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The map label for this gene is pdxT

Identifier: 150401861

GI number: 150401861

Start: 1496502

End: 1497062

Strand: Direct

Name: pdxT

Synonym: Maeo_1439

Alternate gene names: 150401861

Gene position: 1496502-1497062 (Clockwise)

Preceding gene: 150401859

Following gene: 150401862

Centisome position: 95.35

GC content: 32.8

Gene sequence:

>561_bases
TTGAATATAGGAATATTAGGAATTCAGGGAGATATTGAAGAACATGAGGAAATGATTAAAAAAATAAACCATGCCCCAAA
AAGAATACGAACAATAGAGGATTTAAAAAATATTGATGCTCTTATTATCCCCGGAGGAGAGAGCTCCACAATGGGCAAAT
TGATGAAAACCTATGGATTTATTGAAGCTTTAAAAAATGCCGATTTGCCCATATTGGGAACTTGTGCGGGTATGGTGCTA
TTATCAAAAGGAACTGGAAAAGAACAACCACTATTGGAGTTAATGGATATTACAATAAATAGAAATGCCTATGGCAGTCA
AAAATATAGTTTTGAATCAGAATTGGAGTTAAATGGCATAAAAATAAATGCAGTATTTATCAGAGCTCCCACAGTAGATA
AAATATTAAGCGATGAAGTGGAGATAATAGCAAAAGAAGGAGGCAATATTGTCGGTGTTAAACAGGGAAAATACATGGCA
ATAGCTTTCCATCCAGAACTATCAGAAGAAGGATATAAATTTTATGAATACTTTTTAAATGAAGTGGTAAAAAATGACTA
A

Upstream 100 bases:

>100_bases
AACCGTATGAGCTCTGAGACATATTATTTTTTTATTAATTATATTAATTATTTTTTTTATATGCCAATATTTTAATTTTA
TAATTTAAAAAGGTGAGAAT

Downstream 100 bases:

>100_bases
AATAATAGCAGTTAGTGGAAAAGGTGGCACAGGAAAAACAATGTTTTCTACTCTATTGGTAAAGGCAATATCTCAAAAAA
CAAATAATTTATTGGTAATT

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MNIGILGIQGDIEEHEEMIKKINHAPKRIRTIEDLKNIDALIIPGGESSTMGKLMKTYGFIEALKNADLPILGTCAGMVL
LSKGTGKEQPLLELMDITINRNAYGSQKYSFESELELNGIKINAVFIRAPTVDKILSDEVEIIAKEGGNIVGVKQGKYMA
IAFHPELSEEGYKFYEYFLNEVVKND

Sequences:

>Translated_186_residues
MNIGILGIQGDIEEHEEMIKKINHAPKRIRTIEDLKNIDALIIPGGESSTMGKLMKTYGFIEALKNADLPILGTCAGMVL
LSKGTGKEQPLLELMDITINRNAYGSQKYSFESELELNGIKINAVFIRAPTVDKILSDEVEIIAKEGGNIVGVKQGKYMA
IAFHPELSEEGYKFYEYFLNEVVKND
>Mature_186_residues
MNIGILGIQGDIEEHEEMIKKINHAPKRIRTIEDLKNIDALIIPGGESSTMGKLMKTYGFIEALKNADLPILGTCAGMVL
LSKGTGKEQPLLELMDITINRNAYGSQKYSFESELELNGIKINAVFIRAPTVDKILSDEVEIIAKEGGNIVGVKQGKYMA
IAFHPELSEEGYKFYEYFLNEVVKND

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=210, Percent_Identity=30.4761904761905, Blast_Score=107, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6323995, Length=212, Percent_Identity=33.0188679245283, Blast_Score=101, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321048, Length=212, Percent_Identity=32.5471698113208, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_META3 (A6UWZ3)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325627.1
- ProteinModelPortal:   A6UWZ3
- SMR:   A6UWZ3
- STRING:   A6UWZ3
- GeneID:   5327443
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1439
- eggNOG:   arNOG08412
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13527
- BioCyc:   MAEO419665:MAEO_1439-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 20666; Mature: 20666

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 75-75 ACT_SITE 164-164 ACT_SITE 166-166 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGILGIQGDIEEHEEMIKKINHAPKRIRTIEDLKNIDALIIPGGESSTMGKLMKTYGF
CCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHH
IEALKNADLPILGTCAGMVLLSKGTGKEQPLLELMDITINRNAYGSQKYSFESELELNGI
HHHHHCCCCCEEEHHHCEEEEECCCCCCCCHHHHHHEEECCCCCCCCCCCCCCCEEECCE
KINAVFIRAPTVDKILSDEVEIIAKEGGNIVGVKQGKYMAIAFHPELSEEGYKFYEYFLN
EEEEEEEECCCHHHHHHHHHEEHCCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHH
EVVKND
HHHCCC
>Mature Secondary Structure
MNIGILGIQGDIEEHEEMIKKINHAPKRIRTIEDLKNIDALIIPGGESSTMGKLMKTYGF
CCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHH
IEALKNADLPILGTCAGMVLLSKGTGKEQPLLELMDITINRNAYGSQKYSFESELELNGI
HHHHHCCCCCEEEHHHCEEEEECCCCCCCCHHHHHHEEECCCCCCCCCCCCCCCEEECCE
KINAVFIRAPTVDKILSDEVEIIAKEGGNIVGVKQGKYMAIAFHPELSEEGYKFYEYFLN
EEEEEEEECCCHHHHHHHHHEEHCCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHH
EVVKND
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA