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The map label for this gene is ycgF [H]

Identifier: 152969339

GI number: 152969339

Start: 868538

End: 869749

Strand: Direct

Name: ycgF [H]

Synonym: KPN_00782

Alternate gene names: 152969339

Gene position: 868538-869749 (Clockwise)

Preceding gene: 152969338

Following gene: 152969340

Centisome position: 16.34

GC content: 51.4

Gene sequence:

>1212_bases
ATGCTAACCACCATCATTTATCGCAGCCATATCTGCGACAATGTCTCATTCAAATCGATCGAGGCCATGGTCGCCAGGGC
AAACGAAAGAAATGGGCAGGCGGACGTCACCGGTATTTTACTTTTTAACGGGACGCATTTTTTTCAACTCATTGAGGGGC
CTGAAGAAAAGGTTCAGGATATTTATCAGCATATCTGCCAGGATCCTCGCCACTATAATCTGGTGGAGTTGCTTTGCGAT
TATGCGCCGTCCCGTCGTTTTGGTAAGGTCGGGATGGAACTGTTTGATTTACGCGAACACGATCGCGAGGAAGTTCTGCA
GGCGGTGATGGACCGGGGGACGTCAAAATATCAGCTCACCTATGATGACCGGGCGCTGCAATTTTTCCGTACTTTTGTCG
AAGCCACAGAAAAAGCGAACTATTTTGAGATTCCTTCCGCAGATAGTTGGGTTTTTATTCCTGATAAGGAGACGTTCTAT
CCTGATACCCCGATTATCGATAATACGGAAGGTTGTTCCTTTGCCTTCCAGCCGATTGTCGACCCTTTCGCCTGCGAAAT
TATTTCGTGGGAAGCGCTGTTGCGTACACCGGACGGGCAATCCCCCGGCGCCTATTTTGCCGGGCTGACCGGGGACGATA
TTTATCTCGCCGACCTGCACAGTAAACGGGTGGCGTTGTCGCTGGCGGGAAAATTAGGTTTACGTAATAAGGCGTTAAGC
ATTAATTTACTGCCGATGACCATGGTCAAAGCGCCGAATGCGGTGGCTTTCCTGCTCGACGAAATTAGTCGCAATGACCT
TATTCCGGAGCAGATTATTGTCGAGTTCACTGAGCGGGAAGTGATTTCGCGGATGGACGATTTTACCGATGCGGTGCGCA
AACTGAAGGGCGCGGGAATTAATCTTGCCATCGATCACTTCGGCGCCGGGTTTGCCGGGCTATCGCTGCTGGCGCAATAC
CAACCCGACAGAATTAAAATCGACCATGAGCTGATACGCAATATCCACCAGGACGGACCGCGCCAGTCGATTGTACAGGC
GATTATCAAATGCTGTACTTCTCTGGAGATTGCGGTCTCAGCCGTCGGCGTTGAGCGTGCTGAAGAGTGGATGTGGCTGG
AATCAGCCGGTATTTCACAGTTTCAGGGCAATTTGTTCGCCAGCGCGCGCCTGGGGGGCCTACCCGCCGTCGCATGGCCG
GAGAAAAAATGA

Upstream 100 bases:

>100_bases
TTTTAGTGTATCTTTTTTGTTAAGACAAAACGTAGTTTTACGAGACCCTTAGCCTGATAACTGCTACTGTGAATGCTTAT
CCCTCCCATCTGGAATCACT

Downstream 100 bases:

>100_bases
ACCACCGCGAAATCAACTAAACATTTTTTCATCTTTTTTCCCGGGCGCTGTGAGCCGCAACCTGATATCTTTATCTGTAC
AAGAAAATAGAGTTGTACAG

Product: hypothetical protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MLTTIIYRSHICDNVSFKSIEAMVARANERNGQADVTGILLFNGTHFFQLIEGPEEKVQDIYQHICQDPRHYNLVELLCD
YAPSRRFGKVGMELFDLREHDREEVLQAVMDRGTSKYQLTYDDRALQFFRTFVEATEKANYFEIPSADSWVFIPDKETFY
PDTPIIDNTEGCSFAFQPIVDPFACEIISWEALLRTPDGQSPGAYFAGLTGDDIYLADLHSKRVALSLAGKLGLRNKALS
INLLPMTMVKAPNAVAFLLDEISRNDLIPEQIIVEFTEREVISRMDDFTDAVRKLKGAGINLAIDHFGAGFAGLSLLAQY
QPDRIKIDHELIRNIHQDGPRQSIVQAIIKCCTSLEIAVSAVGVERAEEWMWLESAGISQFQGNLFASARLGGLPAVAWP
EKK

Sequences:

