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The map label for this gene is cofF [H]

Identifier: 150401801

GI number: 150401801

Start: 1443541

End: 1444440

Strand: Reverse

Name: cofF [H]

Synonym: Maeo_1379

Alternate gene names: 150401801

Gene position: 1444440-1443541 (Counterclockwise)

Preceding gene: 150401802

Following gene: 150401800

Centisome position: 92.03

GC content: 28.44

Gene sequence:

>900_bases
ATGTATGTTATTTTATTATTTTTCCCAAATAAAGGTGCTATTATGATTACAATAATTTGTGCAGAAGGCGGTTCCTCAAT
AAACAGCCTAAAAAAATCAATAGAAGAATTGGGAGAAAAATGCGAAATACTATTTTTATCAGACGATAATTTGCAGATAG
ACCAATCGTTTAATATAGATAGTGATATTATTCATTCCCGATGCGGTATTGGTGAATATCTTGATAAATTAACTCTTTTT
TCTTGGCAGATATTGAATGTCCTTGAAGTCGAAAACCATATTTTTATTAATTCATTGAAAACGATATATAATTCAGCGGA
TAAATTTAAAACCATTAAATTATTATCTAAAAATAATATAGAAACTCCAAAAACGGGATTAATAAGAGATTATAACGATG
CAAAATTGTTTATGTCAAAAAATAATATAAATTATCCTGTAATTATAAAAAATTGCTTTTCAAAATGTGGAAATGGAGTA
AAATTAATAAAAAATGATGCCGAATTAAAAGAAGCTTCAAAAAATGCCATGTGGTATGGAACTACAATTCAAGAATATGT
TGAATTTGGAAATAATGGAATATATAAAGATATTAGGATATTAGTTGTAGATGGTGAAGTTGTGGGAGGATATAGAAGAG
TGAGCTCCAATTTTATAACTAATCTACATCAAGGGAATAAGGTGGAGCTCCTAAAAATAAATGATGAATTATCAGAAATA
GCTCTAAAAAGTGCCGAAGCTATCGATGGGCAAATAGTTGGAGTGGACATATTGCCCTCAAAAGAGGGATATAAAGTATT
AGAAACCAACACAGCACCGGGAACCAAAGGATTTAGAGAATTGGGAATTAATGTAGATAAAAAAATAGCAAAATGTTTAA
TTAATTATAAAAAGATATGA

Upstream 100 bases:

>100_bases
TAAATGAAAGAAGCTTAACCTCAATTAGTTTAATGGTTTGGGGATTAATATTTATATCAATGGCATATTCATTTATGTAA
TATTGATATATATTATTTTT

Downstream 100 bases:

>100_bases
GTAATATACCATATAACAATATAAAAATAATAATGGAAAAATTAATAATGGGGGAGCTCCATGTTTGAAAATTTAATATA
TTCTTTTAGTTTTGTGCTGT

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MYVILLFFPNKGAIMITIICAEGGSSINSLKKSIEELGEKCEILFLSDDNLQIDQSFNIDSDIIHSRCGIGEYLDKLTLF
SWQILNVLEVENHIFINSLKTIYNSADKFKTIKLLSKNNIETPKTGLIRDYNDAKLFMSKNNINYPVIIKNCFSKCGNGV
KLIKNDAELKEASKNAMWYGTTIQEYVEFGNNGIYKDIRILVVDGEVVGGYRRVSSNFITNLHQGNKVELLKINDELSEI
ALKSAEAIDGQIVGVDILPSKEGYKVLETNTAPGTKGFRELGINVDKKIAKCLINYKKI

Sequences:

>Translated_299_residues
MYVILLFFPNKGAIMITIICAEGGSSINSLKKSIEELGEKCEILFLSDDNLQIDQSFNIDSDIIHSRCGIGEYLDKLTLF
SWQILNVLEVENHIFINSLKTIYNSADKFKTIKLLSKNNIETPKTGLIRDYNDAKLFMSKNNINYPVIIKNCFSKCGNGV
KLIKNDAELKEASKNAMWYGTTIQEYVEFGNNGIYKDIRILVVDGEVVGGYRRVSSNFITNLHQGNKVELLKINDELSEI
ALKSAEAIDGQIVGVDILPSKEGYKVLETNTAPGTKGFRELGINVDKKIAKCLINYKKI
>Mature_299_residues
MYVILLFFPNKGAIMITIICAEGGSSINSLKKSIEELGEKCEILFLSDDNLQIDQSFNIDSDIIHSRCGIGEYLDKLTLF
SWQILNVLEVENHIFINSLKTIYNSADKFKTIKLLSKNNIETPKTGLIRDYNDAKLFMSKNNINYPVIIKNCFSKCGNGV
KLIKNDAELKEASKNAMWYGTTIQEYVEFGNNGIYKDIRILVVDGEVVGGYRRVSSNFITNLHQGNKVELLKINDELSEI
ALKSAEAIDGQIVGVDILPSKEGYKVLETNTAPGTKGFRELGINVDKKIAKCLINYKKI

Specific function: Catalyzes the ATP-dependent addition of one L-glutamate molecule to gamma-F420-2, producing the mature coenzyme alpha- F420-3 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=283, Percent_Identity=26.5017667844523, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33536; Mature: 33536

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVILLFFPNKGAIMITIICAEGGSSINSLKKSIEELGEKCEILFLSDDNLQIDQSFNID
CEEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCEEEECCCCCC
SDIIHSRCGIGEYLDKLTLFSWQILNVLEVENHIFINSLKTIYNSADKFKTIKLLSKNNI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCCHHHHEEEEEEECCCC
ETPKTGLIRDYNDAKLFMSKNNINYPVIIKNCFSKCGNGVKLIKNDAELKEASKNAMWYG
CCCCCCCEECCCCCEEEEECCCCCCCEEHHHHHHHCCCCEEEEECCHHHHHHCCCCEEEC
TTIQEYVEFGNNGIYKDIRILVVDGEVVGGYRRVSSNFITNLHQGNKVELLKINDELSEI
CHHHHHHHHCCCCCEEEEEEEEECCCEECCHHHHHHHHHHHCCCCCEEEEEEECCHHHHH
ALKSAEAIDGQIVGVDILPSKEGYKVLETNTAPGTKGFRELGINVDKKIAKCLINYKKI
HHHHHHHCCCEEEEEEEECCCCCCEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYVILLFFPNKGAIMITIICAEGGSSINSLKKSIEELGEKCEILFLSDDNLQIDQSFNID
CEEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCEEEECCCCCC
SDIIHSRCGIGEYLDKLTLFSWQILNVLEVENHIFINSLKTIYNSADKFKTIKLLSKNNI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCCHHHHEEEEEEECCCC
ETPKTGLIRDYNDAKLFMSKNNINYPVIIKNCFSKCGNGVKLIKNDAELKEASKNAMWYG
CCCCCCCEECCCCCEEEEECCCCCCCEEHHHHHHHCCCCEEEEECCHHHHHHCCCCEEEC
TTIQEYVEFGNNGIYKDIRILVVDGEVVGGYRRVSSNFITNLHQGNKVELLKINDELSEI
CHHHHHHHHCCCCCEEEEEEEEECCCEECCHHHHHHHHHHHCCCCCEEEEEEECCHHHHH
ALKSAEAIDGQIVGVDILPSKEGYKVLETNTAPGTKGFRELGINVDKKIAKCLINYKKI
HHHHHHHCCCEEEEEEEECCCCCCEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA