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The map label for this gene is yoaD [H]

Identifier: 152969207

GI number: 152969207

Start: 722499

End: 723527

Strand: Direct

Name: yoaD [H]

Synonym: KPN_00636

Alternate gene names: 152969207

Gene position: 722499-723527 (Clockwise)

Preceding gene: 152969206

Following gene: 152969213

Centisome position: 13.59

GC content: 64.04

Gene sequence:

>1029_bases
ATGAAAATCGTTTTTACCGCCGAGCATGCTGGCGACCTTAGCGCCTTCCACCAGCTGGGCGAGCTGCTGGTGGAGGGGTG
GGCGCTGGGCAAACCGAAACTCAGCGCCGGTCAGCTGATTGAGCTGGCCCACGACGCCGAGGTGCTGGTGACCAGCTATG
ATGAGGTGACGGACGCGGTGATGGCCGCCTGTCCGCGCCTGCAGGTAATCGCCTGCACCCGCGCGAATCCGGTGAATATT
GATACCCAGGCCGCCCAGGCGCGCGGCATCCGGGTGCTCTACACCCCGGGGCGCAATGCCGATGCCGCCGCGGAGCTGAC
GCTTGGCCTGATGCTCAGCCTGGCGCGCCATATTCCGCAATCCCATGCGGCGCTGAAGCGGGGGGAATTCACGCAGGCTG
ACAATGCCTCCGCCGCCACTCAGCAGGGTCTGCGGCGGGATGTGGTCTGGGACGTGAGCCCGGAAAGCCCCTATGAAGTG
TTTAAAGGCAGTGAACTGCGCAATAAAACGCTGGGGCTGGTGGGGTATGGCAACATTGGCCGCCGGGTGGCGCGTATCGC
CCGGGCGTTCGGCATGGCGGTGTTGGTGGTGGATCCGTTCGTCGCCGCAGAGGATATCAATGAGCCAGGGTTACAGAAAA
CGACCCTGGAGGCGCTGTTCCGCGAGGCGGATATCGTCAGCCTGCACCTGAGCAGCGGGCCGCACAGCGACGGGCTGGTG
AATGCCGCGCTGTTAAACAGCATGAAGCCCGGCGCGCTGCTGATCAATACCTCTCGCGCGGCGGTGGTCGACGAGGCAGC
GCTGATTGATGCCCTGCGCCACGGCCCGCTGGGCGGCGCGGCGCTGGATGTCTATCACCGCGAACCGCTGTGGCGCGATC
ATCCTTTTGTCATCGAGTTCGATAACGTGATTATCACGCCGCATATCGCCGGAGCCACCCGGGAGAGTATCGCCAAACAC
ACGGCGATGATTGCCGCCGATCTTCAGCGCTATGTCGCCGGGGAGCCGCTGCTGTACCAGTGGCGTTAA

Upstream 100 bases:

>100_bases
CGGCGCTGTATCCCGTTTACCGCCTGCTGCGCGAGCAGATGCTGCCGGTATGGCAGGCCAGACAGGCCGCCCTTCAACCT
CTTTCTCAGGACCTACAACC

Downstream 100 bases:

>100_bases
GCCGCTTGCGCCGCCGCGTTCTGCAGCGGCGCGGGTCCATCGTCAGGGGCGATGGGCCGCCATAAAAGCCTGGGTCTGTT
CCCAGTCCACTACCCCGGCA

Product: putative D-3-phosphoglycerate dehydrogenase

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKIVFTAEHAGDLSAFHQLGELLVEGWALGKPKLSAGQLIELAHDAEVLVTSYDEVTDAVMAACPRLQVIACTRANPVNI
DTQAAQARGIRVLYTPGRNADAAAELTLGLMLSLARHIPQSHAALKRGEFTQADNASAATQQGLRRDVVWDVSPESPYEV
FKGSELRNKTLGLVGYGNIGRRVARIARAFGMAVLVVDPFVAAEDINEPGLQKTTLEALFREADIVSLHLSSGPHSDGLV
NAALLNSMKPGALLINTSRAAVVDEAALIDALRHGPLGGAALDVYHREPLWRDHPFVIEFDNVIITPHIAGATRESIAKH
TAMIAADLQRYVAGEPLLYQWR

Sequences:

>Translated_342_residues
MKIVFTAEHAGDLSAFHQLGELLVEGWALGKPKLSAGQLIELAHDAEVLVTSYDEVTDAVMAACPRLQVIACTRANPVNI
DTQAAQARGIRVLYTPGRNADAAAELTLGLMLSLARHIPQSHAALKRGEFTQADNASAATQQGLRRDVVWDVSPESPYEV
FKGSELRNKTLGLVGYGNIGRRVARIARAFGMAVLVVDPFVAAEDINEPGLQKTTLEALFREADIVSLHLSSGPHSDGLV
NAALLNSMKPGALLINTSRAAVVDEAALIDALRHGPLGGAALDVYHREPLWRDHPFVIEFDNVIITPHIAGATRESIAKH
TAMIAADLQRYVAGEPLLYQWR
>Mature_342_residues
MKIVFTAEHAGDLSAFHQLGELLVEGWALGKPKLSAGQLIELAHDAEVLVTSYDEVTDAVMAACPRLQVIACTRANPVNI
DTQAAQARGIRVLYTPGRNADAAAELTLGLMLSLARHIPQSHAALKRGEFTQADNASAATQQGLRRDVVWDVSPESPYEV
FKGSELRNKTLGLVGYGNIGRRVARIARAFGMAVLVVDPFVAAEDINEPGLQKTTLEALFREADIVSLHLSSGPHSDGLV
NAALLNSMKPGALLINTSRAAVVDEAALIDALRHGPLGGAALDVYHREPLWRDHPFVIEFDNVIITPHIAGATRESIAKH
TAMIAADLQRYVAGEPLLYQWR

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=308, Percent_Identity=35.0649350649351, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI61743967, Length=258, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI4557497, Length=258, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI145580578, Length=258, Percent_Identity=32.1705426356589, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4557499, Length=258, Percent_Identity=32.1705426356589, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI145580575, Length=258, Percent_Identity=32.1705426356589, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI6912396, Length=277, Percent_Identity=29.2418772563177, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789279, Length=310, Percent_Identity=33.8709677419355, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787645, Length=284, Percent_Identity=31.3380281690141, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI87082289, Length=273, Percent_Identity=31.1355311355311, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788660, Length=339, Percent_Identity=28.023598820059, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081824, Length=169, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=299, Percent_Identity=32.7759197324415, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI25147481, Length=261, Percent_Identity=30.2681992337165, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322116, Length=316, Percent_Identity=30.379746835443, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320925, Length=316, Percent_Identity=30.6962025316456, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6324055, Length=301, Percent_Identity=25.9136212624585, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=314, Percent_Identity=25.796178343949, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=189, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921140, Length=270, Percent_Identity=32.5925925925926, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24646446, Length=271, Percent_Identity=32.8413284132841, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24646448, Length=271, Percent_Identity=32.8413284132841, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24646452, Length=271, Percent_Identity=32.8413284132841, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24646450, Length=271, Percent_Identity=32.8413284132841, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI62472511, Length=272, Percent_Identity=32.7205882352941, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI28574286, Length=279, Percent_Identity=28.673835125448, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45552429, Length=284, Percent_Identity=26.7605633802817, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585514, Length=284, Percent_Identity=26.7605633802817, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28574282, Length=284, Percent_Identity=26.7605633802817, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28574284, Length=284, Percent_Identity=26.7605633802817, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45551003, Length=284, Percent_Identity=26.7605633802817, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28571528, Length=287, Percent_Identity=28.9198606271777, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585516, Length=277, Percent_Identity=22.7436823104693, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95

Molecular weight: Translated: 36877; Mature: 36877

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVFTAEHAGDLSAFHQLGELLVEGWALGKPKLSAGQLIELAHDAEVLVTSYDEVTDAV
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHCCCEEEEECHHHHHHHH
MAACPRLQVIACTRANPVNIDTQAAQARGIRVLYTPGRNADAAAELTLGLMLSLARHIPQ
HHHCCCEEEEEECCCCCCCCCHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCH
SHAALKRGEFTQADNASAATQQGLRRDVVWDVSPESPYEVFKGSELRNKTLGLVGYGNIG
HHHHHHCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCHHHHCCCCCCCCEEEEEEECHHH
RRVARIARAFGMAVLVVDPFVAAEDINEPGLQKTTLEALFREADIVSLHLSSGPHSDGLV
HHHHHHHHHHCEEEEEECCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHH
NAALLNSMKPGALLINTSRAAVVDEAALIDALRHGPLGGAALDVYHREPLWRDHPFVIEF
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEE
DNVIITPHIAGATRESIAKHTAMIAADLQRYVAGEPLLYQWR
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MKIVFTAEHAGDLSAFHQLGELLVEGWALGKPKLSAGQLIELAHDAEVLVTSYDEVTDAV
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHCCCEEEEECHHHHHHHH
MAACPRLQVIACTRANPVNIDTQAAQARGIRVLYTPGRNADAAAELTLGLMLSLARHIPQ
HHHCCCEEEEEECCCCCCCCCHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCH
SHAALKRGEFTQADNASAATQQGLRRDVVWDVSPESPYEVFKGSELRNKTLGLVGYGNIG
HHHHHHCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCHHHHCCCCCCCCEEEEEEECHHH
RRVARIARAFGMAVLVVDPFVAAEDINEPGLQKTTLEALFREADIVSLHLSSGPHSDGLV
HHHHHHHHHHCEEEEEECCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHH
NAALLNSMKPGALLINTSRAAVVDEAALIDALRHGPLGGAALDVYHREPLWRDHPFVIEF
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEE
DNVIITPHIAGATRESIAKHTAMIAADLQRYVAGEPLLYQWR
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]