BacMap

Definition	Klebsiella pneumoniae subsp. pneumoniae MGH 78578 chromosome, complete genome.
Accession	NC_009648
Length	5,315,120

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The map label for this gene is paaF [H]

Identifier: 152970027

GI number: 152970027

Start: 1637027

End: 1637794

Strand: Direct

Name: paaF [H]

Synonym: KPN_01474

Alternate gene names: 152970027

Gene position: 1637027-1637794 (Clockwise)

Preceding gene: 152970026

Following gene: 152970028

Centisome position: 30.8

GC content: 62.63

Gene sequence:

>768_bases
ATGAGTGACCTGCTGATCCACCGTCATGGCCGGGTGCTTCAGTTGACGCTGAACCGCCCGCAGGCCCGCAACGCGCTGAA
CAATGCCTTATTAACCCAGATTGCCGAGGCGCTGGAGGCCGCGGCAGTTGATGACAGCGTCGGCGTCTGCGTTATTCGCG
GTAACGCGCGTTTTTTCGCCGCCGGGGCTGACCTTAATGAAATGGCGGAAAAAGATCTGCCTGCCACCCTGGATGATATT
CGCCCACGGTTATGGGGGCGAATTGATGCCTTTACTAAACCGCTCATCGCCTCGGTCAACGGCTACGCCCTCGGCGCCGG
CTGCGAGCTGGCCCTGCTGTGCGACCTGATTGTCGCCGGGGACAACGCCCGCTTTGGCCTGCCGGAAATTACCCTCGGCA
TAATGCCGGGCGCGGGCGGCACCCAGCGGCTGATCCGCAGCGTTGGCAAGGCGCTGGCCAGCCGGATGGTGCTGAGCGGC
GAGAGTATTGACGCCCGCCAGGCGCAGCAGGCCGGGCTGGTCAGTGATATCCATCCGGCGGCGTTAACCGATGAGTATGC
CCTGAAACTGGCAACCACCATTGCCCGCCATTCGCCGCTGGCGCTACGGGCGGCAAAACAGTCGCTGCGCCTGTCGCAGG
AAGTCAGCCTGCAGGCTGGTCTGCAACAGGAGCGCCAGCTGTTTAGCCTGCTGAGCGCCACCGAGGACCGCCGCGAAGGC
ATCGACGCCTTCTTACAAAAACGTACCCCGGAATTTAAAGGACGCTAA

Upstream 100 bases:

>100_bases
GCCTGGAAGCGGATGAGCTGGCGGCGGGCTACGTGCTGAGCTGCCAGTCGCTGCCGACCAGCGGCGATGTGGTGGTCGAC
TTTGACGCGCGGGGGATGGC

Downstream 100 bases:

>100_bases
TCGTGGAAGCTTTCATTCTCAGCGCAGTGGAACAGGGCGTCATGACCATTACCCTCAATCGCCCGGACCGCCTCAACAGT
TTTAACGACCTGATGCATCA

Product: enoyl-CoA hydratase-isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSDLLIHRHGRVLQLTLNRPQARNALNNALLTQIAEALEAAAVDDSVGVCVIRGNARFFAAGADLNEMAEKDLPATLDDI
RPRLWGRIDAFTKPLIASVNGYALGAGCELALLCDLIVAGDNARFGLPEITLGIMPGAGGTQRLIRSVGKALASRMVLSG
ESIDARQAQQAGLVSDIHPAALTDEYALKLATTIARHSPLALRAAKQSLRLSQEVSLQAGLQQERQLFSLLSATEDRREG
IDAFLQKRTPEFKGR

Sequences:

