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The map label for this gene is aksF [H]

Identifier: 150401906

GI number: 150401906

Start: 1545336

End: 1546367

Strand: Reverse

Name: aksF [H]

Synonym: Maeo_1484

Alternate gene names: 150401906

Gene position: 1546367-1545336 (Counterclockwise)

Preceding gene: 150401907

Following gene: 150401905

Centisome position: 98.53

GC content: 33.24

Gene sequence:

>1032_bases
TTGAAAATACCTAAAATATGTGTGATAGAAGGGGACGGCATAGGAAAAGAAGTAATACCTGAAACAGTCCGCATATTAAA
AGAAATAGGGGACTTTGAATTCATATATGAACATGCGGGATATGAATGTTTTAAACGGTGCGGTGATGCAATACCCGAAA
AAACATTAAAAACTGCAAAAGAATGCGATGCCATATTATTTGGTGCAGTTAGCACCCCTAAATTAGATGAAACGGAGCGA
AAACCATATAAAAGCCCCATATTAACACTTAGAAAAGAATTAGACCTATATGCAAATGTAAGACCAATACATAAATTAGA
TAATTCCGATAGTTCAAATAATATAGATTTCATAATAATTCGAGAAAACACAGAAGGACTATATTCTGGTGTGGAATATT
ATGACGAAGAAAAAGAGTTGGCAATATCGGAAAGGCATATATCTAAAAAAGGAAGCAAAAGAATTATTAAATTTGCATTT
GAATATGCTGTGAAACATCACAGAAAAAAAGTATCCTGTATCCATAAATCAAATATTTTAAGAATTACGGACGGTCTTTT
TTTAAACATATTCAATGAATTTAAAGAAAAATATAAAAATGAATACAATATAGAAGGAAACGATTATTTAGTAGATGCTA
CGGCAATGTATATTTTAAAAAGCCCACAAATGTTCGATGTAATTGTAACTACAAATTTATTTGGTGACATATTATCCGAT
GAAGCATCGGGATTATTGGGAGGTTTAGGCTTGGCTCCTTCAGCAAATATTGGGGATAATTATGGATTATTTGAGCCTGT
GCATGGTTCCGCCCCAGACATAGCTGGAAAAGGTGTTGCAAATCCGATAGCTGCGGTTCTTAGTGCTTCGATGATGCTAT
ATTACCTAGATATGAAGGAGAAAAGTAGGCTATTAAAAGATGCCGTAAAACAGGTGTTGGCCCATAAAGATATAACTCCT
GATTTAGGGGGCAATTTAAAAACAAAGGAAGTAAGCGATAAAATTATTGAAGAATTACGGAAAATATCCTAA

Upstream 100 bases:

>100_bases
TATTATTCTATTTTTTTAGCATACATTTATATTTTATAAACCCTTATTTTTTAGTTAATTTATGATTTATCTCATCATTA
TATATTATCGCGGTGATATT

Downstream 100 bases:

>100_bases
AATTAAATAATAAGAATAATAAGAATAAAAATATTAATGATACAATAATAAAAAATACGATAATAAATATGTTAAATTAT
AGTAAGTTCAAAAACTGTCC

Product: isopropylmalate/isohomocitrate dehydrogenase

Products: NA

Alternate protein names: HICDH; Isohomocitrate dehydrogenase; IHDH; NAD-dependent threo-isohomocitrate dehydrogenase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKIPKICVIEGDGIGKEVIPETVRILKEIGDFEFIYEHAGYECFKRCGDAIPEKTLKTAKECDAILFGAVSTPKLDETER
KPYKSPILTLRKELDLYANVRPIHKLDNSDSSNNIDFIIIRENTEGLYSGVEYYDEEKELAISERHISKKGSKRIIKFAF
EYAVKHHRKKVSCIHKSNILRITDGLFLNIFNEFKEKYKNEYNIEGNDYLVDATAMYILKSPQMFDVIVTTNLFGDILSD
EASGLLGGLGLAPSANIGDNYGLFEPVHGSAPDIAGKGVANPIAAVLSASMMLYYLDMKEKSRLLKDAVKQVLAHKDITP
DLGGNLKTKEVSDKIIEELRKIS

Sequences:

>Translated_343_residues
MKIPKICVIEGDGIGKEVIPETVRILKEIGDFEFIYEHAGYECFKRCGDAIPEKTLKTAKECDAILFGAVSTPKLDETER
KPYKSPILTLRKELDLYANVRPIHKLDNSDSSNNIDFIIIRENTEGLYSGVEYYDEEKELAISERHISKKGSKRIIKFAF
EYAVKHHRKKVSCIHKSNILRITDGLFLNIFNEFKEKYKNEYNIEGNDYLVDATAMYILKSPQMFDVIVTTNLFGDILSD
EASGLLGGLGLAPSANIGDNYGLFEPVHGSAPDIAGKGVANPIAAVLSASMMLYYLDMKEKSRLLKDAVKQVLAHKDITP
DLGGNLKTKEVSDKIIEELRKIS
>Mature_343_residues
MKIPKICVIEGDGIGKEVIPETVRILKEIGDFEFIYEHAGYECFKRCGDAIPEKTLKTAKECDAILFGAVSTPKLDETER
KPYKSPILTLRKELDLYANVRPIHKLDNSDSSNNIDFIIIRENTEGLYSGVEYYDEEKELAISERHISKKGSKRIIKFAF
EYAVKHHRKKVSCIHKSNILRITDGLFLNIFNEFKEKYKNEYNIEGNDYLVDATAMYILKSPQMFDVIVTTNLFGDILSD
EASGLLGGLGLAPSANIGDNYGLFEPVHGSAPDIAGKGVANPIAAVLSASMMLYYLDMKEKSRLLKDAVKQVLAHKDITP
DLGGNLKTKEVSDKIIEELRKIS

Specific function: Catalyzes the NAD-dependent oxidation and decarboxylation of (2R,3S)-homoisocitrate ((1R,2S)-1- hydroxybutane-1,2,4-tricarboxylate), (2R,3S)-homo(2)-isocitrate and (2R,3S)-homo(3)-isocitrate, into 2-oxoadipate, 2-oxopimelate, and 2-oxosuberate, respective

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=345, Percent_Identity=37.1014492753623, Blast_Score=211, Evalue=6e-55,
Organism=Homo sapiens, GI28178816, Length=338, Percent_Identity=33.4319526627219, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI28178821, Length=339, Percent_Identity=33.6283185840708, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=33.5260115606936, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI28178838, Length=313, Percent_Identity=33.2268370607029, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI28178819, Length=197, Percent_Identity=37.5634517766497, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=36.7688022284123, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI87081683, Length=354, Percent_Identity=33.6158192090396, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787381, Length=396, Percent_Identity=30.5555555555556, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71986051, Length=327, Percent_Identity=38.8379204892966, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI25144293, Length=346, Percent_Identity=34.971098265896, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17550882, Length=344, Percent_Identity=34.3023255813954, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17505779, Length=341, Percent_Identity=32.8445747800587, Blast_Score=174, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=42.2857142857143, Blast_Score=260, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=35.5371900826446, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6324709, Length=341, Percent_Identity=36.6568914956012, Blast_Score=195, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6324291, Length=340, Percent_Identity=35.2941176470588, Blast_Score=182, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24661184, Length=342, Percent_Identity=36.5497076023392, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24643270, Length=339, Percent_Identity=37.4631268436578, Blast_Score=215, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24643268, Length=339, Percent_Identity=37.4631268436578, Blast_Score=214, Evalue=5e-56,
Organism=Drosophila melanogaster, GI161078633, Length=340, Percent_Identity=32.9411764705882, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24650122, Length=340, Percent_Identity=32.9411764705882, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI161078637, Length=340, Percent_Identity=32.9411764705882, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI161078635, Length=340, Percent_Identity=32.9411764705882, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI161078639, Length=338, Percent_Identity=33.1360946745562, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI281362242, Length=339, Percent_Identity=34.8082595870207, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24648872, Length=339, Percent_Identity=34.8082595870207, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI20130355, Length=344, Percent_Identity=27.6162790697674, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45550436, Length=232, Percent_Identity=23.7068965517241, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.87 [H]

Molecular weight: Translated: 38476; Mature: 38476

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPKICVIEGDGIGKEVIPETVRILKEIGDFEFIYEHAGYECFKRCGDAIPEKTLKTAK
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
ECDAILFGAVSTPKLDETERKPYKSPILTLRKELDLYANVRPIHKLDNSDSSNNIDFIII
HCCCEEEECCCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEE
RENTEGLYSGVEYYDEEKELAISERHISKKGSKRIIKFAFEYAVKHHRKKVSCIHKSNIL
ECCCCHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
RITDGLFLNIFNEFKEKYKNEYNIEGNDYLVDATAMYILKSPQMFDVIVTTNLFGDILSD
EECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHEEHHCCCCCEEEEEHHHHHHHHHHH
EASGLLGGLGLAPSANIGDNYGLFEPVHGSAPDIAGKGVANPIAAVLSASMMLYYLDMKE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHH
KSRLLKDAVKQVLAHKDITPDLGGNLKTKEVSDKIIEELRKIS
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIPKICVIEGDGIGKEVIPETVRILKEIGDFEFIYEHAGYECFKRCGDAIPEKTLKTAK
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
ECDAILFGAVSTPKLDETERKPYKSPILTLRKELDLYANVRPIHKLDNSDSSNNIDFIII
HCCCEEEECCCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEE
RENTEGLYSGVEYYDEEKELAISERHISKKGSKRIIKFAFEYAVKHHRKKVSCIHKSNIL
ECCCCHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
RITDGLFLNIFNEFKEKYKNEYNIEGNDYLVDATAMYILKSPQMFDVIVTTNLFGDILSD
EECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHEEHHCCCCCEEEEEHHHHHHHHHHH
EASGLLGGLGLAPSANIGDNYGLFEPVHGSAPDIAGKGVANPIAAVLSASMMLYYLDMKE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHH
KSRLLKDAVKQVLAHKDITPDLGGNLKTKEVSDKIIEELRKIS
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051 [H]