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The map label for this gene is bioF

Identifier: 152969358

GI number: 152969358

Start: 895001

End: 896158

Strand: Direct

Name: bioF

Synonym: KPN_00801

Alternate gene names: 152969358

Gene position: 895001-896158 (Clockwise)

Preceding gene: 152969357

Following gene: 152969359

Centisome position: 16.84

GC content: 67.62

Gene sequence:

>1158_bases
ATGAGCTGGCAGCAGCGAATCGATCGGGCGCTGGACGAGCGGCGGGCGGCGGAAGCTTTTCGCCGCCGCCTGCCGGTGAC
GCACGGCGCCGGGCGCTGGCTGGAGCGGGAAGGGGAGCGCTGGCTTAACTTCTCCAGCAATGACTATCTGGGCCTGAGTC
AGCATCCGGCGATTATCGCCGCCTGGCAACAGGGGGCCACGCGCTATGGCGTCGGCGCCGGCGGCTCGGGCCACGTGAGC
GGCTATAGCGAAGCCCACCGCGCGCTGGAAGAGGCGCTCGCCGACTGGCTGGGCTATCCGCGCGCATTGCTGTTTATCTC
CGGCTTTGCCGCGAATCAGGCGCTGATCGCCGCGCTGGCGGAAAAAGACGATCGCATTGTGGCCGATCGCCTGAGCCATG
CCTCCCTGCTGGAGGCGGCAAGCCTCAGCCCGGCGCAGCTGCGGCGCTTTACCCACAACGATCCGCAGCAGCTCGCGCAA
CTGCTGGCTAAGCCGCTGGCGGGCGAACAGTTGGCGGTCACTGAAGGCATTTTCAGCATGGACGGCGACAGCGCGCCGCT
GGCGGCCATTCACGCCGCGACGCAGGCCGCAGGGGCAGTGTTGCTGGTGGATGACGCCCACGGTGTTGGCGTGATCGGCG
ACGAAGGACGCGGGAGCTGCGCCGCGCAGGCGGTGCGCCCTGAGCTGCTGGTGGTTACCTTCGGCAAGGCCTTCGGCGTC
AGCGGCGCGGCGGTGCTGTGCGACGAAGCAATGGCCGATTATCTGCTGCAGTTTGCCCGTCATCTGATCTACAGCACCGC
GATGCCGCCCGCCCAGGCGGTGGCGCTGAGCGCTGCCCTGCGGATCATTCGCAGCGATGAAGGGCAGCAGCGTCGCGACA
TCCTGGCCGCCCGCATCCGTCAGTTCCGTGAGGGGATGGGCGAGGTGTCGCTGGGGTTGACCGACTCCGTCAGCGCGATT
CAGCCGCTGATCGTCGGCGACAACGCCCGGGCGCTGAACCTGGCCTGCCGTCTACGCGACGCGGGCTGCTGGGCAACGGC
GATCCGTCCACCGACGGTGCCGGTCGGCAGCGCGCGTTTGCGCCTGACCCTGACAGCCGCCCATCATACTGAAGACATTA
ACCGTTTACTGGAGGTGCTGCATGGCCACAGTGAATAA

Upstream 100 bases:

>100_bases
AGCAGACGGCGGTGCTGGAAGGCGACAATGAGCAGCAGCAGCGTCTGGAGCAGGCCTTACTTACCCCGGACACTGAGGAA
TATTACAACGCGGCGGCGCT

Downstream 100 bases:

>100_bases
GCAGGCGGTGGCCGCGGCGTTTGGCCGGGCGGCCAGCGGCTATACGCAACATGATGAACTGCAGCGGCGCTGCGCCGATC
TACTGCTGCGCCAGCTGGCG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSWQQRIDRALDERRAAEAFRRRLPVTHGAGRWLEREGERWLNFSSNDYLGLSQHPAIIAAWQQGATRYGVGAGGSGHVS
GYSEAHRALEEALADWLGYPRALLFISGFAANQALIAALAEKDDRIVADRLSHASLLEAASLSPAQLRRFTHNDPQQLAQ
LLAKPLAGEQLAVTEGIFSMDGDSAPLAAIHAATQAAGAVLLVDDAHGVGVIGDEGRGSCAAQAVRPELLVVTFGKAFGV
SGAAVLCDEAMADYLLQFARHLIYSTAMPPAQAVALSAALRIIRSDEGQQRRDILAARIRQFREGMGEVSLGLTDSVSAI
QPLIVGDNARALNLACRLRDAGCWATAIRPPTVPVGSARLRLTLTAAHHTEDINRLLEVLHGHSE

Sequences:

>Translated_385_residues
MSWQQRIDRALDERRAAEAFRRRLPVTHGAGRWLEREGERWLNFSSNDYLGLSQHPAIIAAWQQGATRYGVGAGGSGHVS
GYSEAHRALEEALADWLGYPRALLFISGFAANQALIAALAEKDDRIVADRLSHASLLEAASLSPAQLRRFTHNDPQQLAQ
LLAKPLAGEQLAVTEGIFSMDGDSAPLAAIHAATQAAGAVLLVDDAHGVGVIGDEGRGSCAAQAVRPELLVVTFGKAFGV
SGAAVLCDEAMADYLLQFARHLIYSTAMPPAQAVALSAALRIIRSDEGQQRRDILAARIRQFREGMGEVSLGLTDSVSAI
QPLIVGDNARALNLACRLRDAGCWATAIRPPTVPVGSARLRLTLTAAHHTEDINRLLEVLHGHSE
>Mature_384_residues
SWQQRIDRALDERRAAEAFRRRLPVTHGAGRWLEREGERWLNFSSNDYLGLSQHPAIIAAWQQGATRYGVGAGGSGHVSG
YSEAHRALEEALADWLGYPRALLFISGFAANQALIAALAEKDDRIVADRLSHASLLEAASLSPAQLRRFTHNDPQQLAQL
LAKPLAGEQLAVTEGIFSMDGDSAPLAAIHAATQAAGAVLLVDDAHGVGVIGDEGRGSCAAQAVRPELLVVTFGKAFGVS
GAAVLCDEAMADYLLQFARHLIYSTAMPPAQAVALSAALRIIRSDEGQQRRDILAARIRQFREGMGEVSLGLTDSVSAIQ
PLIVGDNARALNLACRLRDAGCWATAIRPPTVPVGSARLRLTLTAAHHTEDINRLLEVLHGHSE

