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The map label for this gene is leuO [H]

Identifier: 152968662

GI number: 152968662

Start: 96049

End: 96933

Strand: Direct

Name: leuO [H]

Synonym: KPN_00081

Alternate gene names: 152968662

Gene position: 96049-96933 (Clockwise)

Preceding gene: 152968653

Following gene: 152968663

Centisome position: 1.81

GC content: 51.75

Gene sequence:

>885_bases
ATGGTTGATCTCAATCTGCTTACCGTTTTTGATGCGGTTATGCAAGAGCAGAATATTACTCGTGCCGCCCATACGCTCGG
CATGTCACAACCCGCGGTGAGCAATGCGGTTTCACGACTGAAAGTGATGTTTAATGACGAGCTTTTTGTGCGCTATGGCC
GCGGTATTCAGCCGACGGCGCGCGCCTATCAGCTGTTTGGCTCGGTACGCCAGGCGCTGCAGCTGGTCCAAAACGAATTG
CCAGGCTCCGGTTTTGAACCGCTGAGCAGTGAGCGCGTGTTCCATCTGTGCGTCTGCAGCCCGCTGGATAACATCCTGAC
GTCGGTTATTTTTAATAAAGTTGCAGAGATAGCACCCAATATTCATTTAGTCTTTAAGTCTGCGCTGAATCAAAATACGG
AACATCAGCTACGTTATCAGGAAACAGAATTCGTAATCGGTTATGAAGAGTTCCGTCGCCCTGAGTTTGCCTGCGTTCCG
TTATTTAAAGACGAAATGGTCCTGGTCGCCAGTCGCAACCATCCGCGTATTTCCGGGCCGATGATGGAGGCGGATATTTA
TCGCGAGGAGCATGCCGCCGTTGCTCTCGACCGGTATGCCTCATTTAGTTTGCCGTGGTATGACACGGCCGATAAACAAG
CGCGTATCGCCTATCAGGGTAATGCGATGGTCAGCGTATTAAACGTAGTATCGCAAACCCAGATGGTGGCCATTGCCCCG
CGCTGGCTGGCAGATGAATTTGCTGACAAGCTTGCCCTGCAAATATTACCTTTGCCGTTGAAGGTCAACAGTCGGACCTG
CTATCTCTCCTGGCACGAAGCCGCTGGTCGTGATAAAGGCCACCAGTGGATGGAAGAGCTGTTGGTGGACATTTGCAAGC
GCTGA

Upstream 100 bases:

>100_bases
GTGAAGGGAATTCATCGTGACGGTGGAATTAGATATGTTAATTGAAACTCCAGAATATAAATCAGCCCCCGGACAGGAAG
TCGTTAAACCTCAACTACGG

Downstream 100 bases:

>100_bases
CCCAACGACGATAGATAGCAAAGGAGCTTCGGCTCCTTTTTTTATATGACATAAATCAAATAAAGCAGAATTTTATTCTG
TTTACCTTGCGCGAGGGGTT

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVSRLKVMFNDELFVRYGRGIQPTARAYQLFGSVRQALQLVQNEL
PGSGFEPLSSERVFHLCVCSPLDNILTSVIFNKVAEIAPNIHLVFKSALNQNTEHQLRYQETEFVIGYEEFRRPEFACVP
LFKDEMVLVASRNHPRISGPMMEADIYREEHAAVALDRYASFSLPWYDTADKQARIAYQGNAMVSVLNVVSQTQMVAIAP
RWLADEFADKLALQILPLPLKVNSRTCYLSWHEAAGRDKGHQWMEELLVDICKR

Sequences:

>Translated_294_residues
MVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVSRLKVMFNDELFVRYGRGIQPTARAYQLFGSVRQALQLVQNEL
PGSGFEPLSSERVFHLCVCSPLDNILTSVIFNKVAEIAPNIHLVFKSALNQNTEHQLRYQETEFVIGYEEFRRPEFACVP
LFKDEMVLVASRNHPRISGPMMEADIYREEHAAVALDRYASFSLPWYDTADKQARIAYQGNAMVSVLNVVSQTQMVAIAP
RWLADEFADKLALQILPLPLKVNSRTCYLSWHEAAGRDKGHQWMEELLVDICKR
>Mature_294_residues
MVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVSRLKVMFNDELFVRYGRGIQPTARAYQLFGSVRQALQLVQNEL
PGSGFEPLSSERVFHLCVCSPLDNILTSVIFNKVAEIAPNIHLVFKSALNQNTEHQLRYQETEFVIGYEEFRRPEFACVP
LFKDEMVLVASRNHPRISGPMMEADIYREEHAAVALDRYASFSLPWYDTADKQARIAYQGNAMVSVLNVVSQTQMVAIAP
RWLADEFADKLALQILPLPLKVNSRTCYLSWHEAAGRDKGHQWMEELLVDICKR

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=294, Percent_Identity=84.0136054421769, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1786820, Length=278, Percent_Identity=24.8201438848921, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081766, Length=275, Percent_Identity=26.1818181818182, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33376; Mature: 33376

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVSRLKVMFNDELFVRYGRGIQPTA
CCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHH
RAYQLFGSVRQALQLVQNELPGSGFEPLSSERVFHLCVCSPLDNILTSVIFNKVAEIAPN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCC
IHLVFKSALNQNTEHQLRYQETEFVIGYEEFRRPEFACVPLFKDEMVLVASRNHPRISGP
HHEEEHHHHCCCCHHHHEEECCEEEEEHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCC
MMEADIYREEHAAVALDRYASFSLPWYDTADKQARIAYQGNAMVSVLNVVSQTQMVAIAP
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHEEEECC
RWLADEFADKLALQILPLPLKVNSRTCYLSWHEAAGRDKGHQWMEELLVDICKR
HHHHHHHHHHHHHHHCCCCEEECCEEEEEEEHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVSRLKVMFNDELFVRYGRGIQPTA
CCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHH
RAYQLFGSVRQALQLVQNELPGSGFEPLSSERVFHLCVCSPLDNILTSVIFNKVAEIAPN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCC
IHLVFKSALNQNTEHQLRYQETEFVIGYEEFRRPEFACVPLFKDEMVLVASRNHPRISGP
HHEEEHHHHCCCCHHHHEEECCEEEEEHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCC
MMEADIYREEHAAVALDRYASFSLPWYDTADKQARIAYQGNAMVSVLNVVSQTQMVAIAP
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHEEEECC
RWLADEFADKLALQILPLPLKVNSRTCYLSWHEAAGRDKGHQWMEELLVDICKR
HHHHHHHHHHHHHHHCCCCEEECCEEEEEEEHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]