Click here to switch to the map view.

The map label for this gene is cof [H]

Identifier: 152968981

GI number: 152968981

Start: 451251

End: 452069

Strand: Direct

Name: cof [H]

Synonym: KPN_00408

Alternate gene names: 152968981

Gene position: 451251-452069 (Clockwise)

Preceding gene: 152968978

Following gene: 152968983

Centisome position: 8.49

GC content: 57.75

Gene sequence:

>819_bases
ATGGCAAAACTGGCGGCATTTGATATGGACGGCACCCTGCTGATGCCGGACCATCGTCTTGGAGAAAAGACGCTTTCCGC
GCTGAAACGGCTGCGTGAGCGCGATATCACCTTGACCTTCGCCACCGGCCGCCATGTGCTGGAGATGCACCATGTGATTG
GCGAATTTTCCCTTGATGCATTTCTGGTCACCGGCAACGGGACGCGCATCCATTCGCTGGAAGGGGAGGAGCTTTATCGC
CAGGATCTCGCTCCCGAAGCGGCGGAAGCGGTGCTGCACGGCAAATGGGATACCCAGGCCAGCATGCACGTCTTTAACGA
TGGCGGCTGGTTCACCGGCCAGGCAAGGCCTGAATTGTTGCAAGCGCATGTATTCAGCGGCTTTCACTATCAACTGTGCG
ATCTGAAGCGTATGTCGGCGCAGCATGTCACCAAGATCTGTTTCTGCGGCGATCATGACGATTTGCGCCGTCTGCGGATC
CAGCTGAATGAGACGCTGGGCGATCGCGCATTTCTCTGCTTCTCGGCAATGGACTGTCTGGAGGTGCTGCCGGTTGGCTG
CAATAAAGGCGCGGCGCTGGCGGTGCTCAGCCAGCATTTAGGCTTCACCCTGCAGGAGTGCATGGCCTTCGGCGATGCGA
TGAACGACCGCGAGATGCTGGGCAGCGTTGGGCGGGGGTTCATCATGGGCAACGCGATGCCGCAGCTGAAAGCCGAGCTG
CCGCATTTGCCGGTAATTGGCGACTGCCGGAATCAGGCAGTCTCCCATTTTTTAACTCATTGGCTGGATAATCCTGATCT
TCCTTATTCCCCCGAATAG

Upstream 100 bases:

>100_bases
AAACCCGGAACAATTAATAATATCTATTCACTATTACTTCCGTATATCCCAGGTGATACTGCAACACAAGCGAAGCCCAT
CACACATCGGGGAATATACC

Downstream 100 bases:

>100_bases
AGAGACCCTTCCAGCAAGCCAGATAGGCTTCTATCTGGCTTTTTTTATCGCCTTTCGACCAGACCGGCGATCTCCGCCTG
CCAGGGCGCAATATCGCCGA

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MAKLAAFDMDGTLLMPDHRLGEKTLSALKRLRERDITLTFATGRHVLEMHHVIGEFSLDAFLVTGNGTRIHSLEGEELYR
QDLAPEAAEAVLHGKWDTQASMHVFNDGGWFTGQARPELLQAHVFSGFHYQLCDLKRMSAQHVTKICFCGDHDDLRRLRI
QLNETLGDRAFLCFSAMDCLEVLPVGCNKGAALAVLSQHLGFTLQECMAFGDAMNDREMLGSVGRGFIMGNAMPQLKAEL
PHLPVIGDCRNQAVSHFLTHWLDNPDLPYSPE

Sequences:

>Translated_272_residues
MAKLAAFDMDGTLLMPDHRLGEKTLSALKRLRERDITLTFATGRHVLEMHHVIGEFSLDAFLVTGNGTRIHSLEGEELYR
QDLAPEAAEAVLHGKWDTQASMHVFNDGGWFTGQARPELLQAHVFSGFHYQLCDLKRMSAQHVTKICFCGDHDDLRRLRI
QLNETLGDRAFLCFSAMDCLEVLPVGCNKGAALAVLSQHLGFTLQECMAFGDAMNDREMLGSVGRGFIMGNAMPQLKAEL
PHLPVIGDCRNQAVSHFLTHWLDNPDLPYSPE
>Mature_271_residues
AKLAAFDMDGTLLMPDHRLGEKTLSALKRLRERDITLTFATGRHVLEMHHVIGEFSLDAFLVTGNGTRIHSLEGEELYRQ
DLAPEAAEAVLHGKWDTQASMHVFNDGGWFTGQARPELLQAHVFSGFHYQLCDLKRMSAQHVTKICFCGDHDDLRRLRIQ
LNETLGDRAFLCFSAMDCLEVLPVGCNKGAALAVLSQHLGFTLQECMAFGDAMNDREMLGSVGRGFIMGNAMPQLKAELP
HLPVIGDCRNQAVSHFLTHWLDNPDLPYSPE

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081741, Length=272, Percent_Identity=77.2058823529412, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI48994981, Length=260, Percent_Identity=36.9230769230769, Blast_Score=175, Evalue=2e-45,
Organism=Escherichia coli, GI1786982, Length=277, Percent_Identity=27.0758122743682, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI2367265, Length=242, Percent_Identity=24.3801652892562, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 30359; Mature: 30228

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLAAFDMDGTLLMPDHRLGEKTLSALKRLRERDITLTFATGRHVLEMHHVIGEFSLDA
CCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCEE
FLVTGNGTRIHSLEGEELYRQDLAPEAAEAVLHGKWDTQASMHVFNDGGWFTGQARPELL
EEEECCCCEEECCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCHHHH
QAHVFSGFHYQLCDLKRMSAQHVTKICFCGDHDDLRRLRIQLNETLGDRAFLCFSAMDCL
HHHHHCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EVLPVGCNKGAALAVLSQHLGFTLQECMAFGDAMNDREMLGSVGRGFIMGNAMPQLKAEL
HHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCHHHHHCC
PHLPVIGDCRNQAVSHFLTHWLDNPDLPYSPE
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AKLAAFDMDGTLLMPDHRLGEKTLSALKRLRERDITLTFATGRHVLEMHHVIGEFSLDA
CCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCEE
FLVTGNGTRIHSLEGEELYRQDLAPEAAEAVLHGKWDTQASMHVFNDGGWFTGQARPELL
EEEECCCCEEECCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCHHHH
QAHVFSGFHYQLCDLKRMSAQHVTKICFCGDHDDLRRLRIQLNETLGDRAFLCFSAMDCL
HHHHHCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EVLPVGCNKGAALAVLSQHLGFTLQECMAFGDAMNDREMLGSVGRGFIMGNAMPQLKAEL
HHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCHHHHHCC
PHLPVIGDCRNQAVSHFLTHWLDNPDLPYSPE
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA