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The map label for this gene is bioD [H]

Identifier: 152969360

GI number: 152969360

Start: 896890

End: 897612

Strand: Direct

Name: bioD [H]

Synonym: KPN_00803

Alternate gene names: 152969360

Gene position: 896890-897612 (Clockwise)

Preceding gene: 152969359

Following gene: 152969361

Centisome position: 16.87

GC content: 63.76

Gene sequence:

>723_bases
GTGACTAAACGTTTTTTTGTCACCGGGACCGATACTGAAGTCGGTAAAACCATCGCCAGCAGCGCCCTGCTGCAGGCGGC
GAATCTGCTGGGTTTTCACACTGCCGGCTATAAGCCGGTGGCCTCCGGTAGCGAGCTTACCGGCGATGGGCTGCGTAATG
AAGATGCGCTGGCGCTGCAGCGTCATAGTCGGGTCGCATTGCGTTACGACCAGGTGAACCCCTATACCTTCGCCGAGCCG
ACCTCCCCGCACATCATCAGCGCCGACGAAGGGCGGCCGATCGCCGCGGCCAGGCTCTCCAGCGGGCTGCGTGAGCTGGA
AGGTCTCGCCGACTGGGTGTTGGTGGAAGGCGCCGGCGGCTGGTTTACGCCGCTCTCGGATTCCCTGACCTTCGCCGACT
GGGCGCAGGCGGAACAGCTGCCGGTCATTCTGGTGGTGGGCGTTAAGCTCGGCTGTATCAACCACGCAATGCTTACCGCT
CAGGCGGTACGCCAGGCGGGACTACCGCTGGCGGGGTGGATCGCCAATGACGTACAGCCGCCTGGCAAGCGCCACGCCGA
ATATCTGGCGACGCTGAAAAACCGACTGGCCGCGCCGTTCCTCGGCGAAATACCGTGGCTTGCCGATATCGCGCAGCGCG
ACGATCTCGGCCAGTATCTGGACCTCAGGGCGCTGGATCCGGCGTTATCCACTGCGCCAGCAGCGGCTCATCCAGCGCCA
TAA

Upstream 100 bases:

>100_bases
CCGCTTGGCCGCGGCAAACTGCGCGAGCTGCAGCTGGCCTGGCCGCAGCGGCAGGGCATCTGCCCGCTGACGTATTCTCT
TTTTACAGGAGTCATTGAGC

Downstream 100 bases:

>100_bases
TATCGCCTTCGGCGACGTTACGGCCCCGGTGCAAGACACAGAAGCGATCCGCCACCCGGCGGATAAAGGGCAGGTGCTGC
TCGGCGAGCAGCACCGTCAG

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTKRFFVTGTDTEVGKTIASSALLQAANLLGFHTAGYKPVASGSELTGDGLRNEDALALQRHSRVALRYDQVNPYTFAEP
TSPHIISADEGRPIAAARLSSGLRELEGLADWVLVEGAGGWFTPLSDSLTFADWAQAEQLPVILVVGVKLGCINHAMLTA
QAVRQAGLPLAGWIANDVQPPGKRHAEYLATLKNRLAAPFLGEIPWLADIAQRDDLGQYLDLRALDPALSTAPAAAHPAP

Sequences:

>Translated_240_residues
MTKRFFVTGTDTEVGKTIASSALLQAANLLGFHTAGYKPVASGSELTGDGLRNEDALALQRHSRVALRYDQVNPYTFAEP
TSPHIISADEGRPIAAARLSSGLRELEGLADWVLVEGAGGWFTPLSDSLTFADWAQAEQLPVILVVGVKLGCINHAMLTA
QAVRQAGLPLAGWIANDVQPPGKRHAEYLATLKNRLAAPFLGEIPWLADIAQRDDLGQYLDLRALDPALSTAPAAAHPAP
>Mature_239_residues
TKRFFVTGTDTEVGKTIASSALLQAANLLGFHTAGYKPVASGSELTGDGLRNEDALALQRHSRVALRYDQVNPYTFAEPT
SPHIISADEGRPIAAARLSSGLRELEGLADWVLVEGAGGWFTPLSDSLTFADWAQAEQLPVILVVGVKLGCINHAMLTAQ
AVRQAGLPLAGWIANDVQPPGKRHAEYLATLKNRLAAPFLGEIPWLADIAQRDDLGQYLDLRALDPALSTAPAAAHPAP

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=72, Blast_Score=335, Evalue=1e-93,
Organism=Escherichia coli, GI87081944, Length=231, Percent_Identity=48.4848484848485, Blast_Score=210, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 25565; Mature: 25434

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRFFVTGTDTEVGKTIASSALLQAANLLGFHTAGYKPVASGSELTGDGLRNEDALALQ
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RHSRVALRYDQVNPYTFAEPTSPHIISADEGRPIAAARLSSGLRELEGLADWVLVEGAGG
HCCEEEEEECCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCC
WFTPLSDSLTFADWAQAEQLPVILVVGVKLGCINHAMLTAQAVRQAGLPLAGWIANDVQP
CCCCCCCCCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCC
PGKRHAEYLATLKNRLAAPFLGEIPWLADIAQRDDLGQYLDLRALDPALSTAPAAAHPAP
CHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCHHHHHHHHHCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
TKRFFVTGTDTEVGKTIASSALLQAANLLGFHTAGYKPVASGSELTGDGLRNEDALALQ
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RHSRVALRYDQVNPYTFAEPTSPHIISADEGRPIAAARLSSGLRELEGLADWVLVEGAGG
HCCEEEEEECCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCC
WFTPLSDSLTFADWAQAEQLPVILVVGVKLGCINHAMLTAQAVRQAGLPLAGWIANDVQP
CCCCCCCCCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCC
PGKRHAEYLATLKNRLAAPFLGEIPWLADIAQRDDLGQYLDLRALDPALSTAPAAAHPAP
CHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCHHHHHHHHHCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9063571 [H]