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=346, Percent_Identity=32.0809248554913, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI284448556, Length=346, Percent_Identity=32.0809248554913, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI83977444, Length=345, Percent_Identity=31.8840579710145, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI83977442, Length=345, Percent_Identity=31.8840579710145, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI83977440, Length=345, Percent_Identity=31.8840579710145, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4502025, Length=395, Percent_Identity=29.620253164557, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI40316939, Length=395, Percent_Identity=29.620253164557, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI119220554, Length=368, Percent_Identity=29.3478260869565, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4758668, Length=361, Percent_Identity=29.3628808864266, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI5454084, Length=192, Percent_Identity=27.0833333333333, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786993, Length=383, Percent_Identity=71.5404699738903, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1790046, Length=386, Percent_Identity=33.9378238341969, Blast_Score=201, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI71994529, Length=345, Percent_Identity=32.463768115942, Blast_Score=184, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI32566772, Length=360, Percent_Identity=28.6111111111111, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17560914, Length=385, Percent_Identity=29.0909090909091, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17560912, Length=385, Percent_Identity=29.0909090909091, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71982625, Length=261, Percent_Identity=26.4367816091954, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982617, Length=261, Percent_Identity=26.4367816091954, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320438, Length=418, Percent_Identity=27.7511961722488, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320267, Length=375, Percent_Identity=26.6666666666667, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323954, Length=268, Percent_Identity=27.6119402985075, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24662918, Length=367, Percent_Identity=30.2452316076294, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI17137420, Length=355, Percent_Identity=30.7042253521127, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17136286, Length=363, Percent_Identity=27.8236914600551, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24653280, Length=265, Percent_Identity=24.9056603773585, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24653276, Length=265, Percent_Identity=24.9056603773585, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24653278, Length=265, Percent_Identity=24.9056603773585, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_KLEP7 (A6T6L6)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001334467.1
- ProteinModelPortal:   A6T6L6
- SMR:   A6T6L6
- STRING:   A6T6L6
- GeneID:   5340750
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_00801
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   KDYASND
- ProtClustDB:   PRK05958
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 41269; Mature: 41138

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 21-21 BINDING 133-133 BINDING 179-179 BINDING 352-352

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQQRIDRALDERRAAEAFRRRLPVTHGAGRWLEREGERWLNFSSNDYLGLSQHPAIIA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHCCCCCCCCCCCCCCCCEEE
AWQQGATRYGVGAGGSGHVSGYSEAHRALEEALADWLGYPRALLFISGFAANQALIAALA
EECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHH
EKDDRIVADRLSHASLLEAASLSPAQLRRFTHNDPQQLAQLLAKPLAGEQLAVTEGIFSM
HCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCEEHHHCHHCC
DGDSAPLAAIHAATQAAGAVLLVDDAHGVGVIGDEGRGSCAAQAVRPELLVVTFGKAFGV
CCCCCCHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCCHHHHHCCCEEEEEEECHHHCC
SGAAVLCDEAMADYLLQFARHLIYSTAMPPAQAVALSAALRIIRSDEGQQRRDILAARIR
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QFREGMGEVSLGLTDSVSAIQPLIVGDNARALNLACRLRDAGCWATAIRPPTVPVGSARL
HHHHCCCCEECCCCCCHHHHCCEEECCCCCEEEEEEEEECCCCEEECCCCCCCCCCCCEE
RLTLTAAHHTEDINRLLEVLHGHSE
EEEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SWQQRIDRALDERRAAEAFRRRLPVTHGAGRWLEREGERWLNFSSNDYLGLSQHPAIIA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHCCCCCCCCCCCCCCCCEEE
AWQQGATRYGVGAGGSGHVSGYSEAHRALEEALADWLGYPRALLFISGFAANQALIAALA
EECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHH
EKDDRIVADRLSHASLLEAASLSPAQLRRFTHNDPQQLAQLLAKPLAGEQLAVTEGIFSM
HCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCEEHHHCHHCC
DGDSAPLAAIHAATQAAGAVLLVDDAHGVGVIGDEGRGSCAAQAVRPELLVVTFGKAFGV
CCCCCCHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCCHHHHHCCCEEEEEEECHHHCC
SGAAVLCDEAMADYLLQFARHLIYSTAMPPAQAVALSAALRIIRSDEGQQRRDILAARIR
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QFREGMGEVSLGLTDSVSAIQPLIVGDNARALNLACRLRDAGCWATAIRPPTVPVGSARL
HHHHCCCCEECCCCCCHHHHCCEEECCCCCEEEEEEEEECCCCEEECCCCCCCCCCCCEE
RLTLTAAHHTEDINRLLEVLHGHSE
EEEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA