAGCTTTCATTCTCAGCGCAGTGGAACAGGGCGTCATGACCATTACCCTCAATCGCCCGGACCGCCTCAACAGTTT
TAACGACCTGATGCATCACCAGCTGGCGGAATGTCTGAAGCAGGCCGAGCGCGATGATGGCGTGCGCTGCCTGCTGATCA
CCGGCGCCGGTCGCGGCTTTTGCGCCGGCCAGGATTTGAACGACCGCAATGTCGACCCCAGCGGCCCGCCGCCGGATCTC
GGCATGTCGGTAGAACGCTTTTACAACCCGCTGGTACGCCGTCTGGCGGCGCTGCCGAAACCGGTCATCTGCGCGGTTAA
CGGCGTGGCCGCCGGCGCGGGCGCTACCCTGGCGCTGGGTTGCGATATCGTGCTGGCCGCGCGTTCGGCCAAATTTGTGA
TGGCGTTCAGCAAGCTCGGCCTGGTGCCGGACTGCGGCGGCAGCTGGTTCCTGCCGCGGGTGGCCGGCCGCGCTCGCGCG
ATGGGGCTGGCGCTGCTGGGCGACAGCCTCAGCGCAGAACAGGCCACGCAGTGGGGGATTATCTGGCAACTGGTGGACGA
TTCAGAACTGGCGGATACCAGTCTGCAACTGGCGCGCCATCTGGCGGCGCAACCGACGTTTGGTCTTGGGCTGATTAAAA
AGGCGCTGCTGGCCTCTGAAACCAACAGTCTCGACGCGCAGCTGGACCTGGAGCGCGATTATCAGCGTCTGGCCGGGCGC
AGCGATGATTACCGCGAAGGGGTCAGCGCGTTTCTGGCCAAACGGCCACCACAGTTCAGCGGGAAATAG

Upstream 100 bases:

>100_bases
AGCGCCAGCTGTTTAGCCTGCTGAGCGCCACCGAGGACCGCCGCGAAGGCATCGACGCCTTCTTACAAAAACGTACCCCG
GAATTTAAAGGACGCTAATC

Downstream 100 bases:

>100_bases
ATGATGACGACATCCTTAGCCACCGTGGCGGTGATCGGCAGCGGCACCATGGGGGCCGGCATCGCCGAAGTGGCTGCCGC
CGCCGGACATCCGGTGCGTA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MEAFILSAVEQGVMTITLNRPDRLNSFNDLMHHQLAECLKQAERDDGVRCLLITGAGRGFCAGQDLNDRNVDPSGPPPDL
GMSVERFYNPLVRRLAALPKPVICAVNGVAAGAGATLALGCDIVLAARSAKFVMAFSKLGLVPDCGGSWFLPRVAGRARA
MGLALLGDSLSAEQATQWGIIWQLVDDSELADTSLQLARHLAAQPTFGLGLIKKALLASETNSLDAQLDLERDYQRLAGR
SDDYREGVSAFLAKRPPQFSGK

Sequences:

>Translated_262_residues
MEAFILSAVEQGVMTITLNRPDRLNSFNDLMHHQLAECLKQAERDDGVRCLLITGAGRGFCAGQDLNDRNVDPSGPPPDL
GMSVERFYNPLVRRLAALPKPVICAVNGVAAGAGATLALGCDIVLAARSAKFVMAFSKLGLVPDCGGSWFLPRVAGRARA
MGLALLGDSLSAEQATQWGIIWQLVDDSELADTSLQLARHLAAQPTFGLGLIKKALLASETNSLDAQLDLERDYQRLAGR
SDDYREGVSAFLAKRPPQFSGK
>Mature_262_residues
MEAFILSAVEQGVMTITLNRPDRLNSFNDLMHHQLAECLKQAERDDGVRCLLITGAGRGFCAGQDLNDRNVDPSGPPPDL
GMSVERFYNPLVRRLAALPKPVICAVNGVAAGAGATLALGCDIVLAARSAKFVMAFSKLGLVPDCGGSWFLPRVAGRARA
MGLALLGDSLSAEQATQWGIIWQLVDDSELADTSLQLARHLAAQPTFGLGLIKKALLASETNSLDAQLDLERDYQRLAGR
SDDYREGVSAFLAKRPPQFSGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=30.9236947791165, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI45643119, Length=257, Percent_Identity=28.7937743190661, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI260275230, Length=257, Percent_Identity=28.7937743190661, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI260274832, Length=257, Percent_Identity=28.7937743190661, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI70995211, Length=258, Percent_Identity=27.906976744186, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=30.3501945525292, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI157694516, Length=231, Percent_Identity=26.8398268398268, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI213417737, Length=232, Percent_Identity=28.448275862069, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=27.9527559055118, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI37594469, Length=178, Percent_Identity=31.4606741573034, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI37594471, Length=178, Percent_Identity=31.4606741573034, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4757968, Length=189, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI49355787, Length=189, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI68989263, Length=190, Percent_Identity=28.4210526315789, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI25453481, Length=189, Percent_Identity=28.042328042328, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI51538573, Length=189, Percent_Identity=28.042328042328, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI4757966, Length=189, Percent_Identity=28.042328042328, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI51538581, Length=189, Percent_Identity=28.042328042328, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=26.775956284153, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221307494, Length=168, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221136756, Length=168, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221136753, Length=168, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI157694520, Length=206, Percent_Identity=25.7281553398058, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=84.3511450381679, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=37.4045801526718, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=31.0344827586207, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=27.4809160305344, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=29.1828793774319, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=27.8606965174129, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790281, Length=199, Percent_Identity=26.6331658291457, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=31.6, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=29.6442687747036, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=30.6034482758621, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=25.2918287937743, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=26.0869565217391, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=202, Percent_Identity=27.2277227722772, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=185, Percent_Identity=30.8108108108108, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=258, Percent_Identity=27.5193798449612, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=25.7692307692308, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=188, Percent_Identity=30.3191489361702, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=188, Percent_Identity=30.3191489361702, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=168, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=195, Percent_Identity=25.6410256410256, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=30.952380952381, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=30.952380952381, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=31.203007518797, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24654903, Length=205, Percent_Identity=29.7560975609756, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=26.0536398467433, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=28.1746031746032, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21357171, Length=223, Percent_Identity=27.8026905829596, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28571730, Length=178, Percent_Identity=30.8988764044944, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571729, Length=178, Percent_Identity=30.8988764044944, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=25.8215962441315, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28107; Mature: 28107

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAFILSAVEQGVMTITLNRPDRLNSFNDLMHHQLAECLKQAERDDGVRCLLITGAGRGF
CCHHHHHHHHCCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCE
CAGQDLNDRNVDPSGPPPDLGMSVERFYNPLVRRLAALPKPVICAVNGVAAGAGATLALG
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEC
CDIVLAARSAKFVMAFSKLGLVPDCGGSWFLPRVAGRARAMGLALLGDSLSAEQATQWGI
CCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCE
IWQLVDDSELADTSLQLARHLAAQPTFGLGLIKKALLASETNSLDAQLDLERDYQRLAGR
EEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECHHHHHHHHCCC
SDDYREGVSAFLAKRPPQFSGK
CCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEAFILSAVEQGVMTITLNRPDRLNSFNDLMHHQLAECLKQAERDDGVRCLLITGAGRGF
CCHHHHHHHHCCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCE
CAGQDLNDRNVDPSGPPPDLGMSVERFYNPLVRRLAALPKPVICAVNGVAAGAGATLALG
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEC
CDIVLAARSAKFVMAFSKLGLVPDCGGSWFLPRVAGRARAMGLALLGDSLSAEQATQWGI
CCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCE
IWQLVDDSELADTSLQLARHLAAQPTFGLGLIKKALLASETNSLDAQLDLERDYQRLAGR
EEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECHHHHHHHHCCC
SDDYREGVSAFLAKRPPQFSGK
CCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]