>Translated_255_residues
MSDLLIHRHGRVLQLTLNRPQARNALNNALLTQIAEALEAAAVDDSVGVCVIRGNARFFAAGADLNEMAEKDLPATLDDI
RPRLWGRIDAFTKPLIASVNGYALGAGCELALLCDLIVAGDNARFGLPEITLGIMPGAGGTQRLIRSVGKALASRMVLSG
ESIDARQAQQAGLVSDIHPAALTDEYALKLATTIARHSPLALRAAKQSLRLSQEVSLQAGLQQERQLFSLLSATEDRREG
IDAFLQKRTPEFKGR
>Mature_254_residues
SDLLIHRHGRVLQLTLNRPQARNALNNALLTQIAEALEAAAVDDSVGVCVIRGNARFFAAGADLNEMAEKDLPATLDDIR
PRLWGRIDAFTKPLIASVNGYALGAGCELALLCDLIVAGDNARFGLPEITLGIMPGAGGTQRLIRSVGKALASRMVLSGE
SIDARQAQQAGLVSDIHPAALTDEYALKLATTIARHSPLALRAAKQSLRLSQEVSLQAGLQQERQLFSLLSATEDRREGI
DAFLQKRTPEFKGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=44.3089430894309, Blast_Score=212, Evalue=3e-55,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=35.8565737051793, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=30.8943089430894, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=31.8897637795276, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI45643119, Length=254, Percent_Identity=26.7716535433071, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI260275230, Length=254, Percent_Identity=26.7716535433071, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI260274832, Length=254, Percent_Identity=26.7716535433071, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI25453481, Length=258, Percent_Identity=26.3565891472868, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI51538573, Length=258, Percent_Identity=26.3565891472868, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI70995211, Length=254, Percent_Identity=29.1338582677165, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI20127408, Length=206, Percent_Identity=33.495145631068, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4757966, Length=252, Percent_Identity=26.984126984127, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI51538581, Length=252, Percent_Identity=26.984126984127, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4757968, Length=258, Percent_Identity=25.5813953488372, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI49355787, Length=258, Percent_Identity=25.5813953488372, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI68989263, Length=154, Percent_Identity=37.012987012987, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI221136756, Length=259, Percent_Identity=25.4826254826255, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI221136753, Length=259, Percent_Identity=25.4826254826255, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI221307494, Length=259, Percent_Identity=25.4826254826255, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI157694516, Length=221, Percent_Identity=27.6018099547511, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI213417737, Length=221, Percent_Identity=27.6018099547511, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI116235487, Length=236, Percent_Identity=25.8474576271186, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI37594469, Length=181, Percent_Identity=27.0718232044199, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694520, Length=184, Percent_Identity=27.1739130434783, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594471, Length=181, Percent_Identity=27.0718232044199, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=79.2156862745098, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=36.1538461538462, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=32.5301204819277, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=33.7164750957854, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082183, Length=253, Percent_Identity=26.0869565217391, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790281, Length=172, Percent_Identity=33.7209302325581, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788682, Length=162, Percent_Identity=37.037037037037, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=241, Percent_Identity=46.0580912863071, Blast_Score=223, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI25145438, Length=238, Percent_Identity=45.3781512605042, Blast_Score=211, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=31.1111111111111, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=242, Percent_Identity=30.5785123966942, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=215, Percent_Identity=33.4883720930233, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=28.740157480315, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=262, Percent_Identity=29.0076335877863, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=210, Percent_Identity=32.3809523809524, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=229, Percent_Identity=27.9475982532751, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=147, Percent_Identity=34.0136054421769, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17508951, Length=147, Percent_Identity=34.0136054421769, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17508953, Length=147, Percent_Identity=34.0136054421769, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=251, Percent_Identity=27.0916334661355, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320241, Length=176, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129971, Length=238, Percent_Identity=44.5378151260504, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24653477, Length=238, Percent_Identity=44.5378151260504, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24653139, Length=248, Percent_Identity=35.4838709677419, Blast_Score=137, Evalue=6e-33,
Organism=Drosophila melanogaster, GI21357171, Length=254, Percent_Identity=29.5275590551181, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19920382, Length=254, Percent_Identity=29.5275590551181, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=25.5813953488372, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24650670, Length=265, Percent_Identity=28.6792452830189, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24654903, Length=183, Percent_Identity=27.3224043715847, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583165, Length=239, Percent_Identity=25.5230125523013, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921018, Length=216, Percent_Identity=27.3148148148148, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27411; Mature: 27280

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLIHRHGRVLQLTLNRPQARNALNNALLTQIAEALEAAAVDDSVGVCVIRGNARFFA
CCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEE
AGADLNEMAEKDLPATLDDIRPRLWGRIDAFTKPLIASVNGYALGAGCELALLCDLIVAG
ECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCC
DNARFGLPEITLGIMPGAGGTQRLIRSVGKALASRMVLSGESIDARQAQQAGLVSDIHPA
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHCCCCH
ALTDEYALKLATTIARHSPLALRAAKQSLRLSQEVSLQAGLQQERQLFSLLSATEDRREG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDAFLQKRTPEFKGR
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDLLIHRHGRVLQLTLNRPQARNALNNALLTQIAEALEAAAVDDSVGVCVIRGNARFFA
CCCEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEE
AGADLNEMAEKDLPATLDDIRPRLWGRIDAFTKPLIASVNGYALGAGCELALLCDLIVAG
ECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCC
DNARFGLPEITLGIMPGAGGTQRLIRSVGKALASRMVLSGESIDARQAQQAGLVSDIHPA
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHCCCCH
ALTDEYALKLATTIARHSPLALRAAKQSLRLSQEVSLQAGLQQERQLFSLLSATEDRREG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDAFLQKRTPEFKGR
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]