>Translated_403_residues
MLTTIIYRSHICDNVSFKSIEAMVARANERNGQADVTGILLFNGTHFFQLIEGPEEKVQDIYQHICQDPRHYNLVELLCD
YAPSRRFGKVGMELFDLREHDREEVLQAVMDRGTSKYQLTYDDRALQFFRTFVEATEKANYFEIPSADSWVFIPDKETFY
PDTPIIDNTEGCSFAFQPIVDPFACEIISWEALLRTPDGQSPGAYFAGLTGDDIYLADLHSKRVALSLAGKLGLRNKALS
INLLPMTMVKAPNAVAFLLDEISRNDLIPEQIIVEFTEREVISRMDDFTDAVRKLKGAGINLAIDHFGAGFAGLSLLAQY
QPDRIKIDHELIRNIHQDGPRQSIVQAIIKCCTSLEIAVSAVGVERAEEWMWLESAGISQFQGNLFASARLGGLPAVAWP
EKK
>Mature_403_residues
MLTTIIYRSHICDNVSFKSIEAMVARANERNGQADVTGILLFNGTHFFQLIEGPEEKVQDIYQHICQDPRHYNLVELLCD
YAPSRRFGKVGMELFDLREHDREEVLQAVMDRGTSKYQLTYDDRALQFFRTFVEATEKANYFEIPSADSWVFIPDKETFY
PDTPIIDNTEGCSFAFQPIVDPFACEIISWEALLRTPDGQSPGAYFAGLTGDDIYLADLHSKRVALSLAGKLGLRNKALS
INLLPMTMVKAPNAVAFLLDEISRNDLIPEQIIVEFTEREVISRMDDFTDAVRKLKGAGINLAIDHFGAGFAGLSLLAQY
QPDRIKIDHELIRNIHQDGPRQSIVQAIIKCCTSLEIAVSAVGVERAEEWMWLESAGISQFQGNLFASARLGGLPAVAWP
EKK

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=404, Percent_Identity=65.5940594059406, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI226510982, Length=243, Percent_Identity=32.0987654320988, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=128, Percent_Identity=36.71875, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790496, Length=231, Percent_Identity=28.1385281385281, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082096, Length=146, Percent_Identity=28.7671232876712, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081743, Length=235, Percent_Identity=30.2127659574468, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081845, Length=127, Percent_Identity=31.496062992126, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789650, Length=121, Percent_Identity=26.4462809917355, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788502, Length=222, Percent_Identity=27.9279279279279, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081980, Length=118, Percent_Identity=33.8983050847458, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 45303; Mature: 45303

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50925 BLUF ; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTIIYRSHICDNVSFKSIEAMVARANERNGQADVTGILLFNGTHFFQLIEGPEEKVQD
CCEEEEEHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEECCCHHHHHHH
IYQHICQDPRHYNLVELLCDYAPSRRFGKVGMELFDLREHDREEVLQAVMDRGTSKYQLT
HHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEE
YDDRALQFFRTFVEATEKANYFEIPSADSWVFIPDKETFYPDTPIIDNTEGCSFAFQPIV
EHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEHHHCC
DPFACEIISWEALLRTPDGQSPGAYFAGLTGDDIYLADLHSKRVALSLAGKLGLRNKALS
CHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEECCCCCEEEEHHHCCCCCCCEEE
INLLPMTMVKAPNAVAFLLDEISRNDLIPEQIIVEFTEREVISRMDDFTDAVRKLKGAGI
EEEEEEHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NLAIDHFGAGFAGLSLLAQYQPDRIKIDHELIRNIHQDGPRQSIVQAIIKCCTSLEIAVS
EEEEECCCCCHHHHHHHHHCCCCCEEECHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHH
AVGVERAEEWMWLESAGISQFQGNLFASARLGGLPAVAWPEKK
HHHHHHHHHHHHHHHCCCHHHCCCEEEEHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLTTIIYRSHICDNVSFKSIEAMVARANERNGQADVTGILLFNGTHFFQLIEGPEEKVQD
CCEEEEEHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEECCCHHHHHHH
IYQHICQDPRHYNLVELLCDYAPSRRFGKVGMELFDLREHDREEVLQAVMDRGTSKYQLT
HHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEE
YDDRALQFFRTFVEATEKANYFEIPSADSWVFIPDKETFYPDTPIIDNTEGCSFAFQPIV
EHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEHHHCC
DPFACEIISWEALLRTPDGQSPGAYFAGLTGDDIYLADLHSKRVALSLAGKLGLRNKALS
CHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEECCCCCEEEEHHHCCCCCCCEEE
INLLPMTMVKAPNAVAFLLDEISRNDLIPEQIIVEFTEREVISRMDDFTDAVRKLKGAGI
EEEEEEHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NLAIDHFGAGFAGLSLLAQYQPDRIKIDHELIRNIHQDGPRQSIVQAIIKCCTSLEIAVS
EEEEECCCCCHHHHHHHHHCCCCCEEECHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHH
AVGVERAEEWMWLESAGISQFQGNLFASARLGGLPAVAWPEKK
HHHHHHHHHHHHHHHCCCHHHCCCEEEEHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]