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The map label for this gene is mipA [H]

Identifier: 152969748

GI number: 152969748

Start: 1344308

End: 1345054

Strand: Direct

Name: mipA [H]

Synonym: KPN_01194

Alternate gene names: 152969748

Gene position: 1344308-1345054 (Clockwise)

Preceding gene: 152969745

Following gene: 152969749

Centisome position: 25.29

GC content: 55.82

Gene sequence:

>747_bases
GTGACTAAAATCAAACTTCTGGCACTCGGCGTGCTGATAGCGATGTCCGCCAGCGCGGCGCATGCCGACGGGAAGTTCAC
TCTTGGGGCTGGCGTAGGGGTCGTTGAGCATCCGTATAAGCAATATGATGCGGATGTCTATCCGGTGCCGGTCATTAGTT
ATGAGAGCGAAAACTTCTGGTTCCACGGTCTGGGCGGGGGTTACTACCTGTGGAATGACACCAACGATAAACTGTCGATT
ACGGCCTACTGGTCGCCAATGTACTTCAAGCCGGGCGACAGCGACAGCGAGCAGATGCGTCGACTGGATAAACGTAAATC
GACCGTGATGGCCGGTCTTTCTTATGTCCACAACACGCCCTATGGCTTCCTGCGCACGACGATTGCCGGCGATACGCTGG
ATAACAGCAACGGTATTAACTGGGATCTCGCCTGGCTGTACCGTTACACCAACGGTAACCTGACGCTGACGCCGGGTATT
GGCGTGGAGTGGAACAGCGACAATCAGAACGAATACTACTATGGTGTCTCCCGGCACGAGTCGCGCCGCAGCGGCATGCG
CAGCTATGACCCGGACAGTAGCTGGAACCCGTATCTGGAACTGTCGGCTAACTATCGCTTCCTTGGTGACTGGAGCGTTT
ACGGCGTGGCGCGCTACACGCGTCTGTCGGATGAAATTACCGACAGTCCGATGGTGGATAAATCCTGGAGCGGTCTGATC
TCCACCGGGATCACCTACACCTTCTGA

Upstream 100 bases:

>100_bases
AAAGTGAACGATTTGTGTCATATCGGGTAAAGAATTTGAGGCATGAGACCTACGACGGTACACTGCACGGGCTGATTATT
TGACAACGGGAATATGGATT

Downstream 100 bases:

>100_bases
TTGTGCATTCTTACAGTGCGCTTCATGCAGCAAAACAGGGCCTTGCGCCCTGTTTTGCTTTATGGTGGTGCATACGGTTA
AGGAGGAGAAGATGGTGAAA

Product: scaffolding protein for murein-synthesising holoenzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTKIKLLALGVLIAMSASAAHADGKFTLGAGVGVVEHPYKQYDADVYPVPVISYESENFWFHGLGGGYYLWNDTNDKLSI
TAYWSPMYFKPGDSDSEQMRRLDKRKSTVMAGLSYVHNTPYGFLRTTIAGDTLDNSNGINWDLAWLYRYTNGNLTLTPGI
GVEWNSDNQNEYYYGVSRHESRRSGMRSYDPDSSWNPYLELSANYRFLGDWSVYGVARYTRLSDEITDSPMVDKSWSGLI
STGITYTF

Sequences:

>Translated_248_residues
MTKIKLLALGVLIAMSASAAHADGKFTLGAGVGVVEHPYKQYDADVYPVPVISYESENFWFHGLGGGYYLWNDTNDKLSI
TAYWSPMYFKPGDSDSEQMRRLDKRKSTVMAGLSYVHNTPYGFLRTTIAGDTLDNSNGINWDLAWLYRYTNGNLTLTPGI
GVEWNSDNQNEYYYGVSRHESRRSGMRSYDPDSSWNPYLELSANYRFLGDWSVYGVARYTRLSDEITDSPMVDKSWSGLI
STGITYTF
>Mature_247_residues
TKIKLLALGVLIAMSASAAHADGKFTLGAGVGVVEHPYKQYDADVYPVPVISYESENFWFHGLGGGYYLWNDTNDKLSIT
AYWSPMYFKPGDSDSEQMRRLDKRKSTVMAGLSYVHNTPYGFLRTTIAGDTLDNSNGINWDLAWLYRYTNGNLTLTPGIG
VEWNSDNQNEYYYGVSRHESRRSGMRSYDPDSSWNPYLELSANYRFLGDWSVYGVARYTRLSDEITDSPMVDKSWSGLIS
TGITYTF

Specific function: May serve as a scaffold protein required for the formation of a complex with mrcB/ponB and mltA, this complex could play a role in enlargement and septation of the murein sacculus [H]

COG id: COG3713

COG function: function code M; Outer membrane protein V

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mipA/ompV family [H]

Homologues:

Organism=Escherichia coli, GI1788082, Length=248, Percent_Identity=80.6451612903226, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1790010, Length=238, Percent_Identity=38.2352941176471, Blast_Score=180, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010583 [H]

Pfam domain/function: PF06629 MipA [H]

EC number: NA

Molecular weight: Translated: 28068; Mature: 27937

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIKLLALGVLIAMSASAAHADGKFTLGAGVGVVEHPYKQYDADVYPVPVISYESENFW
CCEEEHHHHHHHHHHCCCCCCCCCCEEEECCCCHHCCCHHHHCCCCCCEEEEEECCCCCE
FHGLGGGYYLWNDTNDKLSITAYWSPMYFKPGDSDSEQMRRLDKRKSTVMAGLSYVHNTP
EEECCCCEEEECCCCCEEEEEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
YGFLRTTIAGDTLDNSNGINWDLAWLYRYTNGNLTLTPGIGVEWNSDNQNEYYYGVSRHE
CCEEEEEECCCCCCCCCCCCEEEEEEEEEECCCEEECCCCCEEECCCCCCEEEEECCHHH
SRRSGMRSYDPDSSWNPYLELSANYRFLGDWSVYGVARYTRLSDEITDSPMVDKSWSGLI
HHHCCCCCCCCCCCCCCEEEEECCEEEEECEEEEEEEEEECCCHHCCCCCCCCCCCCCHH
STGITYTF
HCCEEEEC
>Mature Secondary Structure 
TKIKLLALGVLIAMSASAAHADGKFTLGAGVGVVEHPYKQYDADVYPVPVISYESENFW
CEEEHHHHHHHHHHCCCCCCCCCCEEEECCCCHHCCCHHHHCCCCCCEEEEEECCCCCE
FHGLGGGYYLWNDTNDKLSITAYWSPMYFKPGDSDSEQMRRLDKRKSTVMAGLSYVHNTP
EEECCCCEEEECCCCCEEEEEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
YGFLRTTIAGDTLDNSNGINWDLAWLYRYTNGNLTLTPGIGVEWNSDNQNEYYYGVSRHE
CCEEEEEECCCCCCCCCCCCEEEEEEEEEECCCEEECCCCCEEECCCCCCEEEEECCHHH
SRRSGMRSYDPDSSWNPYLELSANYRFLGDWSVYGVARYTRLSDEITDSPMVDKSWSGLI
HHHCCCCCCCCCCCCCCEEEEECCEEEEECEEEEEEEEEECCCHHCCCCCCCCCCCCCHH
STGITYTF
HCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 10037771; 10806384 [H]