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The map label for this gene is rfaE [C]

Identifier: 150401726

GI number: 150401726

Start: 1365618

End: 1366088

Strand: Reverse

Name: rfaE [C]

Synonym: Maeo_1304

Alternate gene names: 150401726

Gene position: 1366088-1365618 (Counterclockwise)

Preceding gene: 150401727

Following gene: 150401720

Centisome position: 87.04

GC content: 28.24

Gene sequence:

>471_bases
ATGAAAACCAACGATAAAACTAAAAAAATAGTGCTAACTGCGGGAACTTTTGATTTACTCCATCCTGGACATCACAATAC
TTTAAAATATGCCAAAAGTTTAGGAGATGAATTGATTGTAGTAATAGCAAGAGATGAAACCGTAAAAAAAATAAAAGGTA
GAAAACCGGTAATACCAGAGAATCAGAGAAGAGAAATGATAGAGGCGATTAAACCGGTTGATAAAGCTATATTGGGTAGT
TTAACTGATAAATTAGAACCTATTCTTAAAATAAAACCAGATATTATAGTTTTAGGTCCTGACCAAATTACTTTTGATGC
TGAAAATTTAAAAAACGAATTGAAAAAATTAGGATTAGATGTTGAAATTGTAAAAACTAAGGAATATACTAATTGTGATT
TCCATAGTTCCTACGATATAATAAAGGAAATTGTTAAAAGATGGTGCAATAAAAAAATAATAAAAAAATAG

Upstream 100 bases:

>100_bases
TAGCATTATATATTATTTTTTTATTAAGGAGCTCCGCTCCACAATATATTTTTATTTTAATTTTTTTTATATTATTTATA
AATAATAATTAGATGGTAAT

Downstream 100 bases:

>100_bases
TGTATTATATATTTATATTTATTGTTTTACTCTTTGTTTTTTTAATTTAACAAGTTTTTCTACTGCATTCATATTTACTC
CTTCTCCATATTCAACATAT

Product: cytidyltransferase-like protein

Products: diphosphate; CDPglycerol

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKTNDKTKKIVLTAGTFDLLHPGHHNTLKYAKSLGDELIVVIARDETVKKIKGRKPVIPENQRREMIEAIKPVDKAILGS
LTDKLEPILKIKPDIIVLGPDQITFDAENLKNELKKLGLDVEIVKTKEYTNCDFHSSYDIIKEIVKRWCNKKIIKK

Sequences:

>Translated_156_residues
MKTNDKTKKIVLTAGTFDLLHPGHHNTLKYAKSLGDELIVVIARDETVKKIKGRKPVIPENQRREMIEAIKPVDKAILGS
LTDKLEPILKIKPDIIVLGPDQITFDAENLKNELKKLGLDVEIVKTKEYTNCDFHSSYDIIKEIVKRWCNKKIIKK
>Mature_156_residues
MKTNDKTKKIVLTAGTFDLLHPGHHNTLKYAKSLGDELIVVIARDETVKKIKGRKPVIPENQRREMIEAIKPVDKAILGS
LTDKLEPILKIKPDIIVLGPDQITFDAENLKNELKKLGLDVEIVKTKEYTNCDFHSSYDIIKEIVKRWCNKKIIKK

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0615

COG function: function code MI; Cytidylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4505651, Length=126, Percent_Identity=34.1269841269841, Blast_Score=70, Evalue=8e-13,
Organism=Homo sapiens, GI296841136, Length=126, Percent_Identity=34.1269841269841, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789432, Length=144, Percent_Identity=34.0277777777778, Blast_Score=67, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550974, Length=101, Percent_Identity=40.5940594059406, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550975, Length=101, Percent_Identity=40.5940594059406, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45550973, Length=101, Percent_Identity=40.5940594059406, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2 [H]

EC number: 2.7.7.39

Molecular weight: Translated: 17826; Mature: 17826

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNDKTKKIVLTAGTFDLLHPGHHNTLKYAKSLGDELIVVIARDETVKKIKGRKPVIPE
CCCCCCCCEEEEEECCHHHCCCCCCHHHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCC
NQRREMIEAIKPVDKAILGSLTDKLEPILKIKPDIIVLGPDQITFDAENLKNELKKLGLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEECHHHHHHHHHHHCCC
VEIVKTKEYTNCDFHSSYDIIKEIVKRWCNKKIIKK
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTNDKTKKIVLTAGTFDLLHPGHHNTLKYAKSLGDELIVVIARDETVKKIKGRKPVIPE
CCCCCCCCEEEEEECCHHHCCCCCCHHHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCC
NQRREMIEAIKPVDKAILGSLTDKLEPILKIKPDIIVLGPDQITFDAENLKNELKKLGLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEECHHHHHHHHHHHCCC
VEIVKTKEYTNCDFHSSYDIIKEIVKRWCNKKIIKK
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; sn-glycerol 3-phosphate

Specific reaction: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]